ORIGINAL_ARTICLE
COMPARATIVE STUDY ON SOME HISTOLOGICAL AND BIOCHEMICAL ASPECTS IN FISHES FROM QARUN AND BARDAWIL LAKES
M^le specimens of Tilapia zillii. Magil cephalus. Liza ramada and Soiea egyptiaca were collected from Ayoub and Shakshouk Stations at the west and middle of Qarun Lake. At the same period, other male specimens of M. cephalus. L. ramada and Solea vulgaris were collected from Bardawil Lake. The histological and biochemical findings can be summarized as follows:Histological examination of gills, liver and kidney of the four studied fish species in both lakes showed variable pathological changes. Bardawil Lake fish showed more damage. The histopatho logical features are discussed,Biochemical significant differences were found between the fishes collected from the two stations of Qarun Lake. T. zillii showed significant higher values of liver total proteins (TP) and inorganic phosphorus (P) than the other three species, while L. ramada showed significant higher values of liver alkaline phosphatase (ALP) and calcium (Ca). Muscle total cholesterol (TCh), low-density lipoprotein (LDL) and Ca were higher in M cepkalus but TP, high-density lipoprotein (HDL) and P in L. ramada; ALP and total lipids (TL) in Solea egyptiaca and triglycerides (TG) in Z zillii,Mugil cephalus of Bardawil Lake showed significant higher values of muscle TP, ALP, TL, TG, HDL, LDL and Ca than the other two fish species- Muscle TCh and P were significantly higher in 5. vulgaris and L. ramada respectively. Also S. vulgaris showed significant higher values of liver TP and P and L. ramada showed significant higher values of liver ALP and Ca.
https://ejabf.journals.ekb.eg/article_1807_03421cfdbd29384d46e0f4a5bb72b1a6.pdf
2004-09-01
1
35
10.21608/ejabf.2004.1807
Qarun Lake. Bardawil Lake. Tilapia ziliit\ Mugii cephalus
Liza ramada
Solea sp
Histology. Biochemistry
liver and muscle
Hunan
Gaber
1
National Institute of Oceanography and Fisheries. Fish Res, Station. Cairo.
AUTHOR
Esam
Rizkalla
2
Biochemistry, Nutritional deficiency Diseases and Toxicology Dept.. Animal Health Res. Insi.. DokkL Giza.
AUTHOR
Basem
Fahmy
3
Biochemistry, Nutritional deficiency Diseases and Toxicology Dept.. Animal Health Res. Insi.. DokkL Giza
AUTHOR
ORIGINAL_ARTICLE
IMPACT OF ENVIRONMENTAL PARAMETERS ON BENTHIC INVERTEBRATES AND ZOOPLANKTON BIODIVERSITY OF THE EASTERN REGION OF DELTA COAST AT DAMIETTA, EGYPT
Environmental parameters as well as heavy metals (cadmium, lead. copper and zinc) in water and sediment have been studied in the eastern section of Delta coast-Mediterranean Sea, This research has been done during four seasons from summer 2002 to spring 2003. Both zooplankton and benthic invertebrates abundance in sediment have been estimated in Damietta coast. Most of environmental parameters showed higher concentrations during summer and autumn 2002 than that values recorded in winter and spring 2003. Dissolved oxygen concentration was decreased to reach (0 mg/1) at New Damietta drain, while BOD concentration increased at the "same site. due to the increased quantity of industrial drainage water which adversally affected the biodiversity. Lead values in water showed high concentration (0.08 mg/1) at sites 4 & 2 during 2002 & 2003 respectively. This increase may be due to the activity of maritime ports for both commercial transport and fishing at Ezbet el-Borg. Heavy metals were magnified 100-1000 times in sediment more than that recorded in water. The concentrations of heavy metals in water and sediment were in the order of Zn> Cu> Pb>Cd. Sites 4 & 3 showed higher abundance of invertebrate species than other sites. Biodiversity of invertebrates in sediment was higher at sites 1 and 3. and that may be attributed to the high concentration of DO in water and TOC in sediment. Twenty two species of zooplankton of 6 phyla were identified. The biodiversity of zooplankton during spring 2003 was hieher than that recorded durinu summer 2002.
