The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Potential role of Ceratophyllum demersum in bioaccumulation and tolerance of some heavy metals
1
12
EN
Kadria M. A.
Mahmoud
Hassnaa A.
Mahmoud
Sara S. M.
Sayed
10.21608/ejabf.2018.9738
Contamination of aquatic ecosystems by heavy metals is considered one of the most major threats of the aquatic life. So, the present work aims to investigate the accumulation and tolerance of <em>Ceratophyllum demersum</em> plant to different concentrations of Pb<sup>+2</sup>, Al<sup>+3</sup> and Cd<sup>+2</sup> during four treatment periods for 16 days. Results indicated that the highest tolerance percentage of <em>C. demersum</em> was (96.26 %) of Pb at 10 mg L<sup>-1 </sup>after 4 days; whereas the highest tolerance percentage values were (95.89 % and 80.84 %) at 3 mgL<sup>-1</sup> of Al and at 0.1 mg L<sup>-1</sup> of Cd after 8 days, respectively. <em>C. demersum</em> accumulated the highest amount of Pb (17029.19 μg g<sup>-1</sup>) at 50 mg L<sup>-1</sup> after 12 days, followed by Al amount which was (2893.04 μg g<sup>-1</sup>) at 9 mg L<sup>-1</sup> after 8 days and the highest accumulation value of Cd was (521.28 μg g<sup>-1</sup>) at 0.5 mg L<sup>-1</sup> after 4 days. Metal accumulation in <em>C. demersum</em> showed at a descending order as follow; Pb >Al >Cd and it was significantly increased with increasing the concentration and treatment period. In addition, results showed that the maximum BCF value of Cd in <em>C. demersum</em> at low concentration was (1295.37) after 12 days of the treatment. Therefore,<em> C. demersum</em> is considered a hyperaccumulator species for cadmium.
Ceratophyllum demersum,Heavy metals,bioaccumulation,Bioconcentration factor,Lead,cadmium,tolerance
https://ejabf.journals.ekb.eg/article_9738.html
https://ejabf.journals.ekb.eg/article_9738_3ff6017354929186cbce30201b892832.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Ecological Assessment of the River Nile Status around Gizert El-Warrak using Phytoplankton and Macroinvertebrates Assemblages
13
24
EN
Kadria M. A.
Mahmoud
Sara S. M.
Sayed
Mohamed R.
Habib
m_ramadanhabib@yahoo.com
10.21608/ejabf.2018.10108
Biological assessment is considered a useful tool for assessing the ecological status of the aquatic ecosystems. Therefore, the goal of the present study was to use phytoplankton and macroinvertebrates as biological tools for ecological assessment of the River Nile around Gizert El-Warrak. A total of 33 phytoplankton species were recorded at the studied stations; these include 16 species of Chlorophyta<em>,</em> 5 Cyanophyta and 12 Bacillariophyta species. All investigated stations were characterized by high organic pollution according to Palmer′s index. Trophic state index showed a hyper-eutrophic status in stations S1, S2, S4, S6 and S8 and an eutrophic status in stations S3, S5 and S7.Gastropoda and Oligochaeta were the most dominant of macroinvertebrates taxa recorded 50.8 and 24.6% of all biota, respectively. Diversity Index (H′) ranged between (1.14 - 2), which indicated that the structure of macroinvertebrate habitats was poor. Moreover, Evenness Index (J) ranged between (0.016 – 0.043), which indicated that individuals were not distributed equally. The values of biotic index (HBI) depending on macroinvertebrates categories showed that the River Nile’s water quality is fairly poor with significant organic pollution.
River Nile,Gizert El-Warrak,phytoplankton,Macroinvertebrates,Bioassessment,Biotic index,Palmer′s index
https://ejabf.journals.ekb.eg/article_10108.html
https://ejabf.journals.ekb.eg/article_10108_7accf2185f82a9a011dcdf5f9cc2b61a.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Trends in genetic divergence of Biomphalaria alexandrina snails and Oreochromis niloticus fish in response to water quality of Lake Burullus, Egypt.
25
47
EN
Hanaa M. M.
El-Khayat
Sohair
Abd-Elkawy
Mervat A.
Ahmed
Nouran A.
Abou- Ouf
Wafaa A.
Mohammed
10.21608/ejabf.2018.11113
Water quality of Lake Burullus was investigated through evaluating their impact on health and genetic alterations of native Lake Burullus <em>Biomphalaria alexandrina </em>snails and <em>Oreochromis niloticus</em> fish samples and non-native ones exposed in simulating experiments for six weeks to lake-water/sediment from three lake sites; El-Hamoul, Mottobus and Sidi-Salem. El-Hamoul and Sidi-Salem sites showed high levels of total dissolved solids and conductivity, while recorded high levels of Zn and Cd in their simulating experiments. Native snails' tissues showed higher Cu bioaccumulation, while non-native showed higher levels of Zn and Cd bioaccumulation. Most non-native exposed fish in the three simulating experiments showed higher bioaccumulation in all organs than native fish from Lake Burullus. Snail hemolymph showed significant increase in small granulocytes and decrease in hyalinocytes, while fish blood showed highly significant increase in WBCs of native fish collected from El-Hamoul and Sidi-Salem sites and non-significant decrease in Hb and RBCs in all samples. Genetic alterations were investigated by ISSR-PCR technique demonstrated 33 amplified bands of which 30 % were polymorphic among snail samples, while generated 26 amplified bands among fish samples of which 40% were polymorphic. Cluster analysis using dendrograms based on genetic similarity matrices was constructed between each of snail and fish samples showed different degrees of similarities and divergent as showed it cluster between samples of control, lake and non-native Mottobus indicating lower pollution;while non-native El-Hamoul sample was the most divergent followed by non-native Sidi-Salem sample indicating higher pollution level. So, heavy metals bioaccumulation, hematological, and molecular analysis indicate that water quality of Lake Burullus has negative effects on snail and fish samples graded from Sidi-Salem > El-Hamoul >Mottobus samples.
