Efficacy of Aeromonas veronii bv veronii BmSG-03 Feed-Based Vaccine on the Immunity of North African Catfish (Clarias gariepinus (Burchell 1822))

Mulia, Dini Siswani; Chasanah, Faydah Zukhruf; Purbomartono, Cahyono; Suwarsito, Suwarsito

doi:10.21608/ejabf.2025.424227.6590

Efficacy of Aeromonas veronii bv veronii BmSG-03 Feed-Based Vaccine on the Immunity of North African Catfish (Clarias gariepinus (Burchell 1822))

Document Type : Original Article

Authors

¹ Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Purwokerto

² Department of Aquaculture, Faculty of Agriculture and Fisheries, Universitas Muhammadiyah Purwokerto, Indonesia

³ Department of Aquaculture, Faculty of Agriculture and Fisheries, Universitas Muhammadiyah Purwokerto

10.21608/ejabf.2025.424227.6590

Abstract

Motile Aeromonas septicemia, caused by Aeromonas veronii bv. veronii poses a serious threat to freshwater aquaculture. This study evaluated the efficacy of A. veronii bv veronii BmSG-03 feed-based vaccine on the immunity of North African catfish (Clarias gariepinus Burchell 1822). An experimental approach was conducted using a completely randomized design (CRD) with five treatments and three replications. The treatments consisted of: P0 (control, without vaccination); P1 (feed-based vaccine for three consecutive days [days 1–3], with a booster on days 14–16); P2 (vaccine for four consecutive days [days 1–4], with a booster on days 14–17); P3 (vaccine for five consecutive days [days 1–5], with a booster on days 14–18); and P4 (vaccine for seven consecutive days [days 1–7], with a booster on days 14–20). Each experimental unit comprised ten catfish. The main parameters measured were antibody titer and survival rate, while supporting parameters included water temperature, pH, and dissolved oxygen. Primary parameter data were analyzed using analysis of variance (ANOVA) followed by Duncan’s multiple range test (DMRT) at a 5% significance level, whereas supporting parameters were analyzed descriptively. The results demonstrated that the A. veronii bv. veronii BmSG-03 feed-based vaccine was effective in increasing the immunity of North African catfish, enhancing both antibody titers and survival rates (P< 0.05) compared with the control. Among the treatments, administration of the vaccine for seven days followed by a seven-day booster (P5) was the most effective. These findings suggest that feed-based vaccines of A. veronii bv. veronii represent a promising immunoprophylactic strategy for freshwater aquaculture. Moreover, feed-based vaccination offers a practical, economical, and environmentally friendly approach to improving fish health, productivity, and sustainability, particularly in preventing Motile Aeromonas Septicemia in North African catfish farming.

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