T. Sabeeh, H., J. J. Alshami, I., A. Al-Tameemi, R. (2021). Histochemical study of the intestine in the common carp Cyprinus carpio fingerlings to detect the impact of the replacement of dietary fish and soybean meals with shrimp wastes. Egyptian Journal of Aquatic Biology and Fisheries, 25(2), 721-734. doi: 10.21608/ejabf.2021.169190
Huda T. Sabeeh; Ilham J. J. Alshami; Riyadh A. Al-Tameemi. "Histochemical study of the intestine in the common carp Cyprinus carpio fingerlings to detect the impact of the replacement of dietary fish and soybean meals with shrimp wastes". Egyptian Journal of Aquatic Biology and Fisheries, 25, 2, 2021, 721-734. doi: 10.21608/ejabf.2021.169190
T. Sabeeh, H., J. J. Alshami, I., A. Al-Tameemi, R. (2021). 'Histochemical study of the intestine in the common carp Cyprinus carpio fingerlings to detect the impact of the replacement of dietary fish and soybean meals with shrimp wastes', Egyptian Journal of Aquatic Biology and Fisheries, 25(2), pp. 721-734. doi: 10.21608/ejabf.2021.169190
T. Sabeeh, H., J. J. Alshami, I., A. Al-Tameemi, R. Histochemical study of the intestine in the common carp Cyprinus carpio fingerlings to detect the impact of the replacement of dietary fish and soybean meals with shrimp wastes. Egyptian Journal of Aquatic Biology and Fisheries, 2021; 25(2): 721-734. doi: 10.21608/ejabf.2021.169190
Histochemical study of the intestine in the common carp Cyprinus carpio fingerlings to detect the impact of the replacement of dietary fish and soybean meals with shrimp wastes
The present study investigated the effect of replacement of fish meal and soybean meal with shrimp wastes meal on the intestine tissue in common carp Cyprinus carpio fingerlings. Five isonitrogenous (31.66±0.40) and isocaloric (405.57±5.35 kg / 100 g) diets were formulated including control diet (C), 25% and 50% of the total dietary protein replacement of fish meal (T1 and T2 respectively) or soybean meal (T3 and T4 respectively) with shrimp waste meal. Fish were fed with a formulated diet for 63 days. The histochemical study showed increases in the length of villi and the number of goblet cells, and appearance necrosis in intestine layers, degeneration, shrinking of supranuclear vacuoles, detachment of base of villi, and infiltration of inflammatory eosinophilic granulocytes. Histochemical examination using alcian blue and periodic acid–Schiff revealed three types of specific goblet cells in the middle intestine which secrete neutral mucus, acidic mucus, and a mixture of acidic and neutral mucus depending on their functions. This study argued at the first time effect of formulated diet on intestine tissue histochemically.