Biomphalaria alexandrina,Oreochromis niloticus,Lake Burullus,genetic divergence,Polymorphism,pollution
https://ejabf.journals.ekb.eg/article_11113.html
https://ejabf.journals.ekb.eg/article_11113_a3893faa7fe9959490e293c417f7c692.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Assessment of polycyclic aromatic hydrocarbons in surface sediments and some fish species from the Gulf of Suez, Egypt.
49
59
EN
Alaa M.
Younis
Yosry A.
Soliman
Eman M.
Elkady
Mohamed H.
El-Naggar
10.21608/ejabf.2018.12472
The present study evaluated the origin and distribution of sixteen polycyclic aromatic hydrocarbons (PAHs) included by the <em>United States Environmental Protection Agency </em>(US EPA) within the priority pollutants were considered in the surface sediment as well as eleven fish species of Suez Gulf, Egypt. Sediment samples were collected at 0.5 and 10 meter depth from ten stations in addition to eleven fish species (<em>Liza subviridis, Parupeneus forsskali, Upeneus japonicus, Lethrinus nebulosus, Stephanolepis diaspros, Epinephelus areolatu, Sphyraena chrysotaenia, Pomadasys stridens, Trachurus indicus, Saurida undosquamis, Engyprospon </em>sp.). The concentrations of PAHs were measured by gas chromatography (GC) equipped with FID detector.The total concentration ∑16 PAHs ranged from1667.02 to 2671.27ng/g with the highest levels recorded at stations IV, VII and X (Ras Gharib, AionMousa and Abu Zanima). While the total concentration ∑16 PAHs in collected fish species ranged from 621 to 4207 ng/g wet weight with the highest ∑16 PAHs found in species <em>Saurida undosquamis</em>, while the lowest found in the species <em>Stephanolepis diaspros.</em> The high molecular weight PAHs (HPAHs) were predominant if compared with the low molecular weight PAHs (LPAHs). With LPAHs/HPAHs values less than one for all studied species indicating anthropogenic origin of PAHs in fish. Furthermore, the study of the PAHs diagnostic ratios suggests the predominance of pyrolytic origin for the studied PAHs.
Hydrocarbons,PAHs,Suez Gulf,sediments,fish
https://ejabf.journals.ekb.eg/article_12472.html
https://ejabf.journals.ekb.eg/article_12472_f6e6d2cd9ea6f180ce4fc4fefe00ca95.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Histopathological, Ultrastructural and Morphometric Studies on the Effect of Atorvastatin on Rat Kidney.
61
75
EN
Ashgan W.
Saber
Nagui H.
Fares
Larissa S.
Ananieva
Amal I.
Mohamed
Fairoze K.
Ibrahim
10.21608/ejabf.2018.10509
Atorvastatin reduces the levels of "bad" cholesterol (LDL) and triglycerides in the blood, while increasing levels of "good" cholesterol (HDL). Accordingly, the aim of the present study was to determine the potential toxicity of the therapeutic doses of atorvastatin in male albino rats. A total of fifteen adult male albino rats were divided randomly into three groups.
The control group (group 1) did not receive any medication, while group II received atorvastatin (10 mg/kg/day for four weeks). Group III received atorvastatin (10 mg/kg/day for four weeks) then left for one month after the last dose for recovery from the drug. Kidney biopsies were taken from each rat for histopathological and ultrastructural examinations. In comparison with respective control rats, the results showed slight effects of atorvastatin on the kidney of rats, which was in the form of vacuolization, degeneration and cloudy swelling of epithelial cells in the tubules with the formation of cell debris inside the lumen of proximal convoluted tubules and diminish in urinary space of glomeruli.
These effects were partially irreversible in recovery group. Accordingly, it is highly recommended that patients who suffer from hypercholesterolemia may be advised to feed on hypocholestrolaemic food regime before prescribing statins. If patient need to prescribe a hypocholestrolaemic drugs, statin should be taken in short repeated duration with at least one month between each period to avoid side effects and make a follow up every month for laboratory measurement of kidney functions.
Atorvastatin,hyperlipidemia,Cholesterol,triglycerides,kidney
https://ejabf.journals.ekb.eg/article_10509.html
https://ejabf.journals.ekb.eg/article_10509_af26fdc54682d8babdcb9a856f797b59.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Evaluation of some leachable heavy metals in the Seafloor sediments of the two navigation Harbours El Zaitiya and Adabiya, Gulf of Suez, Egypt
77
92
EN
Ahmed R.
Elgendy
Farouk A.
Soliman
Mahmoud Abdel-Radi
Dar
Ahmed R.
Hassan
Lamiaa I.
Mohamedein
Mohamed A.
