• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Peer Review Process
  • Guide for Authors
  • Submit Manuscript
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Egyptian Journal of Aquatic Biology and Fisheries
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 29 (2025)
Volume Volume 28 (2024)
Volume Volume 27 (2023)
Volume Volume 26 (2022)
Issue Issue 6
Issue Issue 5
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Issue 1
Volume Volume 25 (2021)
Volume Volume 24 (2020)
Volume Volume 23 (2019)
Volume Volume 22 (2018)
Volume Volume 21 (2017)
Volume Volume 20 (2016)
Volume Volume 19 (2015)
Volume Volume 18 (2014)
Volume Volume 17 (2013)
Volume Volume 16 (2012)
Volume Volume 15 (2011)
Volume Volume 14 (2010)
Volume Volume 13 (2009)
Volume Volume 12 (2008)
Volume Volume 11 (2007)
Volume Volume 10 (2006)
Volume Volume 9 (2005)
Volume Volume 8 (2004)
Volume Volume 7 (2003)
Volume Volume 6 (2002)
Volume Volume 5 (2001)
Volume Volume 4 (2000)
Volume Volume 3 (1999)
Volume Volume 2 (1998)
Volume Volume 1 (1997)
H. Zaghloul, E., M. Hamed, M. (2022). Statistical optimization of fermentation conditions for bioactive compounds production by marine Acinetobacter sp. EM11 against the fish pathogen Vibrio alginolyticus MK170250. Egyptian Journal of Aquatic Biology and Fisheries, 26(1), 563-584. doi: 10.21608/ejabf.2022.221868
Eman H. Zaghloul; Moaz M. Hamed. "Statistical optimization of fermentation conditions for bioactive compounds production by marine Acinetobacter sp. EM11 against the fish pathogen Vibrio alginolyticus MK170250". Egyptian Journal of Aquatic Biology and Fisheries, 26, 1, 2022, 563-584. doi: 10.21608/ejabf.2022.221868
H. Zaghloul, E., M. Hamed, M. (2022). 'Statistical optimization of fermentation conditions for bioactive compounds production by marine Acinetobacter sp. EM11 against the fish pathogen Vibrio alginolyticus MK170250', Egyptian Journal of Aquatic Biology and Fisheries, 26(1), pp. 563-584. doi: 10.21608/ejabf.2022.221868
H. Zaghloul, E., M. Hamed, M. Statistical optimization of fermentation conditions for bioactive compounds production by marine Acinetobacter sp. EM11 against the fish pathogen Vibrio alginolyticus MK170250. Egyptian Journal of Aquatic Biology and Fisheries, 2022; 26(1): 563-584. doi: 10.21608/ejabf.2022.221868

Statistical optimization of fermentation conditions for bioactive compounds production by marine Acinetobacter sp. EM11 against the fish pathogen Vibrio alginolyticus MK170250

Article 35, Volume 26, Issue 1, January and February 2022, Page 563-584  XML PDF (1.16 MB)
Document Type: Original Article
DOI: 10.21608/ejabf.2022.221868
View on SCiNiTO View on SCiNiTO
Authors
Eman H. Zaghloul; Moaz M. Hamed
Abstract
Thirty-two marine bacterial strains were isolated from marine samples (seawater and sediments) collected from the Mediterranean Sea, Egypt. They were evaluated for their antimicrobial activity against the fish pathogen Vibrio alginolyticus MK170250, using the agar well-cut diffusion technique. The marine bacterial isolates EM11 showed significant activity against V. alginolyticus MK170250 with an inhibition zone of (10 mm). The isolate EM11 was phenotypically characterized and molecularly identified through 16S rRNA gene sequence analysis as Acinetobacter indicus. Placket-Burman and Box-Behnken experimental designs were applied to optimize the fermentation conditions and increase the bioactive compounds' productivity. The optimized medium was formulated as follows: (gl-1) Peptone 7 g; beef extract: 2 g; inoculum’s size (ml) 0.5; sea water concentration: 75%; pH: 6.02; temperature (°C) 40 for 24h incubation period. A verification experiment was applied to evaluate the basal versus the optimized medium. The optimized growth condition successfully increased the inhibition zone about 3.65-fold with an inhibition zone of 36.5 mm in diameter. To the best of our knowledge, this is the first report of Acinetobacter indicus species from marine sediments and its anti-fish pathogen activity.
Keywords
Antimicrobial; Marine Acinetobacter indicus; Vibrio alginolyticus; Optimization; Placket-Burman and Box-Behnken design
Statistics
Article View: 342
PDF Download: 513
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by NotionWave.