ORIGINAL_ARTICLE
Molecular and biometric characterizations of the Western king prawn Melicertus latisulcatus (Kishinouye, 1896) in the Egyptian Red Sea.
Although the western king prawns Melicertus latisulcatus (Kishinouye, 1896) are among the highly-priced shrimp species in the world, there is a great shortage in research dealing with both morphological and molecular clues for the identification of these species. Therefore, the morphological study was designed to provide a full morphological description of this important species. The morphometric analysis proved the variability of the relative growth between sexes suggesting that length-weight relationships could be used as important criteriafor selective breeding programs. For molecular identification DNA was extracted from the samples to provide genetic barcodes of this species using polymerase chain reaction (PCR)-amplification of 16SrRNA and cytochrome oxidase subunit 1 (COI) genes. The results clearly showed that, the Egyptian M. latisulcatus speciesis genetically closely related toall Melicertus species, while both barcoding and phylogeny of this species resulted in clear species discrimination. Furthermore, the samples identified in the current study seem to belong to a single origin, differing from those from other geographical locations where the species was found.Overall, this study suggested a valuable tool for assessment of prawn populationsat both fisheries and genetic levels, providing a toolfor managementas well as for the optimization of its aquaculture.
https://ejabf.journals.ekb.eg/article_7181_c4dbff2f3147cc1fb0ae5caefea280d5.pdf
2018-01-01
1
14
10.21608/ejabf.2018.7181
Melicertus latisulcatus
Red Sea
Biometrics
Genetics barcodes
molecular study
Eman
Abbas
emanabbas03@yahoo.com
1
Genetic and Genetic Engineering Lab, Aquaculture Division, National Institute of Oceanography and Fisheries (NIOF), Qaitbay, Alexandria, Egypt.
AUTHOR
Khaled
Geba
khaled.mohammed@icman.csic.es
2
Genetic Engineering and Molecular Biology Division, Faculty of Science, Menoufia University, Egypt.
AUTHOR
Zaki
Sharawy
zaki_sharawy@yahoo.com
3
invertebrates Lab, Aquaculture Division, National Institute of Oceanography and Fisherires
LEAD_AUTHOR
ORIGINAL_ARTICLE
Phylogenetic relationships and taxonomy of three species of family Lethrinidae in the Red Sea, Egypt
Species of family Lethrinidae are of a great commercial importance in Egypt. Recent researches proved that we are far from having a full list of the family species diversity in Egypt. The purpose of this study was to clarify the phylogeny of commercial fish species of family Lethrinidae in the Egyptian Red Sea. Three commercial species of Lethrinidae (Lethrinus mahsena, Lethrinus nebulosus and Monotaxis grandoculis) were obtained from northern Red Sea proper. Samples were identified based on morphological characters. Identification was confirmed using DNA barcoding technique. DNA extraction was performed using Phenol-chloroform extraction method. In PCR, About 655 bp were amplified from the 5' region of the COI gene from mitochondrial DNA, then successful amplifications were sequenced. By Combining our sequence results with sequences of some species of Family Lethrinidae submitted to the GenBank, phylogenetic trees were constructed using p-distance Neighbor-Joining method. Results indicated that the use of DNA barcoding technique for emperor fish and big eye bream identification was successful, not only between different species of the family, but also within species, indicating the possible presence of cryptic species complex and the possible discovery of new genetic diversity. Of the three examined species from the Egyptian coasts; Monotaxis grandoculis showed the lowest genetic differences. Lethrinus mahsena showed the highest variations.
https://ejabf.journals.ekb.eg/article_7710_2f1bc459d11db91825abb4ddd2a0e85a.pdf
2018-03-01
17
24
10.21608/ejabf.2018.7710
Lethrinidae
Phylogeny
DNA barcoding
Taxonomy
Red Sea
Abdelmeneam
B. E.
1
Marine Science Department, Faculty of Science, Port Said University, Port Said, Egypt
AUTHOR
Ahmed, M. I.
Ahmed, M. I.
2
Marine Science Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
AUTHOR
Madkour F. F.
Madkour F. F.
3
Marine Science Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
AUTHOR
Hanafy M. H.
Hanafy M. H.
