ORIGINAL_ARTICLE
Distribution and assessment of heavy metals in the coastal area of the Red Sea, Egypt.
In order to evaluate the contamination state of the Red Sea coastal area, concentrations of heavy metals (Cu, Zn, Cd, Pb, Fe and Mn) were determined in sea water and surface sediments along the western side from Suez to Shalateen. The studied locations were selected to cover potential natural and anthropogenic sources of metal input in the Egyptian Red Sea. Levels of Cu, Zn, Cd, Pb, Fe and Mn in water were 0.85 - 2.61, 2.13 - 14.42, 1.24 - 4.51, 0.11 - 0.31, 8.44 - 33.71 and 0.30 - 0.62 µg/l; while in sediments were 25.67 - 100.77, 52.60 - 143.00, 21.20 - 63.43, 1.23 - 6.63, 11632 – 28321 and 291.43 - 803.80 µg/g, respectively. There were obvious positive correlations between Cu, Zn, Pb and Fe in water; while sediments showed positive correlations between Cd, Zn and Pb as well as between Fe and Mn. The obtained data were further subjected to Principal Component Analysis (PCA). The results lend support to the argument that specific geographical distribution of heavy metals in Red Sea could be related to different types of metal input. In Suez area, highest metal load in water and sediments was due to combined effects of wastes discharge and maritime activities. High contamination of Cd, Zn and Pb in sediments was associated with the shipping process of phosphate ore, while elevated Fe and Mn levels were principally associated with natural input of terrigenous sediments. The sediments indices of enrichment factor and geo-accumulation index showed high enrichment of Pb and Cd due to contamination from phosphate shipping; and the inflow with terrigenous sediments led to moderated to highly contamination with Fe and Mn.
https://ejabf.journals.ekb.eg/article_29246_1fece6e7318b45808ba669d284945203.pdf
2019-03-25
1
13
10.21608/ejabf.2019.29246
Heavy metals
Red Sea
Monitoring
Seawater
sediments
M. E. A.
El-Metwally
1
AUTHOR
A. I.
Othman
2
AUTHOR
Kh. M.
El-Moselhy
3
AUTHOR
ORIGINAL_ARTICLE
Descriptive Study of Some Epiphytic Algae Growing on Hydrilla verticillata (L.f ) Royle (Hydrocharitaceae) found in the Shallow Freshwater Lake Laguna de Bay (Philippines)
Most of the phycological studies in the Philippines mainly focused on seasonal variations of phytoplankton in different lentic and lotic systems, with a little concern on epiphytic algal and cyanobacterial communities associated to aquatic macrophytes. In this study, the species composition and distribution of epiphytic algae living on the submerged plant tissues of aquatic macrophyte Hydrilla verticillata (L.f) Royle found at the largest freshwater shallow lake “Laguna de Bay” in the Philippines was studied, and documented with a detailed description for each species. A total of 15 algal species belonging to 12 genera of three algal phyla were observed including, 7 Cyanophyceae, 3 Bacillariophyceae 3 Trebouxiophyceae, and 2 Chlorophyceae. Of these taxa, the occurrence of a rare microalga Franceia amphitricha (Lagerheim) Hegewald is reported for the first time in the Philippines. These taxonomic records will add to the pool of data useful for understanding the epiphytic algal flora composition of aquatic macrophytes found in Philippine freshwater bodies.
https://ejabf.journals.ekb.eg/article_29300_88c7342dfde5dcba7b0e8c80fb81d160.pdf
2019-04-01
15
28
10.21608/ejabf.2019.29300
Cyanobacteria
Hydrilla verticillata
Epiphyte
macrophytes
microalgae
Laguna de Bay
Eldrin
DLR. Arguelles
1
AUTHOR
ORIGINAL_ARTICLE
Prevalence of human polyomavirus and papillomavirus in wastewater and in stool of Egyptian patients
Recently oncogenic viruses that are greatly associated with human cancers were detected in urban sewage and other water environments worldwide. The direct contact with contaminated water and sewage can result in serious infections associated with a wide range of diseases. Human Papillomaviruses (HPVs) and Human Polyomaviruses (HPyVs) were the most common virusesthat have been detected in urban sewage worldwide. In the present study, Ninety sewage samples were collected from Zenin wastewater treatment plant in Giza Governorate. Sixty stool samples were obtained from cancer patients. The findings showed that the prevalence of HPVs in sewage and stool samples was 24.4 % (22/90) and 28.3 % (17/60), respectively. HPV16 genotype was the most predominant genotype using sequencing. On the other hand, the prevalence of HPyV in sewage and stool samples was 78.9% (71/90) and 58.3% (35/60) respectively. JC HPyV and BK HPyV were the most common genotypes of human polyomaviruses in sewage 57.8% (41/71), 42.3% (30/71), respectively and in stool 54.3% (19/35), 45.7% (16/35), respectively. By using quantitative Real-time PCR, the number of HPyV DNA copies ranged from 5.3x104 to 6.02x104 GC\L in raw wastewater and 6.2x103 to 6.85x103 GC\L in treated effluent of wastewater treatment plant. Regarding to stool samples, their numbers range were 2.5x105 and 1.24x107 GC\L. These results are indicator of risk for the prevalence of HPVs and HPyVs in Egyptian environment. This is the first report in Egypt to study the human oncogenic viruses in the same study of environmental and clinical samples.
https://ejabf.journals.ekb.eg/article_29237_6b60263f03501cb1af21fd6b7680853f.pdf
2019-04-01
29
41
10.21608/ejabf.2019.29237
real time PCR
Papillomaviruses
Polyomaviruses
Sewage
stool samples
WWTP
Nehal
I. Ahmed
1
AUTHOR
Elmahdy
M. Elmahdy
2
AUTHOR
Abdo
K. Allayh
3
AUTHOR
El-Chaimaa
B. Mohamed
4
AUTHOR
Samah
A. Loutfy
5
AUTHOR
Ahmed
Barakat
nehal_nehal20072002@yahoo.com
6
AUTHOR
Mohamed
A. Ali
7
AUTHOR
ORIGINAL_ARTICLE
Some Biological Aspect and Gonads Histology of Sepia savignyi (Blainville, 1827), in the Gulf of Suez, Egypt
This work is the first histological description of gonad maturation of Sepia savignyi and the beginning of future research on its basic biology. A total of 452 samples, ranged in dorsal mantle length from 3.9 to 29 cm with average 16.35 + 1.50 and total weight ranged from 19 to 1826.8 gm, with average 606.79 + 110.7, of which 269, female and 183 male were examined between September 2014 to May 2015 obtained from the Gulf of Suez, Egypt. The size at first sexual maturity (DML50) was 11.9 and 8.4 cm for female and male, respectively. The Gonadosomatic indices show that the females and males have two peaks in the year-round spawning period the first is in December (4.1 %) and (2.4%) and the second is in April (3.5%) and (2.2%) for female and male, respectively. Five stages of gonad maturation were established for the female and male, namely immature (I), maturing(II), mature (III), spawning (IV) and spent (V) based on macroscopic examination. Histological examination showed that each ovary and testis had various stages of oocyte and spermatocytes. Oogenesis of oocyte demonstrated seven stages of maturity in females as oogonia, early primary oocyte, late primary oocyte, previtellogenic oocyte, vitellogenic Oocyte, advanced vitellogenic oocyte and ripe oocytes. Spermatogenesis of testes divided into spermatogonia, primary and secondary spermatocytes, spermatid and spermatozoa.
https://ejabf.journals.ekb.eg/article_32325_860d36913274bae6efe28726ea9772b8.pdf
2019-05-20
43
54
10.21608/ejabf.2019.32325
Sepia savignyi
Oogenesis
spermatogenesis
gonadosomatic index
Gulf of Suez
Amal
M. Amin
1
AUTHOR
Alaa
M. M. Elhalfawy
2
AUTHOR
Hafz
M. M. Khouraiba
3
AUTHOR
Mervat
A. M. Ali
4
AUTHOR
ORIGINAL_ARTICLE
An economic study on the axes and constraints for Lake Manzala development
Lake Manzala undergoes a decline in its fish production in recent two decades. The present work aims to assess the axes and constraints for development of its fisheries. Descriptive and quantitative analyses were conducted for its production during the period from 1995 to 2016. The results revealed that Manzala Lake produced 59.6 thousand MT (14.6% total fish production in Egypt) during 1995 and declined to 42.3 thousand MT (3.4%) during 2016. Statistically, data revealed that estimated model at 10% change in tilapia production led to 78% in the lake total production, 10% change in catfish led to change in 17% of the lake total production and about 93% of annual production leads to changes in fish species. The annual increment in productivity per boat was estimated as 1.14 MT, with estimated 6% rate of change during period from 1995 to 2016 based on the linear regression of average productivity per boat. In addition, productivity per fisherman indicated that there was a progress trend estimated at 7.5 MT per year, with a rate of change estimated at 4% during the study period. And, the average productivity was slightly increased from 313.7 to 384.6 kg between 1995 and 2016, respectively. In conclusion, this study suggests some solutions for constraints which are negative affecting the lake development.
https://ejabf.journals.ekb.eg/article_32053_eff5bca290f356921cc6ecc6029a3ba6.pdf
2019-05-20
55
64
10.21608/ejabf.2019.32053
Economic study
Fish production
Lake Manzala
Constraints
Axes
Saber
M. Mostafa
1
AUTHOR
Mahmoud
Kh. Ahmed
2
AUTHOR
Alaa
M. El-Far
3
AUTHOR
ORIGINAL_ARTICLE
Genotyping and interaction-reality of Acanthamoeba, enteric adenovirus and rotavirus in drinking water, Egypt.
