eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
1
15
10.21608/ejabf.2020.87108
87108
Original Article
Abundance and diversity of amphipod species associated with macro-algae at Ras-Mohamed, Aqaba Gulf, Red Sea, Egypt
Mostafa Kh. Gabr
1
Amr F. Ziena
2
Ahmed M. Hellal
3
Amphipod community is considered one of the main and important invertebrate communities associated with macro-algae in intertidal zone. So, the present work aimed to study the seasonal abundance and diversity of amphipod species inhabited macro-algal hosts in Ras Mohamed protectorate. Samples and data of the present study were collected seasonally from three sites inside this protectorate.
Results showed that twenty-three amphipod species associated with 15 macro-algal species (red, brown, and green algae) were recorded. The algal species fluctuated during different seasons. The highest abundance and diversity of amphipod species were recorded with species that associated with the red algae. Some amphipod species were found to have a high selection for their hosts. Also, the present study showed that some physical factors (such as type and shape of the place of living habitat, size of host plants patches, seasonality} and chemical factors (secondary metabolic chemicals) playing the main role in the evolution of the amphipod community by effecting on the abundance and diversity of amphipod species.
https://ejabf.journals.ekb.eg/article_87108_31a640762288664ee086b7c695fb0f34.pdf
Amphipod species
Macro-algae
Ras-Mohamed
Aqaba Gulf
Red Sea
diversity
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
17
28
10.21608/ejabf.2020.87813
87813
Original Article
Determination of Endogenous Formaldehyde in Moonfish (Lampris guttatus) During Frozen Storage
Giri R. Barokah
1
Farida Ariyani
idapoernomo@yahoo.co.id
2
Singgih Wibowo
paksingh@yahoo.com
3
Hedi I. Januar
4
Umi Annisah
umi.anissah@gmail.com
5
Formaldehyde is a carcinogenic compound that harmful to health and often misused as a food preservative in Indonesia. However, in most of the foods including fish, formaldehyde can be formed endogenously or naturally during storage. Recently, Moonfish (Lampris guttatus) from Indonesia has been exported. Meanwhile, during delayed transportation to importing countries, moonfish need to be kept frozen up to several months This condition leads to the formation of formaldehyde. The aim of this research is to study the pattern of endogenous formaldehyde formation in moonfish during the frozen storage period. The study was conducted by observing the formation of endogenous formaldehyde as well as the formation of DMA (dimethylamine), TVB (total volatile base), TMA (trimethylamine), and TMAO as parameters related to the formation of endogenous formaldehyde in moonfish. The parameters were observed periodically shortly after the fish died until six months in frozen storage. The results showed that the content of formaldehyde, TVB, TMA, and DMA in moonfish during six months of frozen storage increased every month. The formation of natural formaldehyde in the moonfish was inline with quality degradation. Moonfish is still suitable for consumption based on its freshness level up to one month in frozen storage, but based on the formation of formaldehyde, the level of consumption should consider the calculation of maximum daily intake based on specified reference dose.
https://ejabf.journals.ekb.eg/article_87813_60f5fac13839e603901755a799d30558.pdf
Formaldehyde
Lampris guttatus
Deterioration process
frozen storage
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
29
43
10.21608/ejabf.2020.88238
88238
Original Article
Antibacterial Activity, Biochemical Effect and Tissue Residue of Fourth Generation Cephalosporin Used in Treatment of Nile Tilapia Fish Against Bacterial Infection
Salah M. Aly
1
Waleed F. Khalil
2
Samar M. Ghaleb
3
Antimicrobial drugs are used to control bacterial infections among fish in aquaculture and misuse could be associated with the spread of antimicrobial resistance and tissue residues with a negative impact on humans and animals including fish. This study was conducted to evaluate the effect of cefquinome on Aeromonas hydrophila and some biochemical parameters in tilapia fish, together with estimating its residue in serum, muscles, and liver using high-performance chromatography. One hundred and thirty-five Nile tilapia fish (Oreochromis niloticus) were used and divided into 3 equal groups. Fish of group 1 was a negative control, group 2 was a positive (infected with Aeromonas hydrophila) and group 3 was infected with the same bacteria and treated with Cefquinome sulphate (Cobactan® 2.5%) as a single intramuscular dose of 10 mg/kg body weight. Cefquinome showed an in-vitro inhibition zone of 29.3± 0.26 mm against A. hydrophilla, and the minimum inhibitory concentration was 2 µg/ ml. While, in-vivo; the mortality rate among infected fish was decreased after treatment by cefquinome from 66.6 % to 17.7 %, a slight recovery has been recorded in biochemical parameters at 7th to 14th day post-medication. Serum and tissue residue of cefquinome reached under the limit of detection at 120 hrs. post-medication while still detected in the liver even after 120 hrs post medication. It could be concluded that cefquinome had a good effect against Aeromonas hydrophila infection in tilapia. Fish flesh could be suitable for human consumption 5 days of post-cefquinome medication.
https://ejabf.journals.ekb.eg/article_88238_b3828d1891170b27fa405d3dd26778b0.pdf
cefquinome
Tilapia fish
Aeromonas hydrophila
resistance
Antibacteria
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
45
52
10.21608/ejabf.2020.87819
87819
Original Article
Burrow structure and utilization of Periophthalmodon schlosseri (Pallas, 1770) from Tran De coastal area, Soc Trang, Vietnam
Lam T. Tran
1
Y T. N. Nguyen
2
Tien T. K. Nguyen
3
Quang M. Dinh
4
This study was conducted from October 2017 to March 2018 in Mo O territorial waters, Tran De District, Soc Trang province to contribute data of burrow morphology and utilization of the species Periophthalmodon schlosseri that was one of the important economical fish species in the Mekong Delta. The burrow morphology and dimensions were obtained by analyzing 30 casting burrows molded by polyester resin. The burrow utilization was determined by analyzing casting burrows and observing activities of fish in its habitats. The analysis result showed that burrows of this fish were various in shapes such as U, I, and J. The burrow had 1-2 opening with a large mound made from mud pellets, and 0-1 bulbous chamber. Dimensions of the chamber did not display seasonal and spatial changes, however, they varied with burrow shape. Furthermore, the burrow dimensional variations did not change with the interaction of season, studied sites, and shape variables. This fish used burrows as a shelter for spawning and avoiding predators. The results can be used for further research on the artificial reproduction of this species.
https://ejabf.journals.ekb.eg/article_87819_7d5c04fece2af2fe8bd745bf954630e9.pdf
Burrow
Mekong Delta
mudskipper
Soc Trang
tide
Usage
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
53
59
10.21608/ejabf.2020.91657
91657
Original Article
Length-weight relationship and relative condition factor of the Sultan fish, Leptobarbus hoevenii broodstock farmed in earthen ponds
Sajeenuth Srithongthum
sajeenuth91@gmail.com
1
Thumronk Amornsakun
thumronk.a@psu.ac.th
2
Poramat Musikarun
mporamat@gmail.com
3
Pornpanom Promkaew
pornpanomp@hotmail.com
4
Hsein-Loong Au
davidau940903@gmail.com
5
Gunzo Kawamura
prof.gunzo@gmail.com
6
Mohammad T. M. Lal
7
Leong-Seng Lim
leongsen@ums.edu.my
8
The present study was aimed to assess the general health condition of Sultan fish, Leptobarbus hoevenii broodstock through their length-weight relationship (LWR) and relative condition factor (RCF). This broodstock was poly-cultured with the other carp’s species in earthen ponds. Totally, 95 broodstock (35 females and 60 males) were examined in this study. The total length (cm) and body weight (g) ranges of the L. hoevenii broodstock were 32.8 – 43.0 cm and 460 – 1,400 g for the females, while 30.0 – 44.5 cm and 285 – 1,300 g for the males, respectively. The b value of the females was 3.10 (> 3.0), indicating that they have attained positive allometric growth. In opposite, the males and the overall populations have attained negative allometric growth as their b value was 2.66 and 2.92 (< 3.0), respectively. All of these LWRs were significant at α = 0.01 with their correlation coefficient (r) values at 0.88 - 0.90. However, the 95% confidence interval of these b values were in the range of 2.32 – 3.64; they included 3.0 and overlapped each other. This result showed that these b values were not significantly different, indicating that the fish has achieved ideal growth. In addition, the relative condition factors of both females and males have achieved more than 1 (1.04 and 1.01, respectively), indicating that they have achieved their expected weight. Further observation on the LWR of the male L. hoevenii broodstock from the wild is necessary to confirm its optimum b value. Overall, the b value of the broodstock was 2.92 (close to 3), and their RCF value was 0.98 (close to 1). Therefore, the broodstock can be considered in good health condition. However, the influence of poly-culture management on the LWR of this broodstock should be examined in future studies.
