In vitro Cytotoxicity of Plantaricin MS9 Produced by Lactiplantibacillus plantarum MS, Isolated from the Mediterranean Sea, Egypt

Elnaggar, Moustafa; Lamada, Mennatullah; Hamdan, Amira

doi:10.21608/ejabf.2025.429370.6710

In vitro Cytotoxicity of Plantaricin MS9 Produced by Lactiplantibacillus plantarum MS, Isolated from the Mediterranean Sea, Egypt

Document Type : Original Article

Authors

¹ Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt

² Botany and Microbiology Department, Faculty of Science, Alexandria University

³ Oceanography Department, faculty of Science, Alexandria University

10.21608/ejabf.2025.429370.6710

Abstract

The present study evaluates the cytotoxic activity of the bacteriocin
produced from Lactiplantibacillus plantarum MS, a novel marine lactic acid
bacterium. The results showed that the crude bacteriocin displayed a wide
antibacterial spectrum against some selected bacterial strains. The purified
bacteriocin has a molecular weight of 3.5 kDa detected by SDS-PAGE and a
molecular mass of 2995.6 Da quantified by LC/MS. Based on amino acid
sequencing, it was concluded that plantaricin MS9 is a new class IId
bacteriocin with a bacteriostatic mode of action. Biochemical
characterization of plantaricin MS9 confirmed that it is thermostable over a
broad range of temperatures (50- 100°C), retained its activity at acidic and
alkaline pH values, and was strongly inhibited by proteolytic enzymes.
Furthermore, plantaricin MS9 exhibited strong antiproliferative activity and
detrimental effects against three cancer cell lines: colon cancer (Caco-2),
liver cancer (HepG2), and breast cancer (Mcf-7), with inhibition rates of 93,
95, and 91%, respectively. In conclusion, the collected data revealed that
plantaricin MS9 is of potential interest for future applications in the
pharmaceutical industry.

Keywords