ORIGINAL_ARTICLE
COMPARATIVE STUDY ON THE RABBIT FISHES SIGANUS CANALICULATUS INHABIT THE ARABIAN GULF AND SIGANUS RIVULA TUS INHABIT THE RED SEA IN SAUDI ARABIA
The present work was carried out to compare some biological aspects, chemical composition, fatty acids, minerals and heavy metals of the commercial Rabbit fishes Siganus canaliculatus inhabit the Arabian Gulf and Siganus rivulatus inhabit the Red Sea in Saudi Arabia. Fish samples were selected to represent all fish size categories in the catch and were collected during the period from November 2002 to March 2003. Each fish specimen was measured, weighted and recorded, then dissected to investigate the fullness of alimentary canal and the maturity stage of the gonads. The length-weight relationships were computed by simple regression analysis and graphically represented. Both of absolute and relative condition factors (Kc and Kn) were estimated and graphically represented. The muscles (flesh) were removed and weighted to estimate the flesh percentage. Samples of flesh were taken to determine moisture, protein, ether extract, ash, fatty acids, minerals and heavy metals. Some samples of the entire fish were used to proximate analysis. The present results indicated that the Kc, Kn, maximum total length and flesh percentage of Rabbit fish Siganus canaliculatus were'higher than that of Siganus rivulatus. In general, polyunsaturated fatty acids were relatively low and heavy metal concentrations in the flesh of the two Siganus species-were lower than the permit limits for Human consumption,
https://ejabf.journals.ekb.eg/article_1782_8e7d3dab3f0c09a6a15cb515e5b0fa81.pdf
2003-09-01
1
19
10.21608/ejabf.2003.1782
fish
Siganus
Arabian Gulf
Red Sea
biology
minerals
Heavy metals
fatty acids
Adel
Tharwat
1
Depart, of Aquatic Res. Fac. of Agric. Sciences and Food King Faisal University, PO Box 420, Hofuf, 31982 Saudi Arabia
AUTHOR
Mohammed
Al-Owafeir
2
Depart, of Aquatic Res. Fac. of Agric. Sciences and Food King Faisal University, PO Box 420, Hofuf, 31982 Saudi Arabia
AUTHOR
ORIGINAL_ARTICLE
NEUROSECRETORY CELLS IN SOME PRINCIPAL GANGLIA OF THE SEA-HARE, APLYSIA OCUHFERA
The principal ganglia of the central nervous system (CNS), of the sea hare, Aplysia oculifera, are concentrated around the oesophagus, caudally from the buccal mass. The usual complement of such ganglia comprises paired cerebral, buccal, pleural and pedal ganglia. The neurosecretory cells (NSCs) were variably observed in all the above mentioned ganglia. They were differentiated according to size and shape into three cell types namely: large (type A), medium (type B) and small (type C) cells. The A cell type is less common and has a large size with an oval or flattened shape. It measures from 116.6 x 69.9 \i to 34.8 x 19.5 |i, with a centrally located irregularly shaped or polymorphic nucleus, with many small intensely colored nucleoli. The B cell type is more numerous and has an oval or rounded shape, with a rounded and centrally located nucleus, which has many small intensely colored nucleoli. It measures from 59.5 x 40.8 [x to 22.2 x 18.1 ji. Meanwhile, C cell type constitutes the majority of the NSCs. It is small rounded or ovoid cell, measuring from 35.6 x 31.5 \x to 5.6 x 5.0 \x, with a large rounded nucleus, having a sharply defined eccentrically placed nucleolus.The ultrastructure of NSCs of these ganglia was also investigated. Generally, it showed active Golgi body, rough endoplasmic reticulum and a great number of free ribosomes. The electron dense neurosecretory granules were observed. These were attributed to the activity of both Golgi apparatus and rER that have the main role in their formation.
https://ejabf.journals.ekb.eg/article_1783_81dd2c5a997f972ee68d1b7c85fc3f3d.pdf
2003-09-01
21
44
10.21608/ejabf.2003.1783
Aplysia
Opithobranchia
anatomy
histology
Ultrastructure
neurosecretion
nervous system
Setaita
Slecm
1
Department of Zoology, Faculty of Science, Ain Shams University
AUTHOR
ORIGINAL_ARTICLE
HEALTH RISK ASSESSMENT IN RELATION TO HEAVY METALS POLLUTION OF WESTERN MEDITERRANEAN SEA, EGYPT
Concentrations of heavy metals (Mn, Fe, Zn, Cu, Pb, Co, Cr, Ni and Cd) were measured in the sediment collected from 7 stations along west Mediterranean Sea (Sidi- Krir ; open sea , east & west Sidi Krir, Sidi Barrani and El-sallum 1,2, 60m). Also, health risk has been performed in relation to recreational activities at these places, using an exposure assessment model. The results of this study indicated a general moderate or absence of serious pollution in these places due to heavy metals; whereas the concentration of elements found could be mainly due to geological and atmospheric sources. However, the health risk evaluation is useful as a screening methodology for assessing the urgency of sediment remediation actions.
https://ejabf.journals.ekb.eg/article_1784_f3a8acabd41d3896a264821b20f92d1d.pdf
2003-09-01
47
66
10.21608/ejabf.2003.1784
Heavy metals
Risk assessment
Mediterranean Sea sediment pollution
Amany
Sikaily
1
Environmental Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt
AUTHOR
ORIGINAL_ARTICLE
ASSESSMENT OF HEAVY METAL POLLUTION IN SURFACE MUDDY SEDIMENTS OF LAKE URULLUS,SOUTHEASTERN MEDITERRANEAN, EGYPT
The concentrations of certain heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pd and Zn) in the total and labile fractions of muddy sediment samples collected from eleven sites in Lake Burullus in January 2003 were investigated in order to evaluate the pollution status of the Lake. The metal contents were determined by means of Atomic Absorption Spectrophotometer (AAS) using MESS-2 certified reference material (National Research Council of Canada). The average concentrations of the heavy metals analyzed in total sediment fractions exhibited the following decreasing order: Fe > Mn > Cu > Ni > Zn > Cr > Pb > Co > Cd, while the average concentrations of the heavy metals analyzed in the labile fraction followed the order: Fe > Mn > Cu > Ni > Pb > Zn > Co > Cr > Cd. The concentrations of all studied heavy metals ranged between the Effect Range-Low (ERL) and the Effect Range-Median (ERM) for most studied locations. Metal pollution index (MPI) shows very high values for both total and labile fractions at all the examined locations. Field observation reveals that Lake Burullus received industrial, agricultural and domestic sewage, suggesting that anthropogenic input is the main source of heavy metal contamination. Health hazard calculations for the contaminated sediments exhibited a possibility,pf health risk due to long-term exposure of the human to the polluted sediments of Lake Burullus.