4
Marine Science Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
AUTHOR
ORIGINAL_ARTICLE
Assessment of heavy metals concentration in water, sediment and fish under different management systems in earthen ponds
The study was conducted in two fish farming systems in earthen ponds located in North- Nile Delta, Egypt. The first system was the feed fish farm that depended on pelleted diet (25% crude protein) as feed input, while the second system was the fertilizer fish farm which used both organic fertilizer and crushed macaroni as supplementary feed. Each system was replicated in four ponds (8400m2 each). The experimental period lasted from July 2014 to June 2015, including four months overwintering. Nile tilapia (Oreochromis niloticus), mullet (Mugil cephalus) and catfish (Clarias gariepinus) were cultured in each pond at initial weight of 2, 30 and 5 grams, respectively. Water, sediment and fish samples were collected to detect the heavy metals concentration. The results showed that heavy metals concentration in water, sediment and three organs of fish species were significantly (P<0.05) different between the feed and fertilizer fish farms. The pond management affected the content of heavy metals in water, sediments and fish. Accumulation of iron in water, sediment and three organs of fish were higher than other elements. Accumulation of cadmium and lead in water, sediment and three organs of fish were less than other elements. Accumulation of heavy metals in liver and gills were higher than that of muscles in all fish species. Metals concentrations in the edible part of farmed fish were within the permissible level for human consumption proposed by various international standard organizations.
https://ejabf.journals.ekb.eg/article_7704_3e7698fa97c5feccab01c5d2f1db9197.pdf
2018-06-03
25
39
10.21608/ejabf.2018.7704
pond management
fish farming
Heavy metals
Aquatic environment
fertilizer
I. M.
Shaker
1
AUTHOR
M. A.
Elnady
2
AUTHOR
R. K.
Abdel-Wahed
3
AUTHOR
M. A. M.
Soliman
4
AUTHOR
ORIGINAL_ARTICLE
Meristic and Morphometric Characteristics of five-lined snapper, Lutjanus quinquelineatus (Bloch, 1790) from the Red Sea, Egypt
In the present study, 120 fish specimens (64 male and 56 female) of the five-lined snapper, Lutjanus quinquelineatus of variable sizes were used for demonstration the morphometric and meristic characteristics of this species in the Egyptian Red Sea, Hurghada fishing area. The total length varied from 15.3 to 28.7 cm in males and from 16.0 to 31.7 cm in females while their weights ranged between 44 and 378 g in males and between 48 and 512 g in females. This fish species showed constant meristic characters. Statistical interpretation of morphometric data indicated that there is direct relationship between total body length with head length (HL), snout length (SnL), eye diameter (ED) and length of caudal peduncle (CPL). The meristic characters like dorsal fin rays, anal fin rays, lateral line scales, gill rakers on lower arm and scales in transversal line were counted. The results revealed that there is no sexual dimorphism in L. quinquelineatus from Hurghada fishing area.
https://ejabf.journals.ekb.eg/article_7723_a0de0e6f4c56d1b7fa6fc0cfc3d853df.pdf
2018-05-05
41
48
10.21608/ejabf.2018.7723
Lutjanus quinquelineatus
Morphometry
Meristic characters
Snapper
Red Sea
Egypt
Faiza M.
Soliman
1
AUTHOR
Sahar F.
Mehanna
2
AUTHOR
Hamdy A.
Soliman
3
AUTHOR
Taha S.
Baker
4
AUTHOR
ORIGINAL_ARTICLE
Impact of Fish- farming Management on Water Quality, Plankton Abundance and Growth Performance of Fish in Earthen Ponds
The study was performed in earthen ponds situated in North- Nile Delta, Egypt and adopted two fish farming systems. The first system was the feed fish farm that depended on pelleted diet (25% crude protein) as feed input, while the other system was the fertilizer fish farm which utilized both organic fertilizer and crushed macaroni as supplementary feed. Each system had four replicate ponds (8400 m2 each). The experiment duration was one year, including four months of over-wintering. Nile tilapia (Oreochromis niloticus), mullet (Mugil cephalus) and catfish (Clarias gariepinus) were cultured in each pond at initial weight of 2, 30 and 5 grams, respectively. Samples were collected to determine water quality, plankton abundance and fish growth performance. The results showed that the fertilizer fish farm had significantly high levels of pH, dissolved oxygen and total nitrogen content (TAN, NH3, NH4, NO2 and NO3) (P<0.05). Significant increase (P<0.05) in phytoplankton and zooplankton abundance was observed in the fertilizer fish farm compared to the feed fish farm. Within different species, catfish had the highest daily weight gain followed by tilapia and mullet, respectively. Within the two compared systems, farmed fish in the feed farm had higher average daily weight gain and specific growth rate. The feed fish farm had significantly (P<0.05) higher production and higher total income, while the fertilizer fish farm had higher net income and lower total production costs.
https://ejabf.journals.ekb.eg/article_7705_62e605c8c2d611a223085be11ff87319.pdf
2018-05-30
49
63
10.21608/ejabf.2018.7705
Aquaculture
Management
Growth performance
Organic fertilizer
Plankton
water quality
R. K.
Abdel-Wahed
1
AUTHOR
I. M.
Shaker
2
AUTHOR
M. A.
Elnady
3
AUTHOR
M. A. M.
Soliman
4
AUTHOR
ORIGINAL_ARTICLE
Seasonal growth of Arabian scad Trachurus indicus from the Gulf of Suez, Red Sea, Egypt.
