The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613121220170401Inter Simple Sequence Repeat (ISSR) and Cytogenetic Analysis of Three Fish Species of Family Osphronemidae115243610.21608/ejabf.2017.2436ENAli, H. Abu-AlmaatyZoology Department, Faculty of Science, Port Said UniversityMohamed, K. HassanZoology Department, Faculty of Science, Port Said University.Iman, M. BahgatZoology Department, Faculty of Science, Port Said University.Mariam, E. E. SuleimanZoology Department, Faculty of Science, Port Said University.Journal Article20170330The ISSR-PCR and karyological analysis methods are successfully used to detect the genetic variations between the different species of fishes, especially these belonging to the same family. The aim of this study was to determine the karyotypes and molecular genetic variations of three species of family Osphronemidae, <em>Trichogaster trichopterus, Trichogaster leeri</em> and<em> Colisa lalia</em>by using ISSR-PCR and chromosomal analysis. Samples were collected from ornamental fish farms in Egypt. The diploid chromosome numbers and Fundamental numbers for species under study were 2n = 46 and FN = 46, 2n = 46 and FN = 46, 2n = 46 and FN = 70 respectively, the first two species have same karyotype but the third species was different. The ISSR-PCR analysis was carried out using ten primers. All primers were successfully amplified on the genomic DNA extracted from all studied fish species. These findings indicate that ISSR-PCR and cytogenetic analysis are very useful in determination of genetic molecular variations and relationship degree between the species which belong to same family.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613121220170401Impact of Drainage Water on Macrobenthos Structure of Lake Qaroun, El-Fayoum, Egypt1732328910.21608/ejabf.2017.3289ENMagdy T. KhalilFaculty of Science, Ain Shams University, Cairo, Egypt.Mohamed R. FisharNational Institute of Oceanography and Fisheries, Inland Water Branch, Egypt.Safwat H ShakirTexas A&M University System, Texas, U.S.A.Amany, S. AmerCentral Lab. For Environmental Quality Monitoring, National Water Research Center, Egypt.Marian G. NassifNational Institute of Oceanography and Fisheries, Inland Water Branch, Egypt.Journal Article20170328Lake Qaroun is used as a reservoir for agricultural drainage water via El-Bats and El-Wadi drains. Some physico-chemical parameters and macrobenthic invertebrate community structure were seasonally studied at different stations in the lake during 2014 to 2015. The results revealed that the stations in front of El-Bats and El-Wadi drains suffer from low transparency and decrease of its oxygen content. Moreover, the station in front of El-Bats drain recorded the highest values of the biological oxygen demand (BOD) and phosphates as a result of high amount of discharged organic matter. In addition, the station in front El-Wadi drain revealed the highest concentrations of nitrate, copper and iron. Although the structure of macrobenthos assemblage seems to be complex and influenced by many factors, it was remarkable that the station in front El-Wadi drain had an adverse effect on it. Consequently, continuous and increased pollution by the drainage water contaminated with sewage and heavy metals affected severely most of Lake Qaroun organisms. Therefore, sewage and drainage waste water should be treated before discharging into the lake.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613121220170401Length-Weight Relationship and Condition Factor of Some Fishes from the River Nile in Egypt with Special Reference to Four Tilapia Species3346329610.21608/ejabf.2017.3296ENKariman A. Sh. ShalloofBiology Department, Faculty of Science and Home Domestics, Bisha University, Saudi Arabia and Fisheries Division, National Institute of Oceanography and Fisheries, Egypt.Alaa M. El- FarFisheries Division, National Institute of Oceanography and Fisheries, Egypt.Journal Article20170331This study has been carried out along the River Nile (river main stream, Rosetta and Damietta branches) in Egypt. Fish Samples of 21 species belonging to 11 families were collected mainly from the catch of three fishing gears and methods,Trammel net, Traps, and Long-lines. Total length (cm) and body weight (g) were measured and data were applied to the equation of W= a L<strong><sup>b</sup></strong>, for the length-weight relationship and K= 100 × W / L<sup>3 </sup>for condition factor. Four cichlid species, namely: <em>Oreochromis niloticus, Oreochromis aureus, Tilapia zillii and Sarotherodon galilaeus </em>were investigated in details. <em>T. zillii</em> exhibits isometric growth in both main stream and Damietta branch, where, relative growth coefficient (b) = 3.0266 and 2.9698, respectively. <em>S. galilaeus</em> follows the same pattern in Rosetta branch (b = 3.0154). Generally, allometric growth noticed for the four studied species in the three branches of the River Nile. The value of (K) of different cichlid species was fluctuated during different seasons in the three studied areas of the River Nile. The value of condition factor of the four studied species inversely related to the size of fish. In general, variation in the most studied biological characters of cichlid species in the three studied areas of River Nile was noticed. Allometric growth for the four studied species in the three branches of the river indicating increase of pollution. The present study recommends that these selected sectors need proper management.