Preliminary bioassay on antibacterial effects of Tripneustes gratilla extracts from the Red Sea, Egypt

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Abstract

Tripneustes gratilla are entirely depended on powerful innate immune systems in order to stay healthy. An attempt is conducted to evaluate the antibacterial effects of extracts obtained from different tissues (i.e. gonad, gut, and spine) of the sea urchin T.gratilla from the Red Sea against some selected bacterial pathogens. Potentially bioactive metabolites extracted from different organs, using methanol and chloroform, were examined against seven pathogenic bacterial species; Staphylococcus aureus 25923, Enterococcus faecalis 29212, Pseudomonas aeruginosa 9027, Bacillus subtilis 6633, Escherichia coli 8739, Vibrio fluvialis and Vibrio damsela. The methanolic gut extract showed the highest antibacterial activity against the examined pathogenic bacteria. The minimum inhibitory concentration of the methanolic gut extract was determined at different concentrations (200- 1000 μg / ml) using the broth dilution method. FTIR and GC-MS were determined. The study indicated that the sea urchin T. gratilla gut extract seems to be a promising source of antibacterial compounds. 

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