Horse mackerel and scads, family Carangidae, are the most abundant pelagic fishes inhabiting the Gulf of Suez, Red Sea where they constituted about 77% of the total purse-seine catch in the Gulf. Growth of fish is a process that influenced by the environmental conditions especially temperature. Age and seasonal growth of Trachurus indicus were studied from samples collected randomly during the fishing season 2015/2016 from the Attaka port the main landing site in the Gulf of Suez. Otoliths of 1120 specimens were used for age determination and the maximum attained age for this species was three years. The seasonal length-weight relationship was investigated and the growth type was found to be greatly affected by environmental parameters in different seasons. The seasonal von Bertalanffy growth model was applied to estimate the seasonal growth parameters L∞, K and to. It is clear that temperature is a major factor affecting the Arabian scad growth.
https://ejabf.journals.ekb.eg/article_7715_a60a26119a23c883051a88cea3f2ca15.pdf
2018-05-01
65
73
10.21608/ejabf.2018.7715
Trachurus indicus
Arabian scad
Growth
Gulf of Suez
Red Sea
Egypt
Sahar
Mehanna
sahar_mehanna@yahoo.com
1
1- Fisheries Division, National Institute of Oceanography and Fisheries, Egypt
AUTHOR
Belal
Soliman
ba.soliman@yahoo.com
2
Faculty of Science, Suez University, Suez, Egypt
AUTHOR
Mohamed
Sayed
drmasayed08@gmail.com
3
Fisheries Division, National Institute of Oceanography and Fisheries, Egypt
AUTHOR
Rana
Abdel Aliem
4
Faculty of Science, Suez University, Suez, Egypt
AUTHOR
ORIGINAL_ARTICLE
Can intraspecific variations be exploited to develop salinity tolerance of Oreochromis niloticus?
Intraspecific variation of Oreochromis niloticus (Nile tilapia) to salinity tolerance was used through adaptation, selectivity to obtain the optimal O. niloticus tolerates salinity in which reproduction, growth, and metabolic rates will not be significantly affected. Three experiments have been conducted; in the first, the salinity tolerant individuals were selected and the survival rate wasdetermined in different salinity degrees from 6 to 36ppt. In the second experiment, the selected parents, (from the first experiment) were put in serial concentrations of salinity from 6ppt (as control) to 26ppt to determine their spawning ability. In the third experiment, fries of selected parents were reared in different salinity degrees, 6ppt (as control), 12ppt, 16ppt and 20ppt. The rearing period of fries of O. niloticus continued for 84 days. Chemical body composition of the fish and metabolic efficiency were determined at the end of rearing period. There was increase in the mortality ratio by increasing the salinity. Reproduction efficiency decreased with increasing salinity. The fish chemical body composition showed increasing in dry matter, ether and ash while crude protein decreased by increasing salinity. The growth performance increased gradually with increasing salinity concentration from 6 to 16ppt. Optimum salinity of weight gain in O. niloticus was at 16ppt, while 20ppt concentration was the lowest weight gain. In conclusion, the brackish water at 16ppt can be used for rearing O. niloticus without affecting growth rate and reproduction with adequate balanced nutrition.
https://ejabf.journals.ekb.eg/article_7719_482c661c3bbcdcf066279fca0c98303c.pdf
2018-05-05
75
85
10.21608/ejabf.2018.7719
Oreochromis niloticus
salinity tolerance
Intraspecific variation
Growth performance
Reproduction efficiency
Ahmed
El leithy
1
National Institute of Oceanography and Fisheries (NIOF), Qaitbay, Alexandria, Egypt.
AUTHOR
nermin
ibrahim
nerminabdelaziz2017@gmail.com
2
national institute of oceanography and fisheries
LEAD_AUTHOR
Zeinab
Helmy
zeinab_helmy7@yahoo.com
3
National Institute of Oceanography and Fisheries (NIOF), Qaitbay, Alexandria, Egypt
AUTHOR
Safaa
El Deeb
4
National Institute of Oceanography and Fisheries (NIOF), Qaitbay, Alexandria, Egypt.
AUTHOR