https://ejabf.journals.ekb.eg/article_91657_fd29197525827e9e5afcdfaaeb1f5a22.pdf
Health condition
mad barb
Broodstock
Leptobarbus hoevenii Sultan fish
Freshwater aquaculture
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
61
73
10.21608/ejabf.2020.87814
87814
Original Article
Distribution of potentially pathogenic Acanthamoeba isolates in the environment of Helwan University, Egypt
Heba Koteit
hebakoteit@yahoo.com
1
Shehata E. Elowa
2
Ahmad Z. Al-Herrawy
3
Acanthamoeba species are free-living amoebae having worldwide distribution. These amoebae can cause granulomatous amoebic encephalitis and amoebic keratitis in humans. They can produce proteases that are considered virulence factors. Acanthamoeba can also harbor pathogenic bacteria, fungi, and viruses.
The objective of this study is to evaluate the presence of Acanthamoeba in the environment of Helwan University, Egypt.
Six types of samples (tap water, irrigation water, wastewater, swabs from surfaces, soil, and air) were collected, processed, and cultured on non-nutrient agar medium. Positive plates for Acanthamoeba were subcultured, purified and amoebae were identified morphologically and confirmed by PCR using Acanthamoeba genus-specific primers.
Obtained results declared that members of genus Acanthamoeba were detected in 91.7, 83.3, 54.2, 45.8, 12.5 and 12.5% of irrigation water, soil, swabs, wastewater, tap water, and air samples, respectively. The morphologically identified Acanthamoeba species proved to be related to genus Acanthamoeba when tested by PCR. Statistically, the sampling source had a strong significant correlation with the prevalence of Acanthamoeba. The highest appearance of Acanthamoeba was recorded in the spring season for samples from irrigation water, soil, and swabs from surfaces.
In conclusion, the high prevalence of Acanthamoeba species in irrigation water and soil exert public health hazards to students and workers in Helwan University.
https://ejabf.journals.ekb.eg/article_87814_8a6a39790680f55e79a0f7ae673be3dc.pdf
Acanthamoeba
environment
Helwan University
Egypt
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
75
84
10.21608/ejabf.2020.88329
88329
Original Article
Cytochrome Oxidase I (COI) gene analysis of the Nile Puffer Fish (Tetraodon lineatus) from Lake Nasser, Aswan, Egypt.
Medhat H. Hashem
1
Reham M. Abd El-Azeem
2
Hoda Mahrous
3
Ahmed K. Dawood
4
The study of the Nile Puffer Fish (Tetraodon lineatus) from Lake Nasser, Aswan, Egypt is still at a very early stage. Samples from two locations (Khor El Ramla and Tushka East) from Lake Nasser were studied. Partial sequences of the COI gene (barcode) for 20 Nile Puffer Fish from Lake Nasser were successfully amplified and analyzed. The average of nucleotides composition percent of both locations samples was 25.88, 31.54, 24.53, and 18.05 for T, C, A, and G respectively. The base composition analysis of both locations samples of the COI sequence revealed that AT content (50.41%) was higher than GC (49.59%). The current results of the phylogenetic analysis of Lake Nasser samples indicate that there are three groups of Nile Pufferfish in Lake Nasser. Two of them are presented in the south and the last one in the north and south of the Lake. Conversely, the GenBank and Lake Nasser samples indicate that there are two haplotypes from Tetraodon lineatus of the two locations from Lake Nasser based on the partial sequence of the COI gene. The first haplotype includes different samples from the two studied locations (North and south Lake) and the second includes five samples from the North Lake. Finally, the present phylogenetic results for Nile Pufferfish in Lake Nasser (Tetraodon lineatus), together with other African freshwater groups, matched with the monophyletic origin of African freshwater Puffer fishes. As a conclusion, overall data collected from this study will be fundamental for the Lake Nasser fishery and the handling of the problems caused by Nile pufferfish. Therefore, proper utilization of this species towards sustainable management, nutritional composition, and biosecurity issues will lead to achieving a sustainable blue economy.
https://ejabf.journals.ekb.eg/article_88329_aa81100dacd0aff0f9996bed00c4724e.pdf
Tetraodon lineatus
Molecular Phylogenetic
analysis
mtDNA
cytochrome oxidase I
Lake Nasser
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
85
95
10.21608/ejabf.2020.89335
89335
Original Article
Length-weight, condition factor and reproductive aspects of the cobia, Rachycentron canadum (Linnaeus, 1766) in the Arabian Gulf off the United Arab Emirates
Elsayed F. E. Farrag
1
Ahmed M. Alzaabi
2
The present study provides the basic information in the fisheries biology and stock assessment for the cobia, Rachynentron canadum in the Southern part of the Arabian Gulf off the United Arab Emirates. A total of 371 individuals were sampled. The length-weight relationships of R. canadum were estimated as W=0.0022*L3.2536, R2=0.99 for all individuals, W=0.0028*L3.1875, R2=0.98 for males and W=0.0022*L 3.258, R2=0.99 for females. According to b values, the growth type of this species was positive allometric growth (b>3), (t-test: p<0.05). Condition factors ranged from 0.60 to 0.77 for males and from 0.61 to 0.85 for females, while the relative condition factor for males ranged between 0.92 and 1.07 and between 0.96 and 1.14 for females. The monthly calculated mean values of the gonado-somatic index (GSI) of females increased from April, reaching the highest value in May then declined in July. The male/female ratio of this species was 1/1.82. The length at first sexual maturity for R. canadum was estimated at 63.31cm and 75.08cm TL for males and females respectively.
https://ejabf.journals.ekb.eg/article_89335_7b1d69a19d47257c046558961dc4602c.pdf
Arabian Gulf
Cobia
Condition factor
Length-weight relationship
Rachycentron canadum
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
97
107
10.21608/ejabf.2020.89333
89333
Original Article
Population dynamic of Periophthalmodon septemradiatus (Hamilton, 1822) living along the Hau River, Vietnam
Lam T. Tran
1
Quang M. Dinh
2
This study provides the biology of the population of Periophthalmodon septemradiatus, as a basis for species conservation. The result analysis of 3,436 individuals collected on a monthly basis starting from July 2017 to June 2019. Five sites including Long Duc, Long Phu, Soc Trang (LD), An Lac Tay, Ke Sach, Soc Trang (ALT), Phu Thu, Cai Rang, Can Tho (PT), Tan Hung, Thot Not, Can Tho (TH), and Binh Duc, Long Xuyen, An Giang (BD) were conducted along the Hau River from the estuary to the upstream of the river. It showed that the male to female ratio ranged from 1.36:1.00 to 1.87:1.00. Although this species’ L∞ was 12.6 cm for all five populations, the growth coefficient (K) and t0 varied with studied sites. The K value ranged from 0.49 yr-1 (PT) to 1.6 yr-1 (BD), whereas the t0 was -0.12 in BD and -0.43 in PT. Likewise, the longevity of this species was the lowest value in BD (1.88 yr.) and the highest one in PT (6.12 yr.). The total mortality and natural mortality coefficients of fish populations displayed the highest values in the BD (Z=4.11, M=3.14) and the lowest one in PT (Z=1.68, M=1.46). Conversely, the highest and lowest values of the fishing mortalities (F) were in TH (1.58) and PT (0.22), respectively. The species populations were subjected to overfishing in ALT and TH.
