A. A. Abdrabo, M., A. H. Ibrahim, H., Hassan, S., M. Abdul-Raouf, U. (2018). Antimicrobial and anti-tumor activities of exopolysaccharides produced by the biofilm of marine Halomonas saccharevitans AB2 isolated from Suez Gulf, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 22(5 (Special Issue)), 99-119. doi: 10.21608/ejabf.2018.22007
Mohamed A. A. Abdrabo; Hassan A. H. Ibrahim; SaharHassan WefkyMostafa Hassan; Usama M. Abdul-Raouf. "Antimicrobial and anti-tumor activities of exopolysaccharides produced by the biofilm of marine Halomonas saccharevitans AB2 isolated from Suez Gulf, Egypt". Egyptian Journal of Aquatic Biology and Fisheries, 22, 5 (Special Issue), 2018, 99-119. doi: 10.21608/ejabf.2018.22007
A. A. Abdrabo, M., A. H. Ibrahim, H., Hassan, S., M. Abdul-Raouf, U. (2018). 'Antimicrobial and anti-tumor activities of exopolysaccharides produced by the biofilm of marine Halomonas saccharevitans AB2 isolated from Suez Gulf, Egypt', Egyptian Journal of Aquatic Biology and Fisheries, 22(5 (Special Issue)), pp. 99-119. doi: 10.21608/ejabf.2018.22007
A. A. Abdrabo, M., A. H. Ibrahim, H., Hassan, S., M. Abdul-Raouf, U. Antimicrobial and anti-tumor activities of exopolysaccharides produced by the biofilm of marine Halomonas saccharevitans AB2 isolated from Suez Gulf, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 2018; 22(5 (Special Issue)): 99-119. doi: 10.21608/ejabf.2018.22007
Antimicrobial and anti-tumor activities of exopolysaccharides produced by the biofilm of marine Halomonas saccharevitans AB2 isolated from Suez Gulf, Egypt
1Marine Microbiology Dept., National Institute of Oceanography and Fisheries, Alexandria, Egypt
2National Institute of Oceanography and Fisheries (NIOF), Marine MicrobiologyDep.,Kayet Bay, El-Anfushy, Alexandria, Egypt.
3Faculty of Science, Al-Azhar University, Assiut Branch, Egypt
Abstract
Thirteen different attached marine bacteria were isolated from the surface of algae, rocksin biofilm forming device in Red Sea. They were screened for their potentiality in the exopolysaccharides (EPS) production. The most potent marine isolate 2D was identified by 16S rDNA analysis as Halomonas saccharevitans AB2 with 99% similarity. Optimum culture conditions which ease the synthesis of EPSsfrom Halomonas saccharevitans AB2 were evaluated. It grew well at 25˚C, pH 9 and on potato sea water extract with concentration 100%, lactose and malt extract as the best carbon and nitrogen sources. Application of Plackett-Burman experimental design achieved the maximum yield of EPSs (138 gl-1) with 1.4 fold increase when compared to growth under the basal conditions. The maximum yield of EPSs (153gl-1) with 1.6 fold increase was obtained upon applying Box-Behnken design. The EPSs showed antimicrobial activity by inhibition of different pathogenic bacteria and fungi with the maximum absolute unit (AU= 14.1 and 25.1) against Vibrio fluvialis and Aspergillus niger ATCC 16404, respectively. Moreover, these EPSs exhibited activities as antitumor agent against 8 cell lines with maximum inhibition (87%) at 50 μg with IC50 =20.3 μgml-1against human lung carcinoma (A-549). Potential antimicrobial and antitumor activities property of EPSs produced by H. saccharevitans AB2 may lead to the development of novel medical drugs.
View on SCiNiTO
Top