https://ejabf.journals.ekb.eg/article_1808_aa30836641a974b3b51476d0234c2318.pdf
2004-09-01
37
52
10.21608/ejabf.2004.1808
Environmental parameters
Heavy metals
biodiversity
Sediment
aquatic invertebrates
zooplankton. Mediterranean Sea
Mohamed
Zyadah
1
Environmental Sciences Depart., Damietta Faculty of Science, Mansoura University. 34517, New Damietta^Egypt
AUTHOR
Mahmoud
Ibrahim
2
Environmental Sciences Depart., Damietta Faculty of Science, Mansoura University. 34517, New Damietta^Egypt
AUTHOR
Amany
Madkour
3
Environmental Sciences Depart., Damietta Faculty of Science, Mansoura University
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF CLOSTRIDIUM PERFRINGENS INFECTION ON SOME BIOCHEMICAL, PHYSIOLOGICAL AND PATHOLOGICAL PARAMETERS IN OREOCHROMIS NILOTICUS
Nile tilapia (Oreochromis niloticus) was injected intraperitoneally with types A, D and A+D of Clostridium perfringcns under .controlled laboratory conditions. The biochemical and haematologicai analyses were carried out on the 3rd, 7lJl and I4lh days post injection. The obtained results showed that:1. Clinically, the infected fish showed loss of appetite, sluggish and erratic swimming, loss of equilibrium and listless. At the site of injection, there was brownish caseous material which easily separated from the muscle. Gills were congested. Internally, the organs were friable and had a generalized hyperemic appearance.2. Histopathologically. the striated muscle, at the site of injection showed edema and necrosis with leucocytic infiltration. The gills showed sloughing of secondary lamellae and desquamation of the epithelial covering of secondary lamellae.3. Significantly decreased numbers of erythrocytes were detected after injection with C perfringens type A or D at all periods. Sporadically significant decreases in haemoglobin and haematocrit were found. Other haematologicai indices such as mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) revealed inconsistent fluctuations.4. The leucocytes were decreased significantly in fish infected with C perfringens types D and A+D on the 7th and 14lh days respectively.5. Plasma and muscle total proteins showed a significant decrease whereas liver total proteins recorded a significant increase in comparison with the corresponding control values.6. Plasma glucose was significantly increased in fish injected with C perfringens type D at all periods, but significantly decreased with the combination of A+D.7. Plasma total lipids were significantly decreased in fishes injected with C perfringens type D as well as with the combination of A+D.8. Plasma alkaline phosphatase activity significantly decreased in all groups at al] periods studied.9. A genera] tendency of a significant decrease was observed in plasma aspartate aminotransferase (AST) activity paralleled with an increase in alanine aminotransferase (ALT) activity after injection with the two strains of C perfringens or their combination. There were some changes following this trend with respect to liver AST and muscle ALT.In* conclusion. Clostridium perfringens bacteria caused a high mortality rate amone. the cultured fish, while the drastic effect of the mild bacteria infection was inflected more on the fish physiological status and as a consequence decreases the farm production. Either mild or severe bacteria! infection should receive an immediate eye of inspection.
https://ejabf.journals.ekb.eg/article_1809_36af92d27e0d885bec72dd5f31d3cdfd.pdf
2004-09-01
53
84
10.21608/ejabf.2004.1809
Oreochromis niloticus
Clostridium parfringans.haematology/histopathology. enzyme activities
Esam
Rizkalla
1
Food Deficiency Diseases. Biochemistry & Toxicology Dept.. Animal Health Res. Institute. Agric. Res. Cen., DokkL Giza.
AUTHOR
Addel
Shalaby
2
Fish Physiology Dept., Central Laboratory for Aquaculture Res.. , Agric. Res. Cen.,
AUTHOR
Ahmad
Ashram
3
Fish Diseases Dept.. Central Laboratory for Aquaculture Res., Agric. Res. Cen
AUTHOR
Afaf
Yanni
4
Bacteriology Dept.. Animal Health Res. Institute, Agric. Res. Cen.
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF FATTY ACIDS ON THE RESPONSE OF THE LIVER MICROSOMAL BIOTRANSFORMING ENZYMES IN THE NILE TILAPIA OREOCHROMIS NILOTICUS EXPOSED TO QUINTOZENE
This study was conducted to investigate the impact of nutritional environmental factors on the efficacy of microsomal biotransforming enzymes to organic xenophobic in the freshwater fish O. nilolicu exposed to the pesticide quintozene Two groups of fish, one was maintained on a normal diet (A) and the other on a diet supplemented with fish liver oil (B) for 4 weeks. Then the fishes were treated with quintozene by interperitonial injection of $50 |il (30 mg/kg of body weight) and the responses of liver microsomal biotransforming enzymes were assessed after 48 hours.Treatment with quintozene of fishes maintained on diet B. produced an increase in microsomal contents of cytochrome P-450 and cytochrome b$ reaching 1.44 and 2.88 fold respectively compared with group A, whereas, activities of 7-ethoxyresorufin -O- deethylase (EROD) and 7- ethoxycouma'rin- deethiase (ECOD) reached 2.8 to 5.11 fold respectively.Similarly, the phase II enzymes, Uridine diphosphoglucuronyl - transferase (UDPGT) activity was less induced in fishes fed diet A than diet B; whereas an increase of 1.64 and 4.7 fold respectively were noticed. Cytosolic glutathione- S- transferase (GST) was not affected by either treatments.In addition, a fish liver oil rich dietary supply of polyunsaturated fatty acids (PUFAs) may prevent these changes, even in a long- term subchronic exposure.The results indicated that fatty acid composition of diet of Oreochromis nilodcus can affect significantly the biotransforming enzymes in response to xenobiotic pollutants.
