Genetic Structure and Phylogenetic Relationships of the Mitre Squid Uroteuthis chinensis (Gray, 1849) (Myopsida: Loliginidae) from Bangka-Belitung Islands

Robin, Robin; Anjani, Tiara Puspa; Simanjuntak, Susi Watina

doi:10.21608/ejabf.2025.433255.6810

Genetic Structure and Phylogenetic Relationships of the Mitre Squid Uroteuthis chinensis (Gray, 1849) (Myopsida: Loliginidae) from Bangka-Belitung Islands

Document Type : Original Article

Authors

¹ 1 Department of Aquaculture, Agriculture, Fisheries and Marine Faculty, Universitas Bangka Belitung, Jl Kampus Terpadu UBB, Balunijuk 33127, Bangka Belitung, Indonesia.

² Department of Aquaculture, Faculty of Agriculture, Fisheries and Marine, Universitas Bangka Belitung, Jl Kampus Terpadu UBB, Balunijuk 33127, Bangka Belitung, Indonesia.

³ Department of Capture Fisheries, Faculty of Agriculture, Fisheries and Marine, Universitas Bangka Belitung, Jl Kampus Terpadu UBB, Balunijuk 33127, Bangka Belitung, Indonesia.

10.21608/ejabf.2025.433255.6810

Abstract

This study examines the genetic structure and phylogenetic relationships of Uroteuthis chinensis (Gray, 1849) in Bangka Belitung waters, Indonesia, using mitochondrial cytochrome c oxidase subunit I (COI) gene sequences. Twenty-four sequences were analyzed, comprising four new field-collected samples and 20 GenBank references. Alignment with ClustalW in MEGA X produced a consensus length of 446 bp. BLAST analysis confirmed species identity, showing ≥99% similarity with U. chinensis references. Nucleotide composition revealed an AT-rich profile (61.6% AT content), consistent with typical mitochondrial DNA of marine invertebrates. Genetic diversity analysis identified eight haplotypes, with haplotype diversity (Hd) of 0.681 and nucleotide diversity (π) of 0.00219, indicating moderate diversity with low nucleotide differences. Eight polymorphic sites were found, including seven singleton variable sites and one parsimony-informative site. Pairwise genetic distances (K2P model) ranged from 0.002 to 0.009, suggesting low to moderate intraspecific variation. Neighbor-joining phylogeny with 1,000 bootstrap replicates revealed several clusters (bootstrap 28–50), indicating localized genetic diversification. These findings suggest U. chinensis populations in Bangka Belitung are genetically homogeneous but retain unique haplotypes relevant to population dynamics and local adaptation. Broader geographic sampling and additional genetic markers are recommended to clarify population structure and connectivity in Indonesian waters.

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