Cytotoxicity and antimicrobial activities of the novel lactic acid bacterium, Lactobacillus brevis A13, isolated from the gut of the polychaete, Perinereis nuntia

M. Hamdan Et Al., Amira

doi:10.21608/ejabf.2022.221762

Cytotoxicity and antimicrobial activities of the novel lactic acid bacterium, Lactobacillus brevis A13, isolated from the gut of the polychaete, Perinereis nuntia

Document Type : Original Article

Author

Amira M. Hamdan et al.

10.21608/ejabf.2022.221762

Abstract

The present study was implemented to evaluate the cytotoxicity and antimicrobial activities of the novel lactic acid bacterium, Lactobacillus brevis A13, isolated from the gut of the polychaete worm, Perinereis nuntia. The results indicated that L. brevis A13 acquired a broad antimicrobial spectrum against the tested bacterial pathogens with multiple antibiotic resistance (MAR) index value of 0.57. Pure bacteriocin had a molecular weight of approximately 4.0 kDa and was designated as "Brevicin-13". Furthermore, brevicin-13 had a bactericidal nature, causing disruption and deformation of the cell membrane with cavities observed on the surface of Klebsiella pneumoniae ATCC 13883 cells. Compared to untreated cancer cells, brevicin-13 exhibited very strong cytotoxic activity, with inhibition rates of 93%, 95%, and 91% against three cancer cell lines; colon cancer (Caco-2), liver cancer (HepG2), and breast cancer (Mcf-7), respectively. The inhibition was clearly shown as anti-proliferative activity and detrimental effects of the cell nucleus due to the induction of apoptosis upon exposure to brevicin-13.

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