Enhancement of Gonadal Maturity in Female Sand Lobster Panulirus homarus Using Shortwave Radiation Beams (Laser Puncture)

Fadjar, Mohamad; Hariani, Dyah; AMRILLAH, ATTABIK MUKHAMMAD; Hadiana, Hadiana

doi:10.21608/ejabf.2025.424482.6593

Enhancement of Gonadal Maturity in Female Sand Lobster Panulirus homarus Using Shortwave Radiation Beams (Laser Puncture)

Document Type : Original Article

Authors

¹ Aquaculture Dept., Fac. of Fisheries and Marine Science, University of Brawijaya

² Universitas Negeri Surabaya

³ Study Program of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Universitas Brawijaya, East Java, Indonesia

⁴ Universitas Brawijaya

10.21608/ejabf.2025.424482.6593

Abstract

Panulirus homarus, an important export commodity, faces challenges in mastering artificial breeding techniques, with limited practical applications despite advancements in seed production. This study investigated the effects of laser exposure on gonadal maturation, gene expression (Molting-Inhibiting Hormone, MIH), and hormone levels (17β-estradiol) in P. homarus. A total of 50 female broodstock were acclimated for 7 days and exposed to a soft He-Ne laser for 4, 6, or 8 seconds. Gonadal maturity was assessed using the gonadal maturity level (GML) and gonadal maturity index (GMI), while MIH gene expression was quantified by quantitative PCR, and 17β-estradiol levels were measured using ELISA. Result revealed that laser exposure significantly influenced gonadal maturation and gene expression, with the 4-second exposure resulting in a 2.68-fold increase in MIH expression, and the 8-second exposure showing the highest increase at 4.21-fold. Both exposure groups exhibited advanced gonadal stages, with the 8-second exposure achieving the most mature gonads (GML 3). Serum 17β-estradiol levels were significantly higher in both the 4-second (2763.36 pg/mL) and 8-second (130.64 pg/mL) groups compared to controls. These findings suggest that laser exposure has a dose-dependent effect on gonadal maturation, MIH expression, and 17β-estradiol production. The results highlight the potential of laser technology as a biostimulant to enhance reproductive processes and optimize breeding practices in aquaculture.

Keywords