The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101GC/MS identification and applications of bioactive seaweed extracts from Mediterranean coast of Egypt1211795210.21608/ejabf.2018.17952ENSahar W. M. HassanMarine Microbiology Lab, Division of Marine Environment, National Institute of Oceanography and Fisheries, EgyptAida H. ShobierMarine Pollution Lab, Division of Marine Environment, National Institute of Oceanography and Fisheries, EgyptJournal Article20180921The antibacterial activity of different seaweed species against five fish<br />pathogens (A. hydrophila, V. parahaemolyticus, V. alginolyticus, V. damsela and<br />Vibrio sp.) has been evaluated. The ethanolic extract of C. sinuosa (CSE1) and<br />dichloromethane extract of C. officinalis (COM) exhibited the highest antibacterial<br />activity with activity index (AI) = 1.4 ±0.74 and 1.2 ±0.71, respectively. When the<br />extracts were encapsulated into calcium alginate beads their antibacterial activity<br />against the most susceptible bacterial pathogens showed that the significant growth<br />inhibition was against V. parahaemolyticus after 5 min post addition of the beads<br />recording 1.4 and 2 fold decrease in the growth, respectively. Recycling of the<br />(CA/COM) beads was carried out for 7 successive cycles with success in<br />elimination of V. parahaemolyticus. The potential applications of encapsulated<br />extracts for elimination of microbial load in fish rearing water and antifouling<br />activity were proved in the current investigation. The chemical composition of the<br />bioactive extracts (CSE1) and (COM) was determined using GC/MS analysis<br />which revealed the presence of several constituents that have been reported to<br />exhibit antimicrobial activity. Additionally, the dichloromethane extract (COM)<br />produced six major components including n-nonadecane, 1,2,3-<br />propanetricarboxylic acid, 2-(acetyloxy)-, tributyl ester, 2-methylhexadecan-1-ol,<br />1-docosene, 1-eicosanol and chloroacetic acid, octadecyl ester. However, the<br />ethanolic extract (CSE1) was characterized by the presence of fatty acids, fatty<br />acids ethyl esters and aromatic hydrocarbon where the most abundant compound<br />was n-tridecanoic acid ethyl ester.https://ejabf.journals.ekb.eg/article_17952_8959f14a4e4da52a3cd67a5cd546c3ce.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Geo-environmental study on mangrove swamps in some localities along the Red Sea coast of Egypt.23371950510.21608/ejabf.2018.19505ENAbd El Mohsen S. El DabaNational Institute of Oceanography and Fisheries, Red Sea Branch, Egypt.Mohamed Abd El WahabNational Institute of Oceanography and Fisheries, Red Sea Branch, EgyptJournal Article20180922Twenty one samples from the roots and leaves of <em>Avicenia marina </em>and the associated sediments were collected from two mangrove swamps along the Egyptian Red Sea coast for studying the heavy metals (Fe, Zn, Cu, Ni, Pb and Cd) accumulation and distribution. The results showed that the sediment samples have high concentration of heavy metals especially, the essential metals like Fe, than those recorded in roots and leaves of mangrove plants. Multi-statistical analyses Pearson’s correlation coefficients, CF, Igeo, BCF and TF analyses are widely used to distinguish the correlations among metals and the sources of these elements. Strongly positive correlations were observed in mangrove sediments between Zn and each of Cu, Ni as well as between Cu, Ni, Cd and Fe, in both studied sites indicating a good proxy for terrigenous material. The recorded levels of Fe, Zn, Cu and Ni in mangrove sediments were higher than the Red Sea, Gulf of Aqaba and Tanaznia sediments, while Pb and Cd were lower than those recorded from background continental crust and sediment quality guidelines (ERL).
The EF values for heavy metals indicated weak enrichment and reflect the local mineralogy rather than contamination. In addition, the values of CF indicated that the mangrove sediments of studied samples are low contaminated with Fe, Zn, Cu, Ni and Pb, while Cd value indicateds moderately contamination. Therefore, the average values of I<sub>geo</sub>in the two studied locations indicated unpolluted to moderately polluted sediments. The metal concentrations in these sites were either derived from similar sources of nearby Red Sea mountains or experienced analogous biogeochemical or accumulation processes. The obtained different correlations of heavy metals in the organs of mangrove plants may be attributed to the differences in the bioavailability of trace metals for the plants, the physicochemical properties of sediments or waters. BCFs and TFs values were basically less than one, except for TF of Ni and Pb as well as TF of Fe in two organs of mangrove at both studied sites, indicating that this mangrove species tend to restrict metal sediments-roots and roots-leaves transformations, guaranteeing the conduction of various important metabolic activities including photosynthesis in the above ground parts and confirmed that the <em>Avicennia marina</em> has the tendency to accumulate and translocate the heavy metals.https://ejabf.journals.ekb.eg/article_19505_ed736d2753a9c2acf9e8355035453d7c.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101A comparative biological study on Oreochromis niloticus from two Nilotic Canals in the Delta of Egypt39631795310.21608/ejabf.2018.17953ENElsayed A. KhallafFaculty of Science, Minufeya University, Shebeen Alkoom, EgyptMohammad M.N. AuthmanHydrobiology Department, National Research Centre, Dokki 12622, Giza, EgyptMansour GalalFaculty of Science, Minufeya University, Shebeen Alkoom, EgyptRania A. ZaidFaculty of Science, Minufeya University, Shebeen Alkoom, EgyptJournal Article20180919Bahr Shebeen canal (BSC) and Khadraweya canal (KHC) are two<br />Nilotic canals, the first is less polluted and the second is polluted.<br />Oreochromis niloticus is the major fish species in the Nile and its<br />branches, and consequently, this study is carried out on their biological<br />parameters are studied to investigate the effect of pollution in those<br />habitats. These included: various parameters of growth, mortality, survival<br />rates, stomach-somatic index, gonado-somatic index, and hepato-somatic<br />index. It was found that there is a significant decline in those parameters in<br />the fish of KHC, as compared to BSC, which could be tied to pollution in<br />that canal.https://ejabf.journals.ekb.eg/article_17953_0c911f9b27f9d1bbc4699b9539e67bac.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Growth, mortality and relative yield per recruit of the sharptooth catfish Clarias gariepinus (Clariidae) in Lake Manzalah, Egypt65721863510.21608/ejabf.2018.18635ENSahar MehannaFisheries management, National Institute of Oceanography and Fisheries0000-0003-4825-6403Ahmed F. MakkeyFish Population Dynamics Lab, National Institute of Oceanography and Fisheries, EgyptMohamed G. DesoukyFish Population Dynamics Lab, National Institute of Oceanography and Fisheries, EgyptJournal Article20180928Clariid catfishes are of great economic importance as protein food in adequate prices. For several years, species of the genus <em>Clarias</em> (Scopoli, 1777) have been used in local fish culture, where they proved to be a fast growing protein source. Age, growth, mortality and relative yield per recruit of <em>Clarias gariepinus</em> in Lake Manzalah, Egypt, were investigated. The maximum life span based on otoliths’ readings was 8 years, while the von Bertalanffy growth parameters were L∞= 86.88cm, K= 0.31/year and t<sub>0</sub>= -0.39 year. The mean annual instantaneous total, natural and fishing mortality coefficients were 0.98, 0.37 and 0.61/year, respectively. Accordingly, the exploitation ratio was 0.62, which was higher than the optimum one. Relative yield per recruit analysis revealed that <em>C. gariepinus</em> is over exploited in Lake Manzalah where the E<sub>0.5</sub> was lower than the current one (E<sub>0.5</sub> = 0.35). Some recommended management actions should be applied to strengthen the ban of fishing in the spawning season or in the nursery ground and to adopt a legal minimum size to protect the young and small-sized fish. In addition, to make any regulation more effective, all stakeholders (fishermen, scientists, non-governmental organizations and policy makers) should work together to establish a co-management plan to conserve this valuable stock.https://ejabf.journals.ekb.eg/article_18635_f35bda624931b75eb7d17e61b6e5348c.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Monitoring some pathogenic bacteria in water and fish of Lake Qaroun73851795510.21608/ejabf.2018.17955ENNoha M. SabryCentre of Excellence for Advanced Science (CEAS), Water Pollution Research
Department, National Research Centre, Giza, EgyptSahar T.MTolbaMicrobiology Department,
Faculty of Science,
Ain Shams University.