Hassaan
10.21608/ejabf.2018.12299
The finest sediment group (FSG) including (Ø<sub>3</sub>, Ø<sub>4</sub> and <Ø<sub>4</sub>) and the bulk sediment samples were used to estimate the leachable forms of Fe, Mn, Zn, Cu, Ni, Pb, Co and Cd at Adabiya and El Zaitiya harbours in the Suez Bay at the northern tip of Suez Gulf using flame atomic absorption spectrophotometer (AAS). FSG was representing the essential constituents of the seafloor sediments with averages of 70.62% from the total sediments at El-Zaityia Harbour and 70.38% at Adabiya Harbour indicating the deposition under calm oceanographic conditions. Fe was the metal carrier for the different heavy metals, the average leachable forms of Fe and Zn in the FSG (50924.94; 377.61 µg/g) about two folds in the bulk sediment (27153.9; 180.47µg/g) at El Zaityia Harbour and about three folds in the FSG (43965.42; 456.94 µg/g) which more than the bulk sediments (17777.75; 134.62 µg/g) at Adabiya Harbour. In the same Matter, the averages of leachable forms of Mn, Cu, Ni and Pb in the FSG recorded about three folds (532.15; 195.38, 54.60, 145.94 µg/g) at El Zaitiya Harbour and (476.88; 109.42, 47.35, 67.11 µg/g) Adabiya Harbour than the recorded averages in the bulk sediment (182.43; 67.01, 19.01, 62.80 µg/g) at El Zaitiya Harbour (155.56, 35.14, 15.78, 20.27 µg/g) and Adabiya Harbour respectively.
Heavy metals,Suez Gulf,Navigation Harbours,Fine Sediments,Bulk Sediments,pollution
https://ejabf.journals.ekb.eg/article_12299.html
https://ejabf.journals.ekb.eg/article_12299_dcf73f020afa630ad10e5a3280c54fad.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Advantageous effects of dietary butyrate on growth, immunity response, intestinal microbiota and histomorphology of European Seabass (Dicentrarchus labrax) fry
93
110
EN
Heba H.
Abdel-Mohsen
Elham A.
Wassef
Nagy M.
El-Bermawy
Nabila E.
Abdel-Meguid
Norhan E.
Saleh
Khouloud M.
Barakat
Omayma E.
Shaltout
10.21608/ejabf.2018.12055
A feeding trial was conducted to evaluate response of European seabass (ESB, <em>Dicentrarchus labrax</em>) fry to graded levels of the dietary organic salt, sodium butyrate (SB). ESB with 0.45 g were fed four experimental diets contained: 0, 0.1, 0.2 and 0.3% SB and assigned as: control (CTRL), SB1, SB2 and SB3 respectively for 12-weeks. Results showed a significant (P≤0.05) elevation of all measured growth criteria induced by SB2 and SB3 diets intake relative to CTRL diet (SB-free). Both 0.2% and 0.3% dietary SB supplementation had led to appreciable increase in protein concomitant with decrease in lipid contents of fish, as well as enhancement of fish hematological profile. All measured immunological parameters in fish serum have remarkably increased: immunoglobulin (+198%), respiratory burst activity (+78.2%), phagocytosis (+42.3%), myeloperoxidase (+42.2%), lysozyme (+10.6%) and bactericidal (42.8%) activities with the oral-ingestion of SB, particularly at 0.2% then 0.3% level in comparison to CTRL. Besides, SB can maintain a healthy balance of bacterial load in the gut through boosting beneficial bacteria and inhibiting pathogens within the distal intestine, thereby modulate and stimulate immunity response of fish. Records of intestinal sections-measurement emphasized the positive effects of SB2 diet on the intestinal lumen causing activation in the development of muscle layers-thickness, goblet cells count and villi length and width of fish. These results evidenced the advantageous effect of SB on gut-health functionality, leading to higher capacity of disease resistance and growth rate for ESB fry. In conclusion, our findings indicate that inclusion of 2g micro-encapsulated SB/Kg diet could provide a safer potent alternative to antibiotic use, to benefit health status, reinforce immunity response, modulate the distal-intestine microbiota and increase cell-proliferation in the intestinal crypts of ESB fry.
European seabass,Dicentrarchus labrax,Sodium butyrate,gut microbiota,Intestine histomorphology,immunity response
https://ejabf.journals.ekb.eg/article_12055.html
https://ejabf.journals.ekb.eg/article_12055_ccc4124e0bd8bb73bbfd25e940366a17.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Ecological and Taxonomical Characterizations of some Molluscan species in Irrigated Nile Channels, (Rayahs), Egypt
111
124
EN
Shaimaa M.
Ibrahim
Hosam E.
El Saied
Mohamed
Abd El Mordy
Magdy T.
Khalil
mtkhalil52@hotmail.com
Gamal M.
El Shabrawy
Reda E.
Bindary
10.21608/ejabf.2018.12300
Taxonomy and seasonal diversity of Nile molluscs are important to understand the ecological impact of these animals on sustainability of River Nile ecosystem dynamics. The present work aims to explore and evaluate the taxonomy and diversity of four benthic molluscan species; two gastropods and two bivalves, in three of Egyptian irrigated Nile channels (Rayahs). The specimens were collected seasonally during the period from spring 2014 to winter 2015.Taxonomy of specimens were studied from the views of morphology and 18S rRNA gene analysis. The Gastropod species -1 and -2 had 5-6 light or dark brown whorls, but the last whorl of Gastropod sp.-2 was larger than that in Gastropodsp.-1. The phylogenetic analyses of 18S rRNA gene showed that both of Gastropod species formed monophyletic cluster, which had paraphyletic lineage with species <em>Viviparus georgianus</em>, implicating two new species under the genus <em>Viviparus</em>. The shell of the Bivalve sp.-1was ovoid with light greenish brownish or yellowish color, while the Bivalvesp.-2 had light brown to grayish elongated shell. Both of Bivalve spp.-1and-2 showed 18S rRNA gene sequence identity; averages of 89% and 92%, respectively, with <em>Lampsilis cardium</em> suggesting new genera within the family Unionidae. Gastropod sp.-1 showed highest abundance, during winter in El-Rayah El-Tawfiky, while the lowest was recorded during spring in El-Rayah El-Behery. Gastropod sp.-2 was abundant during summer in El -Rayah El- Behery. The seasonal distribution of the two studied bivalves declared the highest abundance in both of El-Rayah El-Behery and El-Rayah El-Tawfiky during winter, while declined during spring in all Rayahs.
