ORIGINAL_ARTICLE
TRACE METALS BINDING TO HUMIC AND FULVIC ACIDS FROM SURFACIAL SEDIMENTS OF LAKE MANZALAH, EGYPT.
Twelve sediment samples were collected from Lake Manzalah during summer, 2004 to examine the influence of dissolved humic and fulvic acids on the distribution of trace metals in surficial sediments of the Lake. Trace metal concentrations in the humic acid extracted from the Lake sediments showed considerably high contents of iron (1046-3069 ng/g); copper (234-1498 jig/g) and zinc (110-394 jig/g) . The other trace metals (Co, Cd, Cr, Ni and Mn) revealed low concentrations, ranging from 11.3 to 156 ixgJg. The metal concentrations in fulvic acid were in the following order; Fe (465-2767 ^Lg/g); Cu (49.6-250.6 jag/g); Zn (49.3-186.6 ng/g); Cd (39.6-80.8 pg/g); Ni (20.4-153.4 jxg/g); Co (12.5-78.0 jxg/g); Mn (11.8-55.9 ^ig/g) and Cr (9.1-39.6 ng/g). The values of Cu and Cd percentage associated with the humic acid were high comparing with that reported in the other metals. This relative percentage was decreased for the same metals in association with fiilvic acid. In general, the results indicated that the amounts of trace metals in humic acids decreased as follows: Fe > Cu >Zn > Ni > Co > Cd > Mn > Cr. While in fulvic acid the order was: Fe > Cu >Zn > Cd > Ni > Co > Mn > Cr.
https://ejabf.journals.ekb.eg/article_1844_93c6ad6c50dd053be98a81c6e86d752e.pdf
2006-04-01
1
17
10.21608/ejabf.2006.1844
trace metals
Humic acid
Fulvic acid
Lake Manzalah
sediments
Mohamed
Okbah
1
National Institute of Oceanography and Fisheries, kayet bay, Alexandria, Egypt
AUTHOR
Mohamed
Hatned
2
National Institute of Oceanography and Fisheries, Suez, Egypt
AUTHOR
ORIGINAL_ARTICLE
ACCUMULATION OF A 70 KDA STRESS PROTEIN IN THE NILE TILAPIA, OREOCHROMIS NILOTICUS, AND ITS USE AS A BIOMARKER OF CU EXPOSURE
The induction of 70 kDa heat - shock protein (hsp70) in the Nile tilapia, Oreochromis niloticus, was investigated. The accumulation of hsp70 was determined in tissues of the liver, gills, spleen and heart of heat shocked fish. Other specimens were also exposed for 96 hr to four concentrations (1,0, 0.5, 0.1 and 0.001 mg/1) of Cu as copper sulphate and the accumulation of hsp70 was determined in the liver and gills. In both experiments, antibodies raised against hsp70 were employed as probes for dot-blot analysis and immunobinding assay of tilapia tissues. Decreasingly, hsp70 was abundant in the spleen, gills, heart and iiver of heat shocked fish. Also, data showed greater hsp70 level in the liver and gills of fish exposed to elevated Cu concentrations than the that of controls.These data suggest that hsp70 accumulation may hold a promise as a molecular indicator of contaminant exposure and may give an early warning of adverse biological effects. The present study indicates that the biomarker method is very easy to use, practical, rapid, and sensitive for assessing water quality in tropical water and is recommended for their incorporation into the future monitoring program.
