Genetic relationship between five fish species of genus scarus using rapd assay

Almaaty, Ali; El_Regal, Mohamed; Mar`ie, Zeinab; Ebied, Basset

doi:10.21608/ejabf.2015.2267

Genetic relationship between five fish species of genus scarus using rapd assay

Document Type : Original Article

Authors

¹ Zoology Department, Faculty of Science, Port Said University

² Marine Science Department, Faculty of Science, Port Said University, Egypt

³ Cytogenetic Laboratory-Zoology Department- Faculty of Science (Qena) -

10.21608/ejabf.2015.2267

Abstract

Scaridae fish species including Scarus psittacus, S. frenatus, S. genazonatus, S. fuscopurpureus and S. niger, were collected from Red Sea in Egypt. The DNA fingerprints of these species were identified using RAPD-PCR technique with eight decamer primers. A total of 93 bands were scored in all species using the selected primers, with the size range varying from 190 to 2500 bp. On average, each primer amplified 11.63 bands per species. Each species had both monomorphic and polymorphic DNA bands. The polymorphic bands represent 34.41% in all fish species. The highest similarity index (70%) was found between Scarus psittacus and S. frenatus, while the lowest one (51%) was between Scarus psittacus and S. niger, S. genazonatus and S. fuscopurpureus and S. genazonatus and S. niger. Therefore the
phylogenetic relationships among the five studied scaridae species were clearly resolved in dendrogram, they were clustered into four clusters groups: the first one comprised, S. fuscopurpureus and S. niger, the second included S. psittacus, S.
frenatus and S. genazonatus, the third included S. genazonatus and the fourth branch
contained S. psittacus and S. frenatus. In conclusion, the obtained results exhibited
that the five Scarus species are distinctive ones. In addation, the RAPD patterns are
suitable tools to differentiate between fish species.

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