https://ejabf.journals.ekb.eg/article_89333_1e69be000bbc18d80e96c8fe89632049.pdf
exploitation rate
mortality
population dynamic
P. septemradiatus
Hau River
Vietnam
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
109
124
10.21608/ejabf.2020.88768
88768
Original Article
Study of the Physico-chemical quality of the Groundwater in the Sidi Taibi, Kenitra, Morocco
Amina El Yemli
1
Nabyl Berrid
2
Youssef A. Idrissi
3
Ali k. Hussein
4
Fuad Al-Nahmi
5
Abdelkhalek Ghazi
6
Water is a vital element for humans. It is important to have it in sufficient quality and quantity, thus ensuring a healthy and safe life in the long term. According to a report by the World Health Organization, five million infants and children die each year from diarrheal diseases due to the pollution of food or drinking water. The main objective of this work is to describe the conditions of supplying and distribution of drinking water through an analysis carried out on the freshwater made available to the inhabitants of Sidi Taibi, Kenitra. To do this, we took the sample from eight wells, aiming to describe the physico-chemical characteristics, so we assess the ecotoxicological contamination by the main heavy metals. The physico-chemical analysis showed that the wells studied have lower concentrations l than the values of the Moroccan standard with the exception of nitrate contents which exceed (55.9-69.2mg/l), concerning the metallic elements, we found only traces. As the parameters follow The conductivity; temperature; pH; cations (calcium, magnesium, potassium, and sodium); anions (chloride, sulfate, bicarbonates, and nitrate); trace elements (Iron, Manganese,..) The consumption of drinking water, a determining factor in the prevention of water-related diseases, should receive special attention. In fact, water intended for human consumption must not contain dangerous chemicals or germs harmful to health.
https://ejabf.journals.ekb.eg/article_88768_690b25ef2deaad506b09a1e2dc933eca.pdf
Well
water quality
Physical parameters Chemical parameters Sidi Taibi
Kenitra
Marocco
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
125
144
10.21608/ejabf.2020.88670
88670
Original Article
GC/MS spectroscopic approach, antimicrobial activity and cytotoxicity of some marine macroalgae from Qusier and Marsa Alam Seashore (Red Sea), Egypt
Doaa A. Abdelrheem
1
Aziz A. Rahman
2
Khaled N. M. Elsayed
3
Sayed A. Ahmed
4
In the present work, three macroalgae viz; Halimeda tuna, Padina gymnospora and Phacelocarpus tristichus, collected from Egyptian Red Sea shores were investigated for their chemical composition and pharmacological properties. Volatile compounds of 70% methanol extracts of three selected algae were analyzed by GC-MS. Antimicrobial and cytotoxic activities of algal extracts (70% methanol) were tested by agar well diffusion and in vitro cell viability assays, respectively. P. gymnospora showed the maximum antibacterial activity against E. coli (13.90±0.66 mm), followed by P. tristichus (12.97± 0.65mm), while H. tuna inhibited significantly the growth of S. aureus (13.17± 0.67mm). Furthermore, the highest antifungal activity was obtained by P. gymnospora, followed by P. tristichus and finally H. tuna against C. neoformas and A. fumigatus. Also,P. gymnospora showed more cytotoxicity against HepG-2 and MCF-7 cell lines than P. tristichus and H. tuna. Moreover, it is the first report of chemical composition, antimicrobial activity and cytotoxicity of P. tristichus and also this research showed new reports on cytotoxicity of P. gymnospora and H. tuna against new cell lines. In conclusion, the Egyptian marine macroalgae possess antimicrobial and cytotoxic activities, that could be investigated for future application in medicine and recognizing novel drugs from the marine resources after checking their bioavailability in vivo.
https://ejabf.journals.ekb.eg/article_88670_53b664f539766d49720a60e7dac7c976.pdf
Halimeda tuna Padina gymnospora Phacelocarpus tristichus GC
MS Antimicrobial activity Cytotoxicity
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
145
156
10.21608/ejabf.2020.89748
89748
Original Article
Species composition, length-weight relationships and condition factor of commercial species grasped by trammel nets and gill nets in the Gulf of Suez, Red Sea, Egypt
Mahmoud A. Saber
1
Amgad M. Shaaban
2
Azza A. El ganainy
3
Hanan M. Osman
4
Three experimental fishing operations were conducted in the Gulf of Suez during October 2018, January 2019, and September 2019 to analyze the species composition of fishes caught by trammel nets and gill nets. Trammel nets and gill nets are important artisanal fishing gears in the Gulf of Suez. The main catch of the trammel net contained 16 species belonging to 11families, on the other hand, the catch of gill net was dominated by 4 species belonging to 4 families. Concerning the trammel net, three species constituted about 89.19 % of the total catch (Gerres oyena 33.87%, Siganus rivulatus 25.32%, and Rhabdosargus haffara 20.0%). While the catch of gill net composed of 4 species Siganus rivulatus (51.69%), Liza carinata (32.17%), Gerres oyena (14.14%), and Diplodus noct (1.99%). The results of the length-weight relationships for 9 species revealed that 6 species have isometric growth, two species namely Pomadasys stridens and Liza carinata showed negative allometric growth whereas one species, Siganus rivulatus has positive allometric growth. The mean condition factor, K for 9 fish species in the Gulf of Suez ranged from 1.0 for Liza carinata to 1.6 for Stephanolips diaspros. The provided data can be used as a basis for proper management of the fisheries of these species in the Gulf of Suez.
https://ejabf.journals.ekb.eg/article_89748_7e566f3442ffdd6285343f5d4cc4075e.pdf
Isometric growth
Gill net
Trammel net
Gulf of Suez
Condition factor
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
157
168
10.21608/ejabf.2020.89755
89755
Original Article
Diet Composition of Gilthead Sea Bream Sparus Aurata from Bardawil Lagoon, North Sinai, Egypt
Samah A. Mokbel
1
Hassanen G. D.I.
2
Nesreen K. Ibrahim
3
Salem M.
4
The diet composition of 609 specimens of Sparus aurata respectively from Bardawil lagoon at three stations, were studied monthly from May 2017 to January 2018. The annual diet composition, monthly variations in the diet composition, the variations of the diet with lengths, and the intensity of feeding were studied. Sparus aurata feeds on a wide variety of prey types; crustacea, mollusks, polychaetes, algae, part of fish, undermined matter, and seagrasses. Crustaceans, mollusks, and polychaetes were the major food items in all months and were found in all length groups for Sparus aurata. Crustaceans and algae increased as the size increased, while mollusks and polychaetes decreased as the fish size increased. The feeding activities of Sparus aurata were quite high in September, December, and January at EL-Nasr, Tulul, and Igzwan stations respectively, and May at EL-Nasr. The finding result revealed that defining the relationships between Sparus aurata with other fishes in Bardawil lagoon, in order to understand the dynamic of this regional ecosystem.
https://ejabf.journals.ekb.eg/article_89755_0af61b0b93348920e83bdb07ee6cd111.pdf
Diet composition
Sparus aurata
North Sinai
Bardawil Lagoon
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
169
180
10.21608/ejabf.2020.92859
92859
Original Article
Hematological variations, histopathology and reversibility of liver function enzymes in post-juvenile Clarias gariepinus exposed singly to five botanical piscicides.