Access to safe water is essential to life and public health of human. Nearly, 884 million people about one-sixth of the world’s populations are consuming contaminated water. In this study, the occurrence and the molecular characterization of enteric viruses particularly; enteric adenovirus, rotavirus and free-living protozoan Acanthamoeba spp. were determined in 180 tap water samples collected from five Egyptian governorates during December 2016 to November 2017. Moreover, we aimed to verify whether these isolated Acanthamoeba spp. could play a role in transmission of enteric adenovirusand rotavirus. Multiplex semi-nested RT-PCR, PCR and sequencing were used to detect and genotype rotavirus, enteric adenovirus and Acanthamoeba. Based on the sequence analysis of the Diagnostic Fragment 3 (DF3) of small subunit rRNA gene, Acanthamoeba isolates were genotyped. The present study showed that 26.7% (48/180) of tap water samples were positive for Acanthamoeba spp. The highest occurrence rate of Acanthamoeba (36.1%) was detected in tap water samples collected from Faiyum governorate. On the other hand, viral genomes were detected in 44 samples. Rotavirusand enteric adenoviruswere observed in 15.6% (28/180) and 8.9% (16/180) of samples, respectively. Qalyubia and Faiyum governorates showed high level of viral contamination up to 50% (18/36) and 47.2% (17/36), respectively. Winter was the peak season for viral and Acanthamoeba contamination in tap water. Mixed contamination between at least one virus and Acanthamoeba was observed. Acanthamoeba lenticulata, Acanthamoeba T4, rotavirusG1P [8], and human enteric adenovirus41 were the most predominant types identified during this work. The prevalence of pathogenic Acanthamoeba genotypes, especially genotype T4 and enteric viruses (rotavirusand adenovirus) in the examined tap water samples presented health hazards to consumers.
https://ejabf.journals.ekb.eg/article_29299_1ffb968adce202cb064831a4a0213f4b.pdf
2019-04-01
65
79
10.21608/ejabf.2019.29299
Acanthamoeba
Rotavirus
Adenovirus
Genotyping
interaction
Tap water
Mahmoud
A. Gad
1
AUTHOR
Abdou
K. Allayeh
2
AUTHOR
Elmahdy
M. Elmahdy
3
AUTHOR
Mohamed
N.F. Shaheen
4
AUTHOR
Neveen
M. Rizk
5
AUTHOR
Ahmad
Z. Al-Herrawy
6
AUTHOR
Fatma El-zahraa
R. Saleh
7
AUTHOR
Mohamed
A. Marouf
8
AUTHOR
ORIGINAL_ARTICLE
Nutritional status, antimicrobial and anti-biofilm activity of Potamogeton nodosus Poir
Macrophytes have been approved as a natural resource for nutritional components as well as antibacterial and anti-biofilms agents. In this context the nutritive status and the antimicrobial activity of the Egyptian macrophytes species Potamogeton nodosus have been studied. P. nodosus have approached a high nutritional value which was reflected in lower ash (11.93%) and considerably high organic nutrient level (88.07%). In addition, this plant contains high protein, carbohydrates, potassium and nitrogen contents with very low concentrations of micro-elements (Iron, Manganese, Zinc and Copper) which make it a valuable source for fishes and animals diets. The extracts also revealed a high phenolic and flavonoids contents (19.313 mg/g gallic acid equivalent and 17.885 mg/g rutin respectively). Both antibacterial, and anti-biofilm activity of three solvents, chloroform, methanol and petroleum ether extracts of P. nodosus Poir were tested against Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 25922.The methanol extract showed the highest antimicrobial and anti-biofilm activity. The findings of this investigation suggested the potential of Potamogeton nodosus as a new supplementary food resource. In addition to the antimicrobial activity of its extracts especially methanol extract, which would have promising applications in this field.
https://ejabf.journals.ekb.eg/article_29301_4cef5967f7f2fa6b873244c61d5822b8.pdf
2019-04-01
81
93
10.21608/ejabf.2019.29301
Potamogeton nodosus
phenolic content
Flavonoids
Antimicrobial
Anti-biofilm
HPLC analysis
Amany
M. Haroon
1
AUTHOR
Said
M. Daboor
2
AUTHOR
ORIGINAL_ARTICLE
Study on the biology of the small scaled terapon Terapon puta (Cuvier, 1829) from Bardawil Lagoon, North Sinai, Egypt
This work aims to investigate some biological aspects as length-weight, age composition, sex ratio, Gonado-Somatic Index (GSI) and fecundity of the small- scaled Terapon puta (Cuvier, 1829) in Bardawil Lagoon, North Sinai, Egypt. The study was carried out during the period from May 2017 to January 2018. The results revealed that the total body weight relative to the total body length is positive isometric, b = 3.0209. The length at first capture was 11.9 cm, and the length groups 12-12.9 cm have predominated the fish population (17.8%). Four age groups were detected, and the age composition of T. puta indicated that individuals belonging to age group I dominate the fish population (38.6% of the total collected individuals). The overall sex ratio indicated that the females T. puta were dominant throughout all the months except in November. The lowest values of GSI of T. puta were recorded in November and December while its highest values recorded in June. The length at first mature (L50) was determined as 13.2 and 13.7 cm for males and females respectively. The fecundity increase with increasing the total body length with correlation coefficient (r2 = 0.89). To facilitate the management of modern fisheries of this species , the recommendation should be directed to equal length at first capture by the length at first mature and not less than 13.8 cm to give the females to spawn even once to keep stock of the fish in Bardawil Lagoon.
https://ejabf.journals.ekb.eg/article_29181_86fb110e9fa35d29a7d779c5f4e07dba.pdf
2019-03-27
95
107
10.21608/ejabf.2019.29181
Terapon puta Bardawil Lagoon Length
weight Sex ratio Fecundity
Attia
A. O. El-Aiatt
1
AUTHOR
Kariman
A. Sh. Shalloof
2
AUTHOR
ORIGINAL_ARTICLE
Remediation of toxic effect of Mercuric Chloride on the Nile Tilapia, Oreochromis niloticus using Selenium
The present results showed that heavy metals (HgCl2) have affected the biological parameters of Oreochromis niloticus. These effects depended on the duration of the exposure and the concentration of the heavy metal used. The effect of various doses level of HgCl2 on the erythrocyte count (Red blood cells) decreased under the effect of the high doses of HgCl2 (0.08 mg/l or more). A significance difference was recorded in comparison with the control fish (p<0.05).The total white blood cells counts significantly increased as the concentration of HgCl2 increase when compared to the control fish. In contrast, hemoglobin (Hb) levels significantly (p<0.05) decreased after the fish exposed to high level doses of HgCl2. Addition of selenium (Na2SeO3) at doses of 1.0 and 2.0 mg/kg to fish food for a period of 20 days of treatment with HgCl2 improved blood constituent. Treated gills with high doses of HgCl2 showed alteration on both of primary and secondary lamellae and an increase in the mucous secretion when compared with the control tissue. No abnormalities occurred in the liver of control fish. The main alterations in the liver of fish were observed after treatment with high doses level of HgCl2 (0.06-0.08mg/l). The hepatocytes were hypertrophied and the nuclei showed necrosis.
https://ejabf.journals.ekb.eg/article_33802_da8eb452d23998beb52784c6bf4d922b.pdf
2019-05-01
109
121
10.21608/ejabf.2019.33802
Remediation
selenium
mercury
Haematology
histopathology
Oreochromis niloticus
Fatma
A. El-Matary
1
AUTHOR
Mokhtar
S. Beheary
2
AUTHOR
Rania
O. Barakat
3
AUTHOR
Abd El-Hakim
A. Elgamal
4
AUTHOR
ORIGINAL_ARTICLE
Effect of some extraction techniques on properties and economic of chitosan obtained from shrimp shells waste
The present study was planned to investigate the effect of three techniques; traditional, microwave and autoclave on properties and economic evaluation for chitosan obtained from shrimp shell waste. Results showed that sun dried Suez-shrimp wastes composed (wet weight) 5.28% moisture, 7.09% (total nitrogen), 7.77% lipid and 36.15ash content. Microwave technique improved the water binding capacity (WBC) property and antioxidant propertieswhereas traditional technique improved the oil binding capacity (OBC) property. Also, there are variation in degree of deacetylation (DDA) values; 95.5% for autoclaved, 93.0 for microwaved and 88.5 for traditional chitosan samples. Economically, the traditional method is the best economic methods. Also, it provides about 45.7 thousand pounds/ton and it reduces the chances of imported chitosan and modifies of the Egyptian trade balance. In conclusion, this study recommends that shrimp shells waste should be utilized to produce chitosan as an economic and value added product and to encourage local production and reduce imported chitosan.
https://ejabf.journals.ekb.eg/article_32447_66721301f08a0bb876ea8d36de01df79.pdf
2019-05-20
123
131
10.21608/ejabf.2019.32447
Chitosan
Extraction techniques
antioxidant
Shrimp wastes
Economic Evaluation
Ibrahim,
M. A.
1
AUTHOR
Mostafa,
S. M.
2
AUTHOR
Ibrahim,
S. M.
3
AUTHOR
ORIGINAL_ARTICLE
Influences of diets supplemented with pomegranate peel on haematology, blood biochemistry and immune status in monosex Nile tilapia, Oreochromis niloticus
A total of 360 monosex Oreochromis niloticus with an average body weight of 7.1±1 g were used. The fish were randomly divided into 8 equal triplicate groups (15 fish/replicate). A basal control diet was formulated to fulfil the nutrient requirements of the fish that contained 25% crude protein (CP) and 448.3 kcal/100 g. The other 7 diets (treatment diets) were supplemented with pomegranate peel (PP) at rates of 1, 2, 3, 5, 10, 15 and 20%. The fish were fed the diets 3 times daily at a rate of 5 to 7% of the body weight per day, 6 days a week for 90 days. Haematological analysis revealed that red blood cell (RBC) counts and haemoglobin levels (Hb) were significantly lower and that white blood cell (WBC) counts were significantly (P<0.01) higher in blood from fish fed the different PP-supplemented diets than in blood from fish fed the control diet. Glucose and total protein levels were increased after 90 days. Cholesterol levels were reduced by PP, while triglyceridelevels were significantly increased. In addition, alanine aminotra-nsferase (ALT) and aspartate aminotransferase (AST) levels were significantly reduced in the blood of monosex Nile tilapia fed diets with different concentrations of PP. Immunological parameters (IgM levels and lysozyme activity) were significantly improved by PP supplementation. These results indicate that addition of PP to monosex O. niloticus diets improved immune status and liver and kidney function without any adverse effects on health status.
https://ejabf.journals.ekb.eg/article_33559_e9c3f6420f81f0ee3c03732f26af04e9.pdf
2019-04-01
133
144
10.21608/ejabf.2019.33559
Oreochromis niloticus
Pomegranate peel
hematology
biochemistry
immunity
Ahmed
Badrey
gmal_ahmed77@yahoo.com
1
Department of Zoology, Faculty of Science, Al Azhar Universtey, Assiut.