Abbassia Sq., Cairo, , Egypt .Fagr Kh. Abdel-GawadCentre of Excellence for Advanced Science (CEAS), Water Pollution Research
Department, National Research Centre, Giza, Egypt.Journal Article20180926Water quality plays a vital role in the productivity of lake’s fish. Lake<br />Qaroun is exposed to untreated agricultural, domestic and industrial<br />effluents which are considered serious pollutents. This study was<br />conducted to monitor the water quality of the lake and the pathogenic<br />bacteria in the surface water and fish. Physico-chemical parameters of<br />water and bacterial count of water and two types of fish Tilapia zillii and<br />Mugil cephalus were studied. Samples were collected from four sites at<br />certain distances from El-Batts drain in the lake during 2015-2016.<br />Physical characteristics of the water showed that the water temperature<br />varied between 16 and 30oC, pH was alkaline. Water salinity and disolved<br />oxygen decreased near El-Batts drain, while the chemical oxygen demand,<br />amonia and nitrate levels were high due to the pollution and bacterial<br />activity. Bacteriological characteriztion revealed that the bacterial load in<br />the nearest point to El-Batts drain was higher than the other examined sites<br />of lake water. The total and fecal coliform near El-Batts drain exceeded<br />the Egyptian standard limits; it reached 4.4x107 in summer. The study<br />revealed that the bacterial pathogens counts in fish were high in mullet<br />species than that of tilapia. Total and fecal coliforms were detected in<br />muscles once during the study period; in spring for tilapia and in summer<br />for mullet fish. The study revealed that Aeromonas hydrophila was the<br />most prevalant pathogen isolated from Tilapia zillii and Mugil cephalus<br />and that 72% of A. hydrophila harboured aerolysin toxin gene. A.<br />hydrophila was detected in muscles of both tilapia and mullet during the<br />four seasons through 12 months, while S. aureus and P. aeruginosa were<br />detected only in summer or spring. The study highlights the hazardous<br />effect of the untreated domestic and industrial drainage wastes on water<br />quality and fish of Lake Qaroun.https://ejabf.journals.ekb.eg/article_17955_bab273e4a50eb3a95c556d1a7f8cbf1f.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181115Impact of protected and non-protected lactic acid used as an acidifier in the diet on Oreochromis niloticus87971877010.21608/ejabf.2018.18770ENHassan M. SobhyGihan M. El MoghazyMohammed H. Abdel A’alHanaa E. IbrahimJournal Article20180917The present study was conducted to compare between protected and non-protected lactic acid on growth performance, feed utilization, some hematological parameters and carcass proximate analysis in <em>Oreochromis niloticus</em> (<em>O. niloticus</em>). Three iso-nitrogenous and iso-caloric diet contained (37.5% ± 0.97) crude protein (4427 cal/g ± 39) Gross Energy (GE) were formulated. The supplemented diet with 0.2% non-protected lactic acid (T1), 0.2 % protected lactic acid (T2) and diet with no additive (T3) were fed individually to three equal fish groups (25 fish/set up with an underlying body weight of 5.42 ± 0.07g) for 90 days. At the end of the feeding trial, <em>O. niloticus</em> offered the control diet exhibited lower growth and feed utilization rates than protected and non-protected lactic acid. Fish fed the diet T2 showed the highest final body weight (FBW), final weight gain (FWG), average daily gain (ADG), feed intake (FI) and survival rate (SR). Fish fed a diet (T2) showed improvement in the tested blood parameters compared to the control group. The present observations suggest that supplementation of lactic acid into the fish diet can be used as an acidifier for growth promoting purpose. In addition, protected lactic acid has a significant effect compared to the non-protected one.https://ejabf.journals.ekb.eg/article_18770_e5de2726340bec70243a032199271fa7.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Antimicrobial and anti-tumor activities of exopolysaccharides produced by the biofilm of marine Halomonas saccharevitans AB2 isolated from Suez Gulf, Egypt991192200710.21608/ejabf.2018.22007ENMohamed A. A. AbdraboMarine Microbiology Dept., National Institute of Oceanography and Fisheries, Alexandria, EgyptHassan A. H. IbrahimMarine Microbiology Dept., National Institute of Oceanography and Fisheries, Alexandria, EgyptSaharHassan WefkyMostafaHassanNational Institute of Oceanography and Fisheries (NIOF),
Marine MicrobiologyDep.,Kayet Bay, El-Anfushy, Alexandria, Egypt.0000-0001-8556-5833Usama M. Abdul-RaoufFaculty of Science, Al-Azhar University, Assiut Branch, EgyptJournal Article20180922Thirteen different attached marine bacteria were isolated from the surface of algae, rocksin biofilm forming device in Red Sea. They were screened for their potentiality in the exopolysaccharides (EPS) production. The most potent marine isolate 2D was identified by 16S rDNA analysis as <em>Halomonas saccharevitans</em> AB2 with 99% similarity<em>.</em> Optimum culture conditions which ease the synthesis of EPSsfrom <em>Halomonas saccharevitans</em> AB2 were evaluated. It grew well at 25˚C, pH 9 and on potato sea water extract with concentration 100%, lactose and malt extract as the best carbon and nitrogen sources<em>. </em>Application of Plackett-Burman experimental design achieved the maximum yield of EPSs (138 gl<sup>-1</sup>) with 1.4 fold increase when compared to growth under the basal conditions. The maximum yield of EPSs (153gl<sup>-1</sup>) with 1.6 fold increase was obtained upon applying Box-Behnken design. The EPSs showed antimicrobial activity by inhibition of different pathogenic bacteria and fungi with the maximum absolute unit (AU= 14.1 and 25.1) against <em>Vibrio fluvialis</em> and<em> Aspergillus niger</em> ATCC 16404, respectively. Moreover, these EPSs exhibited activities as antitumor agent against 8 cell lines with maximum inhibition (87%) at 50 μg with IC50 =20.3 μgml<sup>-1</sup>against human lung carcinoma (A-549). Potential antimicrobial and antitumor activities property of EPSs produced by <em>H. saccharevitans</em> AB2 may lead to the development of novel medical drugs.https://ejabf.journals.ekb.eg/article_22007_39bd73367386dd6d531d3670fc3f8cba.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101The use of freshwater crayfish Procambarus clarkii as an indicator of the bioavailability of some heavy metals in different watercourses in Egypt and the risk assessment of these metals1211351950910.21608/ejabf.2018.19509ENSoad S. Abdel GawadNational Institute of Oceanography and Fisheries, Cairo, EgyptAwaad A. M. El-SayedZoology Department, Faculty of Science (Boys), Al-Azhar University, CairoNeveen H. MahmoudZoology Department, Faculty of Science (Girls), Al-Azhar University, CairoFaten A. El-FiqyZoology Department, Faculty of Science (Girls), Al-Azhar University, CairoEman A. ShaabanNational Institute of Oceanography and Fisheries, Cairo, EgyptJournal Article20181012The present work is the second to the first one titled ''Bioaccumulation of Cadmium and Lead in the freshwater crayfish<em> Procambarus clarkii </em>from the River Nile, Egypt"(Shaaban <em>et al</em>., 2017). Crayfish <em>Procambarus clarkii </em>has been selected to assess some heavy metals accumulation pattern. This part aims to evaluate Iron, Zinc and Copper bio-accumulation in exoskeleton, muscles, hepatopancreas and ovary of this species as well as in water and sediment from the same sites for four seasons. Also, determination of risk assessment of Cadmium, Lead, Iron, Zinc and Copper. The results showed, Fe levels were higher than Zn and Cu concentrations in water, sediment and crayfish's organs in all sites. In water, the highest value of Fe was 1628.51μg/l recorded in El-Rayah El-Monoufy during summer, while Zn recorded highest value (60.6 µg/l) in spring at El-Rahawy. The highest value of Cu was 28.2µg/l at El-Warraq during summer. In sediment, Fe recorded the highest value (7005.5 μg/g) at El-Warraq during summer. Zn and Cu recorded the highest values (178.5 and 49.1 μg /g) at El-Rahawy during autumn and summer, respectively. In crayfish tissues, Fe was highest in the exoskeleton 126.13 μg/g at Helwan in summer while Cu and Zn were 37.17 and 43.74 μg/g respectively at El-Rahawy in autumn. On the other hand, the lowest concentrations of Fe, Zn, Cu were recorded in crayfish muscles (82.35, 37.85 and 17.14 μg /g respectively). For edible muscles, the results showed that, the values of Hazard index were within safe limits for Zn, Fe and Cu at all sites, while Cd values were detected only in El Rayah El Monoufy in safe limit. For Pb, HI values were unsafe at all sites. These results indicated, a larger amounts of crayfish will need to be eaten for health risk to be associated with Fe, Zn, Cu.https://ejabf.journals.ekb.eg/article_19509_847819a0a65b7355099fa6147c17d337.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Histological and ultrastructural alternations in the digestive gland of the Egyptian slug, Limax maximus (Linnaeus, 1758) treated with botanic molluscicidal thymol, with reference to biological studies1371481951210.21608/ejabf.2018.19512ENUstina N. T. HabibDepartment of Biological and Geological Sciences, Faculty of Education
Ain Shams University, Cairo, Egypt.Ahmed A. Abdel-HaleemDepartment of Biological and Geological Sciences, Faculty of Education
Ain Shams University, Cairo, Egypt.Omaima M. MustafaDepartment of Biological and Geological Sciences, Faculty of Education
Ain Shams University, Cairo, EgyptEman H. IsmailDepartment of Biological and Geological Sciences, Faculty of Education
Ain Shams University, Cairo, EgyptJournal Article20180917The present work included biological studies, morphological and anatomical features, beside histological and ultrastructural alternations due to effects of the botanical thymol, as poison bait, on the digestive gland of the Egyptian giant garden slug, <em>Limax maximus</em> (Family: Limacidae) after treating it with LC<sub>50</sub> and LC<sub>90</sub> for 48 hrs.Thymol is considered as a botanic molluscicidal mono-terpenoid, that is found in several plants. The estimated value of LC<sub>50</sub> and LC<sub>90</sub> of thymol are 269.77 and 362.79 ppm respectively. The effect of LC<sub>90</sub> on the digestive gland caused severe histological changes and ultrastructural abnormalities; as: cytoplasmic vacuolation, scattered toxic agents, degeneration of some nuclei and cells, rupture of microvilli, increasing of calcium spherules inside secretory cells and wide-fused vacuoles. So, thymol may be of great value in the field to control the target slug, as safe and economic molluscicide, which no harm upon ecosystems instead of using chemical pesticides that could pollute the environmenthttps://ejabf.journals.ekb.eg/article_19512_ccd0052b2d666146d48130728292f728.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Heavy metals in Fish Species from Mediterranean Coast, Tripoli Port (Libya): A comprehensive assessment of the potential adverse effects on human health1491641951410.21608/ejabf.2018.19514ENMohamed OkbahNational Institute of Oceanography and Fisheries, Egypt,marine chemistry lab.Edweb A. S. DangoHigh Institute in Jerpoly area, Tripoli, LibyaGehan M. El ZokmNational Institute of Oceanography and Fisheries, Kayet Bay, Alexandria, EgyptJournal Article20180922The Libyan coast plays an important role in terms of biodiversity and productivity of Mediterranean marine ecosystem. This study is designed to assess potential risks for human populations via fish intake. It enhances the information about anthropogenic impacts in Tripoli port (Libya) to understand the distribution of pollutants encourage appropriate common policies to predict potential risk zones for stakeholders. The levels of Iron, Zinc, Copper, and Cadmium in livers, gills, muscles, skin, and bones of five Mediterranean Sea fish species in Tripoli Port (Libya) namely; <em>Boops boops</em>, <em>Hemiramphus far, Sardinella aurita, Saurida undosquamis and Scomber japonicas </em>were evaluated. The results showed that considerable difference in metal concentrations among fish organs. The highest concentrations of Cd, Cu, and Fe were measured in livers, while gills and skins had higher concentrations of Zn. The ranges of heavy metal concentrations in different organs of studied fish species were reported (Zn; 7.18 - 21.94, Cu; 1.89-7.03, Fe; 0.93-4.05, Cd; 0.19 to 0.97 µgg<sup>-1</sup>wet wt.). Fortunately, the calculated weekly intakes of metals (EWIs) were much lower than provisional tolerable weekly intake (PTWI). So, human health risks resulting from consumption of studied fish species from Tripoli Port are inconsiderable, that fish muscles are not active tissues for metal accumulation, rendering them suitable for human consumption. In this study the hazard quotient (HQ) and the hazard index (HI) were <1, and so no potential health risk to the consumers. Principal component analyses specified that Cu and Cd were contributed from the same anthropogenic activity.https://ejabf.journals.ekb.eg/article_19514_77d1588b729d9e1c3e17fe309a729723.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Influence of different water resources on physical, chemical and heavy metals levels of El-Abbassa Fish Farms, El-Sharqia Governorate, Egypt1651742104110.21608/ejabf.2018.21041ENHala E. GhannamPollution Laboratory, Freshwater and Lakes Division, National Institute of