https://ejabf.journals.ekb.eg/article_1846_b87c884ab874da467597f58003d752d7.pdf
2006-04-01
19
31
10.21608/ejabf.2006.1846
HSP70
fish
copper
Nile tilapia
biomarker
Khalid
Sharaf -El Deen
1
Zoology Department, Faculty of Science, Benha University, Egypt
AUTHOR
ORIGINAL_ARTICLE
FISHERIES MANAGEMENT OF THE THINLIP GREY MULLET LIZA RAM AD A AND GOLDEN GREY MULLET LIZA AURATA FROM LAKE BARDAWIL, EGYPT
Monthly samples of Liza ramada and L. aurata were collected from Lake Bardawil during the period from April to December 2004. to study their population dynamics and to assess the fishery status of their stocks. The total length of L. ramada and L. aurata ranged from 16.9 to 42 cm and 16 to 30.5 cm respectively. Otoliths were used to estimate their ages. The age distribution covered age groups I to V for L. ramada and I to III for L. aurata with age groups II and I being the most frequent for L. ramada and L. aurata, respectively. The von Bertalanffy growth equations were Lt= 44.14 (3-e*d5Ul*n-9) for L ramada and L,= 33.77 (I-e °* ltl"r-J*j4J) for L. aurata. The respective instantaneous annual rates of total. natural and fishing mortality were 1.22. 0.16 and 1.06 year"1 for L. ramada and 1.36, 0.22 and 1.14 year"1 for L. aurata. Exploitation rate was estimated as 0.87 and 0.84 year"1 for L ramada and L. aurata respectively. The estimated lengths at first capture were 18.45 and 16.92 cm. while the length at first sexual maturity was 28.71 and 23.11 cm for L. ramada and L. aurata respectively. The relative yield per recruit and relative biomass per recruit analysis indicated that the stocks of L. ramada and L. aurata at Lake Bardawil were over-exploited and their respective current exploitation rate should be reduced by about 54% and 52.4% and their respective length at first capture should be raised to 30 and 25 cm
https://ejabf.journals.ekb.eg/article_1847_43e3759dd64c5c245cd036b719774c4e.pdf
2006-04-01
33
53
10.21608/ejabf.2006.1847
Lake Bardawil
Mugilidae. Liza ramada
Liza aurata
Population dynamics
Stock assessment
Management
Sahar
Mehanna
1
National Institute of Oceanography and Fisheries, Suez.
AUTHOR
ORIGINAL_ARTICLE
THE MOLECULAR CHANGES OF HEPATOCYTES IN TILAPIA ZILLH UNDER THE EFFECT OF THE AGRICULTURAL AND INDUSTRIAL POLLUTION IN THE RIVER NILE, EGYPT
T iver specimens were taken from seventy fish of, Tilapia zillii9 living -■—'in three different localities in Qalyobya govemorate; unpolluted, agricultural-polluted and industrial-polluted waters of the River Nile. Water analysis was carried out to evaluate the heavy metals in these tested localities. Polymorphism of liver proteins was revealed by SDS-PAGE electrophoresis. Also, DNA damage was detected by counting the fragmentation percent. Results showed that the measured heavy metals were higher in the agricultural and industrial localities than in control; and sometimes exceeded the permissible concentrations. Fractions of liver proteins showed disappearance and polymorphism in the fish especially that was very close to the outlets of agricultural and industrial drainage water. Additionally, both agricultural and industrial pollution caused an increase in the percentage of DNA fragmentation, however the impact of industrial pollution was higher than that of agricultural pollution,The study suggested that there is clear molecular changes in T. zillii; this declares the use of these molecular parameters in diagnosing pollution.
https://ejabf.journals.ekb.eg/article_1848_49c11598d204b568917a976655b34b58.pdf
2006-04-01
55
67
10.21608/ejabf.2006.1848
Tilapia zilliu pollution
Electrophoresis
Liver
Khalid
Sharaf-Eldeen
1
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt
AUTHOR
Magda
El-Ezabi
2
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt
AUTHOR
Amani
Al-Bohaissi
3
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt
AUTHOR
ORIGINAL_ARTICLE
POPULATION DYNAMICS OF LUTJANUS LINEOLATUS (FAMILY: LUTJANIDAE) FROM THE BITTER LAKES, SUEZ CANAL, EGYPT
Atotal of 837 Lutjanus limolatits was collected monthly from Bitter Lakes during October 2002 through October 2003 to study age and growth, mortality, relative yield per recruit and relative biomass per recruit Age was determined from otolith's reading and the results showed that the maximum life span of L. iineolaius is 3 years. The growth parameters were estimated as K = 0.60 year-1, Loo = 22.92 cm TL and to = -0.27 yr. The annual rates of total, natural, fishing mortality and exploitation rate were calculated as 2,5, 0.6, 1.9 and 0.76 year-1 respectively. The high level of both fishing mortality and exploitation rate indicated the high level of exploitation of this species. The length at first capture Lc was 31.1 cm TL.The relative yield per recruit analysis showed that the stock of L. lineolatus is over-fished. To maintain this valuable fish resource, the present fishing effort
https://ejabf.journals.ekb.eg/article_1850_ab8f6471d066ec36c3f0c574a5e4ebad.pdf
2006-04-01
79
92
10.21608/ejabf.2006.1850
Suez Canal
Bitter Lakes
Lutjanus Imeoiatus^ age and growth
mortality and exploitation rates
relative yield per recruit
relative biomass per recruit
Amal
Amin
1
National Institute of Oceanography and Fisheries, Suez, Egypt
AUTHOR
ORIGINAL_ARTICLE
A STUDY ON MOLECULAR BIOLOGY OF TILAPIA SPECIES AND THEIR HYBRIDS IN THE RIVER NILE, EGYPT.