AUTHOR
Alaa
Osman
agosman2@yahoo.com
2
Department of Zoology, Faculty of Science, Al-Azhar University (Assiut branch), Assiut, Egypt
AUTHOR
Mahmoud
S. Farrag
3
Department of Zoology, Faculty of Science, Al-Azhar University (Assiut branch), Assiut, Egypt
AUTHOR
Mohamed
M. M. Toutou
4
National Institute of Oceanography & Fisheries, Alexandria, Egypt
AUTHOR
Mohsen
A. Moustafa
5
Department of Zoology, Faculty of Science, Al-Azhar University (Assiut branch), Assiut, Egypt
AUTHOR
ORIGINAL_ARTICLE
Nutrient Salts and Eutrophication Assessment in Northern Delta Lakes: Case study Burullus Lake, Egypt
This study was designed to assess the levels of nutrient salts and eutrophication status in Burullus Lake water. Most of the physicochemical characteristics showed wide variations among the sites and seasons (p<0.01). The water of the lake was in the alkaline side, and pH value fluctuated in the range (7.47-8.96). The lake water was oxygenated around the year (2.10 and 13.40 mg/l). The nutrient salts showed a lot of fluctuations, often occurring in high concentrations, with a remarkable spatial and temporal difference (p<0.001). As a result of nutrient enrichment, Chlorophyll-a (Chl-a) recorded abnormal high concentrations up to 648.3 µg/L. TN:TP ratio implied that phosphorus is the limiting factor for phytoplankton growth in Burullus Lake. The high levels of nutrient salts and Chl-a together serve as a good indicator of hypertrophic levels in the lake water. This observation was confirmed by the data of the Trophic State Index (TSI) using Chl-a, TP, TN, and Secchi depth that indicated the hypereutrophic state of Burullus Lake associated with poorly of its water quality. Our results indicated the existence of dramatic deterioration in Burullus Lake because of human activities and the discharge of wastewater to the lake.
https://ejabf.journals.ekb.eg/article_30239_60335922684e8c5e9b480c2fac31a6d4.pdf
2019-04-16
145
163
10.21608/ejabf.2019.30239
Burullus Lake
Eutrophication
N:P ratio
total nitrogen
Total phosphorus
Trophic State Index
Fathy
A. Elsayed
1
AUTHOR
Mohamed
A. Okbah
2
AUTHOR
Seliem
M. El-Syed
3
AUTHOR
Manal
A. Eissa
4
AUTHOR
Mohamed
E. Goher
5
AUTHOR
ORIGINAL_ARTICLE
Monitoring of Oxidative Stress Biomarkers and Toxicity of Lead and Mercury in Catfish of Lake Mariout, Egypt: The Role of Meso -2, 3-Dimercaptosuccinic Acid (DMSA)
The present investigation was designed to evaluate the dangerous effects of the Pb and Hg on Clarias gariepinus inhabiting Lake Mariout and assay the possible protective effect of meso-2, 3 dimercaptosuccinic acid (DMSA). The test fish were divided into four groups kept in plastic tanks (20 fish each) containing 30L of Lake Mariout water. Group (1): Standard group (zero time); Group (2): Unexposed DMSA group, Group (3): Low dose DMSA group; Group (4): High dose DMSA group. Fish exposed to DMSA showed significant decrease in lead (P≤0.05) and mercury concentrations (P≤0.05) in kidney, liver, gills, muscle and blood than unexposed group. The present results clearly indicate a significant decrease in RBCs, Hb, Hct, and platelet counts while a significant increase in MCH, MCHC, and WBCs in the Clarias gariepinus collected from the main basin of Lake Mariout. A marked significant decrease in AST, ALT, urea, creatinine (P≤0.05) was observed in DMSA groups. A significant increase in CAT, GPX and SOD was observed after exposure to DMSA. In addition, DMSA exposure improved the histopathological alterations in fish liver and kidney.
https://ejabf.journals.ekb.eg/article_30238_10ef8a695b4898ef68c710bdc871ebc1.pdf
2019-04-16
165
182
10.21608/ejabf.2019.30238
Monitoring
Oxidative Stress
Lead
mercury
toxicity
catfish
Lake Mariout
Nema
A. Mohamed
1
AUTHOR
Awatef
M. Ali
2
AUTHOR
Shnoudy
A. Bakhoum
3
AUTHOR
Heba
H. Abdel-Kader
4
AUTHOR
Mohamed
A. Ahmed
5
AUTHOR
ORIGINAL_ARTICLE
Survivorship and growth rates for some transplanted coral reef building species and their potential for coral reef rehabilitation in the Red Sea
The deterioration of coral reefs in Egypt is a serious environmental problem. As part of studying the reef habitats rehabilitation; about 180 specimens of 8 hard coral reef species belonging to 2 families (Pocilliporidae and Acroporidae) were transplanted using table-type galvanized steel frameworks. Survival and growth rates were measured in situ then monitored over 24 months. From the transplanted 180 specimens, 128 fragments representing 71% were still survived after 4 months decreased to 121 fragments with a percentage of 67.2% after 12 months increased to 123 fragments (after recovery of two specimens) with 68.3% of the transplanted specimens after 24 months. Significant differences (P<0.05) in growth rates were observed among the three periods of investigation (4, 12 and 24 months) in both families. The mean growth rates of investigated species of Pocilliporidae; S. pistillata, P. damicornis and P. verrucosa after 4, 12 and 24 months were; 1.27±0.06 cm yr-1, 1.2±0.07 cm yr-1 and 1.03±0.07 cm yr-1 (F = 3.43, 3.16 and 4.95) respectively. The investigated species of Acroporidae; Acropora tenuis, A. digitifera, A. horrid, A. samoensis and A. variabilis recorded the annual mean growth rates of;0.56±0.02 cm yr-1, 0.45± 0.04 cm yr-1, 1.04± 0.05cm yr-1, 0.83± 0.04cm yr-1 and 0.98± 0.03cm yr-1 (F = 17.58, 0.59, 0.60, 1.50 and 2.83) respectively. New coral reef recruits were observed for S. pistillata, P. verrucosa, P. damicornis and A. degitefera, but the coral recovery was observed for S. pistillata only.
https://ejabf.journals.ekb.eg/article_30291_073bfba58ed293f6abf5f38002bb89cd.pdf
2019-04-01
183
193
10.21608/ejabf.2019.30291
Coral reefs
Red Sea
transplantation
Rehabilitation
Acroporidae
Pocilloporidae
Mostafa
A. M. Mahmoud
1
AUTHOR
Mahmoud
A. Dar
2
AUTHOR
Hussein
N. M. Hussein
3
AUTHOR
Mohamed
E. A. El-Metwally
4
AUTHOR
Mahmoud
M. Maaty
5
AUTHOR
Mohsen
Y. Omar
6
AUTHOR
Mahmoud
R. Seraj
7
AUTHOR
Tarek
A.A. Mohammed
8
AUTHOR
ORIGINAL_ARTICLE
Snailicidal, antimicrobial, antioxidant and anticancer activities of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus fungal extracts
Fungi are capable of producing a wide variety of secondary metabolites. Entomopathogenic fungi along with species which parasitize nematodes represent excellent candidates for bioactive compounds production. This study aimed at exploring the snailicidal, antimicrobial and antioxidant activities of ethyl acetate and acetone extracts of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus. Ethyl acetate and acetone extracts were prepared and tested against Biomphalaria alexandrina snails, their antioxidant and antimicrobial activities were alsoinvestigated. The anticancer activity of P. lilacinus acetone extract was tested on hepatocellular carcinoma (HepG2) cells. Acetone extract of P. lilacinus (LC50 =120 ppm) was more toxic to B. alexandrina snails than that of B. bassiana (LC50= 231 ppm). Sublethal concentrations of both extracts adversely affected survival rate, histological pattern of digestive and hermaphrodite glands of the tested snails, besides their genotoxicity to DNA of snails. P. lilacinus acetone extract showed the highest antioxidant activity. Also, itsantimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans was high. Moreover, itwas cytotoxic to HepG2 cells (IC50 =2.81 µg/ml). GC/MS analysis indicated the presence of 37 compounds in B. bassiana acetone extract with 2methylhept2,6dien4ol and 1,3dimethoxy2(hydroxymethyl)9hxanthene as major compounds. While 48 compounds were identified in P. lilacinus acetone extract where hexadecanoic acid, methyl ester and 10, 13 octadecadienoic acid, methyl ester were the major compounds. In conclusion, B. bassiana and P. lilacinus are excellent sources of bioactive compounds which have multiple biological effects.
https://ejabf.journals.ekb.eg/article_30550_d4f0d70f306ad503ce211285e650fd5e.pdf
2019-04-25
195
212
10.21608/ejabf.2019.30550
Entomopathogenic fungi
Paecilomyces lilacinus
B.alexandrina Antimicrobial
antioxidant
Anticancer
Marwa
T. A. Abdel-Wareth
1
AUTHOR
Mosad
A. Ghareeb
2
AUTHOR
Mohamed
S. Abdel-Aziz
3
AUTHOR
Ali
M. El-Hagrassi
4
AUTHOR
ORIGINAL_ARTICLE
Effect of biofloc system on the water quality of the white leg shrimp Litopenaeus vannamei reared in zero water exchange culture tanks
A 90-day trial was conducted to study the effects of different protein levels (25, 30 and 35% CP) and different carbon sources (sugarcane bagasse "SB" and wheat flour "WF") with biofloc and one control (45% CP without biofloc), on water quality parameters of the white leg shrimp Litopenaeus vannamei juveniles (0.23g ± 0.04) in zero-water exchange culture tanks. Six biofloc treatments and one control without BFT were managed. Temperature and salinity did not show any significant difference between the control and biofloc treatments, and they were at the optimum range for L. vannamei cultured. The PH value was unexpected in the bioflocs treatments. BFV and TSS were significantly higher (P ˂ 0.05) in biofloc treatments compared to the control. Concentrations of TAN and NO2 at biofloc treatments were lower compared to the control (P ˂ 0.05), the addition of different levels of protein and different carbon sources into the zero water exchange system for shrimp culture can effectively increase the activities of nitrogen cycle bacteria, which can thus reduce inorganic nitrogen levels and gradual increases of both BFV and TSS levels.
https://ejabf.journals.ekb.eg/article_33952_9eefa5cce20385e07612de08429f39cd.pdf
2019-04-01
133
144
10.21608/ejabf.2019.33952
Litopenaeus vannamei
White leg shrimp
Biofloc system
water quality
Shrimp culture
Yassien,
M. H.
1
AUTHOR
Khoreba
H. M.
2
AUTHOR
Mohamed
M. A.
3
AUTHOR
Ashry
O. A.
4
AUTHOR
ORIGINAL_ARTICLE
Evaluation of some tilapia species as biomarkers for pesticides accumulation levels at Lake Edku, Egypt.
