Isolation and Identification of Monoculture Biofloc Forming Bacteria Alteromonas macleodii from Sea Water Using a Novel Isolation Method

Arnepalli, Chandrasekhar; Chapara, Manjulatha

doi:10.21608/ejabf.2025.445746

Isolation and Identification of Monoculture Biofloc Forming Bacteria Alteromonas macleodii from Sea Water Using a Novel Isolation Method

Authors

10.21608/ejabf.2025.445746

Abstract

Biofloc technology is an innovative and sustainable method used in aquaculture systems, playing a key role in maintaining water quality and assimilating residual organic matter in culture ponds. However, current biofloc studies primarily focus on microbial community assessments and the physicochemical characteristics of biofloc, with limited emphasis on monoculture-based biofloc studies. To address this gap, the present research was conducted. In this study, a novel monoculture biofloc-forming marine bacterium, F-504 (Alteromonas macleodii), was isolated from the coastal waters of Visakhapatnam using an innovative approach. This involved formulating a specialized growth medium and developing a new method for biofloc production using seawater. Four bacterial isolates were obtained during the study, among which isolate F-504 (Alteromonas macleodii) demonstrated positive results for monoculture biofloc formation. It was subsequently identified through both phenotypic and molecular characterization, and its sequence was later deposited in the NCBI GenBank. The findings of this study demonstrate the effectiveness of the proposed approach in discovering monoculture biofloc-forming bacteria and pave the way for identifying novel biofloc-producing organisms in future research.

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