Mollusca,Ecology,Taxonomy,Morphology,18S rRNA gene,Biogeographic distribution,Nile Rayahs
https://ejabf.journals.ekb.eg/article_12300.html
https://ejabf.journals.ekb.eg/article_12300_c4cf2888a8624fa21e02fe3a42643a8a.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Molecular Identification of Grey Mullet species in the Mediterranean Sea of Egypt
125
139
EN
Ashgan
A. A.
Abou-Gabal
Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria, Egypt
Eman
M.
Abbas
0000-0002-1716-025X
Genetic and Genetic Engineering Department, National Institute of Oceanography and Fisheries, Alexandria, Egypt
emanabbas03@yahoo.com
Hala
Mahmoud Mohamed
Ali
Genetics, Faculty of Agriculture, Alexandria University.
hala.mahmod90@yahoo.com
Nagy
El-Baramawi
Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria, Egypt
Asmaa
A.
Khaled
Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria, Egypt
dr.asmaa_khaled@alexu.edu.eg
Safaa
I.
El Deeb
Genetic and Genetic Engineering Department, National Institute of Oceanography and Fisheries, Alexandria, Egypt
10.21608/ejabf.2018.13263
Mullet fish provide a valuable role in aquaculture to meet the rising demands for fish production in Egypt. The accurate estimate of species limits is a key factor in improving mullet fish for the application in evolution, conservation and management of the genetic programs. Four species of mullet fish, “<em>Mugil cephalus</em>, <em>Liza ramada</em>, <em>Liza aurata</em>, and <em>Liza saline</em>” were investigated by RAPD analysis to assess the genetic structure. Mullet fish were collected from three locations in the Mediterranean Sea (Al Maadyeah – Al Manzala – Bardawil). RAPD assay was performed using 10 decamers random primers; all the ten primers were succeeded in producing distinct bands.The obtained results revealed that the number of reproducible bands analyzed for ten primers for Al Maadyeah fishes was 181 bands in all four species, but in Bardawil and Manzala were 224 and 229 respectively. In each interspecies Mullet variation, the polymorphic amplified bands were 78.12% from it (20.98% <em>M. cephalus</em> 5.35% <em>L. aurata</em>, and 16.07 % <em>Liza ramada</em>unique bands) in Al Manzala, and 73.79% polymorphic from it (20.96% <em>M. cephalus</em>, 4.36% <em>L. aurata</em> and 12.2% <em>L. ramada </em>unique bands) in Bardawil and 66.85% polymorphic bands for Al Maadyeah from it (11.6% <em>M. cephalus</em> 8.2% <em>L. aurata</em>, 1.1% for <em>L. ramada</em> and <em>L. saline</em>unique Bands). Nei’s genetic distance between (0.503 – 0.719) in Al Manzala, (0.263 – 0.514) in Al Maadyeah and (0.271 – 0.537) in Bardawil. The RAPD technique can be introduced as an easy tool in the population genetics of marine fishes, providing supplementary information in their genetic stock structure analysis.
Mullet fish,Mediterranean Sea,RAPD,PCR,Mugilidae Family,species differentiation,phylogenetic tree
https://ejabf.journals.ekb.eg/article_13263.html
https://ejabf.journals.ekb.eg/article_13263_608d3b4316a9ad59e3de8dce74005dcb.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Histological alterations in some organs of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) produced using methyltestosterone
141
151
EN
Alaa El-Din
Sayed
0000-0001-7487-4729
Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt
alaa_h254@yahoo.com
Mahmoud
Farrag
0000-0001-6413-5172
Faculty of science, Al-Azhar University (Assiut), Egypt
m_mahrousfarrag@yahoo.com
Basem
Abdelaty
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Mohammed
Toutou
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Ola
Muhammad
Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt
10.21608/ejabf.2018.13272
17α-methyltestosterone is the most efficient and least expensive method in monosex production in Egypt. This study was carried out to investigate the effect of this hormone on farmed Nile tilapia (<em>Oreochromis niloticus</em>) using some histological parameters. Fish samples were collected from four localities (Assiut; as a control and Beheira, Alexandria, Kafr El-Sheikh; as monosex populations). Histological sections in liver, kidney, and testis were prepared, stained, and examined according to histology protocol. Microscopic examination has been done for random samples showing some alterations, such as damages central vein (CV), nicrotic area (N), and hyperplasia in the hepatocytes (H) and Kuffer cells (Kc) in fish collected from Kafr El-Shiek farms. Sections of liver of tilapia from Alexandria showed hydropic degeneration (HD) in the hepatocytes (H), hyperplasia in the Kupffer cells (Kc) and blood congestion (BC) in the central vein. Moreover, sections of liver of tilapia from Beheira showed the hepatocytes (H) and the central vein (CV) with presence of melanomacrophages (M) and hyperplasia in the hepatocytes and Kupffer cells (Kc). Kidney of tilapia from monosex farms showed enlarged glomeruli (EG) and damaged renal tubules, detached epithelial cells and damaged renal tubules. Testis showed degeneration of the interstitial tissues and disorganization in the seminiferous tubules. It has been concluded that, the overdose of methyltestosterone (MT) induction affected some oranges in monosex fish.