https://ejabf.journals.ekb.eg/article_1785_47fc78404e173070e518910f9bf21c8c.pdf
2003-09-01
67
90
10.21608/ejabf.2003.1785
Heavy metals
Sediment
labile
dermal contact
pollution
Lake Burullus
Egypt
Ahmed
El Nemr
1
Environmental Dept. National Institute of Oceanography and Fisheries, Alexandria, Egypt
AUTHOR
ORIGINAL_ARTICLE
ZOOPLANKTON COMMUNITY OF THE EGYPTIAN MEDITERRANEAN COAST
The zooplankton community during the present study ( autumn 2000 - winter 2001 ) appeared to has low diversity as compared to that found in the whole Egyptian Mediterranean waters. The western side was characterized by higher diversity than the eastern side, which is affected by land based effluents entering the sea from the coastal lakes and the River Nile. The standing crop values indicated poof zooplankton population in the whole study area. The dominance of species showed drastic changes as compared to the previous studies, whereas the previously dominant species are ranked recently as rare species. The identified species exhibited different seasonal patterns of vertical distribution but the majority of them were recorded in the subsurface layer down to 200 m depth.
https://ejabf.journals.ekb.eg/article_1786_acbfdea795f86417cf4c6f13643c9b38.pdf
2003-09-01
91
108
10.21608/ejabf.2003.1786
zooplankton- Egyptian Mediterranean Coast
Vertical distribution
Nagwa
Abdel-Aziz
1
National Institute of Oceanography and Fisheries, Alexandria, Egypt
AUTHOR
Sawsan
Aboul-Ezz
2
National Institute of Oceanography and Fisheries, Alexandria, Egypt
AUTHOR
ORIGINAL_ARTICLE
INTENSITY AND DENSITY OF PYIGIDIOPSIS SUMMA AND GENETA, AND THEIR EFFECT ON SOME OF THE SERUM CONSTITUENTS OF TILAPIA SP.
Parasitic infections of Tilapia sp. from the agricultural drainage in the eastern region of Saudi Arabia were examined. The identification of the parasites, the histopathological, and the histo-chemical analysis of the infected fish organs were investigated in series of studies. The present work estimated the intensity and the density of the encysted metacercariae of the Pyigidiopsis summa and geneta in random samples of different fish organs. The study also investigated the effect of these parasites on the serum glucose, total protein, and the activity of the LDH enzyme. The results indicated that all fish in the examined samples (100%) were infected with the encysted metacercariae of the Heterophyd sp. The highest intensity of the metacercariae was found in the liver (43.30%), and its mean density was 17.31±35.68 cyst/cm2. The intensity of the metacercaria in the intestine was 33.37%, and its mean density was 13.34±15.77 cyst/cm2. The lowest intensity and density of the parasite was in the skin, 23.33%, and 9.33±8.87 cyst /cm2 respectively* The biochemical analysis showed a significant increase in the serum glucose, and the activity of the LDH enzyme of the infected fish. However, total protein did not vary much between the infected fish and the uninfected control.
https://ejabf.journals.ekb.eg/article_1787_7d4743c20ba17b58442c468eca6e3c58.pdf
2003-09-01
109
124
10.21608/ejabf.2003.1787
fish environment
bio-pollution
parasites intensity
biochemical parameters
Tilapia sp
Fatma
Elnemaki
1
Biology Department, Faculty of Science, king AbdulAziz University, Jeddah, Saudi Arabia.
AUTHOR
ORIGINAL_ARTICLE
ECONOMICAL EVALUATION OF NILE TILAPIA (OREOCHROMIS NILOTICUS) CULTURE IN RICE FILEDS RECEIVING VARYING FEEDING INPUTS
This study aimed to evaluate the nutritive value, growth parameters, productivity and economical efficiency of dried blue green algae for feeding Nile tilapia (Oreochromis niloticus) cultured in rice fields. Eight rice fields (one fed. each) were cultivated by rice and divided into four groups, the first group (Ti) of two fields with dried blue green algae at a rate of 5% of body weight per week, and the second (T2) one was fed on artificial diet containing 20% crud protein. The third group (T3) was fed on natural food resulting from fertilization only (30 Kg. chicken manure/week) and the fourth group (T4) has served as a control (without any treatment).All fields were stocked with Nile tilapia (1000 fingerlings/fed.) with an average weight of 30 g. The growing season for tilapia fish was 90 days. Results obtained can be summarized in the following:- Treatments applied had sigpificant effect on final body weight, specific growth rate, body length and condition factor of Nile tilapia.- The highest survival rate was obtained with group of fish on T2 followed by Ti^T* treatments respectively- The highest net production was recorded with group offish on T2 followed by Ti, T3 and T4 treatments respectively.- The highest net return was recorded with group of fish on Ti followed by T3, T2 and T4 treatments respectively.
https://ejabf.journals.ekb.eg/article_1788_90f2910894bdf41998235102b926466d.pdf
2003-09-01
125
140
10.21608/ejabf.2003.1788
Nile tilapia
productivity
rice fish culture
Blue green algae
economical efficiency
Abdel
Salama
1
Department offish Economic.Central Laboratory for Aquaculture Research at Abbassa, Sharkia Governorate, Egypt.