Oceanography and Fisheries (NIOF), Cairo, EgyptMohamed Y. M. AlyPollution Laboratory, Freshwater and Lakes Division, National Institute of
Oceanography and Fisheries (NIOF), Cairo, EgyptJournal Article20180929In this study the influence of various water origin on physical, chemical<br />and heavy metals levels of El-Abbassa fish farms, El-Sharqia governorate,<br />Egypt during experiment period for five months from March till July 2018<br />were investigated. The first fish pond was irrigated from Ismailia Canal,<br />which branched out from River Nile, while the second was supplied by<br />agriculture drainage water. Physical, chemical and heavy metals levels of<br />different water resources in different two fish ponds were monthly<br />determined, also gonadosomatic index of the ovary of Nile tilapia was<br />monthly determined. In addition, protein, cholesterol, carbohydrates and<br />glycogen were determined at the end of experiment period. The results<br />showed that, physical, chemical and heavy metal levels of different water<br />resources in two fish ponds of El-Abbassa fish farms were differed<br />significantly but not exceed the maximum permissible limits reported by<br />ECS, 1994 and USEPA, 2006 so, quality analysis of ponds water, fish species<br />and fish feeds during the breeding season must be performed continuously,<br />accurately according to national and international standard specifications to<br />ensure the success of the breeding process and to obtain the higher fish<br />production, furthermore, the levels of pollutants must be continuously<br />monitored in this important fish farms to improve the quality of the cultured<br />fish species.https://ejabf.journals.ekb.eg/article_21041_c827c6421e2685579ecf95e726753ac6.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Histochemical and fine structure studies of pituitary cell type distribution with special emphasis of gonadotrophs during annual reproductive cycle of the adult female white seabream (Diplodus sargus)1751871951610.21608/ejabf.2018.19516ENRania F. IsmailLaboratory of Fish Reproduction and Spawning, Aquaculture Division, National Institute of Oceanography & Fisheries, Kayet-bey, Alexandria, EgyptReham M. K. NegmLaboratory of Fish Reproduction and Spawning, Aquaculture Division, National Institute of Oceanography & Fisheries, Kayet-bey, Alexandria, EgyptJournal Article20180919The ovarian cycle of the female white seabream (<em>Diplodus sargus</em>) can be explained as five stages as follows: Immature, Maturing, Nearly ripe , Ripe and Running and Spent stage, whereas the peak of gonadostmatic index was reported between January to April, with an average GSI value of 2.64 ± 0.6. The adenohypophysis of white seabream was investigated using histochemical and electron microscopy techniques. It can be subdivided into three distinct regions: rostral pars distails (RPD), proximal pars distails (PPD) and pars intermedia (PI). Six cell types were identified and localized in the adenohypophysis. The acidophilic prolactin (PRL) and adrenocorticotropic hormone secreting cell (ACTH) were found in the RPD region. The ACTH cells were generally arranged between neurohypophysis and PRL cells. The melanocyte stimulating hormone secreting cells (MSH) and somatotrops cells (STH) were acidophilic and detected in the PPD and RPD regions. The basophilic cells were gonadotrophic cells (GTH) and the thyrotrophic cells (TSH); one type of the GTH cells was detected in <em>D. sargus </em>pituitary. The GTH cells displayed variations through the reproductive cycle, while all four types of acidophilic cell and TSH cells did not show any variations in size and activity throughout the reproductive cycle. During immature and mature stages GTH cells showed the minimum granulation and smaller cell size, at nearly-ripe stage the GTH cells were characterized by intense accumulation of granules and reached to maximum diameters (p<0.05) as demonstrated by electron microscope. During ripening and running stage (from January to April), slight decrease in the GTH cell diameters and its staining affinity and granulation were observed. During the spent stage (from April to June) the GTH cells displayed faint stain with presence of vacuoles between cells with minimum amount of granulation.https://ejabf.journals.ekb.eg/article_19516_956844d0f6e6424ca5d83686531d6f26.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101The role of dietary astaxanthin in European sea bass (Dicentrarchus labrax) growth, immunity, antioxidant competence and stress tolerance1892002104410.21608/ejabf.2018.21044ENNorhan Salehfish nutrition, national institute of oceanography and fisheries, Alexandria, EgyptElham A. WassefFish Nutrition Laboratory, Aquaculture Department, National Institute of
Oceanography and Fisheries (NIOF), Alexandria, EgyptShymaa M. ShalabyAquaculture Department, Faculty of Fish Resources, Suez University, Suez,
EgyptJournal Article20180928European sea bass (Dicentrarchus labrax) fingerlings (0.4 ± 0.05 g<br />initial weight) were fed on 4 diets contain 0, 60, 80 and 100 mg axtaxanthin<br />/kg diet for 60 days. Results showed marked enhancement in fish growth,<br />feed utilization efficiency, survival rate and fish protein content when 100 mg<br />astaxanthin was supplemented in fish diet. The activities of hepatic<br />antioxidation enzymes; superoxide dismutase (SOD) and glutathione<br />peroxidase (GPx) have reduced as the level of dietary astaxanthin increased.<br />Results showed simultaneous gradual increase in intestinal mucosal<br />phagocytic and lysozyme activities as astaxanthin inclusion level elevates in<br />diets indicating effective role of astaxantin as an immunostimulant agent in<br />sea bass diet. By the end of the trial, fish were exposed to a sudden drop in<br />water salinity (37 to 0.3‰) and that continued for 24 hours period. Survival<br />rate was significantly the highest in fish that consumed 100mg/kg diet and<br />when compared with control group an increment of 36.9% was recorded<br />suggesting an improvement in fish tolerance against osmotic stress. Results<br />demonstrate that astaxanthin is a qualified feed additive for sea bass.https://ejabf.journals.ekb.eg/article_21044_7b2ec7fbf68ed8c8eec6f441e31aa0e0.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Physa acuta snail as a biomonitor for the efficacy of bioremediation treatment of heavy metals (Fe III and Cd II) using the fungus (Eupenicillium lapidosum) in lined and unlined laboratory conditions2012182050710.21608/ejabf.2018.20507ENGamalat Y. OsmanZoology Department, Faculty of Science, Menoufia University, EgyptAzza H. MohamedZoology Department, Faculty of Science, Menoufia University, EgyptAhmed Abdel KaderEnvironmental Research and Medical Malacology Department, Theodor Bilharz Research Institute, Imbaba, Giza, EgyptMohamed M. GhariebMicrobiology Department, Faculty of Science, Menoufia University, EgyptAsmaa Abdel-MotlbEnvironmental Research and Medical Malacology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt0000-0001-5050-8046Journal Article20180912The present study aimed to evaluate the effectiveness of<em> Physa acuta</em> snail as biomonitor for the efficacy of bioremediation treatment of heavy metals (Fe III and Cd II) using fungus <em>Eupenicillium lapidosum</em>.So,the effects of these heavy metals on the mortality percent of adult <em>P. acuta</em> snail, heavy metal concentrations in their soft tissuesand on the antioxidant enzymes were evaluated under laboratory lined (sand cement) and unlined (mud) conditions for 2, 24 hrs and 7 days of exposure to the fresh mycelia of the fungus <em>E. lapidosum</em>, (50 ppm of Fe (III) & Cd (II)) and biosorption treatments of Fe and Cd by the fungus<em> E. lapidosum</em>. The data indicated that Cd was somewhat more toxic to the snails than Fe in cement lining than that of unlined conditions (mud). While, the lowest mortality percent was that of the biosorption treatments against tested heavy metals. The metal biosorption values by <em>E. lapidosum</em> against Cd were higher than that of Fe in cement lining than that of unlined ones. Concerning the results of iron and cadmium analysis in soft tissues of <em>P. acuta</em> snails demonstrated that the iron concentrations were higher than that of cadmium. While, during the biosorption processes the concentrations of accumulated metals were decreased by increasing the time of exposure. The data of the biochemical responses in tissues of exposed <em>P. acuta</em> snails showed alterations of some antioxidant parameters which was indicated by the significant increase in the lipid peroxidation (MDA) and decrease of endogenous antioxidant enzymes; Catalase (CAT) and glutathione (GSH) in lined conditions than that of unlined ones, while the effect decreased in the case of Fe and Cd biosorption. So, these results indicated that <em>P. acuta</em> snail and the fungus <em>E. lapidosum</em> are useful in the searches for biomarkers and biosorption, respectively.https://ejabf.journals.ekb.eg/article_20507_4899e9235596d2f9963baf58f7751378.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Effect of weaning time on growth, body composition, fatty acids and digestive enzyme activities of European seabass (Dicentrarchus labrax) larvae2192301952110.21608/ejabf.2018.19521ENAhmed MohamedSalemFish Physiology Laboratory, Fisheries Division, National Institute of Oceanography and Fisheries (NIOF), Ministry of Scientific Research, Alexandria, Egypt.0000-0002-5904-3633Shaimaa M. HebalahFish Nutrition Lab., Aquaculture Division, NIOF, Alexandria, Egypt.Hassan A.H. IbrahimMarine Microbiology Lab., Marine Environment Division, NIOF, Alexandria, Egypt.Einar RingøNorwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiTThe Arctic University of Norway, Tromsø, Norway0000-0002-4940-5688Journal Article20180915The present study addressed to investigate early, 25 days post hatching (dph) and late co-feeding time; 35 dph on European seabass (<em>Dicentrarchus labrax</em>) larvae. Initially, larvae were reared from the 5<sup>th</sup>dph in six glass aquaria, and each aquarium contained 200 larvae.The results revealed higher, but no significant (P > 0.05) effect on larval final weight, weight gain, average daily gain and specific growth rate when larvae were co-feed at 35 dph. However, the highest survival rate was noticed then larvae were co-fed from 25 dph. Growth parameters showed significant (P < 0.05) effect on final gainsof both total and standard lengthswhen larvae were started co-feeding at 25 dph. The water quality showed no significant (P > 0.05) differences between treatments. However, significant (P < 0.05) effect was revealed on total culturable bacteria - and bacilli counts in water samples at 45 dph,when larvae were co-fed at 25 dph, but no significant (P > 0.05) effect was noticed on <em>Staphylococcus</em> counts at 45dph of larvae co-fed from 35 dph. Digestive enzymes analysis revealed that lipase activity was not significantly (P > 0.05) affected, however, a significant (P < 0.05) increase in amylase activity was revealed when larvae co-fed at 35 dph.https://ejabf.journals.ekb.eg/article_19521_611bd2b5e8c002d7d412f168c3ed9fed.