https://ejabf.journals.ekb.eg/article_1810_83f7af01032bc8a809654dadce4e2f78.pdf
2004-09-01
85
105
10.21608/ejabf.2004.1810
Cytochrome P-450
Cytochrome b5
EROD. ECOD
UDPGT . GST. Polyunsaturated fatty acids (PUDFAS). microsomes
cytosoL Oreochromis nilodcus
Shchata
Elowa
1
Department of Zoology. Faculty of Science, Helwan University.Egypt
AUTHOR
ORIGINAL_ARTICLE
ECOLOGY OF EIGHT SPECIES OF FRESHWATER OSTRACODS (CRUSTACEA) FROM QENA GOVERNORATE, UPPER EGYPT
The present work deals with the ecology of eight species of freshwater ostracods (Cypridopsis vidiui, Potamocypris variegattf. Hemicypris dematomarginata, llyocypris gibha, Uyocypris hiplicata, Fabaeformisccmdona holzkampfu Pseudocandona semicognita and Limnocythere inopinata) collected from Qena Govemorate. Monthly samples were collected for a period of one year (from July, 2000 till June, 2001) from four sites. Some ecological factors were measured during the period of investigation and correlated with the density of the ostracod species studied. Statistical analysis was performed by using SPSS software package (version 9) and the model equations controlling the density and the physical factors measured for the studied species were deduced.
https://ejabf.journals.ekb.eg/article_1811_33e6a25ce3c185ec9347c2c314e4e00f.pdf
2004-09-01
107
122
10.21608/ejabf.2004.1811
Ecology
freshwater ostracods. Qena. Upper Egypt
Mohammad
Hussein
1
Faculty of Science, Assiut University, Assiut. Egypt
AUTHOR
Ahmad
Obuid-AIlah
2
Faculty of Science, Assiut University, Assiut. Egypt
AUTHOR
Amal
Mahmoud
3
Faculty of Science. South Vaiiey University, Qena. Upper Egypt
AUTHOR
Heba
Fangary
4
Faculty of Science. South Vaiiey University, Qena. Upper Egypt
AUTHOR
ORIGINAL_ARTICLE
REPRODUCTIVE CYCLE AND MARICULTURE POTENTIAL OF THE RABBITFISH SIGANUS CANALICULATUS IN SAUDI ARABIA
The present work was meant to the reproductive cycle of the rabbitfish Siganus canaliculatus, one of the economically important marine fish inhabiting the Arabian Gulf of Saudi Arabia coast. The future expectations of development of Siganus mariculture were described. This study revealed the sex ratio, gonads maturity stages, size at first sexual maturity, spawning season, ova-diameter and fecundity. The length at first sexual maturity (Lm50) was found to be 18 cm and 19 cm for males and females, respectively. Fishes larger than 22 cm total length of both sexes were found to be fully mature. Although, the gonado-somatic index (GSl) of Siganus canal iculatus differed significantly (P > 0.05) between males and females, it exhibited the same trend for both, where GSI reached its maximum values (peaks) of 18.0 and 22.5 during May for males and females, respectively. The spawning season was found to be short and occurred during the period from April to June, with a high peak during May, which means that one spawning season was exhibited. The egg-diameter distribution was significantly variable in the ripe ovaries with largest egg diameters ranging from 0.3 to 0.4 mm, which constitute about 88 % of the total eggs and revealing one peak during May. Absolute fecundity was increased with increasing length, weight and age of the fish. Fecundity exhibited a curvilinear trend or exponential equation with fish length, fish weight and fish age. The mean sex ratio (Males: Females) for the whole year was 1:1.13. It was recommended that capturing of fish smaller than 20 cm total length should be prohibited to protect the spawning stock biomass of rabbitfish S. canaliculatus on the long run. Also, many aspects for mariculture potential were included.
https://ejabf.journals.ekb.eg/article_1812_43901a73a0a719f8d4efa5a602344b55.pdf
2004-09-01
123
143
10.21608/ejabf.2004.1812
Rabbitfish. Sigamis canaticuictus* reproduction
fisheries. Arabian Gulf
Adel
Tharwat
1
Department of Animal Production, Faculty of Agriculture, Cairo University. Giza. Egypt
AUTHOR
ORIGINAL_ARTICLE
دراسه مقارنه لبعض المؤشرات الهستولوجيه والکيميائيه الحيويه لأسماک بحيرتي قارون والبردويل
https://ejabf.journals.ekb.eg/article_1813_1c4d88234730d8bdea5bfe38d03528e8.pdf
2004-09-01
1
6
10.21608/ejabf.2004.1813