Emeka Ekpendu
ukwadavid@yahool.com
1
Joseph Saliu
2
Adebayo Otitoloju
bayo.otitoloju@gmail.com
3
Uche Ukwa
ukwadavid@gmail.com
4
This study assesses the hematological and histopathological effects of some identified piscicidal plants on post-juvenile Clarias gariepinus. 1/10LC50 values of each of the five aqueous extracts and ammonium solution were used for the 28-day sub-acute test. The fishes were exposed for 14 and 28 days and on the 28th day, the group was allowed to recover. and RBC, WBC, as well as PCV were estimated using electronic counters. The activities of the liver enzymes; ALT, AST, and ALP were determined for exposure and recovery. Gill and liver alterations after exposure were estimated for each of the extracts. There was a decrease in RBC and PVC from the 14th day and 28th day exposure in all the extracts. A. occidentale had the lowest RBC level after 28 days of exposure. ALT increased after recovery for all the extract except for N. tabacum. ALP increased after recovery only for A. occidentale. A. occidentale and N. tabacum showed the highest liver and gill alterations with high frequencies of severe vacuolization, inflammation, degeneration, and necrosis with alteration index of 2.44 and 3.33 respectively.
https://ejabf.journals.ekb.eg/article_92859_e1f9bcb945c5d69332fda5b9c77a5607.pdf
Clarias gariepinus Hematology
histopathology
Reversibility
Botanicals
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
181
191
10.21608/ejabf.2020.89737
89737
Original Article
Isopod parasites in the Egyptian fisheries and its impact on fish production: Lake Qarun as a case study
Sahar Fahmy Mehanna
1
Parasitic infestation is a cosmopolitan phenomenon in almost all the habitats, especially among the aquatic biota. Egypt derives its fish production from two resources; natural (marine and inland waters) and aquaculture. In recent years a serious decline in fish production from natural resources was recorded and a number of challenges were identified from which overfishing, illegal fishing techniques, pollution, habitat degradation…etc. Five years ago, a new challenge appears on the surface and affected dramatically the fish production and quality in some fishery resources in Egypt, the isopod parasites. Although isopod parasites were observed in marine fisheries for a long time and considered as a part of the aquatic ecosystem, today it received considerable attention because they cause serious damage to our fishery resources especially in one of the largest inland lakes in Egypt, lake Qarun. Isopods associate with many species of commercially important fishes in the lake causing significant economic losses in commercial fish species ending by loss more than 70% of the lake catch. This study will throw the light on the isopod parasites in lake Qarun with their impacts on the fishery resources as well as propose some practical solutions to overcome this serious problem in Egypt.
https://ejabf.journals.ekb.eg/article_89737_655b2c824daf32c6da6268273efa5a16.pdf
Fishery resources
Isopod parasites
Lake Qarun
Egypt
Catch statistics
Management
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
193
212
10.21608/ejabf.2020.91881
91881
Original Article
The influence of environmental factors on the seasonal dynamics of Cladoceran community in the Oubeira Lake (Algeria)
Nadira Sehili
1
Salah Arif
2
Hassen Touati
touati-hassen@hotmail.com
3
Mourad Bensouilah
4
This study aims to inventory Cladocerans communities and determine their seasonal distribution according to certain environmental parameters in a natural shallow freshwater lake (Lake Oubeira) that El Kala National Park shelters. The values of chlorophyll-a and transparency observed during the study period indicate that the water body fluctuated between eutrophic and hypereutrophic status. Occurrence frequency of the 17 listed species revealed the dominance of Bosmina longirostris and the constancy of Ceriodaphnia lacustris. Among the 15 remaining species, 9 are regular (Oxyurella tenuicaudis, Alona guttata, Alonella exigua, Pleuroxus aduncus, Eubosmina coregoni, Ceriodaphnia rotunda, Moina brachiata, Diaphanosoma brachyurum, Diaphanosoma lacustris), 4 are incidental (Scapholeberis mucronata, Acroperus elongatus, Simocephalus serrulatus, Moina micrura) and 2 are rare (Macrothrix rosea, Sida crystallina). The number of species encountered in Oubeira waters is around 7, 17, 16, and 14 in spring, summer, autumn and winter respectively. In terms of abundance, the 7 species collected in the spring account for more than 88% of the total number of individuals collected. B. longirostris represents 9/10 of the overall density recorded by the Cladocerans collected. This species is highly abundant in spring (94% of its overall density) and correlated positively with transparency. C. lacustris, D. brachyurum, M. micrura, M. brachiata are absent in spring and are mainly present in summer and autumn; these species show positive correlations with water temperature, chlorophyll-a, nutrients and negative with transparency. It is also interesting to study the temporal dynamics of the various zooplanktonic groups and their interaction with phytoplankton and the fish inhabiting water bodies. Such studies need to be continued because they allow the creation of databases that will be used in biomonitoring programs and management of continental waters.
https://ejabf.journals.ekb.eg/article_91881_1dde91d120c50a65d9ac9b519d0a906f.pdf
fresh water
Cladocerans
environmental variables
Oubeira Lake
Seasonal dynamics
Algeria
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
213
223
10.21608/ejabf.2020.90301
90301
Original Article
Production of Lignin Peroxidase from Aquatic Bacteria, Alcaligenes aquatilis
Nashwa M. H. Rizk
1
Ayman S. Eldourghamy
2
Samar A. Aly
3
Shawky Z. Sabae
4
Ahmed Sobhy
5
The main objective of the present work was to decolorize dye-containing wastewaters of textile industries using dye decolorizing bacteria which isolated from different aquatic samples. The most potent bacterial isolate capable of decolorizing malachite green with a decolorization rate of 97% within 48 h under aerobic conditions was selected for phylogenetic identification. The 16S rRNA amplification revealed that the isolate DB 8 was highly related to Alcaligenes aquatilis by 100%. The activity of lignin peroxidase responsible for malachite green decolorization was detected. The culture conditions for lignin peroxidase production by Alcaligenes aquatilis were optimized using response surface methodology. The optimal concentrations obtained were soluble starch, 10.2 (g/l); ammonium nitrate, 5.0 (g/l); KH2PO4, 2.0 (g/l); inoculums size, 10%; and time course, 36.6 (h) with optimized LiP activity of 5.6 U/ml.
https://ejabf.journals.ekb.eg/article_90301_e4a5eaf032549d100dbc051bf3070b11.pdf
Wastewater
aquatic bacteria
Lignin peroxidase
Alcaligenes aquatilis
Decolorization
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
225
243
10.21608/ejabf.2020.89905
89905
Original Article
Drivers of change in the epifaunal assemblages associated with intertidal macro-algae at the Mangrove site south Safaga, Egypt, Red Sea
Walaa M. Shaban
1
Salah E. Abdel-Gaid
2
The present study aimed to study the effects of intertidal habitat complexion on algae-epifaunal communities in the poly-zoned coastal mangrove site south Safaga on the Egyptian Red Sea coast. The study focuses on the effects of main intertidal primary habitats along with the facilitation of habitat cascades on the focal/inhabited organisms, which represented here by algae-epifaunal organisms. The intertidal main primary zones (habitats) in the study area including 1- outer mangrove tree zone, 2- inner flat back reef area, and 3- A sand lagoon in between the previous two zones. However, different algae species that either stand-alone or mixed by other algae species in patches were considered here as secondary habitat-formers.