Three tilapia species; Oreochromis niloticus, Oreochromis aureus and Tilapia zillii were evaluated as biomarkers for five different organophosphorus pesticides (Diazenon, Lindan, Parathion, Malathion and Chlorpyrifos) at Lake Edku, Egypt. O. niloticus reflected highest accumulated pesticides comparing with T. zillii, which reflected lowest accumulation levels of Diazenon, Lindan, Parathion, Malathion and Chlorpyrifos respectively. O. niloticus, O. aureus and T. zillii showed varied antioxidant activity patterns corresponding with pesticide accumulation levels. O. niloticus was superior for Superoxide dismutase activity comparing with O.aureus and T. zillii, which reflected lower level respectively. Moreover, T. zillii reflected the lowest Catalase activity comparing with O. aureus that showed the highest activity. O. niloticus showed high glutathione Peroxidase activity comparing with O. aureus and T. zillii. Glutathione reductase reflected distinguishable variation among O. niloticus, O. aureus, and T. zillii. Glutathione - S-transferase enzyme activity was decreased for T. zillii comparing with O.niloticus and O.aureus. As protein fraction, Glutathione S-transferase (GST) was expressed as one single band with different protein content. Glutathione S-transferase (GST) gene sequence of T. zillii reflected the highest genetic similarity (92.81%) with GST reference sequence. The lowest genetic similarity for GST gene sequence remarked (75.29%) O. niloticus. O. aureus showed moderate genetic similarity (87.11%) comparing with the reference gene sequence. It could be concluded that, significant correlation was detected among different organophosphorus pesticides accumulation and activity levels. Protein content and sequencing of many antioxidant enzymes indicated promising potential of employing three Tilapia species O. niloticus, O. aureus and T. zillii as biomarkers.
https://ejabf.journals.ekb.eg/article_30551_3c127d58481d9ae301d88060b06f3dfb.pdf
2019-04-25
213
226
10.21608/ejabf.2019.30551
O. niloticus
O. aureus
T. zillii
Organophosphorus pesticides
biomarker
Antioxidant activity
Glutathione S-transferase (GST)
Asmaa
A. K.
1
AUTHOR
ORIGINAL_ARTICLE
Effect of dietary Lacto cel-con probiotic on growth performance and hematology indices of fingerlings mono-sex Nile tilapia (Oreochromis niloticus)
A 120 day's feeding study was designed to study the effect of Lacto cel-con probiotic on the growth performance, hematology indices and body composition of Nile tilapia. Zea mays was substituted by sorghum in five formulated diets with inclusion levels of (0,25,50,75 and 100%). Fingerlings of mono-sex Nile tilapia with an initial weight of (1.94±0.13g) were distributed in1m3hapa fixed in earthen pond at stocking rate of 40 fish/replicate. The highest growth performance parameters in terms of (weight gain and specific growth rate) and nutrient utilization (feed conversion ratio and net protein utilization) were recorded by replacing 75 and 100% of Zea mays. On the other hand, blood indices include, Red blood Cell (RBCs) count, Lymphocyte, Hemoglobin concentration and Hematocrit value) displayed non-significant different among treatments. However, the values of White Blood Cell count (WBCs) were shown remarkable significance (P<0.05) with DS75 and DS100% diets. No significance differences were obtained in carcass composition of tilapia fed in different sorghum levels. Results showed that sorghum meal supplemented with (0.3% Lacto-cel con) can be entirely substituted up to75% of corn meal in fingerlings of mono-sex Nile tilapia diets without negative effects on growth performance, nutrient utilization, some blood parameters and body composition. It's conclude that (DS75) is a potential protein-rich with low cost feeding diet for mono-sex Nile tilapia (Oreochromis niloticus).
https://ejabf.journals.ekb.eg/article_30982_24dc752a2d476bffb0c3032d7b1037e6.pdf
2019-05-01
227
239
10.21608/ejabf.2019.30982
Sorghum level
Growth performance
feed utilization
Nile tilapia
blood parameters
Lactocel-con probiotic
Abdel-Moniem
M. Yones
1
AUTHOR
Mohsen
S. Hussein
2
AUTHOR
Mohamed
W. Ali
3
AUTHOR
Abdel-Azem
M. Abdel-Azem
4
AUTHOR
ORIGINAL_ARTICLE
Performance assessment of Re-use Mariculture System for Mini Farm in Rearing Sparus aurata and Dicentrachus labrax
The objective of the present research is to assess the performance of re-used mariculture system on growth rate and percent survival of S. aurata and D. labrax fish; more over to evaluate water quality, which affects fish production. The results revealed that the degree of re-used sea water was 96.7% which equal 65 L daily and the new water added /Kg fish was 8.8 L/Kg juvenile S. aurata daily and 3.28 L/Kg fries D. labrax daily. The physicochemical and biological water measurement (T°c, pH, S%, DO, NH3, NH4+, NO2-, NO3- and BOD) are in acceptable range in the two experiments for S. aurata and D. labrax. The result explained that the survival rate were 96% and 91% for S. aurata and 76.7% and 81.6% for D. Labrax in Tank1 and Tank2 respectively. The growth performance parameters (WG, ADG, SGR, FCR, PER and K) were reported for the two experiments. The length-weight relationship for S. aurata indicate that the high length, the increase in weight with R2 = 0.9596 and 0.9966 for Tank1 and Tank2 comparing with 0.8474 for control. Therefore the significant success of rearing S. aurata and D. labrax in RMS for mini farm was achieved in water management by improving the efficiency of biological filter.
https://ejabf.journals.ekb.eg/article_30981_df6d37d782d46e0b58e7bbc98effc41f.pdf
2019-05-01
241
252
10.21608/ejabf.2019.30981
Mariculture
Re-used water
growth rate
Minifarm
Sparus aurata
Dicentrachus labrax
Amr
M. Helal
1
AUTHOR
Mona
M. Mustafa
2
AUTHOR
ORIGINAL_ARTICLE
Possible Benefits of Using Two Dietary Crude Protein Levels And Two Fat levels From Three Sources in Feeding Tilapia
This study was conducted during the summer season (June- October) 2018 in fish farm at Al-Manzala city for 150 days. A total number of 750 mono-sex Nile tilapia with an average initial body weight of 52.6 ± 0.40 g were used in five experimental treatments (150 fish/hapa/treatment, 10 fish/m3) to evaluate two dietary crude protein levels (25 and 30%) and two crude fat levels (2 and 6%) from three fat sources (corn oil, imported fish oil, and a new local commercial source of fatty acids named Aquafat-omiga). Fish were stocked into net hapas, each hapa has the dimensions 3 x 5 x 1 m. Conclusively, from the obtained results and due to the view point of the producers, 2 % fish oil supplemented diet (25 % protein) is the most economic diet (lowest feed intake, best feed conversion and feed efficiency) followed by that (25 % protein) supplemented with 2 % Aquafat-omiga. But from the view point of the consumers, 30 % crude protein diet plus 6 % addition corn oil gave best body gain and fish carcass protein, boneless meat and lean meat besides lowest fat and ash in fish body and lowest serum cholesterol, uric acid, and cortisol. So, it could recommend using the first diet containing 30 % crude protein and supplemented with extra 6 % corn oil. Particularly, fish oil is rear, unavailable, imported, and expensive.
https://ejabf.journals.ekb.eg/article_31017_d91c9ef8661749092b9fa91d0bfdca00.pdf
2019-04-30
253
263
10.21608/ejabf.2019.31017
NileTilapia
dietary fat
fatty acids
Dietary protein
performance
Blood profile
body composition
Abdelhamid
M. Abdelhamid
1
AUTHOR
Ahmed
A. Gabr
2
AUTHOR
Fathy
F. Khalil
3
AUTHOR
Mohamed
A. A. Abdulaal
4
AUTHOR
ORIGINAL_ARTICLE
Antibacterial activity of some seaweeds from the Red Sea coast of Egypt.
Some commonly occurring brown algae Turbinaria ornata,Cystosiera myrica and Padina pavonica have been collected from the Red Sea coast of Egypt. Three different solvents namely acetone, isopropyl alcohol and ethanol were used for preparing crude algal extracts. The antibacterial activities of crude extracts were evaluated against three pathogens: Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli)by disc diffusion method, and then inhibition zones were compared. Strong antibacterial activity was observed for T. ornata,giving high potent with isopropyl alcohol against S. aureus and E. coli, with acetone against P. aeruginosa and with ethyl alcohol against S. aureus (inhibition zone >14 mm). Extracts of P. pavonica and C. myrica showed lower antibacterial activity zones, with no inhibition for most tested trials. Among solvents, isopropyl alcohol showed the highest inhibitory action, followed by acetone with moderate antimicrobial activity while ethanol revealed low antibacterial activity, giving inhibitory action only against Gram positive bacteria (S. aureus). Gram positive bacteria were more susceptible to the algal extracts than Gram negative bacteria. The present study revealed that T. ornata is a promising alga to act as an antimicrobial agent in food products, pharmacology and medical applications.
https://ejabf.journals.ekb.eg/article_31016_e070e62afb90f81cbb0ebd45cd553338.pdf
2019-05-01
265
274
10.21608/ejabf.2019.31016
seaweed
Antibacterial activity
Red Sea
Pathogenic bacteria
Phaeophyta
Fedekar
F. Madkour
1
AUTHOR
Gihan
A. El-Shoubaky
2
AUTHOR
Mohamed
A. Ebada
3
AUTHOR
ORIGINAL_ARTICLE
Molecular and Histological Studies of Salinity effect on gills and liver of Coptodon zillii in Egypt
The good growth performance of tilapia species has been recorded in nearly isosmotic salinities. The present study aimed to evaluate the effect of salinity on the structure of Coptodon zillii, being one of the most salinity tolerant tilapia species. Qaroun Lake looses water by evaporation which affects the water levels of the lake and its salinity, recently was subjected to a sharp rise in salinity degrees, which led to a sharp decrease in the rate of fish production. Histological, histochemical, immunohistochemical and genetic studies are used as accurate tests for evaluating the effects of salinity in Tilapia fish. The results showed that fish obtained from Wadi El Rayan1 showed semi typical structure of gill and liver, others from Wadi El Rayan2 showed slight deviations from normal one, on the contrary, samples from Qaroun lake exhibited a series of severe variations; histological changes as severe degeneration, necrosis, edema, hemorrhage, and Hemosidrin, as well as, a reduction in the distribution of carbohy-drates, an increase of collagen amount and a strong caspase-3 expression in gill and liver cells. Moreover, the genetic results indicated a clear sensitivity of prolactin gene to different degrees of salinities, which can possibly control the euryhalinity capacity of C. zillii. All of these results demonstrated the strong effect of the high elevation of salinity on fish production and the histological structure of the internal tissues of one of the most important fish in Egypt.
