EXPRESSION OF THE INTERFERON RESPONSIVE GENE IN THE COMMON CARP (CYPRINUS CARPIO)

Khafagy, Omaima; Zelenin, Inessa

doi:10.21608/ejabf.2006.1909

EXPRESSION OF THE INTERFERON RESPONSIVE GENE IN THE COMMON CARP (CYPRINUS CARPIO)

Document Type : Original Article

Authors

Omaima Khafagy ¹
Inessa Zelenin ²

¹ Department of fish Resources and Aquaculture, Faculty of Environmental Agricultural Sciences, Suez Canal University, El-Arish, Egypt

² Engelhardt Institute of Molecular Biology, Moscow Academy of Sciences, Russia.

10.21608/ejabf.2006.1909

Abstract

Viral haemorrhagic septicaemia (VHS) is an important rhabdo viral disease in the fish farming . Over recent years a highly effective experimental DNA vaccine to VHS has been developed. Virus-induced genes were ultimately retrieved from the subtracted cDNA library, and their differential expression was further confirmed by semi quantitative reverse transcription-PCR and Northern blot analysis. The ability of the plasmid to mediate expression of the viral G-protein on the surface of transfected cells was confirmed by specific immunostaining for the viral protein. The type 1 interferon (IFN) system is a rapid and powerful antiviral defense. Using a luciferase cell line to detect expression of IFN, it was shown that the Common Carp (Cyprinus carpio) Gonad cell line (CCG - p1), produces IFN when transfected with a plasmid encoding the glycoprotein of VHSV, but not with plasmid vector alone.
The results showed that the cells transfected with pcDNA3 vhs-G plasmid were expressing G protein, while the cells transfected with pcDNA3 plasmid did not show any staining. The cells transfected with the pcDNA3 vhs-G showed an increased luciferase activity of 5.4-fold compared with the control non-transfected cells . this increase was statistically significant (p < 0.05).

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