Amelioration of anxiety and locomotion during circadian rhythm change of adult zebrafish (Danio rerio) by Pranic Energy

Document Type : Original Article

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Abstract

Abnormal stress level, anxiety, and reduced locomotion are prominent symptoms after circadian rhythm is changed. This study investigates the influence of pranic energy on zebrafish behaviour during circadian rhythm changes. Adult zebrafish were kept in control and experimental aquariums. As an adjunct, the experimental fishes (Pranic group) received the Pranic Healing techniques for fish, food, and water to reinforce the energy in them.  Individual fishes were exposed in an open area for 5 minutes and parameters including average speed, mobile speed, average acceleration, mobility rate, exploratory rate, total distance travelled, and total immobile were measured and analysed by Tox-Track tracking software. Stress was created to fishes by alternating circadian rhythm for 3 days for both groups. The changes in locomotion and anxiety data were analysed using One Way ANOVA and Mann-Whitney U test with SPSS software. Pranic treated zebrafishes (46) had increased average speed of 8.18 mm/s (p < .002), mobile speed of 7.56 mm/s (p < .000), average acceleration of 323.71 mm/s2 (p < .000), mobility rate of 11.36% (p < .009), total distance travelled of 29.490m (p < .000)and total immobile of 30.68s (p < .016) compared to control group (43). Pranic energy positively influenced the behaviour of adult zebrafish by increased locomotion and decreased anxiety levels during changed circadian rhythm

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Egyptian Journal of Aquatic Biology and Fisheries
Volume 24, Issue 6 - Serial Number 6
September and October 2020
Pages 319-329

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