AUTHOR
ORIGINAL_ARTICLE
COMPARATIVE FECUNDITY ESTIMATION OF CULTURED FEMALE FISHES WITH MYXOSPOREAN AND FUNGAL INFECTIONS
This is the first trial so far in Egypt through upgrading the fish production by revealing specific ovarian myxozoan and fungal infections that affect their fecundity. Fecundity comparison was held between noninfected and infected Oreochromis niloticus and Cyprinus carpio with either Myxobolus spp. or Icthyophonus hoferi.The myxosporean parasite, Myxobolus spp. (with higher prevalence in both species ) appeared as numerous white large rounded spores embedded in the ovarian tissue causing excessive fibrosis. The fungus /. hoferi inspite of its lower prevalence among the 2 species, the white capsulated cysts and separate colonies caused ovarian atresia and fibrosis with inflammatory oedema.Drop of fecundity was represented by significant decrease in relative and absolute fecundity, total protein, gonadosomatic and hepatosomatic indices ranging from highly with 0. niloticus to moderately with C carpio. In O. niloticus ( more sensitive ) /. hoferi reflected a positive correlation with ovarian weight and gonadosomatic index, while Myxobolus spp. recorded a negative correlation between ovarian weight and the body parameters (weight, length & depth).The results revealed that infection takes place among apparently healthy fish proving that gonadal periodical examination is the only way for detecting such infections.
https://ejabf.journals.ekb.eg/article_1789_7e66027f8ec612849de4c6a5b0f2c34a.pdf
2003-09-01
141
165
10.21608/ejabf.2003.1789
Fish fecundity
Ovary
Icthyophonus hoferi
Myxoholus sp
Nahla
Elkhatib
1
Department of Fish Disease, Animal Health Research Institute, Dokki, Giza.
AUTHOR
Nashwa
Elias
2
Department of Fish Disease, Animal Health Research Institute, Dokki, Giza.
AUTHOR
ORIGINAL_ARTICLE
HISTOLOGICAL AND ULTRASTRUCTURAL OBSERVATIONS ON GILLS OF TILAPIA NILOTICA L. (OREOCHROMIS NILOTICUS) IN LAKE QARUN, FAYOUM PROVINCE, EGYPT
Fish productivity has been affected by a progressive increase of salinity and pollution in Lake Qarun. So. this work aimed to investigate the histology and ultrastructure of the gills of Tilapia nilotica to clarify the possible effect of water quality on the fish gills. The most observed changes include the occurrence of a prominent large number of chloride and mucous cells confined to gill filament, hyperplasia of primary lamellar epithelium which leads to obliteration of some interJamellar spaces between adjacent secondary gill lamellae, odema resulting in the separation of respiratory epithelium and inflammatory infiltration including eosinophils and macrophages. These findings demonstrate that Tilapia nilotica can adapt to poor conditions through cellular and histological changes.
https://ejabf.journals.ekb.eg/article_1790_265c502b1ac8bc7bc3c0be4ecbe44074.pdf
2003-09-01
157
181
10.21608/ejabf.2003.1790
histology
ultrastructural. gilts
Tilapia nilotica
Lake Qarun
Hoda
Shoman
1
Zoology Department. Faculty of Science, Al-Azhar University for Girls, Nasr City, Egypt,
AUTHOR
Hanan
Gaber
2
National Institute of Oceanography and Fisheries, Fish Research Station, Egypt.
AUTHOR
ORIGINAL_ARTICLE
ECOLOGICAL IMPACTS OF USING CONTAMINATED FOOD IN THE BOTTOM TRAPS AND BARRIERS OF FISHES
Some fishermen use contaminated food in the bottom traps and barriers in the Nile branches to attract fish. Microbiological investigations showed that such food is highly contaminated by bacteria and fungi. The identified genera of bacteria in food material, water and skin lesions were Aeromonas, Pseudomonas* Bacillus and Actinomycetes. On the other hand, four species of fungi namely Aspergilhts flavus, Aspergillus lerreus, Fusarium sp. and Penicillium chrysogenum were isolated from the contaminated food. The field observations revealed that Tilapia zillii samples collected from the area contaminated by such food had higher ratio of skin diseases compared to other sites. The skin diseases in this site included skin ulcer (13.8%), fin erosion (16.9%) as well as b -u. ions (10%). The ratios of the skin ulcers (>15%) and fin erosion (>30%) of the total body area among male and female T. zillii from the contaminated site were elevated compared to other ratios. The seasonal variation of different skin diseases revealed that the maximum prevalence, ranging between 40.8% and 24.2%, were detected in summer and spring respectively. The GLM statistical analysis revealed that site and season had significant effects (p<Q.Q5) on the frequency of skin diseases. There was insignificant correlation between the areas of minimal and moderate skin ulcers (<10% and 10-15% respectively) and fin erosions (<I5% and 15-30%) with the different biological variables of T. zillii males and females (i?2 ranged between 0.01 and 0.22). However, the regression of severe skin ulcers (>15%) versus such biological variables showed that feeding intensity (FI), fish condition (k) and hepato-somatic index (HIS) of males decreased with increasing the area of skin ulcers, while TSI was not affected by this lesion. The trend was more obvious in case of females, where FI5 K, HIS and OS1 were negatively affected by the skin ulcers. On the other hand, FI and K of males and FI, K and OSI of females were inversely correlated with severe fin erosions (>30%). Laboratory investigations showed that the skin ulcers and fin erosions first appeared in the 5th day after the application of the contaminated food with or without water from site (B) in the aquaria. The mortality rate in fish, which were provided with the contaminated food with or without water from site (B), was 10 % at day'18th and 33.3 % at days 19th and 20th, reaching 100 % at 22nd day.
https://ejabf.journals.ekb.eg/article_1791_ad95dbd4f28bc0b10a2b92dabc0ce2b3.pdf
2003-09-01
183
209
10.21608/ejabf.2003.1791
Ecology
skin ulcers
fin erosion
Bacteria
fungi. Tilapkt zillii
Alaa
na-ei
1
Zoology Department, Faculty of Science, Al-Menufiya University, Shebeen Aikoom
AUTHOR
Mohamed
Shaaban
2
Botany Department, Faculty of Science, Al-Menufiya University, Shebeen Aikoom
AUTHOR
ORIGINAL_ARTICLE
SPECIES COMPOSITION AND SEASONAL POPULATION DYNAMICS OF THE BENHIC FAUNA IN KHOR KALABSHA, LAKE NASSER, EGYPT
Seasonal population dynamics of the benthic fauna and nature of sediment at Khor Kalabsha, Lake Nasser, have been investigated between October 1989 and September 1990. The benthic fauna in Khor Kalabsha was rich in density and biomass. They were dominated by aquatic insects followed by gastropod molluscs and oligochaete annelids. The khor sediments are sandy or silty sand at littoral localities (sand content 87.37%), forming sandy silt at the offshores (53.58% silt and 25.92% clay). The average annual density of benthic fauna at the 1 ittoral stations amounted to 10.292 organisms/m2, with 33.9 gm wet wt./ nr. The offshore stations harboured lower densities of benthic organisms that averaged 908 organisms/m2, with 4.0 gm wet wt./ m2. Aquatic insects constituted 85.1% by number (8757 insects/m2) at the littoral stations. These values declined to 63.0% (average = 574 insects/m2)-at the offshore stations. Benthic fauna attained their maximum persistence in winter while the spring and summer sustained lowest densities. Gastropod molluscs constituted 11.1% by number (1138 organisms/m2) at the littoral stations. Their occurrence at the offshore stations was very rare and constituted 6.6% by number (60 organisms/m2). Oligochaetes constituted 3.8% at the littoral stations. At the offshore stations, they formed numerically 30.4% of the total benthos. Spearman Correlation Coefficient revealed negative relationship between the density or biomass of benthic fauna at littoral stations and organic carbon, inorganic phosphorus and total carbonate. In contrast, a positive correlation was recorded for organic phosphorus. At the offshore stations, Spearman Correlation Coefficient indicated negative relationship between the density of benthic fauna and organic carbon, inorganic phosphorus and total carbonate. In contrast, a positive correlation was documented between the density and organic phosphorus as well as between the biomass and above mentioned physicochemical parameters.
