E. El Gohary, E., M. Farag, S., A. El-Sayed, A., R. Khattab, R., M. Mahmoud, D. (2021). Insecticidal Activity and Biochemical Study of the Clove Oil (Syzygium aromaticum) Nano- Formulation on Culex pipiens L. (Diptera: Culicidae). Egyptian Journal of Aquatic Biology and Fisheries, 25(1), 227-239. doi: 10.21608/ejabf.2021.137233
El Gohary E. El Gohary; Shaimaa M. Farag; Ahmed A. El-Sayed; Reham R. Khattab; Dalia M. Mahmoud. "Insecticidal Activity and Biochemical Study of the Clove Oil (Syzygium aromaticum) Nano- Formulation on Culex pipiens L. (Diptera: Culicidae)". Egyptian Journal of Aquatic Biology and Fisheries, 25, 1, 2021, 227-239. doi: 10.21608/ejabf.2021.137233
E. El Gohary, E., M. Farag, S., A. El-Sayed, A., R. Khattab, R., M. Mahmoud, D. (2021). 'Insecticidal Activity and Biochemical Study of the Clove Oil (Syzygium aromaticum) Nano- Formulation on Culex pipiens L. (Diptera: Culicidae)', Egyptian Journal of Aquatic Biology and Fisheries, 25(1), pp. 227-239. doi: 10.21608/ejabf.2021.137233
E. El Gohary, E., M. Farag, S., A. El-Sayed, A., R. Khattab, R., M. Mahmoud, D. Insecticidal Activity and Biochemical Study of the Clove Oil (Syzygium aromaticum) Nano- Formulation on Culex pipiens L. (Diptera: Culicidae). Egyptian Journal of Aquatic Biology and Fisheries, 2021; 25(1): 227-239. doi: 10.21608/ejabf.2021.137233
Insecticidal Activity and Biochemical Study of the Clove Oil (Syzygium aromaticum) Nano- Formulation on Culex pipiens L. (Diptera: Culicidae)
In the present study Syzygium aromaticum essential oil (EO) was extracted using water as a solvent and encapsulated within chitosan nanoparticles, which are characterized by Ultraviolet-Visible (UV-VIS) spectra, Infra-Red (IR), Transmission Electron Microscopy (TEM), and X-ray diffraction (XRD). The insecticidal activity for both Syzygium aromaticum bulkEO and encapsulated EO within chitosan nanoparticles was determined. Both of the two oil forms were assessed as a larvicide against third instar larvae of Culex pipiens by measuring LC values. Encapsulated nano-formulation EO showed higher toxicity (LC50 =20 ppm) than the bulk EO (LC50 =39 ppm). The biochemical changes were measured after treatment with both forms of EO. There is an increase in the activity of three enzymes (acid phosphatase, alkaline phosphatase, and glutathione-S-transferase), while the activity of acetylcholinesterase was decreased. The obtained results revealed that water extract of Syzygium aromaticum EO and nano-encapsulated EO may be promising alternative larvicide for controlling Culex pipiens larvae in integrated pest management.
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