Morphological and Molecular-Genetic Characterization of the Nematode Oswaldocruzia filiformis Goeze, 1782 (Nematoda: Molineoidea) in the Amphibians of Southern and Central Uzbekistan

Et Al., Aliyev

doi:10.21608/ejabf.2025.428720.6697

Morphological and Molecular-Genetic Characterization of the Nematode Oswaldocruzia filiformis Goeze, 1782 (Nematoda: Molineoidea) in the Amphibians of Southern and Central Uzbekistan

Document Type : Original Article

Author

Aliyev et al.

10.21608/ejabf.2025.428720.6697

Abstract

In this article, the morphological and molecular-genetic analysis of the nematode Oswaldocruzia filiformis Goeze, 1782, found in amphibians of Southern and Central Uzbekistan, was conducted. As a result of molecular-genetic analyses, 419 nucleotide pairs of the mitochondrial DNA coi region were isolated and sequenced using PCR amplification. Consequently, the nematode O. filiformis_uz was compared with NCBI data and confirmed to match 99.5% with O. filiformis (MT300275). The obtained data and nucleotide analysis of species belonging to this genus in the NCBI database demonstrated that this species is a morphologically and molecular-genetically stable single species. Morphological and morphometric analyses revealed that the body of the nematode O. filiformis is thin, elongated, with a medium-width body, and the cephalic vesicles are located in the vesicle at the anterior end. The body length of male nematodes ranges from 6.3 to 8.2mm, with a width of 0.16 to 0.17mm, and their oral structure does not include lips. The esophagus length is 0.39 to 0.47mm, and the caudal bursa consists of three lobes and their ribs. The length of the spicules is 0.19 to 0.25mm, and the gubernaculum is composed of several parts. Female nematodes are slightly larger, with a body length of 14.2 to 18.6mm, a width of 0.21 to 0.25mm, and an esophagus length of 0.46 to 0.49mm. The ovary was found to form rings in the anterior part of the uterus.

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