Insecticidal Activity and Biochemical Study of the Clove Oil (Syzygium aromaticum) Nano- Formulation on Culex pipiens L. (Diptera: Culicidae)

E. El Gohary, El Gohary; M. Farag, Shaimaa; A. El-Sayed, Ahmed; R. Khattab, Reham; M. Mahmoud, Dalia

doi:10.21608/ejabf.2021.137233

Insecticidal Activity and Biochemical Study of the Clove Oil (Syzygium aromaticum) Nano- Formulation on Culex pipiens L. (Diptera: Culicidae)

Document Type : Original Article

Authors

10.21608/ejabf.2021.137233

Abstract

In the present study Syzygium aromaticum essential oil (EO) was extracted using water as a solvent and encapsulated within chitosan nanoparticles, which are characterized by Ultraviolet-Visible (UV-VIS) spectra, Infra-Red (IR), Transmission Electron Microscopy (TEM), and X-ray diffraction (XRD). The insecticidal activity for both Syzygium aromaticum bulkEO and encapsulated EO within chitosan nanoparticles was determined. Both of the two oil forms were assessed as a larvicide against third instar larvae of Culex pipiens by measuring LC values. Encapsulated nano-formulation EO showed higher toxicity (LC₅₀=20 ppm) than the bulk EO (LC₅₀=39 ppm). The biochemical changes were measured after treatment with both forms of EO. There is an increase in the activity of three enzymes (acid phosphatase, alkaline phosphatase, and glutathione-S-transferase), while the activity of acetylcholinesterase was decreased. The obtained results revealed that water extract of Syzygium aromaticum EO and nano-encapsulated EO may be promising alternative larvicide for controlling Culex pipiens larvae in integrated pest management.

Keywords