https://ejabf.journals.ekb.eg/article_1792_34e219277fda5c1eee9e9c54b815e9df.pdf
2003-09-01
211
240
10.21608/ejabf.2003.1792
Lake Nasser
Khor Kalabsha
Benthic fauna
Seasonal dynamics
Sayed
EI-Tantawy
1
Zoology Department. Faculty of Sciences, Mansoura University. Mansoura. Egypt
AUTHOR
Reda
Bishai
2
Zoology Department. Faculty of Sciences, Mansoura University. Mansoura. Egypt
AUTHOR
Abu-EIfotuh
Abdel
3
National Institute of Oceanography and Fisheries, Egypt
AUTHOR
Ishak
Iskaros
4
National Institute of Oceanography and Fisheries, Egypt
AUTHOR
Amin
Samaan
5
National Institute of Oceanography and Fisheries, Egypt
AUTHOR
ORIGINAL_ARTICLE
VERIFICATION OF THE DELETERIOUS EFFECTS OF LERNAEOSIS ON THE HEALTH OF GRASS CARP (CTENOPHAR YNGODONIDELLA)
Experimental infestation of Ctenophuryngodon idella (80-90 g) with laboratory reared first copepodid stage of Lernaea cyprinacea (a parastitic copepod ) resulted in clinical lernaeosis. The mortality rate reached up to 40% of the infested group within <a month at 26 ± 2°C. The sequential clinical abnormalities, histolopathcrilogicai and hematological alterations with elevation in the cortisol lewd had verified experimentally the deleterious effects of lernaeosis* on the health of G idella. Periodical examination of the experimentally infested C. idella cleared that the females parasites were tlfrie most physically detrimental stage to-the. fisfcv withe regard to their ranode of attachment and cortisol response. Induction of lernaeosis in the present investigation may be important when attempting to develop useful effective protective and / or curative measures for lernaeosis in G idella and probably in other cyprinids.
https://ejabf.journals.ekb.eg/article_1793_8c0bf099d212423a55e738351b7e9a2b.pdf
2003-09-01
241
261
10.21608/ejabf.2003.1793
parasites. Lernaea cyprinacae
mortality
hematoilogy. histopathology
biological stress
cortisol. Ctenopharyngodon idella. experimental infestation
ORIGINAL_ARTICLE
REPORODUCTIVE PERFORMANCE OF OREOCHROMIS NILOTICUSTHROUGH THREE SEASONS IN EARTHEN PONDS
Reproductive performance of Oreochromis niloticus broodstock was examined in earthen ponds through three spawning seasons in Sedy Salm City, Kafer Al-Sheikh Governorate. 21 thousand females (65-150 g) and 10.5 thousand males {75-200 g) were randomly assigned to 10 ponds (750 m ) at a density of 600; 700 & 800 females and 300; 350 & 400 males per pond. Fish were exposed to different levels of water temperature ranging from 20.04 °C to 36.06 °C during the three spawning seasons.Oreochromis niloticus began to lay eggs at water temperature 20.06 "C. Spawning percentage increased during April; May and June were 23.82; 30.05 and 20.61 % at water temperature 20.04; 26.26 and 34.75 °C in three seasons respectivialy. During May, 2000, 2001 & 2002 female gave high number of fries /.m2/ day ; 7.85±1.73 ; 6.84±1.16 and 7.85±2.03 fries / m2 /day, respectivialy. Also, high female relative fecundity were 1.25±0.41; 1.76±0.24 and I.3±0.34 fries/g female body weight at three season, respectivialy. On the other hand, high fry production per spawn per female was 184.6i53.01; 149.26i31.87 and 143.26±25.37 fries / female, when water temperature was 20.04; 26.79 and 26.26 °C, respectivialy.It is evidant that the reproductive performance of Oreochromis niloticus could be maximized by holding broodstock under natural photoperiod in water temperature above 20°C, the optimal reproductive performance occurs through summer season during May. The optimal water temperature range was 28-31 °C and females with highest body weight started to spawn first and gave more total fries.
https://ejabf.journals.ekb.eg/article_1794_f5adb2070bc9bdcf89bba2e66dfab9e6.pdf
2003-09-01
263
282
10.21608/ejabf.2003.1794
reproductive performance
relative fecundity. Oreochromis niloticus
Water Temperature
earthen pond
Mohamed
Farag
1
Genetics Department, Central Laboratory for Aquaculture Research, Abbassa, Agricultural Research Center, Egypt.
AUTHOR
ORIGINAL_ARTICLE
SEASONAL VARIATIONS OF TRACE METALS LEVELS IN WATER AND THE LIMPET PATELLA CAERULEA OF ALEXANDRIA COAST, EGYPT.
