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Egyptian Journal of Aquatic Biology and Fisheries
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A. Rashad, A., M. Gad Allaha, S., I. Ahmed, I., G. Shehata, M. (2019). Toxic and biological effects of Moringa oleifera Lam. crude seed extract against Culex pipiens L. (Diptera; Culicidae) larvae. Egyptian Journal of Aquatic Biology and Fisheries, 23(4), 117-125. doi: 10.21608/ejabf.2019.52172
Amina A. Rashad; Sohair M. Gad Allaha; Imam I. Ahmed; Magdi G. Shehata. "Toxic and biological effects of Moringa oleifera Lam. crude seed extract against Culex pipiens L. (Diptera; Culicidae) larvae". Egyptian Journal of Aquatic Biology and Fisheries, 23, 4, 2019, 117-125. doi: 10.21608/ejabf.2019.52172
A. Rashad, A., M. Gad Allaha, S., I. Ahmed, I., G. Shehata, M. (2019). 'Toxic and biological effects of Moringa oleifera Lam. crude seed extract against Culex pipiens L. (Diptera; Culicidae) larvae', Egyptian Journal of Aquatic Biology and Fisheries, 23(4), pp. 117-125. doi: 10.21608/ejabf.2019.52172
A. Rashad, A., M. Gad Allaha, S., I. Ahmed, I., G. Shehata, M. Toxic and biological effects of Moringa oleifera Lam. crude seed extract against Culex pipiens L. (Diptera; Culicidae) larvae. Egyptian Journal of Aquatic Biology and Fisheries, 2019; 23(4): 117-125. doi: 10.21608/ejabf.2019.52172

Toxic and biological effects of Moringa oleifera Lam. crude seed extract against Culex pipiens L. (Diptera; Culicidae) larvae

Article 10, Volume 23, Issue 4, October 2019, Page 117-125  XML PDF (450.63 K)
Document Type: Original Article
DOI: 10.21608/ejabf.2019.52172
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Authors
Amina A. Rashad; Sohair M. Gad Allaha; Imam I. Ahmed; Magdi G. Shehata
Abstract
Mosquitos' resistance to chemical insecticides motivated the need for alternative control strategy. Therefore, botanical extracts have become one of the controls of revolutionary methods in the sustainable vector strategy. The current study was conducted under laboratory conditions to evaluate the toxicological efficacy of the crude seed extract of Moringa oleifera against the 3rd larval instar of Culex pipiens mosquito. A series of aqueous concentrations had been prepared (100, 80, 60, 40 and 20 ppm) to carry out the laboratory bio-assay experiment. The highest larval mortality took place at 100 ppm concentration (81.39%). LC50 and LC90 values of M. oleifera seed extract recorded 67.91 ppm and 144.63 ppm after 120 hr exposure time, respectively. The obtained data revealed that larvicidal effect, malformed feature incidence and the cumulative larval death percentages were of a time and concentration-dependent manner. A similar trend had been documented for the total death percentages of both pupal and adult stages. On the contrary, the percentages of viable pupation and the emerged mosquito showed reverse responses. Accordingly, Moringa extract could be considered as a promising non-chemical candidate in the IPM programs of Culex mosquito.
Keywords
Moringa oleifera; Culex pipiens; Culicidae; larvicidal effect; Bioassay; Seed extract
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