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Egyptian Journal of Aquatic Biology and Fisheries
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Samara, S., Sa’diyah, H., Sari, C., Sari, L., Abualreesh, M. (2024). Effect of Fermented Mother Liquor (FML) Substitution Enriched with NaNO3 as a Chlorella vulgaris Culture Media. Egyptian Journal of Aquatic Biology and Fisheries, 28(5), 2323-2338. doi: 10.21608/ejabf.2024.389809
Syifania Samara; Halimatus Sa’diyah; Chintya Sari; Luthfiana Sari; Muyassar Abualreesh. "Effect of Fermented Mother Liquor (FML) Substitution Enriched with NaNO3 as a Chlorella vulgaris Culture Media". Egyptian Journal of Aquatic Biology and Fisheries, 28, 5, 2024, 2323-2338. doi: 10.21608/ejabf.2024.389809
Samara, S., Sa’diyah, H., Sari, C., Sari, L., Abualreesh, M. (2024). 'Effect of Fermented Mother Liquor (FML) Substitution Enriched with NaNO3 as a Chlorella vulgaris Culture Media', Egyptian Journal of Aquatic Biology and Fisheries, 28(5), pp. 2323-2338. doi: 10.21608/ejabf.2024.389809
Samara, S., Sa’diyah, H., Sari, C., Sari, L., Abualreesh, M. Effect of Fermented Mother Liquor (FML) Substitution Enriched with NaNO3 as a Chlorella vulgaris Culture Media. Egyptian Journal of Aquatic Biology and Fisheries, 2024; 28(5): 2323-2338. doi: 10.21608/ejabf.2024.389809

Effect of Fermented Mother Liquor (FML) Substitution Enriched with NaNO3 as a Chlorella vulgaris Culture Media

Article 132, Volume 28, Issue 5, September and October 2024, Page 2323-2338  XML PDF (515.57 K)
DOI: 10.21608/ejabf.2024.389809
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Authors
Syifania Samara; Halimatus Sa’diyah; Chintya Sari; Luthfiana Sariorcid ; Muyassar Abualreesh
Abstract
Chlorella vulgaris is a natural feed that plays an important role in cultivation due to its ease of cultivation and high nutritional value. However, its production is hindered by the high cost of the culture media, which makes the overall production expensive. One potential solution is to use a medium made from fermented mother liquor (FML) enriched with NaNO3, as its nitrogen-phosphorus (N/P) content is beneficial for the growth of C. vulgaris. This study aimed to assess the impact of FML and NaNO3 substitution as a culture medium for C. vulgaris and to determine the optimal dose. The treatments used in this study were P1 (Walne, serving as the control with an N/P ratio of 17:1), P2 (10:1), P3 (15:1), P4 (17:1), and P5 (20:1). The results demonstrated that media with FML substitution enriched with NaNO3 can not only replace Walne fertilizer but also outperform it. The optimal dose of FML substitution enriched with NaNO3 for maximizing chlorophyll-a, chlorophyll-b, and carotenoid content in C. vulgaris on a laboratory scale was found to be the N/P ratio of 15:1, with a population density of 10.92 x 106 cells/ml (day 7), a growth rate of 25 x 106 cells/ml (day 3), chlorophyll-a content of 0.49μg/ ml, chlorophyll-b of 0.46μg/ ml (day 7) and carotenoids of 0.16μg/ ml (day 7).
Keywords
Culture; Enrichment; Phytoplankton; Production
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