The Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401Effect of rearing temperature on larval growth and the development in different tilapia species114220010.21608/ejabf.2014.2200ENNabil Abdel-HakimDepartment of Animal Production, Faculty of Agriculture, Al-Azhar University,
CairoMohsen HusseinDepartment of Animal Production, Faculty of Agriculture, Al-Azhar University,
CairoMostafa MousaFish Reproduction Laboratory, National Institute of Oceanography and FisheriesEl-Sayed AttiaFish Reproduction Laboratory, National Institute of Oceanography and FisheriesFatema NemakyCentral Laboratory of Aquaculture Research, Abbasa, Abo-Hammad, SharkeyyaJournal Article20170404The present study deals with the effect of temperature on the development of different tilapia species (O. niloticus, red tilapia, O. aureus and hybrid tilapia (O. niloticus x O. aureus)) during larval rearing, and its subsequent effect on larval<br />growth and survival. The development of the gills and the digestive tract was investigated histologically and histochemically in the developing control and temperature-exposed larvae of different tilapia species. The exposure of different tilapia species larvae to moderate level of temperature (25-35ºC) with a mean of 30ºC greatly enhanced the development of gills and the digestive system of larvae as indicated by the quantitative and qualitative changes of the mucus composition from predominantly neutral to a mixture of neutral and acid mucosubstances, or acid<br />mucosubstances occurred during the rearing period. This may be due to the direct effect of temperature on food intake and increasing of metabolism, enhanced the immunity of larvae or indirect effect of temperature on sex differentiation andproducing high percentage of males which have rapid growth rate than females. Thus, rearing temperature directly or indirectly improved larval growth of different tilapia species, since a marked increase in both, length and weight of larvae occurred during the experimental period. In addition, temperature-exposed larvae also gave a<br />significantly higher survival rate than that of control. It could be concluded that the exposure of different tilapia species larvae to a rearing temperature of 30ºC was effective for improvement of development and growth in a safe, simple and acceptable manner, as well as in a friendly approach to the environment.https://ejabf.journals.ekb.eg/article_2200_ad4c4a195efca75cdbbfbeeb49a3bcd8.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401Feeding biology and biochemical composition of the lessepsian migrant Octopus aegina (Cephalopoda: Octopodidae)1527220210.21608/ejabf.2014.2202ENInas OsmanMarine Science Department, Faculty of Science, Suez Canal University, Ismailia, EgyptHowaida GabrMarine Science Department, Faculty of Science, Suez Canal University, Ismailia, EgyptSalah El-EtrebyMarine Science Department, Faculty of Science, Suez Canal University, Ismailia, EgyptSaad MohammedMarine Science Department, Faculty of Science, Suez Canal University, Ismailia, EgyptJournal Article20170404The natural feeding of 609 Octopus aegina was studied in relation to the environmental seasonal variations in three populations in the Suez Canal, the Gulf of Suez and the Mediterranean Sea. Samples were collected seasonally from March 2009 till May 2010. Analysis of the stomach contents revealed that O. aegina attained the highest percentages of empty stomachs during spring for the three populations. The (1/2) full stomachs was dominant in the collected samples (32.97 %). The study revealed that 34.43 %, 32.83 % and 32.10 % of the examined stomachs were empty in<br />the samples of Suez Canal, Gulf of Suez and Mediterranean, respectively. There was no definite seasonal food preference, and crustaceans were the most preferable food item followed by fishes. The biochemical composition of O. aegina revealed that females are higher than males in total protein content (72.41 % and 69.29 % for<br />immature and mature females respectively) and total lipid content (38.4 g/100 g dry weight and 33.2 g/100 g dry weight for immature and mature females respectively). Amino acid analysis showed that O. aegina contains higher percentage of non