Oreochromis niloticus,monosex,methyltestosterone,melanomacrophages,Hyperplasia
https://ejabf.journals.ekb.eg/article_13272.html
https://ejabf.journals.ekb.eg/article_13272_a2d891cb9f756f93674830ce532500bf.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Assessment of some Pesticides in Water, Sediment, Clarias gariepinus and Procambarus clarkii from two localities at Muweis canal, Zagazig, Egypt
153
166
EN
Zeinab Z. K. Khater
Zeinab Z. K. Khater
Department of Zoology, Faculty of Science, Zagazig University, Egypt
10.21608/ejabf.2018.13574
The distribution of pesticides in water, sediments, <em>Clarias gariepinus</em>, and<em> Procambarus clarkii </em>from Muweis canal at two localities was investigated to evaluate the pollution status and potential hazard in the river system. A total of 28 pesticides were analyzed using gas chromatography (GC) with electron capture detector. The concentration of pesticide residues ranged from ND (not detected) to 0.560mg/l for water samples, ND to 0.73 μg/g dw for sediment, ND to 9.653 μg/g ww for <em>C. gariepinus</em>, and ND to 6.875 μg/g ww for <em>P. clarkii</em>. High concentrations of organochlorine pesticides, specifically fenpropathrin, endosulfan ii, delta-BHC and dieldrin observed in all environmental media, are an indication of the current illegal use of banned pesticides for agricultural activities in the region. Tal-Haween water samples revealed the highest content of pesticides followed by El-Shabakat water, while the sediment of Tal-Haween observed more contaminated than that of El-Shabakat. Also, <em>C. gariepinus </em>exhibited more polluted than <em>P. clarkii</em>. Analysis of data showed significant differences between gills of <em>C. gariepinus</em>, water and sediment samples of Tal-Haween site in fenpropathrin and endosulfan ii respectively, while exhibited a highly significant difference between water and sediment samples of El-Shabakat site in aldrin. Moreover, there was a highly significant difference in correlation coefficient between water and sediment samples of El-Shabakat site in endosulfan ii and endrin. Concerning the mean concentrations of some pesticides in water and fish, the levels were higher than that recommended by the international permissible limits. It is an indication of a potential cancer hazard for the local inhabitants with lifetime consumption of pesticide-polluted fish.
Procambarus clarkia,Clarias gariepinus,Muweis canal,sediments,organochlorine,Pesticides
https://ejabf.journals.ekb.eg/article_13574.html
https://ejabf.journals.ekb.eg/article_13574_e4cd75edc26b4ee7315b11807ade2c20.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Growth response, nutrient utilization, biochemical and hematological parameters of Juvenile African catfish (clarias gariepinus) fed diet supplemented with gongronema latifolia (benth) extract.
167
179
EN
Aderolu,
Ademola zaid
Ariyo,
Taiwo Rashidat
Oke,
Adetola Ibitayo
10.21608/ejabf.2018.16562
This study investigated the effect of varying levels of <em>Gongronema latifolia</em> on growth, nutrient utilization, biochemical and hematological parameters of <em>Clarias gariepinus</em> juveniles. One hundred and five (105) juveniles were equally distributed in fifteen plastics tanks and each triplicated. Three inclusion levels of the plant extract (150mg/kg, 300mg/kg and 500mg/kg) were prepared, while the antibiotics served as negative control and the diet without any inclusion served as the positive control. The fish meal was supplemented with <em>Gongronema latifolia</em> (benth) extract and the control diets were fed to satisfaction, while the tank water was changed every other day. The weight gained and feed intakes of the fish were determined at the end of every week and the growth parameters were evaluated. Growth parameters showed that the highest value was recorded in treatment 4 (300mg) 226.07±9.96 for the final weight gained, while the lowest was observed in treatment 5(500mg) was 165.47±31.55. Supplementation of fish feed on <em>Gongronema latifolia</em> did not cause any adverse effects on biomarkers of liver toxicity and oxidative stress. Monocytes and lymphocytes concentration were significantly reduced with no alteration in red blood cell count and hemoglobin concentration. This study showed that the supplementation of <em>Clarias gariepinus</em> juvenile diet with <em>G. latifolia e</em>xtract promoted growth and weight gain in the fish stock with no toxic effect on the liver and oxidative stress.
Clarias gariepinus,fish diet,Growth performance,G. latifolia,Oxidative Stress,liver toxicity
https://ejabf.journals.ekb.eg/article_16562.html
https://ejabf.journals.ekb.eg/article_16562_ddf6ff4f4e73f1bfda40f5dbdcd73f05.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Fenton Oxidation and Fungal Remediation of Different Pollutants from Kitchener Drain, Egypt.
181
193
EN
Ahmed
S. Gebreil
Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
Muhammad
Abdul-Hady
El-Alfy
Marine Pollution Department, National Institute of Oceanography & Fisheries, Egypt
muhammad.elalfy@yahoo.com
Yasser
A. El-Amier
Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
Eman
H. Abd El-Salam
Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
10.21608/ejabf.2018.13898
Wastewater from Kitchener drain cause many effects not only on the surrounding environment but when being discharged into the Mediterranean Sea water. Using different remediation processes for heavy metals removal has important role in purification of different types of wastewaters aid in recycling process. This work is aimed to use Fenton as chemical remediation process for reduction of organic pollutants and to screen the efficiency of the fungal isolates in heavy metal removal process by generation of an immobilized fungal consortium. Parameters affecting the Fenton process as pH, dosages of Fenton reagents and temperature were taken into considerations in this research. Results showed that the optimum conditions to have high removal of organic matter from wastewater sample at the experiment were at FeSO<sub>4</sub> (0.4 gm), hydrogen peroxide H<sub>2</sub>O<sub>2</sub> (15%), at acidic medium (pH = 2) and temperature of about 40°C. It showed high removal of chemical oxygen demand COD concentration from sample with 80 to 95% removal. On the other experiment, a mixture of fungal spores was used to remove Chromium, Cadmium and Nickel ions in batch modes. In this mode, the immobilized biomass was enclosed in a hanged tea bag-like cellulose membrane to facilitate the separation of the biosorbent from the treated solutions, which is one of the main challenges in applying microbial biosorption at large scale. The chemical remediation approved the potentiality to reduce non-biodegradable components which can’t be consumed by bio-remediates i.e. fungi and bacteria. Where, all the obtained biosorbent isolates showed more significant efficiency in heavy metal removal. The most potential biosorbent fungi were identified and characterized. Finally, these remediation methods can be applied for removal of different pollutants from wastewaters.