Morphometric, meristic and DNA ripoprinting analyses of Tilapia species and their hybrids inhabiting the River Nile were examined. The obtained morphometric data evoke striking similarities and overlapping among tilapia species. Accordingly it could not able to differentiate tilapia species. The obtained data of meristic characters reveal that tilapia species could be differentiated into four species {Oreochromis niloticus, O. auraeus, Sarotherodon galilaeus and Tilapia zillii). The lateral line scales differed significantly among the four-studied tilapia species. While the number of fin rays in the- dorsal and anal fins differentiate significantly three Tilapia species (Confusion between 0. niloticus and 0. auraeus). Furthermore, the study used the technique of restriction fragment length polymorphism (RFLP) of nuclear small sub-unit ribosomal RNA (18SsrRNA ) gene. The PCR-RFLP data provide a unique pattern for each examined species with a specific restriction enzyme. So, it could be possible to detect two hybrids of tilapia fish which are given HI and H2 symbols. The endonucleases Sacll and Apal differentiate HI and H2, respectively. Furthermore, the analyses of PCR-RFLP data indicate that the HI are closer to O. niloticus and S. galilaeus, whereas the H2 is phylogenetically closer to O. auraeus and T. zillii. The data of this research evoke a monophyiogenetic relationship of all the studied Tilapia species.
https://ejabf.journals.ekb.eg/article_1852_1e0a8862b766f2b6e99ced379c32137b.pdf
2006-04-01
93
116
10.21608/ejabf.2006.1852
Tialpiay 18srDNA
RFLP
morphometric data
meristic
Hybrids
Sabry
El-Serafy
1
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt.
AUTHOR
Nassr-AIIah
Abdel-Hamide
2
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt.
AUTHOR
Mona
Azab
3
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt.
AUTHOR
Mohammed
Awwad
4
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt.
AUTHOR
ORIGINAL_ARTICLE
IMPACT OF WATER QUALITY DETERIORATION ON CORAL REEF COMMUNITY STRUCTURE IN THE NORTHERN RED SEA, EGYPT
Fifteen water quality parameters and nine coral community variables were assessed in eight reef sites along the Red Sea coast of Egypt. Coral reef environments are suffering of stress from increased anthropogenic activities, particularly in the vicinity of heavily populated and touristic areas such as Hurghada, Ain Al-Sukhna and Sharm El-Sheikh. Increased terrestrial runoff of sediments, nutrients, organic matter and other pollutants are the main causes of water quality deterioration in the investigated reef sites. The results showed that bioerosion of corals by sea urchins, and enhanced abundance of macroalgae and consequent competitive overgrowth of corals were the major reasons of coral damage observed in the area of study. Population density of sea urchins exhibited significant and positive correlations with the majority of eutrophication parameters. Salinity was significantly and negatively correlated with live hard coral cover. Exceeding levels of DIN and TSM above the threshold concentrations for eutrophication were reported to be indirectly and adversely affecting coral reefs through stimulating the growth of macroalgae, enhancing sea urchin density and reducing water transparency. The results support the predictions of bottom-up hypothesis that confirm the critical role of eutrophication in structure of coral reef community.
https://ejabf.journals.ekb.eg/article_1854_33da5bc63b696a90a0222d51e8217f43.pdf
2006-04-01
117
146
10.21608/ejabf.2006.1854
water quality
corals
Eutrophication
sea urchins
Macroalgae
Nutrients
Abdel-Hamid
Ali
1
National Institute of Oceanography and Fisheries, Suez, Egypt
AUTHOR
Mohamed
Hamed
2
National Institute of Oceanography and Fisheries, Suez, Egypt
AUTHOR
ORIGINAL_ARTICLE
COMPARATIVE STUDY ON ELECTROPHORETIC PROTEIN PATTERN CHARACTERIZATION OF TILAPIA SPECIES IN THE RIVER NILE, EGYPT.
