Molecular identification of some zoonotic bacteria isolated from fishes Cyprinus carpio L. and Oreochromis niloticus (L.)

      Zoonotic bacteria isolated from fish cause many diseases, some of which are transmitted to humans, and it is a danger to public health in light of the recent spread of epidemics. The current study was designed to isolate and indentation bacterial species originating from two types of wild fishes (healthy and infected Cyprinus carpio and Oreochromis niloticus) in two different locations (Al-Mas`hab and Garmat Ali River) of water in Basrah, southern Iraq, during (2022) in two seasons of the year. The most prevalent species were identified in the two seasons (winter, and summer), the bacteria were isolated from the internal organs of fish (liver, kidneys, and spleen).  The morphological and biochemical tests were used to identify the bacteria. The most prevalent species in the two types of fishes were indentation by targeting a specific 16S rRNA gene of the bacteria. The bacterial species successfully identified in C. carpio were Aeromonas hydrophila, A. salmonicida, Vibrio parahaemolyticus, and Klebsiella oxytoca. Five bacterial species in fish O. niloticus were detected (A. bivalvium, V. parahaemolyticus, Pseudomonas sp., Citrobacter sp. and Shewanella algae). The study showed that the increase in environmental pollution factors the pathogenicity of the disease has been shown to vary seasonally in the internal organs of healthy and infected fish.