Wastewater,Kitchener Drain,Remediation,Fenton,fungi,Batch Mode
https://ejabf.journals.ekb.eg/article_13898.html
https://ejabf.journals.ekb.eg/article_13898_7d73f416092b035c8b9450802c19164d.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Enhancement of lipid accumulation in oleaginous marine yeast; Candida sp. for biodiesel production and antimicrobial properties
195
207
EN
Sahar W.M.
Hassan
Khouloud M.
Barakat
Hassan A.H.
Ibrahim
10.21608/ejabf.2018.17098
Fatty acids, both free or as part of complex lipids, are essential components of all vital membranes. Thus, the aim of the present study is the production of fatty acids by marine yeast using some natural of low price sources. Marine <em>Candida</em> sp. isolated from SidiBishr, Mediterranean Sea, Egypt, showed the highest capabilities of lipid accumulation by 42.8% in 1.9 gl<sup>-1</sup> dry biomass. Optimization of the fermented conditions for lipid production was carried out by using one factor at time. Molasses and urea as carbon and nitrogen sources showed the highest lipid content 71% in 6 gl<sup>-1</sup> biomass at pH 5, 25<sup>o</sup>C and 20% inoculum size, where, lipid accumulation and dry biomass of marine <em>Candida </em>sp. increased by 1.7 and 3.2 fold, respectively. Esterification of the accumulative lipid was performed, resulting in 34 fatty acid methyl esters (FAMEs) detected by Gas chromatographic analysis. Biodiesel properties were estimated showing the Degree of Unsaturation (DU), 98.87; Long Chain Saturation Factor (LCSF), 13.96; Iodine Value (IV), 105.07; Saponification Value (SV), 190.34; the Cetane Number (CN), 57.43; Kinematic Viscosity (υ), 4.52; Density (ρ), 0.87 and the Higher Heating Value (HHV), 40.05. Antibacterial activity of the extracted lipid showed inhibition zone diameters ranged from 10 to 17 mm. Moreover antifungal activity against some pathogens ranged from 50 -100%.
https://ejabf.journals.ekb.eg/article_17098.html
https://ejabf.journals.ekb.eg/article_17098_a4dbb7d72f3b30f8828e06741d477be3.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Intermittent study of benthic fauna in the Eastern Harbour of Alexandria, Egypt.
209
223
EN
Rasha
Hamdy
Mohamed
Dorgham
10.21608/ejabf.2018.17099
The hard bottom benthic fauna at a fixed site in the Eastern Harbour of Alexandria, Egypt was studied during intermittent seasons from 2014 to 2018. The results recorded 26 species, belong mainly to polychaetes (11 species) and crustaceans (9 species), in addition to 2 species of mollusks and one species for each of Cnidaria, Porifera, Bryozoa and Sipuncula. The species richness varied between 9 species after the winter storm of 2014 to 21 species in spring 2015, while the numerical abundance of the total fauna fluctuated between 1007 ind./m<sup>2</sup> in spring 2017 and 3442.3 ind./m<sup>2</sup> before the winter storm of 2014. The Shannon diversity index was low (0.98 - 1.51), associated with low evenness (0.39 -0.69). Polychaetes constituted 45.2% of the total faunal count, followed by crustaceans (35.2%), and mollusks (19.4%). Five species only appeared to be responsible for 91.7% of the total count, namely the polychaetes <em>Pseudonereis anomala</em> Gravier (1899) and <em>Spirobranchus triqueter </em>Linnaeus (1758), the amphipod <em>Elasmopus pectenicrus</em> Spence Bate (1862), the isopod <em>Cirolana bovina</em> Barnard (1940), and the bivalve <em>Brachidontes pharaonis</em> P. Fischer (1870). Other species sustained relatively high counts, forming high percent of the total population at certain time, such as the polychaetes <em>Syllis schulzi </em>Hartmann-Schröder (1960), <em>Loimia medusa</em> Savigny (1822), the isopod <em>Dynamene bidentata </em>Adams (1800), and the gastropod <em>Fissurella</em> sp. The counts and relative abundance of the major groups as well as the dominant species experienced pronounced temporal variation throughout the period of study.
Polychaetes,Crustaceans,coastal fauna,Alexandria coast,Eastern Harbour
https://ejabf.journals.ekb.eg/article_17099.html
https://ejabf.journals.ekb.eg/article_17099_bd62527030991ecc4cae97312a4fe92b.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Comparing effects of feeding crystalline or coated methionine to soybean meal based diets on the Nile tilapia (Oreochromis niloticus) growth performance and protein quality
221
232
EN
Ashraf
H. Gomaa
Regional Center for Food and Feed, Agricultural Research Center, Giza, Egypt.
Mohamed
A. El-Sherbiny
Regional Center for Food and Feed, Agricultural Research Center, Giza, Egypt.
