Lignocellulosic substrate as a low-cost effective inducer for production of hydrolytic cellulases by marine halophilic Aspergillus ochraceus

Alnusaire, Taghreed S.; Farag, Aida M.

doi:10.21608/ejabf.2022.262654

Lignocellulosic substrate as a low-cost effective inducer for production of hydrolytic cellulases by marine halophilic Aspergillus ochraceus

Document Type : Original Article

Authors

10.21608/ejabf.2022.262654

Abstract

Enzymes are one of the key materials widely recognized for their diverse applications in industry. The cellulase enzymes are used for many industrial applications such as in food, paper, biofuel, textile, and industries. The cellulolytic potential of a marine isolate, Aspergillus ochraceus was studied by growing it on different carbon and nitrogen sources. The impact of the introduction of lignocellulosic substrates separately into the fermentation medium on enzyme productivity was studied. Results revealed that Aspergillus ochraceus produced maximum activity of Fpase and CMCase in the presence of wheat bran as a sole carbon source under submerged fermentation compared to solid-state fermentation after 3 days of incubation. Statistical screening of cultural conditions for the highest enzyme production by marine halophilic Aspergillus ochraceus was carried out using Plackett–Burman statistical design. The design showed that the optimized media enhanced CMCase and Fpase activities by 2.87 and 2.17-fold, respectively, higher than that recorded with the basal cultural conditions.

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