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Egyptian Journal of Aquatic Biology and Fisheries
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Sabri, D., Rabie, T., Ahmed, A., Zakaria, S., Bourdineaud, J. (2013). Zebrafish ABCC5 gene expression in relation to metallic contamination and presence of Tubifex worms. Egyptian Journal of Aquatic Biology and Fisheries, 17(1), 1-12. doi: 10.21608/ejabf.2013.2151
Dalia Sabri; Tarik Rabie; Ashraf Ahmed; Saad Zakaria; Jean- Bourdineaud. "Zebrafish ABCC5 gene expression in relation to metallic contamination and presence of Tubifex worms". Egyptian Journal of Aquatic Biology and Fisheries, 17, 1, 2013, 1-12. doi: 10.21608/ejabf.2013.2151
Sabri, D., Rabie, T., Ahmed, A., Zakaria, S., Bourdineaud, J. (2013). 'Zebrafish ABCC5 gene expression in relation to metallic contamination and presence of Tubifex worms', Egyptian Journal of Aquatic Biology and Fisheries, 17(1), pp. 1-12. doi: 10.21608/ejabf.2013.2151
Sabri, D., Rabie, T., Ahmed, A., Zakaria, S., Bourdineaud, J. Zebrafish ABCC5 gene expression in relation to metallic contamination and presence of Tubifex worms. Egyptian Journal of Aquatic Biology and Fisheries, 2013; 17(1): 1-12. doi: 10.21608/ejabf.2013.2151

Zebrafish ABCC5 gene expression in relation to metallic contamination and presence of Tubifex worms

Article 1, Volume 17, Issue 1, January 2013, Page 1-12  XML PDF (290.17 K)
Document Type: Original Article
DOI: 10.21608/ejabf.2013.2151
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Authors
Dalia Sabri1; Tarik Rabie2; Ashraf Ahmed3; Saad Zakaria3; Jean- Bourdineaud4
1Biotechnology Research Center (BRC), Suez Canal University, Ismailia 41522, Egypt.
2Department of Animal Production, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt.
3Department of Marine Science, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt.
4Bordeaux University, CNRS, UMR EPOC 5805, Arcachon Marine Station, Arcachon 33120, France.
Abstract
The impact of contaminated sediment with copper and cadmium on the expression of ABCC5 gene expression in Zebrafish Danio rerio was studied after seven days of exposure. Also the effect of Tubifex worms as bioturbator organism on the gene expression was investigated. Expression of ABCC5 gene was estimated in Zebrafish brain, gills, muscles and digestive tract tissues in four different experimental conditions. The highest expression for ABCC5 gene was found in brain samples of all tested groups, while the gene expression in gills didn’t show any significant change in all groups. Presence of Cu and Cd had led to a significant increase in the expression of ABCC5 gene in brain and digestive tract samples. Surprisingly, in presence of Tubifex worms, expression of the same gene was higher in zebrafish tissues than expression in normal condition in absence of Tubifex worms. The only exception was in gills tissues, where a lower expression was detected.
Keywords
ABCC5 gene; Danio rerio; Heavy metals; Lake Manzala; Tubifex tubifex
Main Subjects
Fisheries
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