https://ejabf.journals.ekb.eg/article_31015_d7d36e52999775ed2404d1c37e0586c5.pdf
2019-05-01
275
290
10.21608/ejabf.2019.31015
Coptodon zillii
salinity
gills
Liver
Caspase-3
prolactin
Amira
I. Nofal
1
AUTHOR
Nahla
H. El-Shaer
2
AUTHOR
Amany
E. Nofal
3
AUTHOR
ORIGINAL_ARTICLE
Comparative study on antimicrobial activity of commercial and extracted chitin and chitosan from Marsupenaeus japonicus shells
Chitin was extracted from the skeleton of Marsupenaeus japonicus shrimp shell waste with a yield of 27.7% of the net weight through three steps namely; deproteinization, decolourization and demineralization. After deacetylation process of extracted chitin, chitosan was produced and represented 55.1% of the extracted chitin. Scanning electron microscope and Fourier Transform Infrared spectroscopy (FTIR) analyses were conducted to characterize the obtained chitin and chitosan. The FTIR analysis revealed different absorption bands within 4000-400 cm-1 range; by comparison these bands were found to be similar to those of commercial chitin and chitosan and confirmed partial deacetylation in the produced chitosan. Moreover, the antimicrobial activity of commercial and extracted chitin and chitosan were examined against different pathogenic Gram positive, Gram negative bacteria and fungi using agar well diffusion test. Results showed that there was no significant difference p < 0.05 in the antimicrobial activity of the extracted compounds and commercial ones against test pathogens as the extracted compounds recorded inhibition zones ranged from 12 to 23 mm in diameter while the commercial compounds recorded inhibition zones of diameters ranged from 12 to 22 mm only; with Staphylococcus aureus ATCC 25923 being the most affected microbe with inhibition zone of 23 mm produced by extracted chitin, while the lowest zone of inhibition was recorded against Enterococcus faecalis ATCC 29212 with only 12 mm by commercial and extracted chitosan.
https://ejabf.journals.ekb.eg/article_31536_14bf4a57da029c75785a7d094f6e20c1.pdf
2019-05-01
291
302
10.21608/ejabf.2019.31536
chitin
Chitosan
Marsupenaeus japonicas
FTIR
antimicrobial activity
Eman
H. Zaghloul
1
AUTHOR
Hassan
H.A. Ibrahim
2
AUTHOR
ORIGINAL_ARTICLE
Zooplankton groups in Lake Timsah, Suez Canal, Egypt
To shed some light on distribution, abundance and diversity of zooplankton groups in Lake Timsah, four cruises (spring 2015, summer 2015, autumn 2015, and winter 2016) were carried out. In the present result, Lake Timsah zooplankton were comprised 13 groups of the animal kingdom include, Copepoda (with relative abundance of 39.44%); Rotifera (32.72%); Mollusca (15.15%); Tintinnida (4.55%); Polychaeta (3.43%); Cirripedia (1.62%); Decapoda (1.24%); Cladocera (0.94%); Ostracoda (0.57%); Appendicularia (0.19%); Fish egg (0.06%); Foramenifera (0.04%) and Cnidaria (0.04%). Copepoda was found to be the most diversified group during the study period, it represented by 62 species comprised 44.93% of the total recorded species, followed by Rotifera (33 species, 23.91%), Tintinnida (18 species, 13.04%), Cladocera (10 species, 7.25%), Ostracoda (5 species, 3.62%), Mollusca (4 species, 2.90%), Appendicularia (3 species, 2.17%), Cnidaria (2 species, 1.45%) and only one species of Foramenifera represented 0.72% of the total recorded zooplankton species. Seasonally, zooplankton was flourished in summer, while winter is the lowest abundant season. Spatially, because of the difference of water masses entering the lake, each group is dominant in a certain region. Copepods are common in sites that near of Suez Canal due to the presence of saline water masses. While, rotifers and other freshwater zooplanktons are dominant in sites that near freshwater masses, such as western lagoon.
https://ejabf.journals.ekb.eg/article_31461_745361a040b39d79e5883b365568e52c.pdf
2019-05-12
303
316
10.21608/ejabf.2019.31461
Zooplankton
Distribution
Lake Timsah
Abundance
diversity
Suez Canal
Khalied
A. El-Damhougy
1
AUTHOR
Hussein
A. El-Naggar
2
AUTHOR
Mohamed
A. Aly-Eldeen
3
AUTHOR
Mohamed
H. Abdella
4
AUTHOR
ORIGINAL_ARTICLE
Mesozooplankton of the Damietta Branch, River Nile, Egypt.
Mesozooplankton abundance and distribution at Damietta Branch, Nile River was studied to assess the impacts caused by human activities. The sampling was done monthly at seven stations during October 2013-September 2014. A plankton ring net with 180 µm mesh size was used to avoid the bias toward the collection of smaller mesozooplanktons such as rotifers and copepod larvae. Thirty-six mesozooplankton taxa were recorded. Cladocera was the most abundant group (41.6%), followed by Copepods (29.9%), Rotifera (14.8%), and Ostracoda (13.2%). The decrease in rotifers density in favor of copepods and cladocerans in the ROT:CLA:COP ratio suggesting an increase in the area eutrophication. According to SIMPER analysis, the most important taxa were Bosmina longirostris, Ceriodaphnia reticulate, Moina micrura, Acanthocyclops americanus, Brachionus calyciflorus, and Candona subgibba. Multivariate analysis indicated that the variations in species distribution were associated with environmental factors, especially water temperature, conductivity, and nitrate. The degree of anthropogenic disturbance determines the quality and quantity of plankton in the study area. Consequently, the discharged post-cooling waters should be controlled to protect zooplankton fauna and in consequence the planktivorous fish of the study area.
https://ejabf.journals.ekb.eg/article_31669_e4e85628b54541db7625cc91c33a29b6.pdf
2019-05-15
317
330
10.21608/ejabf.2019.31669
Mesozooplankton
River Nile
Damietta Branch
diversity
Distribution
Eutrophication
Wael
S. El-Tohamy
1
AUTHOR
Samar
N. Abdel-Baki
2
AUTHOR
ORIGINAL_ARTICLE
Steps for rehabilitation of a Lake suffering from intensive pollution; Lake Mariut as a case study
Due to the acute water scarcity facing many urban areas, the water resource management, and lake rehabilitation become a global interest. Before applying the appropriate approach in the rehabilitation (restoration) of a hypereutrophic lake, the assessing of the point sources of pollution and their effects are A MUST. Lake Mariut is an example of lentic polymictic closed coastal Egyptian lake. The economic and recreational values of Lake Mariut were deteriorated since 1960s, as a result of using its Main Basin as a sink of wastewaters discharged from the agricultural Umum Drain and the heavily polluted Qalaa Drain mixed with primary treated sewage waters from Alexandria East Wastewater Treatment Plant. The aim of the current study is to find the suitable solution/s for the Lake Mariut Main Basin restoration, by (1) studying the general characteristics of its drainage water system (Umum and Qalaa Drains) at their downstream parts before reaching the lake at its southern side as well as the adjacent non-polluted Lake Mariut Fishery Basin. (2) Discussing (by calculation) the applicability of two proposed improvement solutions. The results reveal that, in the first the water quality of the anoxic Qalaa Drain must be improved by diluting its waters with oxic Umum Drain waters via Lake Mariut Fishery Basin prior discharging. This solution is found not sounding as it difficult to attain from practical point of view. The other alternative is by diverting the polluted Qalaa Drain point source itself away from Lake Mariut Main Basin water body which is sounding and highly recommended.
https://ejabf.journals.ekb.eg/article_31838_c3a8017fb43f5623553a440d1177cee1.pdf
2019-05-15
331
345
10.21608/ejabf.2019.31838
Lake Mariut
Qalaa Drain
Umum Drain
Rehabilitation
Pollution Prevention
Egypt
Osman
A. El-Rayis
1
AUTHOR
Enjy
I. Hemeda
2
AUTHOR
Nashwa
A. Shaaban
3
AUTHOR
ORIGINAL_ARTICLE
Comparison Between Effects of Sinking and Floating Diets on Growth Performance of the Nile Tilapia (Oreochromis niloticus)
The present study was conducted over 96 days in order to compare between the effects of floating and sinking diets with different levels of a probiotic on growth performance, food utilization and chemical composition with all-male mono-sex juvenile Nile tilapia (Oreochromis niloticus). The treatments were two floating and sinking diets supplemented with three levels of the probiotic PRO-LYNE®, being 0%, 1% and 2% of the diets. Each hapa (measuring 2 × 3 × 1 m3) was suspended in an earthen pond (4000 m2). There were 6 treatments, each consisting of 2 replicates, stocked with fish of average initial body weigh 60 g. A total of 366 fish were randomly distributed into 6 experimental groups. The stocking density was 5 fish / m3 for all the hapas. Fingerlings were fed a commercial diet containing 25.2% crude protein and at feeding rates of 4% of their fresh biomass in each hapa for the first 1.5 month and 3% until the end of the experiment. The results of the present study revealed that performance and production and economic efficiency of Nile tilapia reared in net hapas in earthen ponds significantly increased, when they were fed the floating pellets supplemented with probiotic at level 1%.