Results indicated that a total of 64 epifaunal species were recorded in different intertidal macro-algae habitats and such faunal diversity and abundance affected mainly by the primary habitat in which approximately 50% of the current epifaunal species showed specificity to only one primary habitat. Results also detected that habitat facilitation was strongest when the secondary habitat-former was most functional for the epifaunal organisms in feeding and hiding from predators. The present study suggested that such effects of secondary habitat formers on the algae-epifaunal diversity are depending essentially on the primary habitat construction and its zone characteristics. In consistence, the results suggested that predation effort is the main factor controlling the distribution of algae-associated organisms depending on how far such organisms can benefit from the special shape and construction of each algae habitat-unit in protection and hiding from predators.
https://ejabf.journals.ekb.eg/article_89905_68a11795792ed574df44275c6a01e667.pdf
Epifaunal assemblage
algae
Mangrove
Red Sea
Habitat complexity
Habitat cascade
diversity
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
245
255
10.21608/ejabf.2020.91832
91832
Original Article
Sub-chronic Exposure to Magnesium Hydroxide Nanoparticles Alters Some Biochemical Parameters in Clarias gariepinus
Bukola T. Aluko
1
Omotade I. Oloyede
2
Ebenezer T. Adebayo
3
Adejoke A. Adewumi
4
Elijah O. Odesanmi
5
Yemisi R. Alli Smith
6
Oladimeji S. Tugbobo
7
Magnesium hydroxide nanoparticles (MgOHNPs) have gained commercial interest in the areas of waste remediation and recycling of wastewater for portable use. MgOHNPs is among the least investigated nanoparticle and thus its toxicological effects is yet to be unveiled. Clarias gariepinus were exposed to 62.5, 125, and 250 mg/L suspensions of small size (10 nm) MgOHNPs for 21 days under semi-static conditions. Thereafter, they were sacrificed and alterations in the hematological parameters, and some biochemical indices were examined. The nanoparticle was characterized by UV/Vis spectrophotometry, and Fourier transform infrared spectroscopy (FTIR). The water quality parameters such as chloride ions and dissolved oxygen significantly decreased, while pH, total alkalinity, carbon (IV) oxide, total hardness, and nitrate increased significantly in the exposed media compared to the control medium. The results of this investigation revealed a concentration-dependent increase in the plasma levels of albumin, total protein, magnesium, calcium, chloride, alkaline phosphatase, and acid phosphatase. Whereas, the levels of aspartate aminotransferase, alanine aminotransferase, glucose, total bilirubin, potassium, and creatinine decreased significantly (𝑃 < 0.05). The hematological parameters remained unaltered in the fish at the tested doses. The results indicated that exposure to MgOHNPs could lead to disturbances in blood biochemistry of C. gariepinus
https://ejabf.journals.ekb.eg/article_91832_8a48b009cf035f4931190b8ee863e7d3.pdf
sub-chronic
exposure
Nanoparticles
Magnesium hydroxide
Clarias gariepinus
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
257
270
10.21608/ejabf.2020.90502
90502
Original Article
Seasonal variations of heavy metals in the marine bivalve Cerastoderma glaucum (Bruguière, 1789) from Temsah Lake, Ismailia, Egypt, and their relation to antioxidant enzymes
Salwa A. El-Saidy
1
Gihan M. El-Khodary
2
Nahla E. Omran
3
Karolin K. Abd El-Aziz
4
Mohamed H. Mona
5
Cerastoderma glaucum is a common bivalve in Temsah Lake. It plays important direct and/or indirect roles in the nutrient cycle. This work determined the seasonal variation of heavy metals [copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), zinc (Zn), chromium (Cr), nickel (Ni), cobalt (Co), cadmium (Cd) and lead (Pb)] in the tissue of the investigated bivalve, and the effect of these metals on the antioxidant enzymes. The results revealed that the greatest values for all studied metals in the soft tissues of C. glaucum occurred during winter and autumn seasons, while the least values were recorded during spring and summer seasons. Mg and Fe concentrations were significantly higher in all seasons when compared with all other metals. The concentrations of metals, such as Mn, Ni, Fe, and Cr were present beyond the WHO recommended standards, especially during winter and autumn seasons. Also, this study showed highly significant increase in lipid peroxidation (MDA) activity during autumn and winter seasons that was associated with a decrease in the mean activity levels of catalase (CAT) and glutathione peroxidase (GPx) than summer season and decrease in superoxide dismutase (SOD) activity than spring season. These results related to high heavy metals concentrations, such as Cu, Fe, and Zn during the autumn season, and Mg, Mn, Cr, Co, and Ni during the winter season. Moreover, the present study recorded positive significant correlations between MDA activity and Cu, Cr and Fe concentrations, and significantly negative correlations between MDA activity and SOD activity, between CAT activity and Ni and Mn, and between GPx and Mn, Cr and Fe. So, the present study suggests that MDA and antioxidant enzymes (CAT, GPx and SOD) can be used as biomarkers to reflect the level of heavy metal pollution stress, especially Fe and Cr in the soft tissues of C. glaucum.
https://ejabf.journals.ekb.eg/article_90502_9640e3b3e6ad67d389054e55bd50fed0.pdf
Cerastoderma glaucum
Temsah Lake
Heavy metals
Lipid peroxidation
Antioxidant enzymes
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
271
283
10.21608/ejabf.2020.91119
91119
Original Article
Larvicidal, biochemical and physiological effects of acetamiprid and thiamethoxam against Culex pipiens L. (Diptera: Culicidae).
Doaa R. Abdel-Haleem
1
Abir A. Gad
2
Shaimaa M. Farag
3
The resistance of mosquitos to conventional insecticides created an urgent need for using alternative insecticides. In this study, the toxicity of the neonicotinoids (acetamiprid and thiamethoxam) was evaluated against the 3rd larval instar of Culex pipens. In the larvicidal assay, acetamiprid and thiamethoxam showed promising larvicidal activity against Cx. pipiens, with LC50 values of 0.0093 and 0.0305 ppm after 24 hrs, 0.0078 and 0.0206 ppm after 48 hrs and 0.0065 and 0.0137 ppm after 72 hrs of insecticidal exposure. The activity of acetylcholinesterase (AChE), carboxylesterase, (α-esterase), (β-esterase), and glutathione S- transferase (GST) were determined after 72 hrs of insecticidal exposure. Acetamiprid showed a significant increase in the activity level of AChE, GST, carboxylesterase, α, and β- esterases than thiamethoxam. The hemocytes of the treated 3rd larval instar of Cx. pipens were sensitive to both insecticides and showed remarkable deformation compared with control. Therefore, both detoxification enzymes and physiological resistance play a great role in neonicotinoids detoxification.
https://ejabf.journals.ekb.eg/article_91119_259ee950d3e1825ec28a9c9d2156bdb1.pdf
Culex pipens
Acetylcholinesterase
carboxylesterase
hemocyte
Acetamiprid
thiamethoxam
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
285
298
10.21608/ejabf.2020.91650
91650
Original Article
Applying a cultured Brachionus plicatilis crude extract as a novel source of natural medical bioactive compounds
Moustafa S. Abdelhameed
1
Mohamed R. Fishar
2
Magdy T. Khalil
3
Mahmoud H. Hegab
4
Hosam E. Elsaied
5
Ihab K. Mohamed
6
Hesham R. A. Mola
7
Brachionus plicatilis is the most common rotifer species that has been cultured for fish farming. However, all applicable studies of B. plicatilis have been concerned about its nutritional values in aquaculture; while there is little attempt to use it as a potential source for medical bioactive substances. Therefore, the study aimed to culture the rotifer B. plicatilis in sustainability with Cyclotella sp. (as feeds), to applying its extractions as antitumor, antimicrobial, and antioxidant. B. plicatilis crude had a significant impact on the cell growth inhibition of MCF-7 cells (breast cancer), where the maximum cell growth inhibition (91.53 %) was detected with 10 mg/ml of the extract. On the other hand, the Gram-positive organisms (Staphylococcus aureus, Streptococcus mutans and Methicillin-Resistant Staphylococcus aureus) were moderately sensitive for the B. plicatilis extract, and their inhibition zones (16, 13 and 11 mm, respectively) were smaller than that (24, 20, and 15 mm, respectively) produced by gentamycin (control). Otherwise, the activity of B. plicatilis extracts against Gram-negative organisms was zero except with Salmonella typhimurium, which produced a very small inhibition zone (8mm). Also, the rotifer B. plicatilis extract showed antioxidant activity, but their IC50 value was larger than IC50 that belongs to ascorbic acid, which means that the antioxidant ability of ascorbic acid is stronger than B. plicatilis crud extract sample. Therefore, B. plicatilis crud extract may have a promising future in the treatments of many diseases, including cancer, and bacterial infection.
https://ejabf.journals.ekb.eg/article_91650_36fe39c229365308a1afb0c506d71543.pdf
Natural product
Zooplankton
Aquatic invertebrates Antitumor
Antimicrobial
antioxidant
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
299
309
10.21608/ejabf.2020.92401
92401
Original Article
Parasitological assessment of the purifying performance of the wastewater station by natural lagoon of Azilal, Morocco.