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613121220170401Pathogenicity of Fungi Colonizing Some Hard Corals and Invertebrates from the Northern Egyptian Red Sea Coast4761353210.21608/ejabf.2017.3532ENEl-Morsy, E. M.Botany and Microbiology Department, Faculty of Science, Damietta University, EgyptIbrahim, H. A. HNatinoal Institute of Oceanography and Fisheries, Alexandria Branch, Alexandria, EgyptAmal ZakiFarhatBotany and Microbiology Department, Faculty of Science, Damietta University, EgyptMohsien, M. T.Botany and Microbiology Department, Faculty of Science, Damietta University, EgyptAbu El-Regal, MMarine Science Department, Faculty of Science, Port Said University, Port said, EgyptJournal Article20170301Fungi colonizing hard coral species collected from Hurghada, Red Sea were isolated and identified to the species<br />level. A total of 47 fungal isolates (37 isolates from hard corals and 10 isolates from other invertebrates) were collected.<br />Twelve of them are belonging to 4 genera; Aspergillus, Penicillium, Nigrospora and Botrydiploida. Aspergillus and<br />Penicillium were represented by 5 species each whereas, Nigrospora and Botrydiploida were represented by one species<br />each. Pathogenicity of 12 fungal species on Galaxea fascicularis and Stylophora pistillata corals revealed that that<br />the degree of pathogenicity depend up on the fungal species, hard coral species and the duration of exposure to spores.<br />Colonies of G. fascicularis were more susceptible to fungal infection than S. pistillata where they were infected in the first<br />week by spores of four fungi compared to one fungal species for S. pistillata. G. Fascicularis was infected by Aspergillus<br />niger, and A. parasiticus where black spots appeared on some parts of the colonies and the skeleton started to decay. In<br />general A. flavus, A. fumigatus, Botryoldiploida sp., P. crustosum, and P. echinulatum had no effect on coral colonies.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613121220170401A first Record of Aulopareia unicolor (Valenciennes, 1837) (Family: Gobiidae) in the Mediterranean Sea, Egypt.6366353310.21608/ejabf.2017.3533ENElsayed Haroun AkelKait-Bey, Alexandria, Egypt.0000-0003-3072-0300Samir, I.RizkallaFishery Biology Lab, National Institute of Oceanography and Fisheries, Kait-Bey, Alexandria, Egypt.Journal Article20170301The Gobiid species Aulopareia unicolor (Valenciennes, 1837) (Family: Gobiidae) has not been known from the Mediterranean waters. Our presently recorded findings (two specimens from Port Said and six from Damietta) extend the geographical distribution of this species to the Mediterranean Sea. The morphometric analyses of this species were recorded in the Egyptian Mediterranean waters.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613121220170401Food and Feeding Habits of Two-Bar Seabream, Acanthopagrus bifasciatus (Forsskål, 1775) from Southern Red Sea, Egypt6778353410.21608/ejabf.2017.3534ENSahar, F.MehannaFisheries Division, National Institute of Oceanography and FisheriesFahmy, I.El-GammalFisheries Division, National Institute of Oceanography and FisheriesUsama, M.MahmoudZoology Department, Faculty of Science, Assiut UniversitySamia MohsenEl-MahdyFish population dynamic lab, Fisheries division, National Institute of Oceanography and Fisheries, Hurghada0000-0002-0724-7001Journal Article20170401The food and feeding habits of the two-bar seabream, Acanthopagrus bifasciatus fishes from Southern Red Sea, Egypt<br />were studied to know its feeding behavior in relation to sex, length and season as well as its preference for food items. Fish samples were collected monthly covering a period of 12 months from January 2015 to December 2015 and a total of 227 fish stomachs were examined for this study. The results indicated that Acanthopagrus bifasciatus fish feed on a variety of food materials mainly Mollusks (gastropods, bivalves and chitons), crustaceans (hermit crabs, crabs and eggs of hermit crab), fish scales, cnidarians (soft coral), Echinodermata as well as food remains. The variety of food substances in the stomach of Acanthopagrus bifasciatus shows that the species is a carnivorous. The feeding intensity showed its highest value during spring (76.74%) followed by winter (73.58%), while the lowest value was recorded during summer (57.14%). Acanthopagrus bifasciatus shifted their fullness index as they grow in length where the lowest value of<br />this index was recorded for a length group 17–25 cm TL and the highest value was recorded for a length group 36–45 cm TL. The feeding habits of Acanthopagrus bifasciatus showed no significant difference between males and females and both sexes have the same diet composition with nearly the same concentration and distribution except for bivalves which were recorded only in the stomachs of female.