https://ejabf.journals.ekb.eg/article_31849_ccd36dfac77325254ba64f36e8437bb3.pdf
2019-05-16
347
361
10.21608/ejabf.2019.31849
Nile tilapia
Floating diets
probiotic
growth parameters
feed utilization
Chemical composition
Abdelhamid
M. Abdelhamid
1
AUTHOR
Mahmoud
F. Salem
2
AUTHOR
Mohammed
El-Sh. Ramadan
3
AUTHOR
ORIGINAL_ARTICLE
Bioaccumulation of Heavy Metals and Physiological/Histological Changes in Gonads of Catfish (Clarias gariepinus) inhabiting Lake Maryout, Alexandria, Egypt
The current study investigated the bioaccumulation of heavy metals, as well as, physiological and histological alterations, in gonads of Catfish (Clarias gariepinus) inhabiting the polluted location (main basin) and a relatively clean area (southwest basin) at Lake Maryout. A significant (P<0.05) decrease was recorded in muscle total protein and lipid in fish collected from the main basin in comparison to the southwest basin. In contrast, a significant (P<0.05) increase in muscle water content was detected in fish collected from the main basin. A significant (P<0.05) decrease in LH, FSH, estrogen, and progesterone hormones, as well as the activities of the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), were detected in fish collected from the main basin in comparison to the southwest basin. Histological observation of ovary showed lytic ovary with some stages of oocytes include early pre-vitologenic, atretic late pre-vitologenic, wide inter-follicular space and loose tunica albugenia. On the other side, testes pathologies showed completely disorganized lobule structure, accompanied by a reduced number or disappeared of germinal cells, increased interstitial space with reduced interstitial cells. In conclusion, this study confirmed that the main basin of Lake Maryout suffered from great pollution that affected completely on the fish population and the situation needs the rapid intervention of the Egyptian government to stop agricultural, industrial and health drainage in Lake Maryout by establishing treatment units before direct drainage in the Lake
https://ejabf.journals.ekb.eg/article_32036_1999b9c279778292d26f773d85c33e6d.pdf
2019-05-18
363
377
10.21608/ejabf.2019.32036
Lake Maryout
Clarias gariepinus
Heavy metals
Antioxidant enzymes
Sex steroids
histopathology
Heba
H. Abdel-Kader
1
AUTHOR
Mohamed
H. Mourad
2
AUTHOR
ORIGINAL_ARTICLE
Environmental Remediation of Tilapia Aquaculture Wastewater Using Ceratophyllum demersum and Lemna minor
Two aquatic macrophytes, Ceratophyllum demersum and Lemna minor, were used in aquaculture wastewater treatment. Tilapia fish (Oreochromis niloticus) with total body weight of 50-60 g were placed in 60 L aquaria. Four treatment groups were included: control (no plant treatment, depended on artificial filtering for purification), Lemna minor (Lm), Ceratophyllum demersum (Cd), and mixed plants group (Lm+Cd). Each group had three replicates (5 fish/replicate). The experiment was conducted for 4 weeks. Physicochemical parameters of aquaculture wastewater including pH, total dissolved solids (TDS), conductivity, turbidity, dissolved oxygen (DO), chemical oxygen demand (COD), biochemical oxygen demand (BOD), total phosphorous (TPO4), nitrate (NO3), nitrite (NO2), and ammonia were determined. The results reflected more effective removal of pollutants from fish aquaria by both plants than that by the artificial aeration/filtering. The effectiveness of pollution removal was higher in Cd group > Lm > Lm+Cd, compared to the control. This order of phytoremediation was confirmed by improvements in tilapia fish health status including liver function (AST, ALT, albumin), kidney function (creatinine, urea, uric acid), in addition to other nutritional and hematological parameters. It could be concluded that the phytoremediation using C. demersum is ecofriendly and effective in removing contaminants from Tilapia aquaculture wastewater and therefore, it is recommended to be applied in tilapia farms.
https://ejabf.journals.ekb.eg/article_31974_f952b2ced4da73cb7256173ab548fb20.pdf
2019-05-16
379
396
10.21608/ejabf.2019.31974
Oreochromis niloticus Phytoremediation
Ceratophyllum demersum
Lemna minor
Aquaculture
Wastewater
pollution
Mokhtar
Beheary
1
AUTHOR
Basma
M. Sheta
2
AUTHOR
Menna
Hussein
3
AUTHOR
Mona
Nawareg
4
AUTHOR
Fatma
A. El-Matary
5
AUTHOR
Ayman
Hyder
hyder@du.edu.eg
6
AUTHOR
ORIGINAL_ARTICLE
Ecological studies on the hermit crab Calcinus latens (Paguroidea: Diogenidae), from Aqaba Gulf, Egypt; Part (II), some morphological aspects of intersexuality phenomenon
The present study aims to shed light on some morphological aspects of intersexuality along with sexual dimorphism in the population of Calcinus latens (Randall, 1840). It is based on the same sample and so, the same measurement data of part I of some ecological studies that were performed on C. latens. So, the examined specimens were 442 (121 males, 46 intersex individuals, 275 females) collected from the Lagona region in Dahab city, Aqaba gulf, Egypt. The measurements included shield length (Sl), shield width (SW), wet weight (Weg), length (LpL) and height (LpH) of left chelar propodus and right chelar propodus length (RpL). The population was divided into six size classes. Morphological and statistical evidence, suggesting that intersex individuals may function as males, were recorded. Sexual dimorphism was indicated in all the measured dimensions. The overall sex ratio was 1:2.3, in favor of females. Typical males showed higher abundance percentage in the larger size classes than in the smaller ones. Typical females showed the reverse. Intersex individuals were present only in size classes that included typical females. That finding indicates a connection between their role and typical females abundance, suggesting that they may serve as a “backup” for typical males in reproduction. That, in turn, could interpret their higher abundance percentage in C. latens population (≈10.4%) than in other hermit crabs populations, assuming a higher males mortality rate in the case of the C. latens population.
https://ejabf.journals.ekb.eg/article_32245_ab578b7c1ad2fbe40591237e8dcb0d21.pdf
2019-05-18
397
409
10.21608/ejabf.2019.32245
Calcinus latens
Diogenidae
Dimorphism
Intersexuality
Aqaba Gulf
Obuid-Allah,
A. H.
1
AUTHOR
Abd El-Wakeil,
K. F.
2
AUTHOR
Al-Hammady,
M. A. M.
3
AUTHOR
Said,
E. S. A.
4
AUTHOR
ORIGINAL_ARTICLE
Biochemical changes and economical estimation of salted sand smelt fish (Atherina boyeri)
The current investigation was planned to investigate the biochemical changes and economical estimation of salted Sand smelt fish (Atherina boyeri). Dry salting levels; 10, 15, 20 and 25% were applied. Results showed that the raw whole fish composed 75.49% moisture, 13.02% crude protein, 1.83% lipid and 9.60% ash content. pH value and thiobarbituric acid (TBA) values were 6.35 and 0.88 mg Malonaldhyde (MA)/ kg sample, respectively. Concerning the bacterial load; total plate count (TBC) and Hallophillic bacterial count (HBC) were 2.48 and 2.00 log10 cfu/g samples, respectively. All of these values changed as affected by salt concentration used and storage period. Sensory scores were higher in heavy salted products (20% and 25% salt) than light salted products (10% and 15% salt). Economically, fish salting is a simple technique can be created new working chances (small projects) for many people and also it can be reduced of fish protein gap.
https://ejabf.journals.ekb.eg/article_32441_3379d7e93c2c305d2d7425a6f754c0d0.pdf
2019-05-20
411
419
10.21608/ejabf.2019.32441
Sand smelt fish
Atherina boyeri
salted fish
Biochemical changes
Quality indices
economical estimation
Ibrahim,
S.M.
1
AUTHOR
Mohamed,
S.M.
2
AUTHOR
Ibrahim,
M. A.
3
AUTHOR
ORIGINAL_ARTICLE
Experimental approach for improving the removal efficiency of a metal pollutant using the Activated Sludge as a wastewater treatment process
The objective of this study was to investigate the feasibility of developing improved activated sludge cultures to remove a heavy metal salt (CuSO4.5H2O) present in high concentrations.Two types of bioreactors were employed to develop and maintain the aerobic cultures used to conduct the heavy metal-uptake studies. These were continuously stirred-tank batch reactors (CSTBR's) that used to sustain the aerobic cultures at selected specific growth rates beside detecting metal tolerant species and completely mixed batch reactors used to conduct metal uptake studies with cultures derived from the CSTBR's. Copper was selected for this application. The aerobic continuously stirred batch reactor (ACSBR) of high growth rate (HGR) exhibited good metabolic activities as indicated by the values of PH, dissolved oxygen, TSS, VSS, , BOD and COD belonging to the mixed liquor provide an evidence for the presence of metabolically active organisms. The data obtained in the present study established that the copper uptake by the test sludge in the different unit reactors at the first four hours ranged from 15.63% to 27.21%. The present study proved that the maximum removal percent of copper salt achieved 79.6% to 81.9% of the total percent of copper uptake, while those obtained by the control experimental sludge ranged from 0.20% to 2.10%. This means that there was a big difference among the averages of metal uptake.
https://ejabf.journals.ekb.eg/article_32839_d41d8cd98f00b204e9800998ecf8427e.pdf
2019-05-22
421
432
10.21608/ejabf.2019.32839
Heavy metals
Activated sludge
Copper sulphate
Wastewater treatment
protozoa
Bacteria
Mansor
Galal
1
AUTHOR
Ahmad
AbdElazez
2
AUTHOR
Ramadan
M. Abd-Rabuo
3
AUTHOR
Atef
El-Tokhy
4
AUTHOR
ORIGINAL_ARTICLE
Morphometric and Meristic features and Length-Weight Relationship as indicators of quality of Brycinus macrolepidotus in Lower River Ogun, Nigeria
One Hundred And Eighty samples of True big-scale fish (Brycinus macrolepidotus Valenciennes, 1850) were collected bi-monthly from June – November, 2017, from River Ogun, Nigeria. Total length and body weight of all samples measured and samples were grouped based on length into sizes: juveniles (10-14.9 cm), sub-adults (15.1-19.9 cm), and adults (20.2-26.3 cm). Significant differences (p<0.05) were observed in the meristic characters in all the groups. Dorsal fin ray ranged from 7-9, pelvic fin ray 8-12, caudal fin ray 18-24, pectoral fin ray 7-12, anal ray 12-14 and dorsal spine 0-9 through the wet and dry season for all sizes. Length-Weight relationship for juveniles, sub-adult and adult sizes showed ‘a’, ‘b’ and ‘r’ values for juveniles as -0.82, 2.17 and 0.85, sub-adult as -0.38, 1.82 and 0.90 and adult as 0.44, 1.03 and 0.78 respectively. Mean condition factor recorded was 1.16±0.15, 1.39±0.13, and 2.17±0.59 for adult, sub-adult and juvenile respectively. Conclusively, B. macrolepidotus in this aquatic ecosystem could be in a good environment and quality as condition factor of all sizes were above 1.