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181201Influence of saponin fraction from Albizia anthelmintica on Biomphalaria alexandrina snail; the intermediate host of Schistosoma mansoni in Egypt2312402044610.21608/ejabf.2018.20446ENDina M. BahgatHanan S. MossalemEman Al-SayedOmayma A. EldahshanAbdel Nasser B. SingabHanaa M. Abu El EininJournal Article20180916Saponin fraction from<em> A. anthelmintica</em> has a promising molluscicidal effect against snails for LC<sub>50</sub> of 17.6 ppm as well as a strong biocidal activity against the larval stages of <em>S. mansoni </em>(miracidia and cercariae). A high significant decrease (p<0.001) was recorded in infection rate and survival rate during continuous exposure of the plant; being 50±10 and 44.66±5.05 respectively compared with 81±10.1 in control groups respectively. Light microscopic investigation showed that the tested plant is responsible for the increase of snail's amoebocytes that is consider a main component of the internal defense system of the <em>B. alexandrina</em> snails. Electron microscopic examination showed remarkable apoptotic effects, nuclear chromatin condensation, vacuolated cytoplasm and deterioration in the internal organs as endoplasmic reticulum. It is concluded that exposure of <em>B. alexandrina</em> snails to saponin fraction from <em>A. anthelmintica</em> may be used as a promising molluscicidal and biocidal against the <em>S. mansoni</em> parasite.https://ejabf.journals.ekb.eg/article_20446_269ddff99bb9d18a05a2ccffb56e083b.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181201Biometric variables and relative growth of the date mussel Lithophaga lithophaga (L., 1758) (Bivalvia: Mytilidae) from the Eastern Mediterranean Sea, Egypt2412482206210.21608/ejabf.2018.22062ENSomaya M.T.National Institute of Oceanography and Fisheries, EgyptFatma Aly Abdel RazekNational Institute of Oceanography and FisheriesAmal R. KhafageNational Institute of Oceanography and Fisheries, EgyptHamdy A. OmarNational Institute of Oceanography and Fisheries, EgyptRabab S. El-DeebNational Institute of Oceanography and Fisheries, EgyptJournal Article20181106The present study was conducted to determine the biometric features of the Egyptian population of the date mussel <em>Lithophaga lithophaga</em> and the relationships between them. Over the study period, 746 samples were collected from the shallow area of Alexandria coastal line (3-6 m depth) by SCUBA diving from December, 2017 to November, 2018. The length frequency analysis of all the samples collected revealed that the most abundant shell length ranged from 45.0 to 50.0 mm amounting to19.71% of the population. The specimens ranged from 11.9 mm (minimum length) to 83.99 mm (the largest length) and 0.40 g to 40.1 g in weight. The current study is focusing on the relationships between length-width, height and length-weights (length-total wet weight, length-tissue dry weight and length-shell dry weight). All relations were of negative allometry growth pattern. The relationships between length and height/width were linear, while those between length-weights followed a non-linear pattern. Moreover, the results revealed high correlation coefficients between these shell morphological characters of the present studied population in the Egyptian waters. Moroever, peak values of meat yield (MY) and condition index (CI) were found in July, which is indicative of the nutritive status of <em>L. lithophaga</em> mussel. The mean values were from 23.91±5.61 to 47.32±5.14 and from 24.23±8.69 to 44.0±11.0 for meat yield and condition index, respectively. The results of the current investigation are valuable information about this species in the studied area, however, more research is required for a better understanding of the reproductive characteristics of <em>L. lithophaga</em> and the long term effects of its burrowing behavior on the ecosystem.https://ejabf.journals.ekb.eg/article_22062_c8bd96e30501259d2c59927ca6fc8d5c.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181201Fisheries status of the common species of family Mullidae in the Southern Red Sea, Egypt2492652201710.21608/ejabf.2018.22017ENFarrag M. M. S.Department of Zoology, Faculty of Science, Al-Azhar University (Assiut branch), Assiut, EgyptOsman A. G. M.Department of Zoology, Faculty of Science, Al-Azhar University (Assiut branch), Assiut, EgyptMehanna S. F.National Institute of Oceanography and Fisheries, Suez, EgyptOsman Y. A. A.National Institute of Oceanography and Fisheries, Hurghada, EgyptJournal Article20180912The population structures and stock status of the goatfish (Family; Mullidae) were investigated from Hurghada, Red Sea, Egypt since these kinds of fish are important in different fields, such as fisheries, tourism…,etc. The fish samples were monthly collected from Hurghada harbor during the year 2016. The data available in the harbor and in the lab were taken for all species and the analysis was conductedfor the common three only. As a result, the goatfish was mainly caught by gillnet and trammelnets in the study area, and they were classified into seven species;<em> Mulloidichthys flavolineatus</em> (Lacepède, 1801); <em>M. vanicolensis</em> (Valenciennes, 1831); <em>Parupeneus forsskali</em> (Four-manoir & Guézé, 1976); <em>P. macronema</em> (Lacepède, 1801); <em>P. cyclostomus</em> (Lacepède, 1801); <em>P.rubescens</em> (Lacepède, 1801); <em>P. heptacanthus</em> (Lacepède, 1801). The catch compositionof the Goatfish speciesby number showed that <em>M. flavolineatus</em> was the most abundant species, constituted of 43%, followed by <em>P. forsskali</em>, and <em>M. vanicolensis,</em> constitutedof 19 %, and 12% respectively. Themaximum length and weight, and minimum length and weight of species and their seasonal occurrence, confirmed that the most common and dominant species were <em>M. flavolineatus, P. forsskali</em>; <em>M. vanicolensis</em>.The length frequency of <em>M. flavoli-neatus</em> ranged between11and 33.9cm TL, and the most dominant length group were at 19-19.9 cm TL. The length of <em>M. vanicolensis</em> ranged between 15 and 31 cm TL, and the most occurred lengths were at 27 cm TL. On the other hand, the length frequencies of <em>P. forsskali</em> varied between 11 and 27.9 cm, and the highest frequencies were at 18-18.9 cm TL. Sex ratio was 1:0.86, 1:0.47 and 1:1.01 for <em>M.</em><em> </em><em>flavolineatus</em>, <em>M.</em> <em>vanicolensis</em> and <em>P. forsskali</em>, respectively. The mean total mortality coefficient "Z<span style="text-decoration: line-through;">,</span>" estimated by two different methods, were found to be 1.49, 1.43 and 1.09year-<sup>1</sup> for<em>M.</em><em> </em><em>flavolineatus</em>, <em>M.</em><em>vanicolensis,</em> and <em>P. forsskali</em> respectively. The natural mortality coefficient "M" was estimated to be 0.48, 0.64 and 0.55year-<sup>1 </sup>for <em>M.</em><em> </em><em>flavolineatus</em>, <em>M.</em><em> vanicolensis,</em> and <em>P. forsskali,</em> respectively. Exploitation rate "E" was computed as 0.68, 056 and 0.49 respectively. The relative yield of <em>M. flavolineatus</em>should bereduced from 0.68 to 0.33 (45%), from 0.56 to 35 (39 %) for <em>M. vanicolensis,</em> andfrom 0.49 to 0.33 (32%) for <em>P. forsskali</em> to maintain a sufficient spawning biomass.https://ejabf.journals.ekb.eg/article_22017_db51f46387e5477e87069b20b401bf49.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190101Effect of Methyl Farnesoate Administration on Ovarian Growth and Maturation in the Freshwater Crab Travancoriana schirnerae2572712418910.21608/ejabf.2019.24189ENSudha Devi Arath RaghavanAswani AyanathJournal Article20180925This study assessed the effect of exogenous administration of methyl farnesoate on growth and maturation of ovary in the freshwater crab <em>Travancoriana schirnerae</em>. Results provide strong evidence that methyl farnesoate administration induced ovarian growth in all phases of oogenesis as judged by the increased ovarian indices, oocyte diameter and oocyte proportion values of experimentals over controls. Our observations also revealed that methyl farnesoate treatment induced avitellogenic and previtellogenic ovaries to grow into vitellogenic and early vitellogenic to middle vitellogenic ovaries as evidenced by the presence of a large number of vitellogenic oocytes in avitellogenic and previtellogenic ovaries and enhanced yolk deposition in early vitellogenic ovaries. However, methyl farnesoate injection during late vitellogenic phase effected no significant change in morphology or histology of the ovary. From the results it is evident that methyl farnesoate administration caused a marked increase in gonial proliferation in both reproductive and non-reproductive phases. The elevated ovarian index, mean oocyte diameter and oocyte proportion values of experimentals over controls in the present investigation may be attributed to the increased growth and yolk deposition in oocytes triggered by methyl farnesoate. It is concluded that methyl farnesoate administration is effective in inducing ovarian growth and maturation thereby reducing the duration of the ovarian cycle which is highly advantageous for a species of aquaculture potential.https://ejabf.journals.ekb.eg/article_24189_3d4ea093be491db06e1fed0dfecdad4d.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181101Effect of technological processing on the safety of Indian mackerel (Rastrelliger kangurata) from Suez, Egypt2832942200910.21608/ejabf.2018.22009ENHesham F. AminDepartment of Fish Processing and Technology, Faculty of Fish Resources, Suez University, Suez, Egypt.Omaima M. AhmedDepartment of Fish Processing and Technology, Faculty of Fish Resources, Suez University, Suez, Egypt.Abdel-hamied M. RasmeyDepartment of Botany and Microbiology, Faculty of Science, Suez University, EgyptAlaa YounisDepartment of Aquatic Environment, Faculty of Fish Resources, Suez University, Suez, Egypt0000-0002-8669-4191Alaa El-Din A. BekhitdDepartment of Food Science, University of Otago, Dunedin, New Zealand.Journal Article20181103This study investigated the effect of different fish processing technologies on the bacteriological safety and heavy metal contents of Indian mackerel fish products. Mackerel was processed by salting (dry salt 15%), hot marinating (2.5% acetic acid and 3% NaCl) or canning (hot vegetable oil, brine 3% or tomato sauce 8%). Fresh, chilled fish from the local market had an aerobic colony count of 5.1± 0.2 logs CFU/g and the presence of <em>Salmonella enterica</em> was confirmed using API diagnostics and 16S rDNA. Marination and salting resulted in 4 and 1.3 logs CFU/g reduction in aerobic colony counts, respectively. Canning eliminated all viable bacterial growth, regardless of filling medium (vegetable oil, brine or tomato sauce). Processing technologies had no effect on the heavy metal (Hg, Cd, Pb, and Cu) content of the fish products.The estimated daily and weekly intake values of Pb and Cd from fresh fish and their products were below the recommended safety regulations. Canning, followed by marination, were the safer options for the preservation of Indian mackerel (compared to salting).https://ejabf.journals.ekb.eg/article_22009_87481d1fb285129b62bc1e1fdefc4735.