The whole soft tissues of the Patella caendea from Abu-Qir, EI-Anfushi and El-Mex stations in Alexandria were seasonally analyzed during the period from Autumn 2001 to Summer 2002, to determine the levels of Cd, Co, Cu, Fe, Pb and Zn to reveal their patterns of accumulation throughout different seasons. The correlation between seasonal variations and the nature of investigated stations in metal concentrations was significant. Summer recorded the highest concentrations of Cu and Zn and the lowest one for Pb. Winter recorded the lowest concentrations of Fe, Zn and Cd. while Co recorded its highest concentration in the same season. Fe recorded its highest concentration in Spring, while Autumn recorded the highest concentrations for both Cd and Pb.On the other hand, Cd, Pb and Zn concentrations in water and tissues revealed highly significant differences for stations and seasons. Co, Cu and Fe concentrations in water revealed marked differences for both seasons and stations. There were no marked differences between seasons for Co, Cu and Fe that accumulated in the soft parts of Patella tissues, while stations were significant. The highest values of Cd and Pb was reported for samples collected from El- Mex in Autumn, while the highest ratio of Cu was revealed for the same station but in Summer. The highest concentration of Co appeared in Autumn from El-Anfushi, while its lowest concentration during the same season was in Abu-Qir. Fe recorded its highest concentration in Abu-Qir station in Spring. While El- Anfushi presented the highest concentration of Zn in Summer.
https://ejabf.journals.ekb.eg/article_1795_abf6aeb294a3b421ceab63dd0258e548.pdf
2003-09-01
283
312
10.21608/ejabf.2003.1795
trace metals
Seasonal variations
rocky shores
Patella caendea
Sabry
El-Serafy
1
Zoology Department, Faculty of Science, Zagazig University. (Benha branch). Egypt.
AUTHOR
Dalia
El-Sayed
2
Zoology Department, Faculty of Science, Zagazig University. (Benha branch). Egypt.
AUTHOR
Mona
EI-Gamal
3
ZooIogy Department, Faculty of Science. Tanta University. Egypt
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF ^TOCOPHEROL ACETATE ON FECUNDITY AND REPRODUCTIVE PHYSIOLOGY OF OREOCHROMIS NILOTICUS AND O. AUREUS BROODSTOCK.
Astudy was conducted to determine the effect of vitamin E, (a-tocopherol acetate a-TOH) supplementation on the fecundity and reproductive performance of Nile tilapia Oreochromis niloticus and O. aureus. R eproductive performance was assessed in the females, in the developing eggs and in the resultant offspring, in an attempt to determine the optimum nutrition for successful spawning of O. niloticus and O. aureus. The reproductive response of O. niloticus and O. aureus to alpha-tocopherol acetate was nearly the same regarding egg number and quality, hatchability, gonadosomatic index and quality of the resulting offspring,Results revealed that alpha-tocopherol acetate greatly improved not only egg and sperm quality but also seed production. None of the three levels of alpha-tocopherol acetate had any obvious effect on the mean relative fecundity of either O. niloticus or O. aureus. Females of both strains that fed on a diet supplemented with 50 mg «-tocopherol acetate/kg produced larger eggs than females of the control that fed on diet without supplemental alpha- tocopherol, or on diets supplemented with lower levels of a -TOH, The highest mean hatching percentage and lowest mean percentage of deformed larvae were observed in eggs extruded from females fed 50 mg a-TOH/kg-supplemented diet in both strains. While lower mean values of hatching percentage and higher mean values of deformed larvae percentage were detectable in eggs of those females that were fed supplemental 25 and 5-mg a-TOH/kg diet. Fry of the third treatment attained the highest mean body weight. The smaller sizes of fry were obtainable from eggs produced by females fed the lower levels of a-TOH. Fry of females that fed tocopherol -free diet was the smallest. Females that fed on diets provided with higher levels of <x-TOH not only produced larger eggs but also had higher mean values of GSI than those fed on diets with lower levels.Irrespective of the supplemental a-TOH levels that had no effect on the mean number of eggs/female in the three treatments of both strains, correlating the 0. niloticus and O. aureus female body weight and the corresponding number of eggs, produced, showed a linear correlation. The equation of this relationship in the first spawning is represented as follows:Y= 4.3353 X - 166.67 for <9. niloticus and Y = 4.7819X-235.76 for <9. aureus. where Y= number of eggs and X = female body weight.Further investigations are needed to estimate egg production/female in the following spawnings all over the reproductive period under the same conditions.With regard to the reproductive hormones, concentration of luteinizing hormone (LH) and follicle stimulating hormone (FSH) in serum of 0. niloticus females of the third treatment, that exhibited more active reproductive performance, was higher than that in serum of females of the other treatments and the control.
https://ejabf.journals.ekb.eg/article_1796_e2fd5a622b9da570cfdc60bf68786b3c.pdf
2003-09-01
313
330
10.21608/ejabf.2003.1796
Oreochromis niloticus
Oreochromis aureus
fecundity
reproductive physiology
nutrition
ORIGINAL_ARTICLE
INFESTATION LEVEL AND SPATIAL DISTRIBUTION OF PROTOANCYLODISCOIDES MANSOVRENSIS EL-NAGGAR 1987, A MONOGENEAN GILL PARASITE FROM THE LONG FIN CATFISH CHRYSICHTHYSAURATUS GEOFFROY, 1809
Atotal of 110 specimens of the Nile catfish Chrysichlhys awalus were caught from Damietta Branch of the River Nile during spring and summer 2002 and 2003 and surveyed for monogenean gill parasites. Only a single highly host specific monogenean, namely Protoancylodiscoides mansourcnsis El-Naggar, 1987 was encountered. Out of the fish examined. 2224 monogenean worms were recovered. The parasite showed an aggregated or contagious pattern of distribution within the host population. The parasite attained high prevalence (97,27 %). abundance (20.22± 21.74) and mean intensity (20.79 ± 22.03) values. The parasite preferred larger hosts than smaller ones. Spearman Correlation Coefficient showed a positive relationship between host length or weight and intensity of/5. mansourensis. The skeletal support of the holobranch is provided with a powerful sieve or a complex trapping system, being formed of interlocking epidermal semi circular folds alternated with fold-free interspaces. Several gill filaments were branched once, twice and even three times to produce additional filaments, probably to increase the gas exchange capacity in the oxygen-poor bottom of the river bed. The present model showed marked microhabitat availability as hundreds of thousands of attachment sites (each comprising two adjacent gill lamellae and an interlamellar channel) accessible for monogenean infestation were calculated on the host gills. The distribution of the parasite on the gills was non-random. The parasite showed a gradual decline in the percentage distribution from the first (outermost) to the fourth (innermost) gill arch (holobranch) as well as from the dorsal to the ventral gill segments. The parasite preferred the proximal half of the gill filaments over the distal. However, there was no significant difference in the percentage distribution of P. mansouremls between the left and right hand gill sets or between the anterior and posterior hemibranchs. Factors contributing to the microhabitat preference of the parasite on the gill map and those regulating its population growth among host individuals of different length or weight classes are discussed in detail.
