Role of Aqueous and Ethanolic Seed Extract of Asparagus racemosus on Acr-Induced Neurotoxicity in Adult Zebrafish: Emergence of Neuroprotective Results

The primary objective of this work was to examine the potential neuroprotective properties of a seed extract derived from Asparagus racemosus for neurotoxicity in an acrylamide-induced zebrafish model. After ACR treatment, fish went through neurotoxic effects where glutathione reductase levels decreased by 3 times; lipid peroxidation activity increased by 3.4 times; nitrite levels increased by 1.7 times; acetylcholinesterase levels increased by 3.9 times, and total protein levels decreased by 1.4 times, compared to the wild-type zebrafish. Treatment with the standard drug vinpocetine showed a significant level of neuroprotective activity in ACR-induced zebrafish, where glutathione reductase level was increased by 2.7 times; lipid peroxidation activity decreased by 3.4 times; nitrite level decreased by 1.5 times; acetylcholinesterase decreased by 3.2 times, and total protein increased by 1.4 times. The results were comparable to those of a wild type of zebrafish. Therapy using different solvent seed extracts of Asparagus racemosus after ACR exposure restored glutathione reductase, lipid peroxides, nitrite, protein, and acetylcholinesterase activity, which was comparable to control group levels. Among the two solvent extracts, the ethanolic extract showed much better results for neuroprotective activity in ACR-induced zebrafish. After treatment with 440mg/ l ethanolic seed extract, glutathione reductase level increased by 2.7 times; lipid peroxidation activity decreased by 3.1 times; nitrite level decreased by 1.4 times, and acetylcholinesterase decreased by 2.7 times. While total protein increased by 1.3 times in ACR-induced zebrafish. The results were comparable to those of the wild type of zebrafish.