Youssef A. Idrissi
1
Dalale Mansouri
2
Amina El Yemli
3
Imane Kherrati
4
Nabyl Berrid
5
El Hassan Ghachoui
6
Ali k. Hussein
7
Khadija El Kharrim
kharim@gmail.com
8
Driss Belghyti
belghyti@hotmail.fr
9
In the context of saving human health against waterborne diseases, the present work is interested in the study of some parasitological characteristics of raw and treated wastewater from the treatment station of Azilal city. The purpose of the analysis is to determine the parasite load, hence the abatement of the station, the samples analyzed were taken between August 2013 and May 2014, aimed to detect the presence of eggs of some parasites, the examination of the latter was based on the technique of Bailenger. Furthermore, for raw wastewater at the station entrance, the results revealed the presence of Helminth’s eggs with a load of 39.3 eggs/l. The phylogenetic study isolated three different classes: Nematodes, Cestodes, and Strongles respectively with a load of 27.7, 7, and 4.6 eggs/l. However, the distribution of these collected eggs shows that the Nematode class is most represented with five species, followed by the Cestodes class with three species. For treated wastewater at the station exit, we isolated Helminth’s eggs at an average concentration of 3.6 eggs/l of which 2.7 eggs/l correspond to Nematode, 0.5 eggs/l at Cestodes, and 0.4 eggs/l at Strongles. In light of these results, it can be said that the station does not ensure the total elimination of Helminth’s eggs. These results remain far from those encountered in the literature.
https://ejabf.journals.ekb.eg/article_92401_2558388023a317ea71e657f650e00c00.pdf
Parasitology
Treatment
Station
Wastewater
Helminth eggs
Azilal
Morocco
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
311
322
10.21608/ejabf.2020.92251
92251
Original Article
Origin of Invasive Fish Species, Peacock Bass Cichla Species in Lake Telabak Malaysia Revealed by Mitochondrial DNA Barcoding
Aliyu G. Khaleel
1
Syafiq A. M. Nasir
2
Norshida Ismail
norshida@unisza.edu.my
3
Kamarudin Ahmad-Syazni
4
Peacock bass (Perciformes, Cichlidae, Cichla)are multi-coloured and highly predatory fish originated from the Amazonian region. The species was deliberately introduced into Malaysia freshwater bodies by anglers in the early 1990s for sport fisheries. In this recent study, we found the population of peacock bass in Lake Telabak, a man-made lake in Besut, Terengganu. Using the mitochondrial DNA analysis approach, the origin and taxonomy of peacock bass in the lake were clarified. A total of forty fishes were sampled from Lake Telabak for the analysis. Haplotype was detected among all samples. The current study revealed that Cichla spp. in Lake Telabak are closer to Cichla ocellaris (Bloch and Schneider, 1801) with the highest sequence similarity of 99.84% as blasted at the National Center for Biotechnology Information (NCBI) database. The evolutionary history inferred using the maximum likelihood method in the molecular phylogenetic analysis also confirmed the species as C. ocellaris. The origin of Cichla spp. in the current study was traced to the Amazonas and Parà states of Brazil (Manaus, Novo Airão, Tapajós, and Tefé).
https://ejabf.journals.ekb.eg/article_92251_0838ede800e25291a7c91487dbd5491a.pdf
Peacock bass
invasive species
mitochondrial DNA barcoding
Lake Telabak
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
333
349
10.21608/ejabf.2020.91939
91939
Original Article
DNA barcoding and comparative genetic diversification among species of family Sparidae in the coastal waters of Egypt
Nermin Ibrahim
nanasemsem2004@yahoo.com
1
Eman M. Abbas
2
Ayman El-Seedy
el_seedyus@yahoo.com
3
Taha Soliman
tahasoliman2000@yahoo.com
4
Fawzia S. Ali
5
Species of family Sparidae, commonly called, sea breams, are widely distributed from temperate to tropical waters and are of great economic interest. However, in Egypt, limited data is available on genetic variation and evolutionary relationships of family Sparidae. Therefore, the study of the genetic diversity among sparid species is crucial for proper management and convenient strategies. The aim of this study was to evaluate the genetic diversification among 22 species belonging to family Sparidae from the Mediterranean Sea and the Red Sea in Egypt using three different molecular markers. DNA barcoding, using Cyt-b mitochondrial gene, was applied as an initial step for species identification and diversification. In addition, Randomly Amplified Polymorphic DNA (RAPD), and Inter Simple Sequence Repeat (ISSR) markers were employed in estimating the genetic diversity among the studied species. Based on each molecular marker, a phylogenetic tree was constructed for the studied sparid species according to the calculated genetic distance/ similarity. DNA barcoding using mitochondrial Cyt-b gene provided efficient DNA barcodes for most of the studied species. RAPD assays (using 20 RAPD primers) produced a total of 308 bands, of which 91.8% were polymorphic. Eight ISSR primers amplified a total of 197 bands, 97.9% of which were polymorphic. RAPD and ISSR profiles differed substantially among the 22 sparid species, enabling easy discrimination. However, ISSR had a higher power of discrimination compared to RAPD markers. The constructed phylogenetic trees based on the employed molecular markers provided the update for the barcoded Sparidae species evolution. The employed molecular markers in this study were efficient in species genetic diversification. Cyt-b was successfully utilized in species barcoding, whereas, the combination of RAPD and ISSR-PCR profiles provided substantially differentiated profiles for each species, which makes them suitable for measuring genetic divergence among species.
https://ejabf.journals.ekb.eg/article_91939_460fea6d410c5f3462a0cbec4ba630e8.pdf
Sparidae
Genetic diversity
RAPD
ISSR
Mitochondrial Cyt-b
Red Sea
Mediterranean Sea
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
351
364
10.21608/ejabf.2020.92252
92252
Original Article
Comparative Analysis of Morphometric Characteristics and Mucous Cell Distribution between Pangasius hypophthalmus and Clarias batrachus
Paul Van Siang Lian Mang
1
Wannee Jiraungkoorskul
wannee.jir@mahidol.ac.th
2
The morphology characteristics and the mucous cell distribution in two species of commercial catfish (Pangasius hypophthalmus and Clarias batrachus) were investigated in the present study. To study morphological characteristics, the parameters - such as total length (TL), standard length (SL), body depth (BD), pre-dorsal length (PDL), pre-pectoral length (PPCL), pre-pelvic length (PPVL), pre-anal length (PAL), depth of caudal peduncle (DCP), length of caudal peduncle (LCP), head length (HL), eye diameter (ED), caudal fin length (CFL), pectoral fin length (PFL), and length of dorsal fin base (LAFB) were measured. The mucous cell distributions of the dorsal and ventral parts in the head, abdomen, and GI tract were examined using periodic acid Schiff (PAS) and hematoxylin and eosin (H&E) staining dyes. In P. hypophthalmus, the tail region had the highest mucous cell count, followed by the abdomen and the head, whereas in C. batrachus, the pattern was the opposite. In conclusion, P. hypophthalmus was longer than C. batrachus in most morphological characteristics. The dorsal region had more mucous cells in both species than the ventral region. Overall, C. batrachus was found to have more mucous cell distribution in both ventral and dorsal parts compared to P. hypophthalmus, suggesting that C. batrachus has a better innate immune system than P. hypophthalmus. The sizes of blood cells were also found to be different in both species.
