S. Yaseen, M., Abdelaziz, M., A. Abdel-moneam, D., Abd-Elhay, E., M. Wassif, I., Moustafa, M. (2020). Efficacy of Dietary Nucleotides (Nucleoforce™)on growth, haemato-immunological response and disease resistance in Pangasianodon hypophthalmus fish (Sauvage, 1878) in Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 24(6), 405-424. doi: 10.21608/ejabf.2020.112821
Mai S. Yaseen; Mohamed Abdelaziz; Dalia A. Abdel-moneam; Elsayed Abd-Elhay; Islam M. Wassif; Mohamed Moustafa. "Efficacy of Dietary Nucleotides (Nucleoforce™)on growth, haemato-immunological response and disease resistance in Pangasianodon hypophthalmus fish (Sauvage, 1878) in Egypt". Egyptian Journal of Aquatic Biology and Fisheries, 24, 6, 2020, 405-424. doi: 10.21608/ejabf.2020.112821
S. Yaseen, M., Abdelaziz, M., A. Abdel-moneam, D., Abd-Elhay, E., M. Wassif, I., Moustafa, M. (2020). 'Efficacy of Dietary Nucleotides (Nucleoforce™)on growth, haemato-immunological response and disease resistance in Pangasianodon hypophthalmus fish (Sauvage, 1878) in Egypt', Egyptian Journal of Aquatic Biology and Fisheries, 24(6), pp. 405-424. doi: 10.21608/ejabf.2020.112821
S. Yaseen, M., Abdelaziz, M., A. Abdel-moneam, D., Abd-Elhay, E., M. Wassif, I., Moustafa, M. Efficacy of Dietary Nucleotides (Nucleoforce™)on growth, haemato-immunological response and disease resistance in Pangasianodon hypophthalmus fish (Sauvage, 1878) in Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 2020; 24(6): 405-424. doi: 10.21608/ejabf.2020.112821
Efficacy of Dietary Nucleotides (Nucleoforce™)on growth, haemato-immunological response and disease resistance in Pangasianodon hypophthalmus fish (Sauvage, 1878) in Egypt
A feeding trial was performed to evaluate the efficacy of Dietary Nucleotides (Nucleoforce™) on Growth performance, haematological, biochemical, Immunological indices and disease resistance of Pangasianodon hypophthalmus fish to Pseudomonas aeruginosa. Dietary nucleotides were incorporated in the diet at different concentrations; group I control, group II 250 g/ ton diet and group III 500 g/ ton diet for 8 weeks. At the end of the feeding trial, weight gain, and specific growth rate (SGR) were recorded. Results showed that there was a significant increase in fish groups received diet supplemented with nucleotides in comparison with the control group. Haematogram indices showed a significant elevation in RBCs count, HB value, WBCs, and leukocytic count. Biochemically, High superoxide dismutase (SOD) activity, total protein and globulin levels were recorded in fish fed on 500g/t nucleotide (NT). Immunologically, Lymphocytic proliferation activity, Nitric oxide concentration and Serum lysozyme activity were continuously increased in 250g/t and the 500g/t NT treatment groups along feeding duration. At the end of 8th week, the experimentally reared fish were challenged intraperitoneally with virulent strain of Pseudomonas aeruginosa (0.2 ml of 3× 107 CFU) and the cumulative mortalities were recorded to be lower in nucleotide supplemented groups compared to the control group. In conclusion, the supplementation of fish diet with nucleotides in concentration of 500g/t nucleotide can improve the general health status of P. hypophthalmus via increasing the disease resistance and minimizing stressful conditions.
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