Abdelhamid1, A., El-Barbary, M., Mabrouk, E. (2013). Bacteriological status of ashtoum El-Gamil protected area. Egyptian Journal of Aquatic Biology and Fisheries, 17(3), 11-23. doi: 10.21608/ejabf.2013.2173
Abdelhamid Abdelhamid1; Manal El-Barbary; El-Deweny Mabrouk. "Bacteriological status of ashtoum El-Gamil protected area". Egyptian Journal of Aquatic Biology and Fisheries, 17, 3, 2013, 11-23. doi: 10.21608/ejabf.2013.2173
Abdelhamid1, A., El-Barbary, M., Mabrouk, E. (2013). 'Bacteriological status of ashtoum El-Gamil protected area', Egyptian Journal of Aquatic Biology and Fisheries, 17(3), pp. 11-23. doi: 10.21608/ejabf.2013.2173
Abdelhamid1, A., El-Barbary, M., Mabrouk, E. Bacteriological status of ashtoum El-Gamil protected area. Egyptian Journal of Aquatic Biology and Fisheries, 2013; 17(3): 11-23. doi: 10.21608/ejabf.2013.2173
Bacteriological status of ashtoum El-Gamil protected area
1Dept. of Animal Production, Fac. of Agriculture, Mansoura Univ., Mansoura, Egypt
2Fish Pathology Lab., National Institute for Oceanography and Fisheries, Cairo, Egypt.
Abstract
Survey study was carried out throughout four seasons from four locations representing the whole protected area "Ashtoum El-Gamil", Port Said governorate, Manzala Lake concerning the bacterial load in water and fish of this park. The bacterial load in water samples increased by increasing water temperature, the range of total count of bacteria was 0.6×106 to 0.2×108 /100 ml of water sample in winter and summer respectively. The following bacterial genera were isolated from water samples: Aeromonas, Bacillus, Proteus, Pseudomonas, Streptococcus, S. epidermidis, S. aureus, Micrococcus, Vibrio and E. coli. The predominant microorganisms isolated from water were E. coli, Bacillus spp., Vibrio spp. and Pseudomonas spp. While the total bacterial counts (TBCs) from fish samples obtained on NA media were higher in summer season in all tested organs than other seasons (spring, winter and autumn); also, the high bacterial load was observed in both of gills and intestine comparing with the liver. TBCs were 0.8×107, 0.52×107 and 0.13×107 cfu / g in gills, intestine and liver, respectively in location No. 3 (bahr kassab) during the summer. The highest TBCs obtained on McCA, MSA, and BHA media were 0.16×107, 0.25×107, and 0.3×107 cfu / g of gills in locations No. 1, 1 and 3 during summer, autumn and summer, respectively. The bacterial species isolated also from fish were namely V. cholerae, V. parahaemolyticus, Aeromonas spp., E. coli, Yersinia spp., Proteus, Pseudomonas spp., Micrococcus spp., S. epidermidis, S. aureus and Streptococcus spp. In the pathogenicity test, the most pathogenic bacteria for tilapia fish were Pseudomonas spp., V. cholerae and Aeromonas spp which causing greater than 70, 60% and 60% mortality within 96 hr respectively.
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