Histological alterations in some organs of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) produced using methyltestosterone

Document Type : Original Article

Authors

1 Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt

2 Faculty of science, Al-Azhar University (Assiut), Egypt

3 National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt

Abstract

17α-methyltestosterone is the most efficient and least expensive method in monosex production in Egypt. This study was carried out to investigate the effect of this hormone on farmed Nile tilapia (Oreochromis niloticus) using some histological parameters. Fish samples were collected from four localities (Assiut; as a control and Beheira, Alexandria, Kafr El-Sheikh; as monosex populations). Histological sections in liver, kidney, and testis were prepared, stained, and examined according to histology protocol. Microscopic examination has been done for random samples showing some alterations, such as damages central vein (CV), nicrotic area (N), and hyperplasia in the hepatocytes (H) and Kuffer cells (Kc) in fish collected from Kafr El-Shiek farms. Sections of liver of tilapia from Alexandria showed hydropic degeneration (HD) in the hepatocytes (H), hyperplasia in the Kupffer cells (Kc) and blood congestion (BC) in the central vein.  Moreover, sections of liver of tilapia from Beheira showed the hepatocytes (H) and the central vein (CV) with presence of melanomacrophages (M) and hyperplasia in the hepatocytes and Kupffer cells (Kc).  Kidney of tilapia from monosex farms showed enlarged glomeruli (EG) and damaged renal tubules, detached epithelial cells and damaged renal tubules. Testis showed degeneration of the interstitial tissues and disorganization in the seminiferous tubules. It has been concluded that, the overdose of methyltestosterone (MT) induction affected some oranges in monosex fish.

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