https://ejabf.journals.ekb.eg/article_92252_8e2d12f6767915107d0c1cc66aea8806.pdf
catfish
dorsal part
H&E
PAS
ventral part
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
381
388
10.21608/ejabf.2020.92321
92321
Original Article
Growth Performance of Cultivated Spiny Lobster (Panulirus homarus, Linnaeus 1758) in Tuban, East Java, Indonesia
Ikmalia Amali
ikmalia08a@gmail.com
1
Putri Desi Wulan Sari
2
Spiny lobster is one of the potential and important economic fishery commodities. The demand for spiny lobster consumption continues to increase from year to year. Therefore, it is necessary to develop aquaculture efforts to fulfill the demand for lobster. The aim of this research is to observe the growth performance of cultivated spiny lobsters. The working method used in this study was data collection methods, consisting of primary data obtained from direct observations and secondary data obtained from interviews and literature study. The primary data included measurement of water quality, carapace length, Specific Growth Rate (SGR), absolute length growth, and Survival Rate (SR). Secondary data included spiny lobster aquaculture activity obtained from the literature and journal from the previous study.
There are several technical activities of spiny lobster (Panulirus homarus) grow-out, which include pond preparation, fry supply, lobster stocking, feeding, measuring water quality, siphoning, and sampling to measure the growth of spiny lobster. The grow-out process of spiny lobster in this study is started from the average length of 10-20 cm, carapace length of 4.05, and weight size of 145.69 gram. The feed that given to the grow-out activity is trash fish and green clams. The parameters of growth measurement include total length, carapace length, and body weight. After reaching harvest size (one-month) of the rearing process, the value of SGR reached 0.15%, 0.5 cm in absolute length growth, and 97% in survival rate.
https://ejabf.journals.ekb.eg/article_92321_a0b7d39a7d1af43d8d075ef1a3207558.pdf
Aquaculture. Growth performance. Spiny lobster. Panulirus homarus. Tuban
East Java
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
389
401
10.21608/ejabf.2020.92310
92310
Original Article
Genetic divergence and phylogenetic relationship among three species of Mullet inferred from RAPD markers in Egypt
Hesham A. Hassanien
1
Ramadan M. Abou Zied
2
Kamal A. Eldeeb
3
The present study was carried out to determine the genetic divergence and phylogenetic relationship for three species of mullet (Mugil cephalus, Liza ramada, and Valamugil seheli) in Egypt using random amplified polymorphic DNA (RAPD) markers. two hundred and twenty-five specimens were randomly collected from Qarun, Manzala, and Burullus lakes and some commercial farms neighboring each of the three lakes. The results indicated that the highest genetic variation of 37.08% in Liza ramada from lake Manzala and the lowest difference genetic variation of 24.97% in Mugil cephalus from lake Manzala were observed. Dendrogramshowed the highest genetic similarity between Liza ramada lake Qarun and surrounding farms. It could be concluded that Liza ramada lake Manzala is best to work for selection programs. There is a need for further genetic studies to give a more complete picture of mullet in Egypt.
https://ejabf.journals.ekb.eg/article_92310_91f252fe3e36cf554771018da23e4777.pdf
Mugil cephalus
Liza ramada
Valamugil seheli
genetic variation
Random Amplified Polymorphic DNA
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
403
415
10.21608/ejabf.2020.92320
92320
Original Article
Genetic diversity and phylogenetic reconstruction of grouper (Serranidae) from Sunda Land, Indonesia
Sapto Andriyono
sapto.andriyono@fpk.unair.ac.id
1
Adrian Damora
adamora@unsyiah.ac.id
2
Andi A. Hidayani
3
Groupers are coral reefs associated and favored in the aquaculture industry. Aquaculture system has been successfully carried out with a floating cage system as well as in an aquaculture system in a pond. Lately, breeding activities in groupers have produced hybrid species that are expected to increase production and fulfillment of the demand of groupers in the live fish market in Singapore, Taiwan, and China. The study of biology has supported the success of many of groupers, but information on genetic variation in commercial groupers is still very limited. This report is a preliminary study of genetic diversity in commercial groups from Java, Bali, and Aceh, which is the Sunda land region in western Indonesia. Eleven specimens have been identified with the barcode region (Cytochrome c Oxidase subunit I gene region). As a result, two species were identified as Indonesian haplotypes, namely Epinephelus merra and Cephalopholis cyanostigma. Phylogenetic tree analysis produces two large clades, namely Clade I (Epinephelus) and Clade II (Cephalopholis and Variola), which are clearly separated in the subfamily Epinephelinae. However, in-depth studies of genetic variation need to be more accurate by increasing the number of specimens from various regions in Indonesia to describe genetic diversity comprehensively.
https://ejabf.journals.ekb.eg/article_92320_7d2ba965e84313a761dfa9b4e927abbd.pdf
Commercial groupers
Serranidae
Genetic study
Sunda land
Indonesia
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
417
424
10.21608/ejabf.2020.92405
92405
Original Article
Studies on the infestation of Red Sea cultured Black Sea bream (Spondyliosoma cantharus ) with Caballerocotyla sp. (Monogenea; Capsalidae) parasite
Abeer E. Mahmoud
1
Hussien A. Osman
2
Mona S. Zaki
3
Alla Z. Abubrika
4
The Monogenea (Class: Platyhelminthes,) are common parasites of the skin and gills of both marine and freshwater fish. This study aimed to the assessment of the prevalence percent, seasonal prevalence, intensity of parasitism, clinical and post mortem lesions with an estimation of organic materials in the farm water of infested cultured Black Seabream (Spondyliosoma cantharus)with capaclidae sp. in a private fish farm in the Gulf of Suez, Egypt. The study was performed on 240 Black Seabream of body weight ranged 250-1000 gm. Survey on cultured marine fish was performed periodically each season for one year. Clinical and post mortem, parasitological, total and seasonal prevalence examination, determination of ammonia, nitrite, and nitrate in cultured water and determination of physicochemical parameters of water during different seasons. Infested Spondyliosoma cantharus fish appeared with dark colour, suffered from respiratory distress; increase of opercular movements, with opening mouth, abnormal swimming, and flashing. Hemorrhages at the abdominal region, the caudal fins were observed. Scales sloughing with shallow wounds at different regions of the body and congestion of gills were noticed. The isolated parasite is subclass: Monogenea, order: Monopisthocotylea, Family: Capsalidae, Genus: Caballerocotyla. The concentration of organic matter was closely related to the prevalence of Caballerocotyla sp. From the present study, it was concluded that Caballerocotyla sp. (Monogenea; Capsalidae) parasite isolated from the Red Sea cultured Spondyliosoma cantharus fish in a private fish farm in the Gulf of Suez, Egypt. The prevalence of ofCaballerocotyla sp. was closely related to the concentrations of organic matter (ammonia, Nitrite, and Nitrate).
https://ejabf.journals.ekb.eg/article_92405_50208039c8f04642241b71cd6ce74a1e.pdf
Black Sea bream Spondyliosoma cantharus Caballerocotyla
organic matter
Gulf of Suez
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
425
438
10.21608/ejabf.2020.92403
92403
Original Article
Nitrogen and protein contents of Ulva fasciata and Corallina officinalis under environmental variations.