https://ejabf.journals.ekb.eg/article_33777_acaeadfd9e4be7dfb09b214a58e58fc3.pdf
2019-06-01
433
441
10.21608/ejabf.2019.33777
Brycinus macrolepidotus
River Ogun
Nigeria
Morphometry
Meristic features
Adeosun
F. Idowu
1
AUTHOR
Idowu
A. Adedoyin
2
AUTHOR
Oghenochuko
M. Titilayo
3
AUTHOR
Adebowale
A. Quddus
4
AUTHOR
Adekoya
O. Micheal
5
AUTHOR
ORIGINAL_ARTICLE
Factors Affecting Fish Blood Profile: B- Effect of Environmental and Genetic Factors
Continued studies were conducted to understandhow some factors could affect blood composition of a fish. In this part (B) of the present serial studies, we gave light on the effect of some environmental and genetic factors on hematological and /or the biochemistry parameters of a fish. These factors are water temperature, narcosis, handling stress, poly-culture, farm conditions, starvation, fish species, sex, and size.
https://ejabf.journals.ekb.eg/article_33852_b52dcaea17b903eb119a67e30e240ff3.pdf
2019-06-05
443
459
10.21608/ejabf.2019.33852
Environmental factors
Genetic factors
Haematology
biochemistry
Bood profile
Temperature
Starvation
Abdelhamid
M. Abdelhamid
1
AUTHOR
Mohamed
M. Refaey
2
AUTHOR
Mahmoud
F. Salem
3
AUTHOR
Mostafa
A. M. El-Kattan
4
AUTHOR
ORIGINAL_ARTICLE
Environmental Characteristics of the Egyptian Mediterranean Coast
In an attempt to evaluate the environmental quality of the Egyptian Mediterranean Sea from El-Dabaa to El-Saloum, environmental parameters as well as amino acids, petroleum hydrocarbons, pesticides and PCBs have been studied in water and sediment samples from seven different locations during the year 2014. Spatial and temporal fluctuations as well as statistical analysis between the analyzed variables were examined.
The results demonstrated a wide range of water temperature (13.50-29.00°C), slightly narrow fluctuations in salinity (37.20-38.78), and well-oxygenated seawaters (6.40–8.96 mg O2/l), relatively low amounts of oxidizable organic matter (0.10-1.60 mg O2/l) and the seawater was slightly alkaline (pH range 8.04–8.63). Nutrient characteristics sustained the levels up to 2.45, 5.42, 5.40, 0.28 and 5.36 µM for reactive phosphates, reactive silicates, ammonium, nitrite and nitrate, respectively.
These obtained results signified that water quality from El-Dabaa to El-Saloum is good; within the permissible limits in the majority of monitoring sites, and all stations were generally between Oligotrophic and mesotrophic states, characterized by a relatively low to medium level of nutrients.
Furthermore, seawater from the most sites did not show any levels of pesticides, PCBs and petroleum hydrocarbons. These levels, if any, may have been much lower than the detection limit for the compounds analyzed. In marine sediments, the concentrations of amino acids demonstrated a range of 1884.5-19569.6 µM, while pesticides, PCBs, and petroleum hydrocarbons were below detection limit to 3086, 120, 145 ng/g; respectively. These results are much lower in sediment of the western part of the Egyptian Mediterranean coast compared to other sites and also much lower than permissible levels given by National Academy of Sciences and National Academy of Engineering.
https://ejabf.journals.ekb.eg/article_34027_c5226045f03b2e51c59c1e328042f542.pdf
2019-06-08
475
490
10.21608/ejabf.2019.34027
Mediterranean Sea
environment
Amino acids
PHs
Pesticides
PCBs
Egypt
Naglaa
A. El-Naggar
1
AUTHOR
Laila
A. Mohamed
2
AUTHOR
Ahmed
Abdel-Halim
3
AUTHOR
Hosny
E. Emara
4
AUTHOR
ORIGINAL_ARTICLE
Impact of Copper and Cadmium on the Nutritional Value of the Rotifer Brachionus plicatilis and their Effect on Dicentrarchus labrax Fish Larvae.
Brachionus plicatilis is a rotifer that considered an important live feed in aquaculture for feeding the initial stages of many fish larvae. This study aimed to determine the growth, survival rate and biochemical alterations of Dicentrarchus labrax (sea bass) larvae fed on B. plicatilis treated with sublethal concentrations of cadmium chloride (CdCl2) and copper sulphate (CuSO4). This study is extended to evaluate the alteration in the nutritional value of B. plicatilis upon the exposure to sublethal doses of these heavy metals. The results showed that the total length and width means of D. labrax larvae reared on B. plicatilis exposed to CuSO4 and CdCl2 increased significantly when compared to their control (p< 0.05.). D. labrax larvae fed on rotifers treated with CuSO4 and CdCl2showed a significant decrease in their survival percentages (p < 0.001) and their total lipids and protein levels (p < 0.001) when compared to their control. Moreover, the level of carbohydrate and lipid decreased significantly (p < 0.05) in the B. plicatilis exposed to sublethal concentration of CdCl2 and CuSO4. Most of the essential and nonessential amino acids in B. plicatilis exposed to these heavy metals were partially decreased while methionine, phenylalanine, histidine and leucine showed significant increase. Treating B. plicatilis with CdCl2 showed a significant decrease in mono and poly unsaturated fatty acids. The oleic acid ((C20:1 ω9) and the caprylic acid (C8:0) decreased in B. plicatilis exposed to CdCl2 and CuSO4, while the Eicosatrienoic acid (C 20:3ω3) and the Eicosapentaenoic acid (C 20:5ω3) were not detected. The Arachidonic acid (C20:4 ω6) decreased significantly in treated B. plicatilis with CdCl2. The present work concluded that, D. labrax larvae feeds on B. plicatilis exposed to sublethal concentration of Cd and Cu heavy metals showed marked effect on the survival rate and biochemical composition.
https://ejabf.journals.ekb.eg/article_33919_76f9dcda2cda1453f3582910659c44bb.pdf
2019-06-04
491
503
10.21608/ejabf.2019.33919
Brachionus plicatilis
Dicentrarchus labrax
Heavy metals
cadmium
copper
Nutritional Value
biochemical parameters
Heba
S. El-sayed
1
AUTHOR
Gihan
M. El-khodary
2
AUTHOR
ORIGINAL_ARTICLE
Factors Affecting Fish Blood Profile: A- Effect of Nutritional Treatments
Blood profile was studied as affected by different fish species, dietary crude protein, replacements, and additives, as well as diets' types, in laboratorial and field studies. It was clear that most studied treatments (food types, probiotic levels, levels of clover seed wastes, initial body weight, and Teen Barshomy wastes and levels) had been significantly affected most studied haematological and/or biochemical parameters. That means that fish blood constituents are not stable within known ranges, but widely varied according to various environmental conditions.
https://ejabf.journals.ekb.eg/article_33741_1bf38810a04c8cf0bc74d48f9f8ac934.pdf
2019-06-01
517
526
10.21608/ejabf.2019.33741
fish
Haematology
biochemistry
nutrition
Diet type
probiotics
Abdelhamid
M. Abdelhamid
1
AUTHOR
Mohamed
M. Refaey
2
AUTHOR
Mahmoud
F. Salem
3
AUTHOR
Mostafa
A. M. El-Kattan
4
AUTHOR
ORIGINAL_ARTICLE
First record for the sunfish Mola mola (Molidae: Tetradontiformes) from the Egyptian coasts, Aqaba Gulf, Red Sea, with notes on morphometrics and levels of major skeletal components
The sunfish Mola mola is recorded for the first time from the Egyptian waters at Abu Galum Protectorate Area (South Sinai), Aqaba Gulf, Red Sea. The present study conducted to give information on morphometric characters, anatomy and levels of major elements in skull, vertebrae and paraxial parts of its skeletal system. These elements comprised Ca, P, Na, K, Mg, S, Cl, Zn and Cu and their levels were estimated. The results exhibited that Ca and P were the main components in skull and were the most dominant elements in paraxial skeleton in addition to remarkable ratios of Na and Cl. Cu was detected with very low ratios only in paraxial skeleton parts. The annuli in examined vertebrae were counted and showed that, this fish may be in its second age group.
https://ejabf.journals.ekb.eg/article_33933_34e08147957d4b9e4c2e63a8f6d0fd82.pdf
2019-06-03
563
574
10.21608/ejabf.2019.33933
Sunfish
Mola mola
Abu Galum
South Sinai
Red Sea
Aqaba Gulf
Morphometrics
Mohamed
A. Amer
1
AUTHOR
Ahmed
El-Sadek
naser12_7@yahoo.com
2
AUTHOR
Ahmed
Fathallah
fathallah74@yahoo.com
3
AUTHOR
Hamdy
A. Omar
4
AUTHOR
Mohamed
M. Eltoutou
5
AUTHOR
ORIGINAL_ARTICLE
Improvement of spawning in Siganus rivulatus: the impact of different LHRHa doses, spawning methods and sex ratio on spawning performance and egg quality
Induced spawning is used to solve failure of complete oocyte maturation and fish spawning in captivity. Hormonal inductions with luteinizing hormone releasing hormone analogue (LHRHa) were examined to induce synchronized ovulation and improve spawning of wild-caught rabbitfish, Siganus rivulatus. Three experiments were carried out to study their impact on egg quality. Experiment I: LHRHa intramuscular injections of 50 and 100 µg kg-1 were used for two different groups respectively with a third control group. Experiment II: LHRHa injection (100 µg kg-1) was used to study the impact of both spontaneous spawning and stripping methods. Experiment III: LHRHa injection (50 µg kg-1) was used to study the influence on sex ratios. Two successive injections (LHRHa) were used for females in all experiments while male fish received only one dose. In experiment I, the response to higher dose showed a significant increase (p < 0.01) in egg diameters, latency time, total number of spawning fertilized eggs and hatching rate. In experiment II & III, a significant improvement (p < 0.01) was observed in all egg quality parameters with both spontaneous spawning and sex ratio (2 male:1 female). LHRHa injection is an adjunct to improve spawning performance and Siganus rivulatus culture production.
https://ejabf.journals.ekb.eg/article_34456_706b97998ac6a15b2ce2a4cfddafe84b.pdf
2019-06-12
505
515
10.21608/ejabf.2019.34456
Siganus rivulatus
Induce spawning
LHRHa
Spontaneous spawning
Manual stripping
egg quality
Amal
F. Fahmy
1
AUTHOR
ORIGINAL_ARTICLE
Effect of climate change on the reproduction pattern of sea urchin Echinometra mathaei at the Gulf of Suez, Red Sea, Egypt.
