Valuation Bio-Impacts of Different Doses for Bacillus Subtilis on Growth Rates, Some Tissues and Genes of the Shrimp Litopenaeus Vannamei

In the present study, five various treatment doses of Bacillus subtilis (BS) were examined associated with the growth rate, some tissues and genes for the Litopenaeus vannamei (L. vannamei). BS  was secluded from the intestine of farmed shrimp (L. vannamei) and then added to the shrimp’s diets in five doses of BS. Sixty shrimps were randomly divided into six groups as follows: group 1 (T0_a) as a control-farmed group, group 2 (T1), group 3 (T2), group 4 (T3), group 5 (T4) and Group 6 (T5). After two months of the experimental period, the T0_b group (control-natural group), which contained ten shrimps brought from the Mediterranean Sea, was determined as group 7. At the end of the two months, the growth rates were calculated for the first six groups, and the histological status in hepatopancreas, intestine, muscle, and antenna in addition to the genetics studies were assessed for the seven groups. The group which was treated with (4/5*10⁵ CFU of BS/g) showed the best results in weight gain, survival rate, increase in body length, and enhancement in the tissues of the hepatopancreas, intestine, muscle, antenna and genes of the shrimp. Therefore, this dose is recommended to be used in culturing shrimp in order to increase productivity and contribute to solving the food problem as one of the goals of sustainable development concerning “Egypt Vision 2030".