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181230Some biological aspects and population dynamics of the gilthead sea bream from Bardawil lagoon, Sinai, Egypt.2953082392310.21608/ejabf.2018.23923ENAl-Zahaby A. S.Department of Zoology, Faculty of Science, Zagazig University, Zagazig, EgyptMohamed AhmedEl-drawanyDepartment of zoology, Zagazig university0000-0002-8550-144xMahmoud H. H.Fisheries Technology and Aquaculture College, Arab Academy for Science and Technology, Alexandria, Egypt.Abdalla M.A.FFishery Biology Lab, National Institute of Oceanography and Fisheries, Cairo, Egypt.Journal Article20181027A total of 856 gilthead sea bream, <em>Sparus aurata</em> (Linnaeus, 1758), was collected from different landing sites of Bardawil Lagoon between October 2011 to September 2012, in order to investigate its population dynamics. The age distribution covered age groups 0 to IV. Total length ranged from 10 to 34.2 cm while total weight varied from 10.8 to 568 g. The length-weight relationship parameters were a = 0.0146 and b = 3.0062. The estimated parameters of the von Bertalanffy growth function (VBGF) in length were K = 0.370 year<strong><sup>-1</sup></strong>, L∞ = 39.17 cm and t<strong><sub>o</sub></strong> = -0.65 years. The estimated total length at first capture (Lc) was 12.85 cm, while the total length at 50% maturity (Lm) was 22.7cm. The rates of total mortality (Z), natural mortality (M) and fishing mortality (F) were 1.085, 0.394 and 0.691 year<sup>-1</sup>, respectively. The exploitation ratio (E) was estimated as 0.637, so the finding result revealed that the stock of <em>S. aurata</em> in Bardawil Lagoon is heavily exploited.https://ejabf.journals.ekb.eg/article_23923_80ea97d848dcb907d7930b5ce42d5724.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181201Pathological impacts of environmental toxins on Oreochromis niloticus fish inhabiting the water of Damietta branch of the River Nile, Egypt.3093212566010.21608/ejabf.2018.25660ENSafaa I. TayelAquaculture division, Fish pathology lab, National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.Soaad A. MahmoudAquaculture division, Fish pathology lab, National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.Nasr A.M. AhmedAquaculture division, Fish pathology lab, National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.Amina Abdel MoneamAbdel Rahmanwomen's college, ain shams university, cairo, egyptJournal Article20180802<span>The present study was carried out at El-Kanater El-Khayria & Talkha stations, Damietta branch of the River Nile. The concentrations of heavy metals (iron, zinc, copper, cadmium and manganese) in water and their accumulations in organs (muscles, gills, liver and kidneys) of <em>Oreochromis niloticus</em>fish were determined seasonally during year 2016. Also, the histopathological</span><span> alterations</span><span> in the same organs of selected fish samples were studied during the same year. The obtained results showed an increase in heavy metals concentrations in water collected from investigated area and these </span><span>concentrations followed an abundance of: Fe</span><span>></span><span> Mn </span><span>></span><span> Zn </span><span>></span><span> Cu </span><span>></span><span> Cd</span><span>. These metals accumulated in some organs of the studied fish and caused histopathological alteration in these studied organs</span><span>.</span><span> The histopathological alteration included degeneration, necrosis, edema, hemorrhage, hemolysis, hemosiderin, parasitic forms and hyperplasia in selected organs.</span><span> Those changes were observed in all studied organs at the two stations during the period of study with severe degree at Talkha station during hot seasons. So, it is necessary to treat the polluted water before its discharging into the Damietta branch to protect fish and human beings from the dangers of pollution. </span>https://ejabf.journals.ekb.eg/article_25660_9d37abe0a30b2bd4367f99b6f3a11bbb.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181220Stock assessment and management of the rabbitfish Siganus rivulatus from the Southern Red Sea, Egypt3233292206110.21608/ejabf.2018.22061ENSahar MehannaFisheries management, National Institute of Oceanography and Fisheries0000-0003-4825-6403Ashraf S. MohammadFisheries management, National Institute of Oceanography and FisheriesSamia M. El-MahdyNational Institute of Oceanography and Fisheries, EgyptYassein A.A. OsmanNational Institute of Oceanography and Fisheries, EgyptJournal Article20181112Rabbitfishes, family Siganidae, are one of the most abundant fishes inhabiting the Red Sea and now widely distributed in the Mediterranean. Age and growth of <em>Siganus rivulatus, </em>the most common species, were studied from samples collected randomly between October 2017 and October 2018 from Shalatein landing site. Otoliths of 2000 specimens were used for age determination and the maximum attained age for this species was four years. The von Bertalanffy growth parameters L<sub>∞</sub>, K and t<sub>o</sub> and the instantaneous annual rates of total, natural and fishing mortality were estimated and consequently the exploitation rate was determined. It is found that the current exploitation level is higher than the optimum one reflecting the intensive exploitation of <em>S. rivulatus</em> in the Southern Red Sea, Egypt. Yield per recruit analysis revealed that fishing effort should be decreased. This can be adopted through closed seasons/areas, increase of mesh size, increase of minimum landing size and setting of a total allowable catch (TAC).https://ejabf.journals.ekb.eg/article_22061_9dbc911c4cfbd1c22252c279179cb6aa.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181220Eutrophication assessment using TRIX and Carlson’s indices in Lake Mariout Water, Egypt.3313492391810.21608/ejabf.2018.23918ENGehan M. El ZokmMarine Chemistry Laboratory, Marine Environment Division, National Institute of Oceanography and Fisheries, Alex., EgyptHermine R.Z. TadrosMarine Chemistry Laboratory, Marine Environment Division, National Institute of Oceanography and Fisheries, Alex., Egypt.Mohamed A. OkbahMarine Chemistry Laboratory, Marine Environment Division, National Institute of Oceanography and Fisheries, Alex., Egypt.Galila H. IbrahimMarine Chemistry Laboratory, Marine Environment Division, National Institute of Oceanography and Fisheries, Alex., Egypt.Journal Article20181012Eutrophication of coastal waters is considered one of the major threats in marine ecosystems. Therefore, continuous monitoring is urgently needed to define potential risk zones. The present study is an attempt to test the applicability of the trophic state indices (TRIX) and Carlson’s indice for scaling the eutrophication status of Lake Mariout in concert with a number of physicochemical descriptors aimed to select the most relevant of their ecological quality directed to increase fish production. Water samples were investigated seasonally during 2014. The range and annual average values of Physicochemical parameters were 18-27.5°C (23.20±0.52°C) for Temperature; 0.28-7.40‰ (3.70‰±1.69‰) for Salinity; 7.84-8.84 for pH; 0.60 - 5.24meq/l (1.97 ±0.43meq/l) for Total alkalinity; 0.14–10.71mg/l (4.91±2.98mg/l) for DO; 0.01-2.6mg/l (1.35±0.32mg/l) for BOD; 3.2-134.4mg/l (56.35±27.89 mg/l) for OOM; and 16.30–161.00 mg/l (63.80±24.70) for Total suspended solids;. The results of nutrient salts range and mean concentration (μM) were from 0.20 to 118.30 (15.61±15.46) for Ammonium; from 0.05 to 22.48 (3.43±3.38) for Nitrite; from 0.14 to 66.67 (13.50±7.78) for Nitrate; from 0.39 to 157.26 (39.28±43.79) for DIN; from 0.32 to 123.55 (33.64±18.77) for total nitrogen; from 0.10 to 45.30 (8.27±10.28) for reactive phosphate; from 0.04 to 49.60 (7.98±6.93) for total phosphorus; and from 2.14 to 226.16 (39.97±21.85) for silicate. Chlorophyll-a ranged from 3.95 to 130.3µg/l (28.51±21.99µg/l). According to seasonally calculated TRIX and Carlson’s trophic state indices values, the sites are ranked in a descending order from very high to moderate eutrophic level for most stations, revealing the existence of anthropogenic stress.https://ejabf.journals.ekb.eg/article_23918_48ea820ffd64b3e63fdf13f6fedc64e0.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181215Production and quality evaluation of hot smoked grass carp (Ctenopharyngodon idella) fillets stored at 4±1°C3513612392410.21608/ejabf.2018.23924ENSabri M. A. ShehataMarine Biology Branch, Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.Mohammed H. M. GhanemMarine Biology Branch, Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.Abdelrahman S.A. TalabFish Processing and Technology Laboratory, Fisheries Division, National Institute of Oceanography and Fisheries (NIOF), Cairo, EgyptMahmoud M. M. AbbasMarine Biology Branch, Zoology Department, Faculty of Science, Al-Azhar University, Cairo, EgyptJournal Article20181102The present study assessed the effect of hot smoke at 50-90°C for 5-6 h using hard sawdust of beech wood and two salt levels (10% and 16%) on keeping quality of grass carp,<em> Ctenopharyngodon idella,</em> fillets stored at 4±1°C. Chemical composition, physiochemical aspects, minerals and sensory analysis were determined.<br /> Results showed that, the mean values of moisture, proteins, lipids, carbohydrates, ash, calorific value, pH, TVBN and TBA were 78.11±0.69, 16.55±0.84<strong>, </strong>2.31±0.01, 1.16±0.01<strong>, </strong>1.87±0.01, 91.63±1.49, 6.55±0.01, 4.43±0.05 and 0.43±0.01, respectively for raw grass carp<strong>; </strong>while it was recorded48.22±0.23, 23.38±0.18<strong>, </strong>13.88±0.11<strong>, </strong>8.14±0.01, 6.38±0.09<strong>, </strong>251.05±1.33, 6.15±0.05, 12.42±0.03 and 2.54±0.05, respectively for hot smoked grass carp fillets with 10% salt concentrations and 48.01±0.23<strong>, </strong>23.01±0.18, 14.66±0.17, 5.41±0.03, 8.50±0.03, 247.27±2.22, 5.52±0.03, 8.88±0.02, 2.04±0.07, for the hot smoked grass carp fillets with 16% salt concentrations, respectively. Mean values of the tested minerals and heavy metals (mg/100g, on dry weight basis) of raw; hot smoked fillets with 10% and 16% salt concentrations were as follows: Ca (170±0.27<strong>,</strong> 233±1.11<strong>, </strong>236±1.21)<strong>; </strong>K (197±0.26<strong>, </strong>349±3.01, 448±4.55), P (187±0.12<strong>, </strong>343±3.33<strong>, </strong>375±4.88), Na (93.80±0.35<strong>, </strong>103±12.76<strong>, </strong>115±12.77), Cd (0.07±0.001, 0.023±0.001, 0.020±0.001); Cu (1.33±0.01, 2.46±0.01, 3.46±0.04); Fe (25.15±0.47, 60.87±0.21, 69.23±1.31); Mn (0.98±0.01, 0.78±0.001, 0.76±0.02); Ni (0.64±0.003, 0.77±0.001, 0.60±0.001) and Zn (9.92±0.51, 24.38±0.04, 18.80±0.05), respectively. Statistical analyses showed that, moisture, pH value, TVBN and TBA were significantly increased with the increasing storage time, while, proteins, lipids and ash were significantly decreased. Physicochemical aspects and sensory scores showed that, 16% salted hot smoked fish was better than 10% with prolong the shelf life of hot smoked grass carp to 40 days of cold storage.https://ejabf.journals.ekb.eg/article_23924_122012ddfa2b803013bd5e503db50f70.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181230Restriction Fragment Length Polymorphism (RFLP) and Morphometric Studies of Two Morphologically Confused Crabs from Abu Quir, Alexandria, Egypt.3633752401110.21608/ejabf.2018.24011ENTarek G. AliZoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.Journal Article20181022Two types of morphologically confusedcrabs were collected from Abu Quir, Alexandria, Egypt, <em>Callinectes spidus</em> and <em>Callinectes ornatus</em>. The carapace of these crabs was first removed, and the abdominal muscles were fixed in absolute ethyl alcohol. DNA was extracted and restricted by three enzymes, and RFLP technique was conducted. Molecular weight, optical density, flowing bands and Gel-Pro-Analyzer technique were also applied. Morphometric analysis of the two species was applied on the carapace width, carapace length and body weight.
