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Egyptian Journal of Aquatic Biology and Fisheries
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et al., S. (2024). Ethyl Acetate Extract of Jatropha curcas L. leaves as an Inhibitor of Histamine-Forming Bacterium in Fish Scombroid. Egyptian Journal of Aquatic Biology and Fisheries, 28(5), 1663-1677. doi: 10.21608/ejabf.2024.387002
Setha et al.. "Ethyl Acetate Extract of Jatropha curcas L. leaves as an Inhibitor of Histamine-Forming Bacterium in Fish Scombroid". Egyptian Journal of Aquatic Biology and Fisheries, 28, 5, 2024, 1663-1677. doi: 10.21608/ejabf.2024.387002
et al., S. (2024). 'Ethyl Acetate Extract of Jatropha curcas L. leaves as an Inhibitor of Histamine-Forming Bacterium in Fish Scombroid', Egyptian Journal of Aquatic Biology and Fisheries, 28(5), pp. 1663-1677. doi: 10.21608/ejabf.2024.387002
et al., S. Ethyl Acetate Extract of Jatropha curcas L. leaves as an Inhibitor of Histamine-Forming Bacterium in Fish Scombroid. Egyptian Journal of Aquatic Biology and Fisheries, 2024; 28(5): 1663-1677. doi: 10.21608/ejabf.2024.387002

Ethyl Acetate Extract of Jatropha curcas L. leaves as an Inhibitor of Histamine-Forming Bacterium in Fish Scombroid

Article 97, Volume 28, Issue 5, September and October 2024, Page 1663-1677  XML PDF (589.37 K)
DOI: 10.21608/ejabf.2024.387002
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Author
Setha et al.
Abstract
Jatropha curcas is known for its antimicrobial activity against various bacterial species, including Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Clostridium perfringens. This study investigated the effects of ethyl acetate extract from Jatropha curcas leaves as an inhibitor of histamine-forming bacterium. The antibacterial activity was assessed by evaluating cell component leakage and observing morphological and structural changes in bacterial cells. Cell leakage induced by the extract was quantified using spectrophotometry at wavelengths of 260 and 280nm. The results demonstrate significant morphological and structural alterations in bacterial cells, as evidenced by the scanning electron microscopy (SEM). High absorbance values in the supernatant indicate substantial loss of nucleic acids, exceeding that of proteins. SEM analysis revealed disrupted cell division in Klebsiella pneumoniae, cell wall lysis in Enterobacter aerogenes, and abnormal cell wall structures in Clostridium perfringens.
Keywords
Jatropha curcas L; Antibacterial; Histamine; Lysis
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