Mamdouh
T. Eassawy
Regional Center for Food and Feed, Agricultural Research Center, Giza, Egypt.
10.21608/ejabf.2018.17158
A sixty days feeding trial was conducted to investigate the influence of including different forms of dietary methionine on growth performance and plasma methionine levels in Nile tilapia diets <em>(Oreochromis niloticus).</em> One hundred and thirty two Nile tilapia fingerlings <em>(Oreochromis niloticus)</em> mono sex of mean initial body weight 24.32 ± 0.31g were randomly distributed into 12 closed system 120 liter tanks. Fish in each three tanks were fed one of four diets. 1) Control positive (C+) (fish meal as main protein source covering methionine requirement for Nile tilapia), 2) Control negative (C-) (soybean meal as primary protein source deficient in methionine), 3) soybean meal supplemented with crystalline DL methionine (CRM), or 4) soybean meal supplemented with coated methionine (COM). Before the end of the experiment by 48h., blood samples were obtained at 1.5, 3.5, 5.5, 12.5 and 24h after feed administration to measure methionine uptake in blood plasma. Results revealed that supplying fish diet free of animal protein source with coated methionine (COM) had comparable final body weight(FBW), weight gain (WG), feed conversion ratio (FCR) and specific growth rate (SGR) with the control group (P>0.05). The poorest growth performance and feed utilization was exhibited by the group fed the negative control (C-) diet. Apparent protein digestibility were significantly higher (P<0.05) in groups fed diets supplemented with either coated or crystalline methionine compared with groups fed un-supplemented diets (C+ and C-). Dorsal muscle protein and total essential amino acids (ΣEAA %) content were not significantly affected by dietary treatments. Plasma methionine concentration was significantly influenced by both protein source and methionine form, where the highest plasma methionine concentration throughout the measuring period was revealed by fish fed diet supplemented with crystalline methionine (CRM), while the lowest plasma methionine concentration resulted from group fed (C-). Both group of fish fed (C+) or (COM) had moderate plasma methionine concentration. Based on these results, it appeared that coated methionine effectively slow the release of free methionine in a way to be near the animal bounded protein. Accordingly, it could be recommended to supplement soybean- based diets with coated methionine which had shown no adverse effect on Nile tilapia growth performance and protein utilization efficiency.
methionine,crystalline,plasma,Growth performance,Nile tilapia
https://ejabf.journals.ekb.eg/article_17158.html
https://ejabf.journals.ekb.eg/article_17158_3bafcee7f3a2e5a7f5af4f9fe0fb5139.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Reproductive biology of the yellow-striped goatfish Mulloidichthys flavolineatus (Lacepède, 1801) (Perciformes: Mullidae) in the Red Sea, Egypt.
233
247
EN
Mohamed
A. Abu El-Regal
Marine Science Department, Faculty of Science, Port Said University, Port Said, Egypt
10.21608/ejabf.2018.17159
The Red Sea population of the yellow-striped goatfish<em> Mulloidichthys flavolineatus</em> (Lacepède, 1801) was studied to determine the spawning season and characteristics of its reproduction to support fisheries management. Adult fishes were caught with a gill net in the Egyptian Red Sea from January to December 2015. Specimens were dissected and sex ratio, gonado-somatic index, fecundity, maturity stages and egg diameter were determined. Larvae were collected with a plankton net at monthly intervals between January and December 2015. The gonado-somatic index was highest during summer (June-August) with that of females being significantly higher than that of males. Larvae were found between June and August with the highest densities in August. The spawning season of <em>Mulloidichthys flavolineatus</em> was recorded between June and August coinciding with increasing water temperatures. Spawning seasons of fish determined by fish larvae survey is in agreement with those determined by reproductive biology studies.
Goatfish,M. flavolineatus,Reproductive biology,Spawning season,Red Sea
https://ejabf.journals.ekb.eg/article_17159.html
https://ejabf.journals.ekb.eg/article_17159_039e4843f787574f2ec4895bbdaa5171.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
The Effect of dietary supplementation of Spirulina platensis and Chlorella vulgaris algae on the growth and disease resistance of the sea bass (Dicentrarchus labrax)
249
262
EN
Soha
S. Hasanein
Fish Physiology, Fisheries Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt
Norhan E. Saleh
Norhan E. Saleh
Fish Nutrition Lab., Aquaculture Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt
Heba
S. El-Sayed
Fish Repoduction and larval Rearing Lab.(Marine Hatchery), Aquaculture Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt
Amr
M. Helal
Phycology, Hydrobiology, National Institute of Oceanography and Fisheries, El-Qanater El-Khayria, Egypt.
10.21608/ejabf.2018.17160
The study was designed to investigate the protective effect of dietary <em>Spirulina platensis</em> and <em>Chlorella vulgaris</em> algal mixture (AM, 1:1), at three inclusion levels (3, 5 and 7%), in European sea bass diets as a supplement. 0.006 mg/l mixture of cadmium chloride and lead nitrate (TM, 1:1)was added to the rearing water as a pollutant.Results showed that the growth and feed utilization indices were not significantly different between fish fed diets containing algal mixture at inclusion levels in the presence of the pollutant and fish fed the control diet (Control<sub>l</sub>) meanwhile, fish fed control diet with heavy metals (Control<sub>2</sub>) addition showed the lowest growth and feed utilization indices. The body composition analysis showed no significant difference among groups. There was a significant decrease in the accumulation of Cd and Pb in white muscles. The lowest accumulations appear in fish fed diet<sub>3</sub>. A significant increase in the activity of antioxidant enzymes; SOD ,Catalase and Gpx were recorded in white muscles of diet<sub>1</sub>, diet <sub>2</sub> and diet <sub>3</sub> fish groups as compared to the control<sub>2</sub> group and the highest activity is in diet<sub>3</sub>.