The estimation of similarity index applied on the two species was 0.59. In addition, morphometric studies showed no significant difference in the body weight and carapace length of all samples of both males and females. On the other hand, the mean width of carapace of 16.7% of the male samples showed significant difference, while the rest of male and female samples (83.3%) showed no significant difference. In conclusion, the result of RFLP together with the morphometric data may suggest that the two studied crabs belong to one species.https://ejabf.journals.ekb.eg/article_24011_57c2f73eae9fc90298f8da8048607e2d.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181225Effect of stocking density and feeding rate of Oreochromis niloticus on water quality and abundance of phytoplankton in aquaculture earthen ponds3874022217510.21608/ejabf.2018.22175ENAhmad M. AzabZoology Depart., Faculty of Science, Al-Azhar University, Cairo, EgyptHassan M. M. Khalaf-AllahZoology Depart., Faculty of Science, Al-Azhar University, Cairo, EgyptAbd El-Rahman A. KhattabyCentral Laboratory for Aquaculture Research - Abbassa, Sharkyia Governorate, EgyptAhmed S. M. SadekGeneral Authority for Fish Resources & Development, EgyptEnas Galal MansourCentral Laboratory for Aquaculture Research - Abbassa, Sharkyia Governorate, EgyptMohammed O. M. Abdel-GhanyGeneral Authority for Fish Resources & Development, EgyptJournal Article20181105The present work aimed to study the effect of stocking density and feeding rates of <em>Oreochromis niloticus </em>on water quality and abundance of phytoplankton in earthen ponds<em>. </em>This experiment was conducted in 12 earthen ponds at Parsik Culture, Idko, Behira Governorate, Egypt. The ponds have the same area of about 2 feddan with an approximately average depth of 1m. Monosex fries of <em>Oreochromis niloticus </em>reared for 214 days with different stocking densities and feeding rates (<strong>T<sub>1</sub></strong>: stocking density of 6 fish/m<sup>3</sup> with feeding rate of 2.5%; <strong>T<sub>2</sub></strong>: stocking density of 6 fish/m<sup>3</sup> with feeding rate of 3.5%; <strong>T<sub>3</sub></strong>: stocking density of 8 fish/ m<sup>3</sup> with feeding rate of 2.5% and <strong>T<sub>4</sub></strong>: stocking density of 8 fish/ m<sup>3</sup> with feeding rate of 3.5%), each treatment had three replicates.
Results showed that the highest average values of salinity(‰), total dissolved solids (mg/l), hydrogen ion concentration, total hardness (mg/l) and total nitrite (mg/l) were recorded in <strong>T<sub>1</sub></strong> and <strong>T<sub>2</sub></strong> (low stocking fish densities); while their lowest values were recorded in <strong>T<sub>3</sub></strong> and <strong>T<sub>4</sub></strong> (high stocking fish density). On the other hand, the highest average values of chlorophyll a (μg/l) were recorded in <strong>T<sub>3</sub></strong> and <strong>T<sub>4</sub></strong> and their lowest values were recorded in <strong>T<sub>1</sub></strong> and <strong>T<sub>2</sub></strong>.The highest average value of dissolved oxygen content (mg/l) was recorded in <strong>T<sub>2</sub></strong> and <strong>T<sub>4</sub></strong> (high feeding rate), while the lowest average was recorded in <strong>T<sub>1</sub></strong> and <strong>T<sub>3</sub></strong> (low feeding rate). Total nitrate (mg/l) and orthophosphate had the reverse trend of these results. The maximum average value in abundance of phytoplankton specimens was recorded in <strong>T<sub>4</sub></strong> (222728667±37584559 organism/l) and the lowest occurred in <strong>T<sub>2</sub></strong> (156813467±22022381 organism/l). The variations in phytoplankton abundance were generally non-significant between the different experimental treatments. Four divisions of phytoplankton were recorded in the experimental fish ponds; Chlorophyceae, Cyanophyceae, Bacillorophyceae and Euglinophyceae. The most abundant division in all experimental fish ponds was Chlorophyceae (42.46%); followed by Euglinophyceae (33.85%).The variations in abundance of these phytoplankton divisions were statistically significant.The phytoplankton abundance was greatly varied in each treatment ponds during different months of the experiment. Generally, it reached the highest averages during the period of September-November in all treatments, and the lowest averages were recorded during June. The results concluded that the highest stocking density (8fish/m<sup>3</sup>) with low feeding rate (2.5%) was positively correlated with best water quality and high abundance of phytoplankton in earthen ponds of <em>Oreochromis niloticus</em>.https://ejabf.journals.ekb.eg/article_22175_6aea65ef24b79979776700bccb8b3e48.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181230Food and feeding habits of the blue crab,Callinectes sapidus (Crustacea: Decapoda: Portunidae) with special reference to the gastric mill structure4174312392510.21608/ejabf.2018.23925ENRady A.Biology and Geology Dept., Faculty of Education, Ain Shams University, Cairo, Egypt.Sallam W. S.Marine Science Dept., Faculty of Science, Suez Canal University, Ismailia, Egypt.Abdou N. E. I.Biology and Geology Dept., Faculty of Education, Ain Shams University, Cairo, Egypt.El-Sayed A. A. M.Zoology Dept., Faculty of Science, Al-Azhar University, Cairo, EgyptJournal Article20181212Stomach contents of 161 specimens of <em>Callinectes sapidus</em>(Crustacea: Decapoda: Portunidae)from Bardawil Lagoon were examined from November 2016 to July 2017. The percentage point (% P) and percentage frequency of occurrence (% F) methods were used for stomach contents analysis. Out of the 161 individuals 75 (46.58 %) had empty stomach. Mollusca, Crustacea and Pisces dominated the diet of this species. According to points method Mollusca, Crustacea and Pisces scored 44.83%, 18.1% and 16.24% points in malesand 47.2%, 21.62% and 11.03% points in females, respectively. Consumed tissues of other benthic invertebrates were also present in verylow ratios, while algal fragments were occurred in low quantitiesin few stomachs. Sediments were also detected in considerable ratios. Results of the present study suggest that the blue crab <em>C. sapidus</em>is a carnivore. The remarkable seasonal fluctuations in the diet composition and stomach fullness of this species during the present studywere detected For understanding the mechanism of feeding, gastric mill structure was morphologically investigated and all its seven articulated ossicles were examined and photographically illustrated.https://ejabf.journals.ekb.eg/article_23925_e5abc1c2f2cb09d2d06382ee4e6f68a5.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181230Influence of several dietary protein and lipid levels on nutritional parameters and liver functions of Solea aegyptiaca juveniles4334462392610.21608/ejabf.2018.23926ENAbdel-Moniem M.YonesFish Nutrition Lab., Aquaculture Division, National Institute of Oceanography and Fisheries (NIOF), Cairo, EgyptHani M. NazmiFish Nutrition Lab., Aquaculture Division, National Institute of Oceanography and Fisheries (NIOF), Cairo, EgyptAtallah A. MetwalliFish Nutrition Lab., Aquaculture Division, National Institute of Oceanography and Fisheries (NIOF), Cairo, EgyptJournal Article20181118A feeding trial was designed to assess the effects of dietary protein and lipid content on growth, feed utilization efficiency, and muscle proximate composition of <em>Solea aegyptiaca</em> juveniles. Eight diets were formulated (4 protein levels; 40, 45, 50& 55% at 2 lipid levels; 8, 12%). The results showed that growth performance in terms of weight gain, specific growth rate, condition factor, feed conversion ratio, protein efficiency ratio and net protein utilization were much higher for fish group fed on a diet contained 45% protein at 12% lipid than the rest of experimental groups. The feed intake of experimental fish tended to reduce with rising dietary lipid levels. No significant differences in <em>Solea</em> <em>aegyptiaca</em> for digestibility coefficient and body chemical composition were observed. The body content of lipid and liver enzymes (alanine aminotransferase and aspartate aminotransferase) activities were positively correlated with dietary lipid levels. The present results pointed to the diet containing 45%protein and 12% lipid with 22.5 MJ/kg<sup>-1</sup> had a protein-sparing effect and was optimal for <em>Solea aegyptiaca</em> juveniles.https://ejabf.journals.ekb.eg/article_23926_89596a6b37e79d95cb7abd4b56646893.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190120Use of humic acid and Yucca extract as a benefactor on water quality and their impact on some hematological and histological parameters of Oreochromis niloticus4474602436110.21608/ejabf.2019.24361ENReham A. A.Mounes H. A. M.Ahmed K. M.Journal Article20181101Adding natural product such as Humic acid (Humabol) and Yucca schidigera extract to enhance water quality under scarce water exchange regiment; has numerus effects on both fish environment and health. Yucca showed significant reduction on NH<sub>3</sub> and NO<sub>2</sub> levels, while humic acid showed significant reduction on orthophosphate and total phosphorus compared to negative control; in addition to maintaining acceptable growth rate under designed experimental conditions. Furthermore, the hematological study showed that, Yucca and humic had positive effect on Red cell indices values such as, HCT, MCV, MCH, MCHC as well as WBCs total count and the proportion of the NEU, MONO, ESO and LYM of the treated treatments. Also, the histological study of gills, liver, stomach and intestine highlighted the tissue protective effect manifested by Yucca and humic.https://ejabf.journals.ekb.eg/article_24361_a544eb21969f92d1238afe9cef7a1d3f.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20180118Length-weight relationship, condition factor and reproductive biology of the Thin-lipped grey mullet, Liza ramada (Risso, 1826) in Bardawil Lagoon, North Sinai, Egypt.4614712525110.21608/ejabf.2018.25251ENAttia A. O. El- AiattKariman A. Sh. ShalloofJournal Article20181128This paper concentrates on thereproductive biology of the Thin-lipped grey mullet, <em>Liza ramada</em>. 1567 individuals were obtained monthly from the different landing sites of the Bardawil lagoon, North Sinai, Egypt during the fishing season from May to December, 2017. The length-weight relation was found as 0.0063 L <sup>3.0727</sup> , 0.0087 L<sup>2.98</sup> and 0.0095 L <sup>2.9505</sup> for males , females and combined sexes respectivly. Monthly condition factor values were generally low in September, and the highest values were recorded in October-December. The reproduction period (expressed by Gonado Somatic Index GSI) was determined to be in November and December.Overall ratio of males to females (M: F) was 1: 1.28. The recorded lengths at first maturity (L<sub>m</sub>) was determined as L<sub>m</sub> = 27.6 and 28.9 cm for males and females respectively . The absolute fecundity increased with total length and described by the power equation F= 27.958 L <sup>3.0135</sup> (R<sup>2 </sup>= 0.9458). The relative fecundity gradually increased from 11661.8 to 36098.3 eggs per cm. To protect <em>Liza ramada</em>in Bardawil Lagoon from exploitation, it is recommonded to ban the use of gears of illegal mesh sizes and other destructive fishing methods to permit the females to breed , grow, and recruit into the fisheries ground. Length at first maturity( L<sub>m</sub>) must be increased by widening the net mesh size to catch <em>L. ramada</em> of lengths greater than 29.0 cm. <strong> </strong>https://ejabf.journals.ekb.eg/article_25251_47e1ae88b7e226ec4cb117481f3b7c64.