S. Yaseen, M., Abdelaziz, M., A. Abdel-moneam, D., Abd-Elhay, E., M. Wassif, I., fawzy, M., Moustafa, M. (2020). Phenotypic and Genotypic characterization of the pathogenic Pseudomonas aeruginosa isolated from cultured Pangasianodon hypophthalmus in Egypt.. Egyptian Journal of Aquatic Biology and Fisheries, 24(6), 453-467. doi: 10.21608/ejabf.2020.117086
Mai S. Yaseen; Mohamed Abdelaziz; Dalia A. Abdel-moneam; Elsayed Abd-Elhay; Islam M. Wassif; Marwa fawzy; Mohamed Moustafa. "Phenotypic and Genotypic characterization of the pathogenic Pseudomonas aeruginosa isolated from cultured Pangasianodon hypophthalmus in Egypt.". Egyptian Journal of Aquatic Biology and Fisheries, 24, 6, 2020, 453-467. doi: 10.21608/ejabf.2020.117086
S. Yaseen, M., Abdelaziz, M., A. Abdel-moneam, D., Abd-Elhay, E., M. Wassif, I., fawzy, M., Moustafa, M. (2020). 'Phenotypic and Genotypic characterization of the pathogenic Pseudomonas aeruginosa isolated from cultured Pangasianodon hypophthalmus in Egypt.', Egyptian Journal of Aquatic Biology and Fisheries, 24(6), pp. 453-467. doi: 10.21608/ejabf.2020.117086
S. Yaseen, M., Abdelaziz, M., A. Abdel-moneam, D., Abd-Elhay, E., M. Wassif, I., fawzy, M., Moustafa, M. Phenotypic and Genotypic characterization of the pathogenic Pseudomonas aeruginosa isolated from cultured Pangasianodon hypophthalmus in Egypt.. Egyptian Journal of Aquatic Biology and Fisheries, 2020; 24(6): 453-467. doi: 10.21608/ejabf.2020.117086
Phenotypic and Genotypic characterization of the pathogenic Pseudomonas aeruginosa isolated from cultured Pangasianodon hypophthalmus in Egypt.
This study was conducted on 70 diseased Pangasius hypophthalmus fish collected from different Pangasius fish farms in Egypt. Bacteriological examination revealed the isolation of P. aeruginosa that was biochemically confirmed using MALDI-TOF MS and VITEK 2 compact system. The prevalence and virulence of Pseudomonasaeruginosa were estimated genotypically using species-specific 16S rDNA gene, OprL and ToxA virulent genes for detection of pathogenic strains followed by sequencing analysis. Pathogenicity of isolated Pseudomonas aeruginosa was confirmed by experimental intraperitoneal injection of healthy Pangasius hypophthalmus with positively virulent strains resulting in high mortalities among injected fish.
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