This study deals with the electrophoretic characterization of Tilapia species using plasma and muscle proteins, as a taxonomically tool to differentiate Tilapia species that live in Egyptian habitat of the River Nile. These species are Oreochromis nilotrcits ( O. nihticus ) , O. aureus fiarotherodon galilaeits ( S. galilaeus ) and Tilapia zillii ( T. zillii). The data of electrophoretic protein separation indicated that each species has a characteristic specific pattern with more common bands for all species as well as species specific bands characteristic for each one. In this study, the protein of plasma and muscle were examined either treated with sample buffer or not treated. The results indicated that the protein pattern among the closer species exhibits common characters. In this regard, the obtained protein pattern of O. niloticus looks like that of O. aureus indicating a monophylogenetic origin of these species. Whereas, less degree of similarity was recorded between T. zillii and other species, indicating a genetic distance between this species and the rest of Tilapia species.
https://ejabf.journals.ekb.eg/article_1856_01caa2b9dc2262b6301a33c8dcf89162.pdf
2006-04-01
147
178
10.21608/ejabf.2006.1856
Tilapia species
protein electrophoresis
plasma
muscle
monophylogenetic. River Nile
Sabry
El-Serafy
1
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt.
AUTHOR
Nassr-AHah
Abdel- Hamide
2
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt.
AUTHOR
Mona
Azab
3
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt.
AUTHOR
Mohammed
Awwad
4
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt.
AUTHOR
ORIGINAL_ARTICLE
COMPARATIVE STUDY ON THE LEVELS OF HEAVY METALS IN THE BITTER LAKES AND LAKE TIMSAH, (SUEZ CANAL) IN RELATION TO THE REPRODUCTIVE CYCLE OF THE RABBIT FISH SIGANUS RIVULA TUS.
The concentrations of Cu, Zn, Pb, Cd, Fe, Mn, Cr, Ni and Co in muscles, liver and gonads of the marine rabbit fish Siganus rivulaivs from the Bitter lakes and lake Timsah, (Suez Canal) were investigated seasonally, over the course of one year (November 2002 - August 2003). Muscles recorded the lowest concentrations of metals than other organs which ranged between 0.22-0.76 and 0.27 - 1.23, 1.95 - 3.67 and 3.01 -5.08, 0.09 -0.43 and 0.24 -0.51, 0.04 -0.16 and 0.11 -0.37, 4.32 -5.66 and 5.01 - 10.33, 0.12 - 0.29 and 0.26 - 1.35,0.06 - 0.09 and 0.05 - 0.16, 0.02 - 0.39 and 0.03 - 0.51 and 0.05 - 0.24 and 0.06 - 0.31 jig/g wet weight for Cu, Zn, Pb, Cd, Fe, Mn, Cr, Ni and Co in Bitter lakes and lake Timsah, respectively. The highest concentrations of all studied metals were found in gonads. Variation of metals in fish muscles through different seasons indicated that the most studied metals exhibited their highest value during the hot seasons (spring & summer). Gonadosomatic index (GSI) and its relationship with metal accumulation was calculated for the studied fish. By using seasonal changes in the values of gonadosomatic index, it was found that their spawning season is during spring.
https://ejabf.journals.ekb.eg/article_1857_7cb74f7ad738ff912454b39468e2f2cc.pdf
2006-04-01
179
193
10.21608/ejabf.2006.1857
Heavy metals
Sigcmus rivulatus
reproductive cycle
Bitter Lakes
Lake Timsah
Suez Canal
Magdy
EL-Halfawy
1
National Institute of Oceanography and Fisheries, Suez, Egypt
AUTHOR
Waheed
Mahmoud
2
National Institute of Oceanography and Fisheries, Suez, Egypt
AUTHOR
Ami
Ramadan
3
National Institute of Oceanography and Fisheries, Suez, Egypt
AUTHOR
Yousry
Soliman
4
National Institute of Oceanography and Fisheries, Suez, Egypt
AUTHOR
ORIGINAL_ARTICLE
صور المعادن الثقيله برواسب القاع في بحيره المنزله-مصر
https://ejabf.journals.ekb.eg/article_1858_ccfffada871ce9b26e77a9eb9bc5d8de.pdf
2006-04-01
1
9
10.21608/ejabf.2006.1858