essential amino acids (NEAA) than essential amino acids (EAA). Leucine, Lysine and Arginine had the highest percentage of EEA, while Asparagine, Glutamate and Glycine had the highest percentage of NEAA. EAA in males increased from 36.64% to 37.93 % in immature and mature respectively, while in females it increased from<br />36.93 % to 41.5 % in immature and mature respectively. Fatty acid analysis showed that males O. aegina contain higher percentage content of fatty acid components (SFA and UFA) than females. The results also revealed that SFA tended to decrease by maturation in males and females. Palmitic (16:0) and Stearic (18:0) were the most<br />abundant SFA. Mature males had the highest percentage content of PUFA.https://ejabf.journals.ekb.eg/article_2202_535e0e96ac6380447c7aed17907f0cf1.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401Evaluation of prebiotic and probiotic dietary supplementation on growth performance and some blood parameters of Cyprinus carpio Frys2938220310.21608/ejabf.2014.2203ENWafaa ElerakyNutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine,
Zagazig University, 44511 Zagazig, Egypt.Yahya M.Pollution Laboratory, Fish research station, El-Kanater El. Kyayria, National
Institute of Oceanography and Fishers, Egypt.Rasha RedaEletreby S.Department of Marine Science, Faculty of Science, Suez Canal University, Egypt.Journal Article20170404The objective of this study was to evaluate the effect of prebiotics and probiotics on the growth performance, non-specific immunity and chemical composition of Cyprinus carpio. A total of 250 fry of Cyprinus carpio with an average body weight<br />and length were used in experiments 2.82 ± 0.12g and 3.12 ± 0.13cm were divided into five experimental groups fed the pelleted diets for 12week as follows: groups 1 (control group) fed diet (T1), groups 2, 3 fed on diets (T2, T3) which supplemented with 1.5 and 2.5 g kg-1 Organoferum dry prebiotic respectively. Groups 4, 5 fed on diets (T4, T5) which supplemented with 0.5 and 1 g kg-1 Biogreen E probiotic respectively. The results of this study revealed that fish in group 3 had significantlyhigher final body weight, weight gains and specific growth rate followed by fish in group 5. Length increments and survival rate in fish of group 3 and 5 were significantly higher (P < 0.05) than other groups. The highest crude protein and lipid content (P < 0.05) were found in the fish fed on diets T3 and T5. Total serum protein,<br />albumin and globulin were significantly increased in fish fed the experimental diets T5 than other groups. While, lysozyme activity were significantly increased in fish fed on the experimental diet T3. The conclusion of present study reveals that a dietary supplementation 2.5 g/kg prebiotics was improved growth performance, and nonspecific<br />immunity of Cyprinus carpio Frys.https://ejabf.journals.ekb.eg/article_2203_826047e2d31993446a9c3c4fbbae9a89.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401Qarun Lake fisheries; Fishing gears, Species composition and Catch per unit effort3949220410.21608/ejabf.2014.2204ENSabry El-SerafyFaculty of Science, Benha UniversityAlaa El-HaweetCollege of Fisheries Technology and Aquaculture, Arab Academy for
Science & Technology and Maritime TransportAzza El-GaninyFisheries Division, National Institute of Oceanography and FisheriesAlaa El-FarFisheries Division, National Institute of Oceanography and FisheriesJournal Article20170404Qarun Lake is a closed salty basin located about 90km southern Cairo Egypt,<br />cover an area of 230km2 with annual production of about 3000 tonnes. The present<br />study assessed the lake fishery for better management purpose. Data, information and<br />samples were collected through 42 field trips and 311 fishermen interviews during<br />fishing season (2010-2011). The lake was divided into three sub-areas, east, middle<br />and west. It was found that the fishing gears used in the lake were; 1-Trammel nets<br />with four types differed in their dimensions, mesh sizes and mainly targeting Mugil<br />cephalus, Tilapia zillii, Solea spp. and Liza spp. 2- Seine nets with three types that<br />were targeting M. cephalus, anchovy and shrimp. 