https://ejabf.journals.ekb.eg/article_1797_409d03b9dc41f47856f295784830b416.pdf
2003-09-01
331
357
10.21608/ejabf.2003.1797
Chrysichlhys aural us\ Protoancylodiscoides mansourensiw Monogenea. infestation level
microhabitat distribution
Ahmed
El-Naggar
1
Zoology Department, Faculty of Sciences, Mansoura Univ Mansoura, Egypt
AUTHOR
Enayat
Reda
2
Zoology Department, Faculty of Sciences, Mansoura Univ Mansoura, Egypt
AUTHOR
ORIGINAL_ARTICLE
MODE OF ATTACHMENT OF THE MONOGENEAN PROTOANCYLODISCOIDES MANSOURENSIS EL-NAGGAR 1987 TO GILLS OF THE LONG FIN CATFISH CHRYSICHTHYS AURATUS,V/ITH REFERENCE TO HOST-PARASITE INTERFACE
The mode of attachment of the ancyrocephaline monogenean Protoancylodiscoides mcmsourensis to the gills of the long fin catfish Chrysichthys auratus has been investigated with reference to host-parasite interface. Light and scanning electron microscope observations revealed that the vast majority of worms secured themselves between two adjacent gill lamellae at the area opposite to the efferent or afferent side of the giil filament or at the middle area between the two sides, within the interlamellar space or channel. Frequently, the whole parasite was observed to hide behind the gill lamellae. The parasite preferred clinging to mucous cell-poor microhabitats such as proximal sector of the gill lamellae and avoided mucous cell-rich microhabitats such as the distal region of the gill filament and gill lamellae as well as the uppermost layer of interlamellar epithelium. The worm utilized one pair of large dorsal hamuli and another pair of comparatively small ventral hamuli to attach firmly on the host tissue. However, marginal booklets had no role in attachment. The local histopathological impacts of the parasite on the gill tissues comprised compression and even tearing of the covering epithelium of the affected gill lamella, aggregation of blood corpuscles in the vicinity of haptoral attachment and change in the distribution pattern of different cell types of the affected gill lamella. A few number of lamellae became amorphous. The host response to Protoancylodiscoides infestation comprised gill tissue proliferation (hyperplasia), formation of sinus-like blood collection and occasional appearance of lymphocytes and/or mucus-producing cells in the vicinity of haptoral attachment. Ecological significance of the mode of attachment and selection of highly protected or sheltered microhabitats and interaction between infested gill tissues and invading parasites are discussed in detail.
https://ejabf.journals.ekb.eg/article_1798_3d0cc2ba06475b2467d68c024f04a45c.pdf
2003-09-01
359
380
10.21608/ejabf.2003.1798
Chrysichthys entrants. Protoancylodiscokfes mcmsourensis. Monogenea
mode of attachment. local impacts
host response
Enayat
Reda
1
Zoology Department. Faculty of Sciences. Mansoura University. Mansoura. Egypt
AUTHOR
Ahmed
El-Naggar
2
Zoology Department. Faculty of Sciences. Mansoura University. Mansoura. Egypt
AUTHOR
ORIGINAL_ARTICLE
COMPARATIVE STUDY OF SOME ANTIBIOTICS ON BIOCHEMICAL CHANGES IN PLASMA OF THE SHARPTOOTH CATFISH CLARIAS GARIEPINUS.
Ciprofloxacin (15 mg/kg body weight), amoxycillin (50 mg/kg body weight) and ampicillin (80 mg/kg b. wt.) administered orally to male sharptooth catfish Glorias gariepimts 3 times, every 72 hours intervals. Plasma samples were taken for three successive days after the last dose the most important results were :t) Plasma glucose levels were significantly elevated in fish administered ciprofloxacin and amoxycillin at the 3 days studied and ampicillin at the 3rd day. In contrast, hypoglycemia observed in the first two days in plasma of C. gariepinus administered ampicillin. 2) Significant hyperproteinaemia was observed in fish groups administered bertfer etpfefkweaein and amoxycillin antibiotics during the three days studied. Ampicillin showed a significant highest value at the second day, while the values at both 1st and 3rd days showed significant lower values. 3) Administration of the three antibiotics significantly raised the plasma urea in the three days studied except the 2nd and 3rd days in fish administrated amoxycillin and ampicillin respectively. Also, increased plasma concentrations of uric acid and creatinine in C. gariepinus administered the three antibiotics at the three days studied were recorded. 4) The AST and ALT activities in C. gariepinus administered both ciprofloxacin and amoxycillin antibiotics showed significant higher values at the 3rd day. The slope of rise of AST and ALT activities in both ciprofloxacin and amoxycillin were greater than that of ampicillin that is approximately similar to the control group. So, ciprofloxacin, amoxycillin followed by ampicillin are effective on the liver functions in a decreasing order. This observation is clear in ALT than AST, which indicates that ALT is more sensitive to antibiotic administration. 5) Analysis of ALP activity in all groups of C. gahepinus administered with the three antibiotics in the three periods studied showed significant higher values. The administration of antibiotic may considered as stress factor in the fish even when it used in the prophylactic dose. Glucose and ALT, AST and ALP can used to evaluate the stress condition in fish especially in the farm condition.
https://ejabf.journals.ekb.eg/article_1799_7e4580ef155909172415b4204dc7624e.pdf
2003-09-01
381
402
10.21608/ejabf.2003.1799
catfish
Cfarias gariepinus
ciprofloxacin
amoxycillin. ampicillin
Plasma Glucose
Total protein
urea
uric acid. creatinine
transaminases
alkaline phosphatase
Esam
Rizkalla
1
Biochemistry, Nutritional Deficiency Diseases and Toxicology Department, Animal Health Research Institute, Dokki.
AUTHOR
Basem
Fahmy
2
Biochemistry, Nutritional Deficiency Diseases and Toxicology Department, Animal Health Research Institute, Dokki
AUTHOR
Hany
Zaghlou
3
Pharmacology Department. Fac. Vet. MedicineCollage, Bani-Suife division, Cairo University.
AUTHOR
Salah El-Din
El-Sadek
4
Pharmacology Department. Fac. Vet. MedicineCollage, Bani-Suife division, Cairo University.
