Recent Advances in Diagnosis of Pseudomonas Septicemia in Relation to Isopods Infestation in Pomadasys stridense

Qorany, Rehab Abdel Moneim; Mansour, Shimaa Mohamed

doi:10.21608/ejabf.2023.329194

Recent Advances in Diagnosis of Pseudomonas Septicemia in Relation to Isopods Infestation in Pomadasys stridense

Document Type : Original Article

Authors

10.21608/ejabf.2023.329194

Abstract

Fish in the Suez Canal region, a highly diverse ecosystem where many different species of fish interact, are at risk of mixed infections caused by multiple pathogens, including parasites and bacteria. This study highlighted the naturally occurring process of parasitic bacterial co-infections, specifically involving the parasite Livoneca redmanii and the bacterium Pseudomonas aeruginosa in the fish Pomadasys stridens. A total of 80 P. stridens fish were randomly collected from the Suez Canal coasts in Egypt over a period from September to November 2022. The fish samples were tested using standard parasitological and microbiological procedures to identify the presence of L. redmanii and P. aeruginosa. To definitively identify the parasite Livoneca redmanii, its mitochondrial Cytochrome Oxidase subunit I gene was sequenced, and the sequence was deposited in GenBank under the accession number OR051043. Furthermore, the presence of P. aeruginosa was isolated from the infested fish samples with isopods, and its identification was confirmed by molecular techniques. The prevalence of L. redmanii was 48.75%, while P. aeruginosa in the infested P. stridens fish with isopods was 69.23%. These findings shed light on the complex dynamics of parasitic bacterial co-infections among fish species in the region.

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