3- Others fishing methods (Fishing<br />aggregation system, traps and hooks) targeting mainly T. zillii. For each gear and<br />methods used, the species composition (target and by-catch) and catch per unit effort<br />were estimated according to sub-area and season. It is realized that middle area was<br />more productive with higher species diversity. The present study recommends<br />prohibiting traps in spring and all seine nets except Gahzl Zardina during summer in<br />west and improving the fish catch and effort statistics for sustainable management of<br />this valuable resource.<br /> https://ejabf.journals.ekb.eg/article_2204_cd9c8df2ff44d552a1fdcbfe4d1c4822.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401Distribution and zonation of Goatfishes (Family: Mullidae) inhabiting the Northern Red Sea, South Sinai, Egypt.5161220510.21608/ejabf.2014.2205ENHanaa SarhanDepartment of Marine Science, Faculty of Science, Suez Canal University,
Ismailia, Egypt.Magdy AlwanyDepartment of Marine Science, Faculty of Science, Suez Canal University,
Ismailia, Egypt.Wafaa SallamDepartment of Marine Science, Faculty of Science, Suez Canal University,
Ismailia, Egypt.Redouan BsharyDepartment of Behavioural Ecology, Institute of Biology, University of Neuchâtel,
Switzerland.Journal Article20170404Goatfish species are among the most common species of the Red Sea reef communities; however, there are only few attempts to describe the distribution of them in the Red Sea. The main aim of the study is to shed light on some ecological features of the Goatfish inhabiting the Red Sea reef. Observations were conducted along the eastern coast of Sinai Peninsula, South Sinai, Red Sea, Egypt. Visual census transects were established with timed swims along the study sites which were divided into three sections: the northern sites, the middle sites, and the southern sites.<br />Six species of goatfishes were recorded (Parupeneus cyclostomus, P. macronima, P. indicus, P. forsskali, Mulloidichthys Vanicolensis, M. flavolineatus). P. macronima had the highest abundance, while M. flavolineatus had the lowest at all sites. In general, the number of species is not influenced by the sites (p = 0.774), while the number of individuals varied significantly among sites (p = 0.065). The zones have significant effect on the abundance of species, as the number of individuals was higher in the reef slope than in the reef flat (P = 0.060), and the number of species was also higher on the reef slope than the reef flat (p = 0.071). Sizes of goatfish individuals were bigger in the reef slope than the reef flat, but not in a significant way(p = 0.182). https://ejabf.journals.ekb.eg/article_2205_e05dfae7fc15bbde8f02c59acd1c7ee8.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401A Comparative Study on Lipid and Protein Composition of Oreochromis niloticus Muscles from Five Different Localities in Egypt6373220610.21608/ejabf.2014.2206ENSalwa El- NabarawyZoology department, Faculty of Science, Al Azhar University (Girls Branch),
Nasr City, Cairo.Journal Article20170404This study was aimed to compare the muscle lipid and protein composition of Oreochromis niloticus from five different localities in Egypt in order to determine the most nutritionally valuable samples based on the mode of feeding supplied. The fish samples were brought from the River Nile (natural feeding), Ismailia Canal (zooplankton feeding) and three fish aquacultures; Balteem (low protein diet, 18%),Al Abbasa (high protein diet, 30%) and Al Qanater Al Khaireya (zooplankton feeding in concrete tanks). Muscle total protein and total lipid contents were estimated. Fatty<br />acid and amino acid analysis were carried out. Studying muscle protein fractions was undertaken using SDS-PAGE electrophoresis. Results showed that the highest total proteins content was detected in the River Nile sample, while the lowest was estimated in Al Qanater fish sample. The percentage value of essential amino acids in the fish muscles from Al Abbasa was higher (45.6%) than the other four localities. Total lipids were found to have the highest value in Al Abbasa aquaculture sample whereas the lowest level was detected in the River Nile sample. Analysis of fatty acids and amino acids revealed variable differences between all the examined samples from the five localities.