AUTHOR
ORIGINAL_ARTICLE
HEAVY LMETALS ACCUMULATION AND MACRONUTRIENTS IN THE LIVERS OF SOME FISH SPECIES OF BARDAWIL LAGOON AND THEIR HISTOLOGICAL CHANGES
Fish samples of Solea vulgaris. Mugil cephalus, Liza ramada and Spams aurata were collected during November 2001 from El Telol location in Bardawil lagoon, north eastern of Egyptian Mediterranean coast. The study was conducted to assess the effect of the heavy metals (Fe. Mn. Zm Cm Pb and Cd) and the macro nutrients (Na, K, Ca, and Mg) on livers tissues of the four fish species. The results showed that the concentrations of Fe, Mn, Zn, Cu, Pb, and Cd in the livers of the four fish species ranged between 48.680- 121.940, 1.270* 2.170. 15.220- 32.570. 82.280- 30L830. 1.100- 4.960 and0.180- 0.300 mg/ Kg respectively. The concentrations of Na, K. Ca and Mg in the livers of the four species ranged between 1131.60- 1702.00, 327.60- 507.00, 74.490- 398.300 and 72.180- 170.590 mg/Kg respectively. Microscopical examination of livers tissues of Solea vulgaris, Liza ramada* Mugil cephalus and Spams aurata showed different degrees of injuries, including fatty degeneration, ballooning degeneration, accumulation of hemosiderin pigment and necrosis.
https://ejabf.journals.ekb.eg/article_1800_3c2f0c4465b07611db299657f7c7896e.pdf
2003-09-01
403
422
10.21608/ejabf.2003.1800
Bardawil Lagoon
fish
Liver
heavy metaL Histopatholgy
Amal
Yacoub
1
National Institute of Oceanography and Fisheries, El Kanater El Khairya Fish Research Station, Cairo, Egypt.
AUTHOR
Amal
Abdel Satar
2
National Institute of Oceanography and Fisheries, El Kanater El Khairya Fish Research Station, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
GENERAL SURVEY ON CERTAIN HELMINTH PARASITES INFECTING SOME NILE FISHES AT EL-MANSOURA, EGYPT.
The present investigation deals with certain helminth parasites which infect the freshwater fishes in the Nile River and its common tributaries at Dakahlyia Governorate near El-Mansoura city, Egypt.The examined fishes were identified as Bagrvs hajad ; B.docmac; Synodontis schail: S. sen-aim; Mormyrus caschive; Barbus bynni; Lates mloticus and Labeo niloticm. These fishes were investigated monthly after weighing and measuring them and most of their internal organs were examined for helminth parasites.The following trematodes were recorded: Acamthodtomvm absconditum; Haplorchoides cahirinns; Phyllodistomum aegyptiacus; metacercariae of Diplostomum sp ; of Neodiplostomum sp and of Posthodiplostomwn sp. Moreover, two nematode species were reported, namely: Spinitectus moraveci and Capillaria yamagutii. Meanwhile, no cestode parasites were recorded in this study.Out of 400 collected fishes, 349 were found to harbour one or more types of parasites. The general infection rate was 87.25%. The infestation rate was highest in Bagrus baiad (97.7%) followed by Bagrus docmac (93.33%), while the lowest rate was recorded in Barbus bynni {69.1%).It was noticed that single infection was recorded for trematodes and nematodes in hostfishes. Double infection was not detected in Barbus bynni and was very rare in other fishes except B. hajad and B. docmac.The relation between fish host parameters (body length, weight and sex) and each of the prevalence and intensity of infection were studied in the present investigation. A significant correlation was found between the host length and infection prevalence, between the host weight and prevalence and between the host sex and intensity of infection, while insignificant relation was recorded between the host length or weight and intensity; and between host sex and prevalence of infection,
https://ejabf.journals.ekb.eg/article_1801_d27032582e8d172368a79dad5e8bdda1.pdf
2003-09-01
423
446
10.21608/ejabf.2003.1801
helminth parasites
trematodes
Nematodes
River Nile
Freshwater fishes
Mohamed
Mansour
1
Zoology depU Fac. of Science, Mansoura Univ., Egypt
AUTHOR
Sameh
Hassan
2
Zoology depU Fac. of Science, Mansoura Univ., Egypt
AUTHOR
Abd El-Aziz
Khidr
3
Zoology depU Fac. of Science (Damitta), Man soura Univ., Egypt.
AUTHOR
Mosad
Ghanem
4
Zoology depU Fac. of Science (Damitta), Man soura Univ., Egypt.
AUTHOR
ORIGINAL_ARTICLE
ACCLIMATION OF DIFFERENT POSTLARVAL STAGES OF PENAEUS KERATHURUS (FROSKAL, 1775) TO LOWER SALINITY LEVELS
Lower salinity acclimation of hatchery-reared Penaeus keruihurus postlarvae was investigated in short and long term experiments at PL20, PL30, PL40 and PL50. In short term experiment, the salinity tolerance was determined with/without acclimation. In tolerance test without acclimation, PLs were transferred suddenly from 36 ppt to 2, 5, 10, 20 and 30 ppt. In tolerance test with acclimation, PLs were acclimated over four days from the reared salinity (36 ppt) to 2, 5, 10 and 20 ppt. After acclimation, PLs were transferred from acclimated salinities to lower and higher salinities. In both tolerance testes, PLs were kept for 96 hr. It was found that acclimation has a significant effect in some cases at PL30 and PL40. In both tolerance tests, the highest salinity tolerance (80-90 % survival rate) was attained at PL50 in salinity range of 10-36 ppt. In long term growth experiment. PLs were reared at 5. 10, 20 and 36 ppt for 30 days using minced clam meat. The highest specific growth rate (SGR), survival and production were recorded at the salinity range.of 10-20 ppt.
https://ejabf.journals.ekb.eg/article_1802_40594e68148f1d30fe6bd6d96ddab526.pdf
2003-09-01
447
463
10.21608/ejabf.2003.1802
Acclimation
salinity. Penaeus kerafhurus
post-larval staples
Ragia
Moussa
1
National Institute of Oceanography & Fisheries. Kayed Bay. Alexandria, Egypt
AUTHOR
Somaya
Taha
2
National Institute of Oceanography & Fisheries. Kayed Bay. Alexandria, Egypt
AUTHOR
ORIGINAL_ARTICLE
RESTRICTION FRAGMENT LENGTH POLYMORPHISMS(RFLPs) OF THE SMALL-SUBUNIT RIBOSOMAL DNA AS A TOOL FOR IDENTIFICATION OF TILAPIA SPP.
