• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Peer Review Process
  • Guide for Authors
  • Submit Manuscript
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Egyptian Journal of Aquatic Biology and Fisheries
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 29 (2025)
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Issue 1
Volume Volume 28 (2024)
Volume Volume 27 (2023)
Volume Volume 26 (2022)
Volume Volume 25 (2021)
Volume Volume 24 (2020)
Volume Volume 23 (2019)
Volume Volume 22 (2018)
Volume Volume 21 (2017)
Volume Volume 20 (2016)
Volume Volume 19 (2015)
Volume Volume 18 (2014)
Volume Volume 17 (2013)
Volume Volume 16 (2012)
Volume Volume 15 (2011)
Volume Volume 14 (2010)
Volume Volume 13 (2009)
Volume Volume 12 (2008)
Volume Volume 11 (2007)
Volume Volume 10 (2006)
Volume Volume 9 (2005)
Volume Volume 8 (2004)
Volume Volume 7 (2003)
Volume Volume 6 (2002)
Volume Volume 5 (2001)
Volume Volume 4 (2000)
Volume Volume 3 (1999)
Volume Volume 2 (1998)
Volume Volume 1 (1997)
et al., Z. (2025). Response of Aquatic Bacterial Species Isolated from Contaminaed Water to Whole Body Extract of Lucilia sericata Larvae. Egyptian Journal of Aquatic Biology and Fisheries, 29(1), 1181-1199. doi: 10.21608/ejabf.2025.409622
Zidan et al.. "Response of Aquatic Bacterial Species Isolated from Contaminaed Water to Whole Body Extract of Lucilia sericata Larvae". Egyptian Journal of Aquatic Biology and Fisheries, 29, 1, 2025, 1181-1199. doi: 10.21608/ejabf.2025.409622
et al., Z. (2025). 'Response of Aquatic Bacterial Species Isolated from Contaminaed Water to Whole Body Extract of Lucilia sericata Larvae', Egyptian Journal of Aquatic Biology and Fisheries, 29(1), pp. 1181-1199. doi: 10.21608/ejabf.2025.409622
et al., Z. Response of Aquatic Bacterial Species Isolated from Contaminaed Water to Whole Body Extract of Lucilia sericata Larvae. Egyptian Journal of Aquatic Biology and Fisheries, 2025; 29(1): 1181-1199. doi: 10.21608/ejabf.2025.409622

Response of Aquatic Bacterial Species Isolated from Contaminaed Water to Whole Body Extract of Lucilia sericata Larvae

Article 64, Volume 29, Issue 1, January and February 2025, Page 1181-1199  XML PDF (458.33 K)
DOI: 10.21608/ejabf.2025.409622
View on SCiNiTO View on SCiNiTO
Author
Zidan et al.
Abstract
      Drainage microbial contamination has been identified as a serious human health risk that have a detrimental impact on health issues brought on by the invasion of pathogenic organisms and are associated with wound infection. Ingestion or contact of polluted water are the two ways that these bacteria might infect humans. Maggot debridement is a commonly used treatment around the world to clean wounds. Based on these data, the aim of this work was to identify the microbial contamination, examining gene expression and possible antibacterial properties of metabolites from Extraction/Secretions (ES) of Lucilia sericata larvae. In this study, the antibacterial activity of the ES of L. sericata larvae was examined against Gram-positive Streptococcus pneumoniae, Staphylococcus aureus, and Gram-negative E. coli, Pseudomonas aeruginosa bacteria, both in their sterilized and multi-antibiotic-resistant forms. The agar well diffusion method was utilized to assess the maggots' ES in comparison with the strains. A 2-D PAGE protein analysis was performed. Inhibition zones were observed for S.aureus (18.3±2.1mm), Streptococcus pneumoniae (13.4±0.58mm) and E. coli (20.4±2.0mm); however, the extract was unable to produce an inhibiton zone for P.aeruginosa. Different proteins with varying molercular mass (<20-150kDa) and pI (3.3-7) were observed using 2-D PAGE. Following a series of antibiotic treatments, we assessed lucifensin and attacin gene expression changes in the bacteria. The antibacterial impact was investigated using antibiotic disk diffusion and optical absorption by analyzing the expression of the previously known genes. Using Fluorescence-activated cell sorting analysis, various extract dilutions showed varying killing rates for S. aureus, S. pneumoniae, E. coli, and P. aeruginosa, with killing rates of 76, 71, 89.2, and 49.1% for the lowest (1/64) dilution, respectively.
Keywords
Aquatic bacteria; Aquatic microbial; contaminants; SDS-PAGE; MDT; Lucilia sericata; Gene expression; Larval debridement; Extraction/secretions; Anti-microbial effects
Statistics
Article View: 111
PDF Download: 136
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by NotionWave.