The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613119320150901First record of the cuttlefish Sepia dollfusi (Cephalopoda: Sepioidea) from the Egyptian Mediterranean waters17226610.21608/ejabf.2015.2266ENRafik RiadNational institute of Oceanography and Fisheries, Alexandria, EgyptJournal Article20170405In the present work, one specimen of female Sepia dollfusi; 12.2 cm mantle length (ML) and 250 gm total weight (To. Wt.) was randomly sampled from commercial fish trawling net during December 2014 in the area off the Alexandria. It<br />was belonging to Family Sepiidae. This species is dwelling the Egyptian Red Sea and Sues Gulf. Worth mentioning, this is the first record of this species from the Egyptian Mediterranean waters. Taxonomical and describing detailed study of this species has been done.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613119320150901Genetic relationship between five fish species of genus scarus using rapd assay922226710.21608/ejabf.2015.2267ENAli AlmaatyZoology Department, Faculty of Science, Port Said UniversityMohamed El_RegalMarine Science Department, Faculty of Science, Port Said University, EgyptZeinab Mar`ieCytogenetic Laboratory-Zoology Department- Faculty of Science (Qena) -Basset EbiedCytogenetic Laboratory-Zoology Department- Faculty of Science (Qena) -Journal Article20170405Scaridae fish species including Scarus psittacus, S. frenatus, S. genazonatus, S. fuscopurpureus and S. niger, were collected from Red Sea in Egypt. The DNA fingerprints of these species were identified using RAPD-PCR technique with eight decamer primers. A total of 93 bands were scored in all species using the selected primers, with the size range varying from 190 to 2500 bp. On average, each primer amplified 11.63 bands per species. Each species had both monomorphic and polymorphic DNA bands. The polymorphic bands represent 34.41% in all fish species. The highest similarity index (70%) was found between Scarus psittacus and S. frenatus, while the lowest one (51%) was between Scarus psittacus and S. niger, S. genazonatus and S. fuscopurpureus and S. genazonatus and S. niger. Therefore the<br />phylogenetic relationships among the five studied scaridae species were clearly resolved in dendrogram, they were clustered into four clusters groups: the first one comprised, S. fuscopurpureus and S. niger, the second included S. psittacus, S.<br />frenatus and S. genazonatus, the third included S. genazonatus and the fourth branch<br />contained S. psittacus and S. frenatus. In conclusion, the obtained results exhibited<br />that the five Scarus species are distinctive ones. In addation, the RAPD patterns are<br />suitable tools to differentiate between fish species.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613119320150901Using distillers dried grains as an alternative protein source in Nile tilapia (Oreochromis niloticus) feeds2333226810.21608/ejabf.2015.2268ENMagdy SoltanDepartment of Animal production, Faculty of Agriculture, Banha University, EgyptAhamed RadwanDepartment of Animal production, Faculty of Agriculture, Banha University, EgyptAshraf GomaaRegional Laboratory for Food and Feed, Agriculture Research Center, Giza., Egypt.Journal Article20170405This work was conducted in the regional laboratory for food and feed,<br />Agriculture Research Center, Giza, Egypt, in a closed recirculation water system for<br />12 weeks to determine the effect of replacing 0, 10, 20, 30 and 40% soybean meal and<br />yellow corn in tilapia (Oreochromis niloticus) diets by Distillers Dried Grains<br />(DDGS) with and without enzyme (Ameco Zyme 2x, 0.25g/kg). Therefore, nine<br />isonitrogenous (30% CP) and isocaloric (3200 kcal GE/kg) experimental diets were<br />formulated, D1, repsant the (control). In the other experimental diets, soybean meal<br />and yellow corn mixture was replaced with DDGS at a rate of 10% (D2), 20% (D3),<br />30% (D4), 40% (D5), 10% DDGS+ Enzyme (D6), 20% DDGS+Enzyme (D7), 30%<br />DDGS+ Enzyme (D8) and 40% DDGS+ Enzyme (D9). A total number of 405 tilapia<br />fry (0.98 g±0.01) were randomly distributed into 27 tanks (60 liters each) at a<br />stocking rate of 15 fry/tank. After 84 days from the experiment start no significant<br />differences (P<0.05) were observed in survival rate, the highest specific growth rate<br />(3.5 %/day) was recorded for fish feed D7, while fish fed D5 showed the lowest value<br />(2.83 %/ day). The best feed conversion ratio (FCR) was occurred when fish fed D6<br />or D7 while the worst value was recorded by fish group fed D5. Protein efficiency<br />ratios (PER) were found to be high when fish were fed diets containing enzyme. Body<br />crude protein content for fish fed D1, D2, D6 or D7 were significantly higher than the<br />other diets while fish group fed D5 showed the highest significant (P<0.05) body fat<br />content compared to fish fed the other diets. The obtained results refers to the<br />possibilities of replacing soybean meal and yellow corn mixture in the control diets<br />with DDGS up to 20% without enzyme and up to 30% with enzyme addition.