<br />Polyunsaturated fatty acid (PUFA) analysis showed that omega-3 had the highest % value in O. niloticus from the River Nile and Al Abbasa localities (30.30 and 30.36 respectively), while omega-6 fatty acids were higher in the fish samples from Balteem and Al Qanater (35.5% and 36.08% respectively) than the other fish samples. Elecrtophoretic data analysis showed that the highest number and percentage values of protein fractions among the five localities were recorded in sample obtained from the River Nile. It could be concluded that the best nutritional fish muscle quality was related to natural feeding in the River Nile sample and that fed on high protein diet at Al Abbasa aquaculture.https://ejabf.journals.ekb.eg/article_2206_6b0d97b4959fca45798481afe4966570.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401Reproductive biology of the squid Loligo forbesi (Cephalopoda: Loliginidae) in the Egyptian Mediterranean waters7587220710.21608/ejabf.2014.2207ENRafik RiadNational Institute of Oceanography and Fisheries, Alexandria, EgyptHamida WerfalyZoology Department, Faculty of Science, Ain Shams University, Cairo, EgyptJournal Article20170404Reproductive biology of males and females Loligo forbesi were investigated from samples obtained from commercial catch caught by local fishermen using trawling nets. The samples were seasonally collected (548 individuals) between spring 2010 and winter 2011. The results of sex ratio indicated that females outnumbered males during peak periods of<br />reproduction. Based upon morphological features of the gonads, two and five maturity stages for males and females were described, respectively. Depending on the proportion of each maturity stage, as well as various maturity indices (gonado-somatic index, G.S.I and nidamental- somatic index, N.S.I) the spawning season was found to take place during spring<br />and early summer. The mean size at first maturity was 13 and 11.8 cm. dorsal mantle length (ML) for males and females respectively.https://ejabf.journals.ekb.eg/article_2207_18d81a8f6624617eb3f1cd32d0558b67.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401Molecular characterization and organs expression of cytochrome P450 1B1 from Japanese eel (Anguilla japonica)103113220910.21608/ejabf.2014.2209ENMohamed El-kady1National Institute of Oceanography and Fisheries, Alexandria, EgyptYoshio Kaminishi1National Institute of Oceanography and Fisheries, Alexandria, EgyptTakao Itakura1National Institute of Oceanography and Fisheries, Alexandria, EgyptJournal Article20170404The CYP1 family, one of the gene families of the CYP superfamily, has four subfamilies deposited in the GenBank/EMBL so far; CYP1A, CYP1B, CYP1C, and the newly identified CYP1D. The metabolic activation and elimination of polyaromatic<br />hydrocarbons, polychlorinated biphenyls, and aryl amines from fish body is largely mediated by the CYP enzymes. A new cDNA of the CYP1B subfamily encoding CYP1B1 was isolated from Japanese eel liver after a single intraperitoneal injection of β-naphthoflavone (BNF). The full-length cDNA obtained was 2985 bp and contained a 5' noncoding region of 294 bp, an open reading frame of 1626 bp coding for 541 amino acids and a stop codon and a 3' noncoding region of 1065 bp. The predicted molecular weight of the protein was approximately 61.27 kDa. The deduced amino<br />acid sequence of Japanese eel CYP1B1 showed 62% similarity to three-spined stickleback CYP1B1 and zebrafish CYP1B1. It exhibited similarities of 66% with that of killifish, Indian medaka and our previously reported carp CYP1B1 and -1B2 while the higher similarities (67 and 69%) of the deduced amino acids was observed with that of Nile tilapia CYP1B1 and rainbow trout respectively. The percent identities of Japanese eel CYP1B1 cDNA showed similarities with those of the reported CYP1Bs of mammals of 57, 57, and 56% for human, rat, and mouse CYP1B1, respectively.