A Facile RP-HPLC Method for Quantification of Antiviral Drug Daclatasvir Dihydrochloride in Both Tablet Dosage Form and River Nile Samples

Et Al., Mohammed

doi:10.21608/ejabf.2025.427072.6646

A Facile RP-HPLC Method for Quantification of Antiviral Drug Daclatasvir Dihydrochloride in Both Tablet Dosage Form and River Nile Samples

Document Type : Original Article

Author

Mohammed et al.

10.21608/ejabf.2025.427072.6646

Abstract

A rapid and sensitive RP-HPLC method was developed for the quantitative analysis of daclatasvir dihydrochloride in tablet dosage forms. The method utilizes a Zorbax Eclipse Plus C18 column and a mobile phase consisting of 0.02M KH₂PO₄ buffer (pH 3.0) and acetonitrile (70:30% v/v). The method demonstrated excellent linearity (R² > 0.9996) over the concentration range of 0.15- 90µg/ mL, with low limits of detection (LOD) and quantification (LOQ). The method was also accurate and precise, with recovery values ranging from 98.72 to 101.30% and relative standard deviations less than 2.0%. In conclusion, this validated RP-HPLC method provides a reliable and efficient tool for the routine analysis of daclatasvir dihydrochloride in tablet formulations and the Nile River samples. This method can be applied for various quality control purposes, including dissolution testing and biowaiver studies.

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