Wagdy Labib
wagdysea@hotmail.com
1
Shimaa Hosny
2
The study estimates temporal and regional variations of nitrogen and protein content of the green algae Ulva fasciata and the red Corallina officinalis collected at three sites of the different entities along the Alexandria coast. The heterogeneity in environmental conditions contributes significantly on the variation of the algal nitrogen and protein contents. However, the varied storage of nitrogen by the algae cannot be directly linked to the ambient dissolved inorganic nitrogen concentrations. Ulva fasciata can accumulate higher concentrations than Corallina officinalis. The study suggests a new nitrogen-protein conversion factor to be applied for each species. A comparison with other published data is discussed.
https://ejabf.journals.ekb.eg/article_92403_37909b201f590d0e434ecc602c7fac13.pdf
nitrogen
protein
Ulva fasciata
Corallina officinalis
Conversion factor
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
451
462
10.21608/ejabf.2020.92865
92865
Original Article
Biodiesel Production, Characterization and Biochemical Variability by Microalga Nannochloropsis oculata under Stressed Culture Conditions
Hanan M. Khairy
1
Hawazin H. Mutawie
2
Heba S. El-Sayed
3
Nayrah A. Shaltout
4
Microalgae are recognized as a promising source for biofuel production, which is known as a renewable source of energy. This study was conducted to optimize the biodiesel production quantitatively and qualitatively by the microalga, Nannochloropsis oculata upon culturing on two different culture media (F/2 and Boussiba media) as a comparative study through stress in the main culture compositions by 1) Treating with different salinity concentrations (20, 40 and 60psu), 2) Depleting from phosphorus (P) and nitrogen (N) sources for the two media. After different treatments, the growth rate, protein, carbohydrate, lipid, and fatty acids contents were determined. The algal analysis cleared that increasing salinity to 60psu in F/2 medium and 40psu on the Boussiba medium led to increasing the number of N. oculata cells with the maximum protein and carbohydrate contents at late exponential phase. At lower salinity, there were no significant differences in total protein content while carbohydrate contents showed slight variations, and its minimum values were obtained at (N) starved medium. Total lipid and total fatty acids content were higher in N. oculata cultured on F/2 medium than on Boussiba medium at different treatments and achieved its maximum when cultured on N-starved > P-depleted > Salt-stressed 60psu, respectively. The highest physical properties of the produced biodiesel represented in the degree of unsaturation (SD), iodine values (IV), Cetane number (CN), and oxidation stability were recorded in N. oculata cultured on higher salinities and N-depleted conditions in the two media. Using higher salinities up to 40psu (available in the natural seawater), N-depletion or P-depletion in F/2 medium for achieving highly economical results in the production of N. oculata microalga. Biodiesel produced from N. oculata oil can be considered as a potential candidate for commercial sources.
https://ejabf.journals.ekb.eg/article_92865_a120797c7d104eef4eee3e0bd6ecac0d.pdf
salt stress
nitrogen
phosphorus
Starvation
Biodiesel
Nannochloropsis oculata
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
463
480
10.21608/ejabf.2020.94043
94043
Original Article
Digentetic Trematodes in Dicentrarchus labrax cultured in Egypt: Prevalence, Clinical Features, Body Condition, and Histopathology
Salah M. Aly
1
Sayed Abou El-Gheit
2
Amr Fadel
fadel_aqua@yahoo.com
3
Habiba Essam El-Din
4
The current work aimed to study the types and seasonal occurrence of digenetic trematodes in D. labrax cultured in Egypt. A total number of 1800 D. labrax, (150/season- 600/farm) of average weight 95.14±10.37 gm were collected from three Egyptian farms during 2019. Four digeneans were isolated from the gastrointestinal tract; Metadena crassulata Derogenes varicus, Acanthostomum spiniceps, and Diplomonorchis sphaerovarium. The highest infection rate was detected in Farm 2 (30.16 %), specifically at spring (27.76% ), where the range of water temperature was 21.12- 24.63 oC, and salinity 15.85-32.34 ppt. The infected fish were apparently healthy, except in heavy infestation where body weakness and anemia of the internal organs were evident. The infected fingerling recoded the lowest condition factor (0.98) and mean parasitic intensity of 3.9, but higher values were noticed in juvenile, adult, and broodstock. Moreover, weak linear regression was reported between infection intensity and weight (R=0.91), fish length (R=0.964), and condition factor (0.956). Remarkable pathological alterations were observed in the intestine of infected fish, including edema, aggregation of inflammatory cells, mucinous degeneration and sloughed necrotic areas. Therefore, preventive and control measures are recommended to avoid such infections that potentially influence the health status and productivity of infected fishes
https://ejabf.journals.ekb.eg/article_94043_11f9d02f5dded1c8df346dcc9b161fe3.pdf
Dicentrarchus labrax
Digenetic trematodes, Body condition Prevalence, Histopathology
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
323
332
10.21608/ejabf.2020.95329
95329
Original Article
Distribution and abundance of Cerithideopsilla djadjariensis (Martin 1899) (Potamididae) on Avicennia marina in Rembang, Central Java, Indonesia
Dafit Ariyanto
dafitariyanto676@gmail.com
1
Dietriech G. Bengen
2
Tri Prartono
triprartono@yahoo.com
3
Yusli Wardiatno
wardiatno@gmail.com
4
Mangrove forests are known to provide important ecosystem goods and services such as nursery areas for several important aquatic species, and a variety of food for several animal groups, including gastropods. This research was conducted from September 2016 - August 2017. The aim of the research was to determine the spatial and temporal pattern distribution of C. djadjariensis based on mangrove zone, environmental characteristics, and organic materials. The gastropod samples were taken by using a square transect sized 25 cm x 25 cm. The spatial analyses were done by using transect 10 m x 10 m. The gastropod and environmental characteristics were analyzed using Principal Component Analysis (PCA). The total of gastropod C. djadjariensis was 528 ind./m2. The result of high significant was organic matter (0.92), total phosphorus (0.92), dust (0.86), clay (0.86), temperature (0.69). The distribution and abundance are affected by the availability of food, water condition, and zone in the mangrove.
https://ejabf.journals.ekb.eg/article_95329_f01aae9c3073f375421f05db88aa7157.pdf
C. djadjariensis
Avicennia marina
correlation
Gastropod
Mangrove
physico-chemistry
Rembang
eng
The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)
Egyptian Journal of Aquatic Biology and Fisheries
1110-6131
2536-9814
2020-05-01
24
3
365
379
10.21608/ejabf.2020.98391
98391
Original Article
Morphological study of Sudanonautes africanus (Miline-Edwards, 1869) in three freshwater bodies of Ondo State, Nigeria.
Omoniyi M. Popoola
1
Faith E. Udoh
2
Freshwater crabs are a cheap and important source of animal protein for human beings. They also serve as bio-indicators of ecosystem disruption and also conservation icons. Despite their importance, there are still some unresolved issues in their phylogenies. This study aimed to examine the morphological variations in freshwater crab, S. africanus from three different water bodies in South-west, Nigeria. Thirty samples were collected from each of the study locations morphological study using morphometrics and meristics characters. Morphometrics characters did not show variations in species of crabs, unlike meristic that showed variations in the episternum and abdominal segments of S. africanus. The study also revealed that the crabs exhibit a negative allometric growth (b<3). The condition factor revealed that the crabs are healthy (K>1). Cluster diagram of the Canonical Variate analyses of the morphological parameters showed that there are no variations in the morphological characteristics of S. africanus. Cluster diagram of the meristic characters shows that there are little variations in the meristic characters of S. africanus in the three study locations.
https://ejabf.journals.ekb.eg/article_98391_abd442247467931234b5300b3cfadc0c.pdf
Decapod
Crabs
Sudanonautes africanus
Morphology
Ondo State
Canonical Variate