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613119320150901Assessment of water quality of unfertilized fish farm using zooplankton diversity index and some abiotic factors3544226910.21608/ejabf.2015.2269ENAhmed HeneashNational Institute of Oceanography and Fisheries (NIOF), Alexandria, EgyptJournal Article20170405The goal of this study was to investigate the effects of Nile tilapia and striped mullet fry stocking on the water quality of unfertilized fish farm using water quality index (WQI) method and zooplankton diversity index. Samplings were undertaken from pond 12 from one sample point over a 20- day period. The results indicate that, fry stocking showed detectable changes in the zooplankton assemblages. However, small differences in some limnological variables were noticed. Soon after the fry stocking, rotifers which constituted more than 90% of total zooplankton community<br />were replaced by Copepoda and Cladocera. To qualify the fish farm water, two indices were applied. Water quality (WQI) and zooplankton diversity indices were the same in appreciation. Diversity Index classified the pond water as being among moderately and heavily polluted, whereas the WQI demonstrated it as among medium and bad. It is suggested that Observation of the fish farm is necessary for proper Administration. Application of the WQI is also suggested as a very useful tool that enables the public and decision-makers to estimate water quality of fish farms.The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613119320150901A comparison on the commercial quality of some seafood on the basis of their percent edibility4553227010.21608/ejabf.2015.2270ENSamya MohammadDepartment of Zoology, Faculty of Science, Port Said University, EgyptJournal Article20170405The present study aimed to investigate the meat content and edibility in the commercial cockles (Cerastoderma glaucum) and gastropods (Thais carinifera) in relation to their sizes and weights. These species are among the most exploitable<br />seafood around the world and locally. They represent a considerable economic importance, both in terms of aquaculture and harvested aquatic resource. The present results showed a significant variation in the edibility with seasons. This variation did not correlate to the spawning activities. A positive correlation in the percentage<br />edibility with shell dimensions was recorded in the case of C. glaucum. On the other hand, a negative correlation with shell dimensions was obvious in T. carinifera. Additionally, both species exhibited negative correlations with weights. So, it can be proved that the quality of meat was in the optimum in the largest cockle and in the moderate size of the gastropod and the meat content being dependent upon the shell dimension rather than the shell weight. Consumers can obtain this sea food with good qualities most of the year except in summer (for C. glaucum) and winter (for T.<br />carinifera). Finally, the present study put a preliminary imagination to consumers forgetting their needs from seafood with good meat content at least cost. The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613119320150901Role of probiotics in improving growth performance, immunity and controlling Aeromonas hydrophila in the Nile tilapia Oreochromis niloticus5570227110.21608/ejabf.2015.2271ENMohamed MehisanRegional Center for Food & Feed (RCFF), Agriculture Research Center, EgyptGihan El-moghazyRegional Center for Food & Feed (RCFF), Agriculture Research Center, EgyptAbd El-Karim El-Sayed2Faculty of Agriculture-Benha University, EgyptMahmoud IraqiFaculty of Agriculture-Benha University, EgyptMagdy SoltanFaculty of Agriculture-Benha University, Egypt.Journal Article20170405The tilapias were treated with of Enterococcus faecium and Bacillus coagulans at a final concentration of (107cfu/g) (107cfu/g). 18 aquaria with 6 replicates for treated and controls were used. After 75 days, the tilapias supplemented with the probiotic showed significantly better final weight, body length, specific growth rate, weight gain, feed intake, feed conversion ratio and protein efficiency ratio than those fed on the basal diet (Control). After 75 days from experiment start, each experimental fish group was divided into two groups; the first group was infected with Aeromonas hydrophila while the second group without infection. The best Hb, Htc, MCV and RBCs content was recorded by fish group fed on the E. faecium supplemented diet comparing to fish group fed on the basil diet and infected with A. hydrophila and the same trend was observed for mortality rate. The highest WBCs value and differential count were recorded fish groups infected with A. hydrophila. Results also showed that, fish fed on the diet supplemented with E. faecium followed<br />by Bacillus coagulans recorded the lowest (P< 0.001) AST value (16.3 u/L) and (17.23 u/L), while control positive Aeromonas hydrophila group showed the highest (P>0.001) values being 29.6 u/L.