In Egypt, four species of Tilapia have been described based on morphometric, meristic and cytotaxonomical characteristics. These species are Tilapia zillii, Oreochromis niloticus, Oreochromis aureus and Sarotherodon galilaeus. The accurate identification of these fishes is complicated by the high variation in these characters,similarity among species and in some cases by the size of the fish. In this paper, we examined the use of polymerase chain reaction (PCR)and restriction fragment length polymorphisms (RFLPs) analysis of the nuclear small subunit ribosomal RNA gene (srDNA) for molecular identification of Tilapia spp. in Egypt. The present study aims to evaluate such advanced molecular biological approach for identification of Tilapia spp. Genomic DNA was extracted from the four species of Tilapia. About 2000 bp 18S ribosomal DNA was amplified by PCR using specific primers. The technique of restriction fragment length polymorphisms was used to identify the specific 18S rDNA for each species. O. niloticus rDNA 'RFLPs species-specificpattern is proved by digestion with restriction endonucleases AIwNI and Aval, On the other hand, digestion of amplified 18S rDNA with endonuclease enzymes Smal, Xmal and Sstll produced speciesspecific patterns for T. zillii, O. aureus and S. galilaeus respectively. This indicates the efficiencies of these restriction endonuclease enzymes in species-specific identification of Tilapia spp. Restriction fragment length polymorphisms of the nuclear srDNA (RFLPs\PCR)proved to be a good tool for checking the relationships among species and their subspecies, even more than the morphological analysis.
https://ejabf.journals.ekb.eg/article_1803_5b79a34ebbf4488311e3b3e01c1f959c.pdf
2003-09-01
465
482
10.21608/ejabf.2003.1803
identification
tilapia
nsrDNA
RFLPs. PCR
Polymorphism
Sabry
El-Serafy
1
Department of Zoology, Faculty of Science, Zagazig University, Benha Branch
AUTHOR
Mohammed
Awwad
2
Department of Zoology, Faculty of Science, Zagazig University, Benha Branch
AUTHOR
Mona
Azab
3
Department of Zoology, Faculty of Science, Zagazig University, Benha Branch
AUTHOR
Nasr-Allah
Abd- EI-Hameid
4
Department of Zoology, Faculty of Science, Zagazig University, Benha Branch
AUTHOR
ORIGINAL_ARTICLE
HISTOLOGICAL ANOMALIES IN GONADS OF OREOCHROMIS NILOTICUS (CICHLIDAE) FEEDING ON ETHYNYLTESTOSTERONE AND ACTIVE YEAST.
Male-population of Tilapia nilotica was produced with 17aethynyltestosterone at 60ug/g diet. Subsequently fry were fed1.2,3,4 and 5 g/kg active yeast diet for 70 days. Fish fed on dietary yeast were significantly heavier than the control. The percentage of males was 62.1. Ovotestis condition was found. Histological anomalies of the gonads of both sexes have been observed.Sterilization of the testis was complete at all levels of yeast diet.Dietary treatment had a destructive effect on the histological appearance of gonadal development.
https://ejabf.journals.ekb.eg/article_1804_baa547c37ecf893948c5f5d641c7a4d9.pdf
2003-09-01
483
501
10.21608/ejabf.2003.1804
histological anomalies
Gonads
ethynyltestosterone.active yeast
Samia
MOHARRAM
1
National institute of Oceanography & Fisheries - Alexandria
AUTHOR
EL-Said
Ebiary
2
National institute of Oceanography & Fisheries - Alexandria
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF EUPHORBIA PEPLUS PLANT EXTRACT AND THE ANTIHELMENTHIC PRAZEQUANTEL ON THE DEFENCE SYSTEM OF BIOMPHALARIA ALEXANDRIA SNAIL
The effect of sublethal concentrations of£ pephis water suspension and praziquantel water solution on the defense system of Biomphalaria cdexandrina was examined by laboratory experiments.The mean number of haemocytes decreased insignificantly after the first week of snail exposure to the plant extract- Thereafter, a significant increase occurred after the second week of exposure. Treatment with praziquantel showed a significant increase in haemocytes after the 3rd and 4lh weeks of exposure. Haemocytosis represents a response to external stress or certain stimuli. The haemocytes showed behavioral as well as structural changes after long exposure to the tested mollusclcides. The cells had many cytoplasmic inclusions and produce many long spike-like pseudopodia. Haemocytes exhibited an enhanced tendency to aggregate in clusters. Such alterations may be indicative of a state of haemocytes activation. The total plasma protein concentration after treatment with E. pephis and Praziquantel increased plasma than in control snails while no changes in protein concentration of the digestive gland extract. This may be a result of an imbalance in the rate of protein synthesis and the rate of its degradation.The treatment with E peplus and Praziquantel caused a marked increase in acid phosphatase after the 2nd and 4lh weeks. On the other hand, alkaline phosphatase activity increased after the 4th week. This could be explained as defensive and lysosomal activity of the haemocytes. After treatment with the tested substances the plasma and digestive gland extract were capable to agglutinate sheep red
https://ejabf.journals.ekb.eg/article_1805_d3c1ab5616b02c49d299b86f8d1be80e.pdf
2003-09-01
501
527
10.21608/ejabf.2003.1805
Plant moiluscicide ^Euphorbia pephts* Praziquantel
Haemocytes
defence system
B
alexcmdrina
Ibrahim
Helal
1
Zoology Department, Faculty of Science. Tanta University, Tanta, Egypt*
AUTHOR
Mostafa
ELMehlawy
2
Zoology Department, Faculty of Science. Tanta University, Tanta, Egypt*
AUTHOR
ElSayed
Rizk
3
Zoology Department, Faculty of Science. Tanta University, Tanta, Egypt*
AUTHOR
Gehan
EL-Khodary
4
Zoology Department, Faculty of Science. Tanta University, Tanta, Egypt*
AUTHOR
ORIGINAL_ARTICLE
بالبحر الأحمر في المملکه العربيه السعوديه Siganus rivulatus بالخليج العربي وأسماک السيجان Siganus canaliculatusدراسه مقارنه علي أسماک السيجان
https://ejabf.journals.ekb.eg/article_1806_815e38f98da28bf06ccfb17bbb2d0032.pdf
2003-09-01
1
27
10.21608/ejabf.2003.1806