<br />Japanese eel CYP1B1 was aligned with the CYP1 sequences and has been deposited in the GenBank/EMBL data bank with the accession number AY518340. The phylogenetic tree constructed using the previously reported CYP1B sequences of mammals and fish suggested the closer relationship of the newly identified Japanese eel CYP1B1 to rainbow trout CYP1B1. QRT-PCR analysis of liver, kidney, gills and intestine revealed a distinct induced expression in liver, kidney and gills (71.93, 3.87 and 539.56 respectively) while the constitutive expression (0.062) was observed in<br />intestine.https://ejabf.journals.ekb.eg/article_2209_7573bcd08589f817d33846f24278d0ca.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401Molecular Cloning and Expression of Cytochrome P450 1C1 in Japanese Eel (Anguilla japonica)115124221110.21608/ejabf.2014.2211ENJournal Article20170404Cytochrome P450 (CYP) enzymes constitute a multigene family of many endogenous and xenobiotic substances. The CYP1 family is of particular interest in environmental toxicology because its members are dominant in the metabolism of<br />polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and aryl amines. A new cDNA of the CYP1C subfamily encoding CYP1C1 was isolated from Japanese eel (Anguilla japonica) liver after intraperitoneal injection with β-<br />naphthoflavone (BNF). The full-length cDNA obtained (3508 bp) contained a 5' noncoding region of 355 bp, an open reading frame of 1581 bp coding for 526 amino acids, a stop codon, and a 3' noncoding region of 1572 bp. The predicted molecular weight of the protein was approximately 59.33 kDa. The deduced amino acid sequence of Japanese eel CYP1C1 had the lower similarity of 70% with that of killifish CYP1C1 while the higher similarity (79 and 81%) was observed with that of rainbow trout CYP1C2 and -1C1 sequences respectively. It exhibited similarities of 71% with that of Indian medaka CYP1C1 and zebrafish CYP1C2. Also the similarity of 74% was registered with the sequence of three-spined stickleback fish CYP1C1, - 1C2 and carp CYP1C2. It showed similarity of 77% with that of Nile tilapia CYP1C1,<br />scup CYP1C1 and scup CYP1C2.The phylogenetic tree showed the newly identified Japanese eel CYP1C1<br />sequence to be clustered with rainbow trout CYP1C1 and -1C2. Japanese eel CYP1C1 was aligned with the CYP1 sequences and has been deposited in the Gen Bank / EMBL data bank with the accession number AY444748. Quantitative real-time<br />polymerase chain reaction (QRT-PCR) analysis of liver, kidney, intestine and gills revealed a distinct induced expression in all organs studied (283.33, 579.35, 20.96 and 3642.32 respectively).https://ejabf.journals.ekb.eg/article_2211_ab203a1cfb5b2952760fa0ecccb86f2f.pdfThe Egyptian Society for the Development of Fisheries and Human Health (ESDFHH)Egyptian Journal of Aquatic Biology and Fisheries1110-613118220140401RAPD –PCR analysis of four coral reef fish species, genus cephalopholis (Family: serranidae) in the Red sea.125141221210.21608/ejabf.2014.2212ENAbied A.M.Zoology Dept. Faculty of Science, Qena, South Valley University, EgyptKhalil .HZoology Dept. Faculty of Science, Qena, South Valley University, EgyptAbu El Regal A.Marine Science Department, Faculty of Science, Port Said University, Egypt.Journal Article20170404The genetic relationships between four species of groupers: Cephalopholis oligosticta, C. sexmaculata, C. hemistiktos and C. argus, common coral reef fish in the Red Sea of family Serranidae were studied by using Random Amplified<br />Polymorphic DNA (RAPD) technique. Twenty random primers were used. Each primer was individually tested with the four fish species. Amplification products were resolved on agarose gel electrophoresis and visualized under UV light, then<br />photographed. The number and size of amplified DNA fragments showed a wide range of variation. Tabulation and analysis of the data using the genetic similarity coefficient were carried out. The highest genetic similarity observed was between C. hemistiktos and C argus and the lowest was between C. oligosticta and C.sexmaculata. Dendrogram analysis revealed three clusters: first, comprises C.hemistiktos and C. argus; second, contains C. oligosticta and (C. hemistiktos and C.argus); the third incompasses C. sexmaculata and (C. oligosticta, C. hemistiktos and<br />C. argus).https://ejabf.journals.ekb.eg/article_2212_3f6e6872fcb65900126df1f1690c8c70.pdf