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181230Nutritional value and health quotient of algae collected from Egyptian coast, Alexandria4194292638510.21608/ejabf.2018.26385ENDalia M. S. A. SalemAmany El SikailyAmal E.A. Abou-talebJournal Article20180922Algal species have considerable benefits to human, because of their great nutritional value. Thus, the nutrient content (essential minerals and trace elements) and the biochemical composition (proximate content) of macroalgae (green, brown and red algae) were investigated. The results indicated the existance of high concentrations of the essential minerals as Na, K, Ca and Mg. Brown algae gave high Na and K values followed by red and green algae. While, Ca and Mg were distributed in high concentrations in red algae followed by brown and green algae. On the other hand, the essential trace elements (Fe, Cu, Zn and Mn) followed as Fe > Mn > Zn > Cu. Proximate content of algae (carbohydrates and proteins) in the three types of algae recorded as follow, for carbohydrates red algae > green algae > brown algae. Meanwhile, proteins concentration followed as red algae > brown algae > green algae. Finally, ion quotients are between 0.208 and 2.019 for green algae species, between 0 .722 and 2.087 for brown algae and between 0.458 and 2.433 for red algae. The nutrient content of studied stations water was investigated as well. From the concentration values of essential minerals in studied stations water, it was found that, Agamy station has the smallest values of Na (10.560 g/l), K (0.463 g/l), Ca (0.473 g/l) and Mg (1.573 g/l). The essential trace elements in stations water followed as Fe > Cu > Zn > Mn. The highest values of Fe (565.500 ϻg/l), Zn (20.756 ϻg/l) and Mn (4.803 ϻg/l) were recorded at Abu Quir station while, Cu measured the highest value (28.260 ϻg/l) at Agamy station.https://ejabf.journals.ekb.eg/article_26385_ed197e81b2f655bc850b6e6fd9ce711c.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190115Alpheus lobidens De Haan, 1849 (Decapoda, Alpheidae) as a new record to the Egyptian Mediterranean Coast4733822417910.21608/ejabf.2019.24179ENRasha Hamdy0000-0003-3100-8104Mohamed DorghamJournal Article20181124<em>Alpheus lobidens</em> is one of the widely distributed snapping shrimps at different depths (up to 25m) on soft and hard bottoms of the warm coastal habitats.Ithas received great attention because it was previously misidentified and described as <em>Alpheus crassimanus</em>. The present study recorded <em>A. lobidens</em> for the first time in the Eastern Harbour of Alexandria on the Egyptian Mediterranean Coast in association with a recently described bryozoan species, <em>Calyptotheca alexandriensis</em>. The seasonal abundance, biometric parameters and the females' fecundity of <em>A. lobidens </em>in the Eastern Harbour were followed during the present study. A total of 76 individuals were collected, with the highestcount during spring and the lowest in autumn. The biometric measurements showed that <em>A. lobidens </em>had lengths within the range of 9 - 32 mm and weight of 3 – 691 mg, for different maturity stages. In the meantime, Ovigerous females formed the highest percent of the population count during summer.https://ejabf.journals.ekb.eg/article_24179_a139c8af03da1702fec92caaf99fcc3e.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190101Evaluation of Water Quality and Plankton for Mahmoudia Canal in Northern West of Egypt4614742638410.21608/ejabf.2019.26384ENMohamed M. M. El-FekyAhmed E. AlprolAhmed M. M. HeneashHamdy A. Abo-TalebMohsen Y. OmerJournal Article20181020Water quality describes the condition of the water, including chemical, physical, and biological characteristics, usually with regarding to its suitability for a particular purpose. The present study describes the environmental conditions of Mahmoudia canal which are the main water source for Alexandria and Behiera governorates. Water samples were collected from ten sites along the Mahmoudia canal in the year 2018 for study the seasonal variations of the water quality parameters (physicoche-mical and biological parameters). The measured physicochemical parameters include; (Turbidity, Temperature, pH, Electrical Conductivity, Total Dissolved Solids, Total Suspended Solids, Total alkalinity, Total Hardness, Nitrate, Nitrite, Ammonia, Sulfate, Phosphate, Dissolved Oxygen, Biological Oxygen Demand, Chemical Oxygen Demand, Chlorinated pesticides and some heavy metals). Phytoplankton and Zooplankton were examined as biological parameters. The values of average water quality index (WQI) of Mahmoudia canal ranged from 75.01 (poor water quality) to 132.57 (very poor water quality). According to the results of Cluster analysis, six statistically significant clusters were formed. Three groups of phytoplankton were recorded which, the annual maximum density of phytoplankton recorded in Town of Abou Hommos, while the lowest recorded in site Zawyet Ghazal Village. The highest density registered in winter (34602 U/ml), while the lowest density recorded in summer (8316 U/ml). In addition to six zooplankton groups were quantified in the analysis of the samples from ten sites, the highest density listed in summer (60000 ind./m<sup>3</sup>) ,although the lowest listed in winter (32000 ind./m<sup>3</sup>).https://ejabf.journals.ekb.eg/article_26384_12221b979fe27b97b053ba08695660ff.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181220Record and distribution of puffer fish, Takifugu oblongus (Bloch, 1786) (Actinopterygii: Tetraodontidnae) from Tapi River, Gujarat.4014043201010.21608/ejabf.2018.32010ENThakkar NevyaSarma Kangkan JyotiTatu KetanKamboj Ravi D.Mankodi PradeepJournal Article20180724<em>Takifugu oblongus </em>(Bloch, 1786) (Actinopterygii: Tetraodontidae), is a puffer fish found in shallow coastal waters has a native distribution in entire Indo-West Pacific region (Froese and Pauly, 2014). This species is observed in shallow coastal ecosystems such as estuaries, mangroves, coral reefs and sand and gravel sediments, including the soft bottom areas of inshore and offshore regions. <em>Takifugu oblongus</em> is one of the widely distributed species among the genus <em>Takigufu</em> but no report or publication has been made so far from the western coast of India. This paper reports the record of the fish from Magdalla along the course of Tapi River, Surat, Gujarat.https://ejabf.journals.ekb.eg/article_32010_68e31cf0334aecb8114d82f160c78550.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190101Community structure of Echinoderms in littoral zone of the Red Sea Coast of Egypt4834982681410.21608/ejabf.2019.26814ENAldoushy Mahdy0000-0002-9272-0843Hamdy A. OmarSaif A. M. NasserKhaleid F. Abd El-WakeilAhmad H. Obuid-AllahJournal Article20181202The present work aimed to investigate Echinoderm communities in the Red Sea Coast of Egypt. A survey on Echinoderms done during the period between February 2016 to August 2017. Fourteen sites (42 locations) were selected to represent all Egyptian Red Sea habitats (Seagrass, mangrove, coral reef, rocky, sandy and muddy shore). A total of 33 species of echinoderms belonging to 5 classes, 12 orders and 18 families were recorded. The study revealed that the Eudominant species in this survey were:<em>Ophiocomascolopendrina</em>, <em>Diadema setosum, Echinometra mathaei</em>and <em>Holothuria atra</em> while the Dominant species included <em>Linckia multifora</em>, <em>Ophiolepis cincta</em> and <em>Tripneustes gratilla</em>. Echinoderms community showed different class and species composition in the investigated sites. Class Echinoidea recorded the highest percentage of species composition. Species richness of echinoderms ranged between three species in site 4 and twenty four species in site 1. Species diversity showed changes in different sites. The Shannon diversity (H') ranged from 0.06 to 2.24. Suez Gulf sites (sites 4, 5, 6 and 7) recorded the lowest values of diversity, while the highest value was recorded at Ras Mohamed site (site 3). The abundance and distribution differences in echinoderm communities were discussed. https://ejabf.journals.ekb.eg/article_26814_5c6a072918bd20bed30229aee7cd8ecf.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190128Bioremediation of Fe, Zn and Cd ions from aqueous solution using died cells of cyanobacterial mats from extreme habitat, Siwa Oasis, Egypt.5115222591410.21608/ejabf.2019.25914ENMohamed H. H. AliMohamed S. Abd ElkarimSamia A. HarounKhadija M. AttwaJournal Article20190105The ability of microbial cyanobacterial mats naturally grown in the extreme habitat in western desert, for removing heavy metals from its aqueous solution were studied. SEM, EDX, FTIR and surface area examinations of well fine dry dead cell of two different mats were carried out to demonstrate the physical characterizations of the biosorbent surface. The results showed that the mats characterized by high surface area, porous diameter and cracks. The dry dead cells of two mats were used to remove Zn<sup>+2</sup>, Fe<sup>+2</sup> and Cd<sup>+2</sup> ions from its aqueous solution under specific conditions; different pH, biosorbent dosage, contacts time and initial metals concentrations. The optimum removal efficiency of metals reached at pH = 6, 150 min contact time and 2.5 g/L biosrbent dose. Isotherms studies showed that Langmuir and Freundlich adsorption isotherm models were well fitted for the adsorption process. The removal of studied metals ions following the order of Zn<sup>2+</sup> > Fe<sup>2+</sup> > Cd<sup>2+</sup> according to values of maximum biosorption capacities (q<sub>max</sub>), Langmuir constant (b), separation factor (R<sub>L</sub>) and Freundlich intensity parameter (1/n). https://ejabf.journals.ekb.eg/article_25914_c5b530bb02160bf7b0ad63c2cdb1a088.