Malash et al., M. (2022). Antimicrobial Activities of a Novel Marine Streptomyces sp. MMM2 Isolated from El- Arish coast, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 26(5), 1317-1339. doi: 10.21608/ejabf.2022.268783
Mona A. Malash et al.. "Antimicrobial Activities of a Novel Marine Streptomyces sp. MMM2 Isolated from El- Arish coast, Egypt". Egyptian Journal of Aquatic Biology and Fisheries, 26, 5, 2022, 1317-1339. doi: 10.21608/ejabf.2022.268783
Malash et al., M. (2022). 'Antimicrobial Activities of a Novel Marine Streptomyces sp. MMM2 Isolated from El- Arish coast, Egypt', Egyptian Journal of Aquatic Biology and Fisheries, 26(5), pp. 1317-1339. doi: 10.21608/ejabf.2022.268783
Malash et al., M. Antimicrobial Activities of a Novel Marine Streptomyces sp. MMM2 Isolated from El- Arish coast, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 2022; 26(5): 1317-1339. doi: 10.21608/ejabf.2022.268783
Antimicrobial Activities of a Novel Marine Streptomyces sp. MMM2 Isolated from El- Arish coast, Egypt
There is diversity and novelty among actinomycetes present in marine environments. Marine microbial resources may lead to the discovery of a new antimicrobial agent added to the commercially used ones. In this study, several marine Actinomycetes were isolated from the Red Sea (Berenice and Safaga) and Mediterranean Sea (Arish and Abou Quir), and screened for their ability to produce antimicrobial agents (s) against Staphylococcus aureus, E.coli, Pseudomonas aeruginosa, Enterococcus faecalis, Bacillus subtilis, Klebsiella pneumoniae and Edwardsiella tarda using agar well diffusion. The most potent isolate identified as Streptomyces sp. MMM2 by the 16S rRNA sequence gene. Optimization of the antimicrobial products was performed by Plackett–Burman experimental design. Ethyl acetate, butanol, hexane, and acetone extracts were used for the screening. Anti-fungal activity of Streptomyces sp. MMM2 was also estimated. Chemical characterization of the ethyl acetate extract of Streptomyces sp. MMM2 was performed using GC-MS spectrophotometry, FTIR, Raman, and NMR. This analysis showed that effective compounds that had antimicrobial activities were hexadecanoic acid methyl ester, 9-octadecadienoic, octadecadienoic acid methyl ester, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, 9,12-octadecadienoyl chloride.
View on SCiNiTO
Top