Establishment of a cell culture system and characterization of the primary cultures from different organs of the Nile tilapia Oreochromis niloticus

H. Hashem, Medhat; T. Meshhal, Dina; A. S. Mohamed, Fatma; A. Khamiss, Omaima

doi:10.21608/ejabf.2020.96005

Establishment of a cell culture system and characterization of the primary cultures from different organs of the Nile tilapia Oreochromis niloticus

Document Type : Original Article

Authors

10.21608/ejabf.2020.96005

Abstract

In addition to toxicology, carcinogenesis, functional genomics studies, and lacking in vivo complexities, fish cell culture considered necessary to study virus-host interactions and isolate viral pathogens in fishes. Therefore, the present study aimed to establish a cell culture system and characterize the primary culture from different organs of Nile tilapia Oreochromis niloticus fish. Leibovitz-15 (L-15) medium containing L. glutamine and supplemented by 15% of Foetal Bovine Serum (FBS), 200 IU/ml penicillin, 200 µg/ml streptomycin and 2.5 µg/ml amphotericin were used for culturing the cells. In this study, Primary cultures from different organs (brain, testis, and ovaries) from Oreochromis niloticus were established. The results of the present investigations showed that brain primary cultures grew slowly. Active growth was observed in ovary primary culture on the 7^th day with fibroblast-like growth. Testis primary culture showed active growth on the 2^nd day and was capable of maintaining organ function.

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