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190101The impact of maritime activities on the leachable heavy metals in the seafloor sediments of Port Tawfiq and Tersana harbours, Gulf of Suez, Egypt5235362593010.21608/ejabf.2019.25930ENAhmad R. ElgendyMohamed A. HassaanFarouk A. SolimanMahmoud A. DarJournal Article20181204The bulk sediment samples and the finest sediment group (FSG) including (Ø3, Ø4 and <Ø4) were used to estimate the leachable forms of Fe, Mn, Zn, Cu, Ni, Pb, Co and Cd at Port Tawfiq and Tersana harbours in the Suez Bay at the northern tip of Suez Gulf using flame atomic absorption spectrophotometer (AAS). FSG was representing the essential constituents of the seafloor sediments with averages of 71.68% from the total sediments at Port Tawfiq Harbour and 69.47% at Tersana Harbour indicating the deposition under calm conditions. Leachable Fe was the metal carrier for the different heavy metals, the average leachable forms of Fe, Mn and Pb in FSG (53382.81, 60048.81 µg/g), (625.68, 662.54 µg/g) and (55.191, 67.23 µg/g) about two folds in the bulk sediment (26397.11, 27761.07 µg/g), (219.05, 224.22 µg/g) and (26.62, 30.47 µg/g) at both Port Tawfiq and Tersana harours respectively. Zn in FSG (594.40 µg/g) about two folds in the bulk sediments (236.34 µg/g) at Port Tawfiq Harbour, meanwhile at Tersana Harbour Zn in FSG (968.58 µg/g) about four folds in the bulk sediments (265.25 µg/g) respectively. The average leachable of Cu at Port Tawfiq Harbour in FSG (303.50 µg/g) recorded about three folds in the bulk sediments (98.01 µg/g), while at Tersana Harbour (1020.33 µg/g) recorded about five folds in the bulk sediments (98.01 µg/g). At Port Tawfiq Harbour in the FSG, the average leachable Ni (211.47 µg/g) exceeds more than four folds in the bulk sediments (50.13), meanwhile at Tersana Harbour in FSG (507.75 µg/g) about eight folds in the bulk sediments (62.08 µg/g). In the same Manner, the averages of leachable Co in FSG were about one time and half in the bulk sediments at the two studied harbours.https://ejabf.journals.ekb.eg/article_25930_012674a093ad1455928a946c0f154299.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190121Potential applications of some moderate halophilic bacteria5375502533710.21608/ejabf.2019.25337ENMohamedin, A. H.Mowafy, A.M.Elsayed, A.A.Ghanim, S. O.Journal Article20181111The characteristics of halophiles made them a target for employing in several industrial processes. In this study, four moderate halophilic bacteria isolates have been isolated from the northern coast of Mediterranean sea in Egypt. These isolates have been identified according to their 16S rRNA sequence analysis and submission on Genbank-NCBI as <em>Piscibacillus</em><em>Ish</em> and <em>Piscibacillus Pink </em>with close relevance to <em>Piscibacillus halophilus</em>, <em>Bacillus Cs</em> with close relevance to <em>Bacillus aquimaris and </em><em>Halomonas Cb</em> with close relevance to <em>Halomonas merediana. </em>The isolates <em>Piscibacillus Ish</em> and <em>Halomonas Cb</em> showed remarkable potential to produce lipase in addition to their ability to degrade the used engine oil. The isolate <em>Piscibacillus Ish</em> showed the higher growth on the oil and the lower weight of residual oil than the isolate <em>Halomonas Cb</em> and the GC-MS analysis of the digested oil sample showed that the amount of certain hydrocarbons such as C<sub>19</sub>, C<sub>21</sub>, C<sub>27</sub>, C<sub>28</sub> and C<sub>39</sub> have been reduced drastically on day 20 by a percentage of 71.83%, 78.67%, 62.21%, 74.60% and 91.95% respectively.The disappearance of hydrocarbon fractions; C<sub>7</sub>, C<sub>15</sub> and C<sub>35</sub> on day 20 suggests that these may be saturated linear alkanes, so they are easily degraded or fragmented into shorter fractions. All the isolates also were capable of degrading an industrial textile dye called Indigo dye with different degrees and the highest isolate was <em>Halomonas Cb</em>. The obtained results in this study indicated the ability of the studied moderate halophile <em>Piscibacillus Ish</em> to be used in petroleum and used engine oil degradation and the ability of the studied moderate halophile <em>Halomonas Cb</em> to be used in bioremediation of effluents produced from textile industries contaminated with Indigo dye. https://ejabf.journals.ekb.eg/article_25337_813c688cd42569e0bc951f46c6e12799.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20190115Molecular detection of Entamoeba histolytica in fresh vegetables and irrigation5515612475610.21608/ejabf.2019.24756ENFatma El-zahraa R. SalehMahmoud A. GadAmeen A. AshourMohammad I. SolimanWaled M. El-SenousyAhmad Z. Al-HerrawyJournal Article20181211<em>Entamoeba histolytica</em> (<em>E. histolytica</em>) is the causative agent of human amebiasis. As <em>E. histolytica</em> remains an important reason of morbidity and mortality in developing countries, it can cause up to 100,000 deaths/year worldwide. In this study, a survey was carried out on contamination of some common freshly eaten vegetables and their irrigation water with <em>Entamoeba histolytica</em>. Fresh vegetable samples were collected from public markets and agriculture field in Nahia and Saft areas, Giza, Egypt. A total of 255 vegetable and irrigation water samples were separately examined by PCR technique using <em>Entamoeba histolytica </em>species specific primers. The results showed that <em>Entamoeba histolytica</em> was found in 6.4% (7/110) and 3.7% (4/109) of field and market vegetable samples, respectively<em>. Entamoeba histolytica</em> was detected in 5.6% of 36 irrigation water samples collected from the same agriculture fields. A seasonal pattern of the presence of<em> Entamoeba histolytica</em> was observed with a high prevalence during cool seasons. In conclusion, contamination of freshly eaten vegetables may represent a risk to the health of consumers. Dill is the most contaminated vegetable by <em>Entamoeba histolytica.</em>https://ejabf.journals.ekb.eg/article_24756_0dff0d5e3baca8d03532fca7025fba3e.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181210Effect of Environmental Pollution on Gonads Histology of the Nile Tilapia, Oreochromis niloticus from Lake Manzala, Egypt5635722800510.21608/ejabf.2018.28005ENHala A. A. MansourMohamed A. H. El-kadyAli H. Abu AlmaatyAmal M. RamadanJournal Article20181101Lake Manzala suffers from severe pollution of complex composition from different sources of industrial, agricultural and sewage facilities from the surrounded governorates. The objective of this research was aiming to study the effects of some environmental polluted effluents at five locations of Lake Manzala on the gonads of <em>Oreochromis niloticus</em>. The histological study of testes of <em>O. niloticus</em> showed decreased number of seminiferous tubules with some degenerative changes. They appeared empty from different spermatogenic cells (primary and secondary spermatocytes). Moreover the interstitial cells were collapsed and appeared in all seminiferous tubules. The ovaries presents a deformation from their ideal shapes, thickening and detaching of the membrane of all stages of oocytes and two types of non-bursting atresia in oocytes were markedly appeared. We recommended improving water quality control and the environmental monitoring is essential at Lake Manzala.https://ejabf.journals.ekb.eg/article_28005_9bf90add72bf87117a38b105d83241d7.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181230The possibility to overcome of fish protein gap in Egypt5875953402610.21608/ejabf.2018.34026ENSaber M.M.Ahmed, M. KhIbrahim, S. M.Journal Article20181119Fish is one of the main resources of relatively cheap animal protein and Egyptian national income. Although Egypt has a large aquatic area but there is a fish protein gap, where the average per capita is lower compared to some developed countries. Therefore, the current study investigate the main reasons caused this gap during periods 1995-2015. Analysis of the factors associated with this gap and forecasting of its future status, the linear regression and Box and Jenkins method were used. The data showed that the cash value of fish recorded 23.4 billion pounds (19.6% of animal production) during 2015 (GAFRD, 2016). The average per capita was 18.2 kg\year compared with 70 kg in Japan, 35.4 in East Asia, 33.4 in Southeast Asia, and 35.1 in China during the same period. The annual growth rate of fish produced in Egypt was 3%, while it was 4.2% of consumption rate. The variation between production and consumption (276.3 thousand tones) led to a shortage in the self-sufficiency. In conclusion, this study recommends a future strategy for fish production to optimal exploit of all aquatic resources to reach self-sufficiency, processing and exporting.https://ejabf.journals.ekb.eg/article_34026_5026fad94631ac9de6c2c8e607d02531.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181230Temporal succession following flash flooding in Damietta Branch, River Nile, Egypt.5976147078010.21608/ejabf.2018.70780ENMohamed Abdel-MeguidKhadra AhmedHany Abdel-GawadJournal Article20181121Extreme weather is probably a consequence of climate change, especially periods of wet and dry spells. Extreme weather naturally impact riverine ecosystem. Therefore, comprehensive investigations of ecological processes during and after extreme events of October 2016 were carried out at Delta Barrage, Damietta Branch of the River Nile. Ten surface water samples were collected and analysed for eight physio-chemical parameters, namely temperature, pH, turbidity, dissolved oxygen, electrical conductivity, nitrate, oxidation reduction potential and crude oil. In addition, planktonic parameters were investigated. The present result showed that water quality during the flooding periods statistically changed when it was compared with pre-flood period. Moreover, the populations of both phytoplankton and zooplankton were significantly decreased in response of flash flooding. However, after five days of post flooding, a recovery has been statistically detected for some organisms.https://ejabf.journals.ekb.eg/article_70780_e97b5b00d3f49e2e85d02a0de4c69ddf.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-6131225 (Special Issue)20181230Molecular detection of three gastroenteritis viruses in an urban sewage treatment plant and river water in Egypt6156276027810.21608/ejabf.2018.60278ENMohamed N.F. ShaheenSahar E. Abd El-DaimNehal I. AhmedElmahdy M. ElmahdyJournal Article20181122<span>In this study, the prevalence of astrovirus (AstV), norovirus (NoV), and group C rotaviruses (RVC) were described in urban sewage, sewage sludge, river water, and sediment samples from Egypt. AstV, NoV, and RVC were detected in 58.3%, 33.3%, and 25% of the raw sewage samples; in 33.3%, 25%, and 16.6% of the treated sewage samples; and in 66.6%, 16.6%, and 8.3% of the sludge samples, respectively. On the other hand, AstV, NoV, and RVC were detected in 25%, 16.6%, and 8.3% of the river water samples and in 16.6%, 8.3%, and 0% of the river sediment samples, respectively. The study revealed that AstV genogroup B and NoV genogroup I are the most frequent genotypes in the Egyptian environment. Furthermore, the peak prevalence of AstV and RVC in river water and raw sewage samples was found in winter months however there was no clear seasonality for NoV spread</span><em><span>.</span></em><span> This study support the importance of considering viral markers to ensure the quality of water and the utilization of these markers as additional tests for the characterization of water contamination.</span>https://ejabf.journals.ekb.eg/article_60278_23e8637ca9507737ec260dfe83a6911b.pdf