Spirulina platensis,Chlorella vulgaris,Dicentrarchus labrax,growth rate,Protective Effect,disease resistance
https://ejabf.journals.ekb.eg/article_17160.html
https://ejabf.journals.ekb.eg/article_17160_1c57f284ddeaf0de7ed2a9235d0bda2f.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
09
01
Quality Evaluation of Three Types of Fish Pastirma during Frozen Storage
261
273
EN
Mohamed
Abou-Taleb
0000-0003-1344-5343
Fish Processing and Technology Lab., Fisheries Division, National Institute of Oceanography and Fisheries, Cairo, Egypt.
dr.m_abou_taleb@hotmail.com
Abdelrahman
S. Talab
Fish Processing and Technology Lab., Fisheries Division, National Institute of Oceanography and Fisheries, Cairo, Egypt.
Mohamed
A. Ibrahim
Fish Processing and Technology Lab., Fisheries Division, National Institute of Oceanography and Fisheries, Cairo, Egypt.
Fify
R. Anees
Fish Processing and Technology Lab., Fisheries Division, National Institute of Oceanography and Fisheries, Cairo, Egypt.
Maha
E. Genina
Fish Processing and Technology Lab., Fisheries Division, National Institute of Oceanography and Fisheries, Cairo, Egypt
Mostafa
M. Mahmoud
Fish Processing and Technology Lab., Fisheries Division, National Institute of Oceanography and Fisheries, Cairo, Egypt
Shimaa
M. Abou-Taleb
Fish Processing and Technology Lab., Fisheries Division, National Institute of Oceanography and Fisheries, Cairo, Egypt
10.21608/ejabf.2018.17162
The main objective of this investigation was to evaluate the quality of three types of fish pastirma prepared from common carp (<em>Cyprinus carpio </em>L.), kawakawa (<em>Euthynnus affinis</em>) and little tuna (<em>Euthynnus alletteratus</em>) during frozen storage at -18°C for three months. Physiochemical, microbiological and sensory properties of fish pastirma were determined. At zero time the results showed that, the values of pH, TVBN, TMA, TBA and TBC ranged between (4.82-5.42), (10.47-43.89 mg/100g), (2.56-10.70 mg/100g), (0.017-0.111 mg MDA/kg) and (1.23-1.77 logcfu/g); respectively. Also, results indicated that the values of pH, TVBN, TMA and TBA parameter were increased during storage period at -18°C and all samples did not exceed the maximum permissible levels as determined by the Egyptian Standard Specification for both freshwater and marine fish. <em>Staphylococcus aureus, E.coli,</em> totalcoliform were not detected during storage of three types of fish pastirma. In addition, total bacterial count was increased at the first month of storage then decreased till the end of storage but not exceed the MPL reported by ESS. Sensory evaluation did not change significantly (p>0.05) in both three types of fish pastirma. The study concluded that the effect of frozen storage at -18°C on fish pastirma quality properties varied between fish species and depended on the functional properties of fish muscles and all samples gain high organoleptic acceptance as an alternative and cheap product compared to the meat product.
Fish pastirma,Quality Criteria,Sensory evaluation,frozen storage,Quality evaluation
https://ejabf.journals.ekb.eg/article_17162.html
https://ejabf.journals.ekb.eg/article_17162_c520008be55648bbe27d8d8fa8518d0e.pdf
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
22
4
2018
11
01
Phytoplankton blooming in Lake Qarun in relation to chlorophyll a measured by fluorometric and spectrophotometric techniques
275
286
EN
Shymaa
Sabry
Zaher
National Institute of Oceanography and Fishieries
shymaasabry2@gmail.com
Ezzat
A. Ibrahim
National Institute of Oceanography and Fisheries, Cairo, Egypt.
10.21608/ejabf.2018.22002
Lake Qarun is a closed saline lake in the northern part of El- Fayoum Depression of Egypt. The objective of this work is to give a complete picture of lake biotic factors to alarm on dinoflagellates blooming phenomenon and to compare between chlorophyll <em>a</em> values measured by Fluorometer and Spectrophotometer. Monthly patterns in Chlorophyll <em>a</em> concentrations were measured during the period from May 2015 to April 2016, using two different methods; by fluorometric and spectrophotometric techniques. Phytoplankton species in Lake Qarun are heterogeneously distributed all over the year; dinoflagellates were the main dominant group, chlorophyll <em>a</em> frequently accumulated as distinct peak (336 μgl<sup>-1</sup>) in July 2015. The phenomenon of dinoflagellated blooming of <em>Prorocentrum micans, </em><em>Gymnodinium lantzschii </em>and <em>Exuviaell aapora </em>that turning the lake water to brown in colour, is recorded annually. The two methods used in detection of chlorophyll <em>a </em>during this work are accurate and simplified bring the picture in Lake Qarun. Actually, results of fluorometer are more sensitive and higher than those of spectrophotometer. In fact, high salinity, agricultural drainage water from drains and aquaculture activities turned the lake to variable, sensitive aquatic ecosystem, affecting significantly on phytoplankton structure and blooming dynamics determination. There is no record of toxicity effects or mass fish mortality in the lake due to this phenomenon.
Lake Qarun,Chlorophyll a,phytoplankton,Dinoflagellates,Blooming,Prorocentrum micans,Gymnodinium lantzschii
https://ejabf.journals.ekb.eg/article_22002.html
https://ejabf.journals.ekb.eg/article_22002_45fc4d5a740d2257ed6221e1e6d3ec88.pdf