The reproduction patterns of E. mathaei's population at the Gulf of Suez were investigated during two periods, the first was 1992-1993 and the second was 2015-2016. The effects of elevating temperature during these periods on reproduction periodicities were studied. The surface water temperature showed an increase during the period 2015-2016 by 1.36 oC at summer and 1.24 oC at winter from that recorded during 1992-1993. In 1992-1993, individuals of E. mathaei had one definite lengthy spawning season, which were expressed by main spawning period from June to August with maximum gonadal activity in July. On contrary, there was an obvious shift at the spawning season during 2015-2016; individuals of E. mathaei exhibit more than one breeding season. However, they are resting only during December, January, February, May and September. They showed four spawning seasons, the first from October to November, 2015, the second from March to April, 2016; the third from June to August, 2016, and the fourth from October to November, 2016. The overall sex ratio (males to females) during the first period (1992-1993) of the current study was 1:2.5, while in 2015-2016, it recorded an average male to female of 1:2.4. Size at sexual maturity decreased from 1992-1993 (2.25 cm for females and 2.03 cm for males) to 2015-2016 (1. 8 cm for females and 1.5 cm for males). The study recorded a positive relationship between fecundity and size. The oocyte/female was highest in 1992-1993 (23 X 105), decreased during 2015-2016 (11.2 X 105). Seasonal changes were observed in maturity stages related to spawning season at both periods of study. In the first period (1992-1993), most stages were represented with fluctuation in their percentage. The data collected showed that all stages showed one pattern of increasing and decreasing according to the stage until May, 1993 and subsequently, the pattern changed between the stages during the period from May to August. On the other hand, during the second period (2015-2016) all maturity stages were represented but in alternative states between the months of study.
https://ejabf.journals.ekb.eg/article_35918_c1b9d7fb9cbcab404740006138c83e89.pdf
2019-06-20
527
544
10.21608/ejabf.2019.35918
Echinometra mathaei
Reproduction
Global Warming
Temperature
Gulf of Suez
Mohamed
Hamza Hasan
1
AUTHOR
ORIGINAL_ARTICLE
The consumption rate of three zooplankton species of different size fed on the green microalgae Chlorella vulgaris
The live food (phytoplankton and zooplankton) is a very important food source in fish farming, particularly for early larval stages. Therefore, the present study was carried out to determine the consumption rate (grazing (G) and ingestion (I) rates) of three zooplankton species of different size, Brachionus plicatilis, Acanthocyclops trajani, and Heterocypris salina. Five different concentrations (10, 20, 30, 40 and 50 x104 cells/ml) of the most common cultured phytoplankton species (Chlorella vulgaris) were utilized as fed for these species. The optimal concentration of the green algae that nourish the zooplankton species with minimum leftover that could affect the water quality was determined. The results revealed that grazing and ingestion rates of B. Plicatilis and H. salina showed linear regression with algal concentrations (p < 0.008; R2=0.72 and 0.82,) and (p <0.001; R2= 0.969 and 0.964), respectively.Their consumption rates values increased markedly with increasing the concentration of C. vulgaris. On the other hand, the consumption rate of A. trajani were independent of algal concentrations (p ≥ 0.09 and 0.07; R2= 0.07 and 0.05, respectively), and reached their maximum values at 30x 104 cell /ml. The study concluded that C. vulgaris is a suitable food for B. Plicatilis and H. salina while it is not for A. trajani, where its feeding behaviour still needs more in-depth studies.
https://ejabf.journals.ekb.eg/article_35001_7869cea998818812a241a98d8d8aec29.pdf
2019-06-16
575
583
10.21608/ejabf.2019.35001
Zooplankton Phytoplankton
Chlorella vulgaris
Grazing rate
Ingestion rate
Live food
Mahmoud
H. Hegab
1
AUTHOR
Shymaa
S. Zaher
2
AUTHOR
Hesham
R.A. Mola
3
AUTHOR
ORIGINAL_ARTICLE
The first data on the population parameters of the main fish species in man-made Lake Buyo (River Sassandra, Côte d'Ivoire)
The growth parameters and the exploitation rate of the main 9 fish species found in the lake of the Buyo dam was studied from July 2017 to June 2018. The monthly frequency data for lengths obtained from experimental and commercial fisheries were analysed using the FiSAT II software and its sub-program ELEFAN for the evaluation of population parameters. The asymptotic length (L∞) varies from 63.33 cm for Chrysichthys nigrodigitatus to 15 cm for Synodontis koensis. The specific growth rate (K) is higher (0.9 year-1) in Malapterurus electricus and lower (0.21 year-1) in the species Schilbe mandibularis, while the growth performance index (φ') calculated from 3.48 in Chrysichthys nigrodigitatus to 2.04 in Tilapia zillii. The fishing mortality (F) and exploitation rate (E) calculated are below the optimal exploitation rate for most species. The recruitment pattern is a continuous model with a single peak and reveals a recruitment period in the months of May, June, August and October. According to the exploitation predictions, the species O. niloticus, M. electricus, C. nigrodigitatus, D. rostratus and L. niloticus have an exploitation rate E respectively 0.35; 0.46; 0.27; 0.41 and 0.31 higher than the optimum exploitation rates Eop (respectively 0.29; 0.33; 0.37; 0.28 and 0.27), which would mean that the stock of these species in the fishery is already reduced to more than 50% of its untapped biomass; therefore overexploited. Our study provides the first data on the population parameters of the main fish species from the Lake Buyo. These data could contribute to establish a sustainable management plan for fisheries resources only in lake Buyo.
https://ejabf.journals.ekb.eg/article_34788_780a78ae1bf193da2409306a13374f0c.pdf
2019-06-10
585
597
10.21608/ejabf.2019.34788
River Sassandra
Lake Buyo
Côte d’Ivoire
West Africa
growth parameters
fishery management
Goli Bi
B. E. P.
1
AUTHOR
Kamelan
T. M.
2
AUTHOR
Berté
S.
3
AUTHOR
Kien
K. B.
4
AUTHOR
Ndiaye
S.
5
AUTHOR
Kouamelan
E. P.
6
AUTHOR
ORIGINAL_ARTICLE
Population dynamics and fisheries characteristics of the Blue Crab Callinectes sapidus (Rathbun, 1896) as an invasive species in Bardawil Lagoon, Egypt
Population parameters of the blue crab, Callinectes sapidus were investigated based on the data collected from the commercial catches of Bardawil Lagoon covering 20 months during the fishing seasons 2016-2018. Growth parameters were estimated from the carapace length frequency distribution data of more than 1960 specimens. The von Bertalanffy growth parameters for males, females and sexes combined were K= 1.53, 1.45 and 1.42 y-1 respectively, L¥= 9.71, 10.11 and 9.96 cm respectively and to= -0.14, -0.15 and –0.15 year respectively. The values of total mortality coefficient (Z), natural mortality coefficient (M) and fishing mortality coefficient (F) were 5.19, 2.31 and 2.88 y-1 for males, 6.21, 2.51 and 3.70 y-1 for females and 6.35, 2.57 and 3.78 y-1 for sexes combined respectively. Exploitation rate was estimated at 0.55, 0.60 and 0.59 for males, females and sexes combined respectively. The age-based yield per recruit analysis showed that the blue crab stock is being exploited at its optimum limit. The present study suggested that this fishery resource could be expanded with the increase of current fishing effort but such increasing is not acceptable because the commercial species in the lagoon are over exploited. The current management regulations must be revised and improved to sustain and optimize yields.
https://ejabf.journals.ekb.eg/article_34459_8479065ad8558f516d838300fea590c6.pdf
2019-06-12
599
611
10.21608/ejabf.2019.34459
Bardawil Lagoon
Callinectes sapidus
Portunidae
Population dynamics
Management
Egypt
Sahar
F. Mehanna
1
AUTHOR
Mohammed
G. Desouky
2
AUTHOR
Alam
E. Farouk
3
AUTHOR
ORIGINAL_ARTICLE
A study on growth, mortality and a biochemical growth indicator of Oreochromis niloticus in a Nile Canal, Egypt.
This study was carried out to examine growth and mortality and the efficiency of the ratio of RNA/DNA in characterizing growth of Oreochromis niloticus. Growth in weight with length of fish was predicted by the significant relationship: Log W (g) =-1.6696 + 3.1971 log L (cm). The value of the slope of that relationship was found to be the highest, when compared with other reported values in different localities, where rise in temperature in the period of study is suspected. The fish under study were found to fall in 5 age-groups. Seasonal variation of the condition coeffecient “K” showed a slight decrease on going from winter towards summer. The instantaneous total mortality was predicted as 1.4022, and consequently survival rate was 0.2461 and mortality rate was more than 75 %. The lower value of survival rate than that earlier reported study, was thought to be due to rise in temperature because of global warming. The ratio of RNA/DNA increased with increase of either age or length of fish, but declined at age V, corresponding to the length of 24 cm. This finding could be attributed to the effect of fishing recruitment which is covering the first 4 age-goups. Significant relationship between RNA/DNA and K ( r 2 = 0.78), then with seasonal variation ( r 2 = 0.81). Those relationships showed a rise in the molecular ration, and K, during winter and decline in summer could be speculated as a compensation in growth after disturbance in growth during spawning period. Thus, the RNA/DNA ration variation could be tied to describing growth of O. niloticus, as well as seasonal variation effects.
https://ejabf.journals.ekb.eg/article_48321_3bc2340827367bbedef30cf6978c5e20.pdf
2019-04-28
639
656
10.21608/ejabf.2019.48321
Oreochromis niloticus
Molecular bioindicators
Growth
mortality
RNA/DNA
River Nile
Khallaf
, E.A.
1
AUTHOR
Alne-na-ei,
A. A.
2
AUTHOR
El-Garawani,
I.M.
3
AUTHOR
Elgendy,
R. G.
4
AUTHOR